• Korean Journal of Microbiology
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• v.32 no.3
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• pp.202-207
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• 1994

A mutant strain PRM1 defective in light-activated heterotrophic growth was isolated from Synechocystis sp. PCC 6803. PRM1 could be grown at growth rate equivalent to Synechocystis 6803 under mixotrophic growth conditions. However, PRM1 could not be grown under light-activated heterotrophic conditions, in which a daily pulse of light for 5 min was given. These results suggest that PRM1 is not defective in heterotrophic metabolism, but in the transduction pathway of light signal essential to the growth. Plasmid patterns, absorption spectra of whole cells, and the exterior and interior structures of PRM1 were similar to those of Synechocystis 6803, except that PRM1 could not produce amorphous slime holding cells together.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.24 no.4
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• pp.197-215
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• 2013

This study generated the suggestions to minimize the duplicated cultural programs offered by various regional public service institutions via promoting effective collaboration among the institutions with the limited local resources while making the public libraries as the center of the partnership. We measured the level of program duplicability; conducted statistical analysis of the cultural program offering; and analyzed program participation status as well as the participation surveys. This led to the following results: 1) public libraries focused on offering humanities general education related programs; 2) participants had high expectations on the reading and writing programs; and 3) social welfare organizations offered programs, which targeted the older population, and the corresponding participants had high satisfaction rate. According to the results, the institutions including the public libraries should take into account of the ages and preferences of the potential participants when offering various programs. In addition, the public libraries should continue to offer humanities general education as well as information use programs.

• Journal of KIISE:Software and Applications
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• v.31 no.1
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• pp.8-19
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• 2004

In this paper, we propose an efficient method for locating destination address block on both of machine-Printed and handwritten Korean mail piece images. The proposed method extracts connected components from the binary mail piece image, generates text lines by merging them, and then groups the text fines into nine clusters. The destination address block is determined by selecting some clusters. Considering the geometric characteristics of address information on Korean mail piece, we split a mail piece image into nine areas with an equal size. The nine clusters are initialized with the center coordinate of each area. A modified Manhattan distance function is used to compute the distance between text lines and clusters. We modified the distance function on which the aspect ratio of mail piece could be reflected. The experiment done with live Korean mail piece images has demonstrated the superiority of the Proposed method. The success rate for 1, 988 testing images was about 93.56%.

• Atmosphere
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• v.29 no.4
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• pp.417-427
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• 2019

The purpose of this study is to build urban canopy model (Met Office Reading Urban Surface Exchange Scheme, MORUSES) based to Unified Model (UM) by using urban building information data in Seoul, and then to compare the improving urban canopy model simulation result with that of Seoul Automatic Weather Station (AWS) observation site data. UM-MORUSES is based on building information database in London, we performed a sensitivity experiment of UM-MOURSES model using urban building information database in Seoul. Geographic Information System (GIS) analysis of 1.5 km resolution Seoul building data is applied instead of London building information data. Frontal-area index and planar-area index of Seoul are used to calculate building height. The height of the highest building in Seoul is 40m, showing high in Yeoido-gu, Gangnam-gu and Jamsil-gu areas. The street aspect ratio is high in Gangnam-gu, and the repetition rate of buildings is lower in Eunpyeong-gu and Gangbuk-gu. UM-MORUSES model is improved to consider the building geometry parameter in Seoul. It is noticed that the Root Mean Square Error (RMSE) of wind speed is decreases from 0.8 to 0.6 m s^-1 by 25 number AWS in Seoul. The surface air temperature forecast tends to underestimate in pre-improvement model, while it is improved at night time by UM-MORUSES model. This study shows that the post-improvement UM-MORUSES model can provide detailed Seoul building information data and accurate surface air temperature and wind speed in urban region.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1337-1345
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• 2005

Vibrio vulnificus is the causative agent of foodborne diseases such as gastroenteritis and life-threatening septicemia. Microbial pathogenicity is a complex phenomenon in which expression of numerous virulence factors is frequently controlled by a common regulatory system. In the present study, a mutant exhibiting decreased cytotoxic activity toward intestinal epithelial cells was screened from a library of V. vulnificus mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, an open reading frame, fexA, a homologue of Escherichia coli areA, was identified and cloned. The nucleotide and deduced amino acid sequences of the fexA were analyzed, and the amino acid sequence of FexA from V. vulnificus was $84\%\;to\;97\%$ similar to those of AreA, an aerobic respiration control global regulator, from other Enterobacteriaceae. Functions of the FexA were assessed by the construction of an isogenic mutant, whose fexA gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of fexA resulted in a significant alteration in growth rate under aerobic as well as anaerobic conditions. When compared to the wild-type, the fexA mutant exhibited a substantial decrease in motility and cytotoxicity toward intestinal epithelial cell lines in vitro. Furthermore, the intraperitoneal $LD_{50}$ of the fexA mutant was approximately $10^{1}-10^{2}$ times higher than that of parental wild-type. Therefore, it appears that FexA is a novel global regulator controlling numerous genes and contributing to the pathogenesis as well as growth of V. vulnificus.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.78-78
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• 2003

