• The Journal of Korean Academic Society of Nursing Education
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• v.11 no.2
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• pp.214-221
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• 2005

Purpose: This study was to investigate the perception of the myopia in early school-aged children's mother and develope myopia preventive education program. Method: Data was collected from June 1th to 24th, 2003. The sample group(two elementary schools in A city)consisted of 105students mother with normal vision(naked vision 1.0 or more), 105 students mother with subnormal vision(0.7 or less). The data was analyzed using S.A.S. For the test of homogeneity and significance of variable, Fisher's Exact test and Student's t-test were used. Results: 1. There was significant difference in perception of being changed the children's eyesight between normal and myopia group(t=7.86, p=.0492). 2. High scores of cause of myopia were distance from TV or computer to viewer and time of watching TV or using computer in both groups. 3. There was significant difference in perception score of cause of myopia in the myopia group was 13.26, and 18.04 in the normal group(t=3.01, p=0.003). Especially in the unbalanced and regulated diet, regulated exercise, distance of eyes reading books and illumination, normal group had more perception score than myopia group. Conclusion: A myopia education program for children and parents should be planed and the effect be verified.

• Korean Journal of Child Studies
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• v.31 no.6
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• pp.107-123
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• 2010

The purpose of this study was to evaluate the short term effectiveness of a cognitive ability improvement service for children, which is one of the 'Investment activities for Local Community Services' conducted by the Ministry for Health and Welfare. Results indicate that the longer the period of using cognitive improvement services for children, the more positively significant influence there is on their language abilities in terms of comprehension, expression, and reading-writing. Furthermore, these influences are stronger in children of low-income families than in children from higher income families. Certainly, this type of service improves infants' language abilities regardless of the income level of their families.

• Journal of Internet Computing and Services
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• v.17 no.4
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• pp.135-143
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• 2016

The Internet is being utilized as a means of communication channel for science information. In this study, an experiment was conducted against college students, in which participants were assigned to one of the following four conditions: source status(professor, student) x social categorization(in-group, out-group). When the participants finished reading an online news article, perceived usefulness of and attitude toward the science technology were measured. The results show that the effect of source expertise on perceived usefulness was moderated by social categorization.

• Journal of Korean Elementary Science Education
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• v.36 no.4
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• pp.428-438
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• 2017

The purpose of this study is to analyze the visual attention of elementary school students according to the editorial design of the 2007 2009 revised elementary science textbook 'Structure and function of our body'. For this purpose, eye movements were collected while elementary school students were watching real textbooks wearing mobile eye tracker. The BeGaze 3.7 program of SMI company was used analyzing eye movements. Twenty-six elementary school students participated voluntarily in mobile tracking research. Elementary students learned the contents of textbook related to 'digestive organ' and 'respiratory organ' by using double reading learning strategy. The results of this study are as follows. First, as a result of pre- and post-knowledge tests, there was no statistically significant difference in learning effect between 2007 revised and 2009 revised textbook editing design. Second, elementary school students tended to give more visual attention to text than textbook illustrations. Third, the selective attention and persistent attention of elementary students showed a very strong positive correlation (.940), but the selective attention and self-control showed a strong positive correlation (.499). Fourth, students with high level of attention and low level showed high visual occupancy in text than in illustrations. Fifth, elementary school students preferred the 2009 revised science textbook to the 2007 revised.

• Journal of Photoscience
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• v.7 no.2
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• pp.53-58
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• 2000

We performed the cloning of a chalcone synthase (CHS) gene, the key enzyme in the anthocyanin biosynthesis, from the cDNA library constructed with grape suspension cells irradiated UV-B. The PCR fragment was used to cloning the CHS gene. One CHS cDNA clone containing an open reading frame and a partial stilbene synthase (STS)cDNA, the stilbene-type phytoalexin, were isolated. The CHS cDNA clone (VCHS) showed 87% sequence homology with VvCHS (V.vinifea) and 72.3% identity with VSTSY(V.vinifea). its amino acid sequences were longer than any other CHS genes as 454 residues. Two genes were weakly expressed in white light irradiated cells, but highly induced in UV-B irradiated condition during 32 hours. Interestingly, the STS was quickly and abundantly expressed from 2 hours when supplemented with jasmonic acid (JA) and the maximum expression was observed at 4 hours and then gradually decreased. But, the additional UV-B or white light quickly degraded the STS expression than only JA treated grape suspension cells. The CHS also was rapidly induced with JA and the synergistical effect was observed at the addigional light treatment of UV-B or white light. These results are indicated that CHS and STS have different response mechanisms against the environmental stresses.

• Journal of Korean Society of Steel Construction
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• v.9 no.2 s.31
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• pp.221-228
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• 1997

The main purpose of this paper is to determine the dynamic optimal shapes of simple beam-columns with the constant volume. The parabolic function is chosen as the variable equation for the depth of regular polygon cross-section. The ordinary differential equation including the effect of axial load is applied to calculate the natural frequencies. The Runge-Kutta and Regula-Falsi methods are used to integrate the differential equation and compute the frequencies, respectively. Then the dynamic optimal shape whose lowest natural frequency is highest is determined by reading the critical value of the frequency versus section ratio curve plotted by the frequency data. In the numerical examples, the simple beam-columns are analysed and the numerical results of this study are shown in tables and figures.

• Toxicological Research
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• v.17
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• pp.319-321
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• 2001

Taihu Lake is a major water source for part of Yangtze Delta, which is one of the most urbanized and economically prosperous areas in China. In last couple of decades, some parts of the lake were highly polluted due to eutrophication. This study analyzed dioxin-like potential and mutagenic potential in surface water samples from Taihu Lake. The samples were prepared by XAD-2 resin procedure. A batch of biological assays, including dioxin-like potential microassay with the rat hepatocyte cell line H411E, and Ames test was employed in the research. Results showed that jour water samples have high content of dioxin-like biological potential, the highest activity TEQ to 2,3,7,8-TCDD was 48 pg/ι in sample 1. The mutagenic effect with reading-frame shifting mechanism was confirmed in 3 of 4 samples. The effective sewage treatment facilities and reliable monitoring surveillance system are urgently needed for this area.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1327-1332
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• 2004

The hemA gene encoding 5-aminolevulinic acid synthase (ALAS) was cloned from Rhodobacter capsulatus, and its nucleotide sequence was determined. DNA sequencing data revealed one open reading frame coding for a protein with 401 amino acids that displayed high similarity to the amino acid sequences of other known ALASs. The hemA gene was then cloned and expressed under the control of constitutive promotor in E. coli. The recombinant E. coli strain was able to accumulate 5-aminolevulinic acid to 21 mM in the liquid medium supplemented with 45 mM glycine and 120 mM succinate. In addition, a marked effect of the pH of the culture medium on ALA production was observed, and the optimum pH for culture medium was determined to be 5.8-6.3.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.938-943
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• 2004

A gene responsible for the chlorothalonil-biotransformation was cloned from the chromosomal DNA of Ochrobactrum anthropi SH35B, an isolated bacterium strain from soil. We determined the nucleotide sequences and found an open reading frame for glutathione S-transferase (GST). The drug-hypersensitive Escherichia coli KAM3 cells transformed with a plasmid carrying