Embryonic stem (ES) cells, derived from preimplantation embryo, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In contrast, terminally differentiated cells do not usually alter their nature but frequently die or transform if they are exposed to inappropriate external stimulations. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES cells (MB03) and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2O$$_2$. Approximately 1$\times$10$^4$ cells were plated in 96 well plate and serum starved for overnight. The conditioned cells were exposed to a various concentration of $H_2O$$_2$ fur 24 hrs and loaded with neutral red (50$\mu\textrm{g}$/ml) for 4 hrs, washed with PBS for 2 min three times, and entrapped dye was dissolved out using acetic ethanol. Cytotoxicity was determined by reading the amount of dye in the medium using microplate reader. equipped with 575 nm filter. Relative amount of the dye entrapped within MB03 or HeLa were not significantly different when cells were exposed up to 0.4 mM $H_2O$$_2$. However, this sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2O$$_2$, while it was approximately 54% in MB03 suggesting that this concentration of $H_2O$$_2$ is the defensive threshold for HeLa cells. The resistance to oxidative stimulation reversed, however, when cells were co-treated with BSO (L-buthionine- 〔S, R〕-sulfoximine) which chelates intracellular GSH. This result suggests that cellular GSH is the major defensive mechanism of human ES cells. Induction of enzymes involved in GSH metabolism and type of cell death is currently being studied.

• The Journal of the Korea Contents Association
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• v.17 no.4
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• pp.434-443
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• 2017

The study was carried out to identify the relationship of patients' self-rating and nurses' rating on health literacy of hospitalized patients. Data were collected using the Korean Functional Health Literacy Test (KFHLT) which was revised for hospitalized patients and Single Item Literacy Screener. A total of 178 subjects, which consisted of 89 hospitalized patients and 89 nurses taking care of them, were recruited from medical and surgical wards of E university hospital. Collected data were analyzed using SPSS 23.0 program. The results showed that the average health literacy score of the subjects was $11.45{\pm}4.22$ out of 17 and the average correct answer rate was 67.3%. Patient health literacy was significantly different by age, education, occupation and reading handouts. The patients' self-rating had significant correlation with health literacy measured by KFHLT while nurses' rating did not. There was no significant correlation between patients' self-rating and nurses' rating. The findings suggest that patients' self-rating might be available to assess patient health literacy in hospitals and a careful approach is required when nurses use a single-item rating.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1485-1489
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• 2007

Achaete-scute like 2 (ASCL2) gene encodes a member of the basic helix-loop-helix transcription factor which is essential for the maintenance of proliferating trophoblasts during placental development. ASCL2 gene preferentially expresses the maternal allele in the mouse. However, it escapes genomic imprinting in the human. In this study, the complete open reading frame consisting of 193 amino acids of ASCL2 gene was obtained. Sequence analysis indicated that a C-G mutation existed in the 3' region between Meishan and Large White pigs. The polymorphism was used to determine the monoallelic or biallelic expression with RT-PCR-RFLP in pigs of Large $White{\times}Meishan$ $F_1$ hybrids. Imprinting analysis indicated that the ASCL2 gene expression was biallelic in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). PCR-RFLP was used to detect the polymorphism in 270 pigs of the "$Large\;White{\times}Meishan$" $F_2$ resource population. The statistical results showed highly significant associations of the genotypes and fat meat percentage (FMP), lean meat percentage (LMP) and ratio of lean to fat (RLF) (p<0.01), and significant associations of the genotypes and loin eye area (LEA) and internal fat rate (IFR) (p<0.05).

• The Journal of the Korea Contents Association
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• v.7 no.2
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• pp.109-116
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• 2007

To reduce the effect of color distortions on reading colors, it is more desirable to statistically process as many pixels in the individual color region as possible. This process may require segmentation, which usually requires edge detection. However, edges in color codes can be disconnected due to various distortions such as dark current, color cross, zipper effect, shade and reflection, to name a few. Edge linking is also a difficult process. In this paper, k-means clustering was performed on the images where edge detectors failed segmentation. Experiments were conducted on 311 images taken in different environments with different cameras. The primary and secondary colors were randomly selected for each color code region. While segmentation rate by edge detectors was 89.4%, the proposed method increased it to 99.4%. Color recognition was performed based on hue, a*, and b* components, with the accuracy of 100% for the successfully segmented cases.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.3
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• pp.154-160
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• 1970

The present paper deals with the growth of yellow croaker by scale age reading. This study is based on material from 596 specimens caught by the Danish seine in the Yellow Sea during the period from June 1967 to April 1968. Ring marks of the scale were formed from April to July, corresponding to the spawning season of the fish reported by Bae (1960). Growth rate of each radius of the ring was approximately 0.73. The relationship between the total length and radius of scales, and the relationship between the body weight and total length are represented by the following equations respectively: L=61.350R+50.184 $$W=4.298L^{3.227}\times10^{-3}$$ Maximum total length calculated by the diagram of Walford's growth transformation, $$L_{n+1}=0.6866L_n+10.8730$$, was 346.9mm. Growth curve of the fish can be expressed by the following von Bertalanffy's equation : $$L_t=346.9(1-e^{-0.376(t+0.609)})$$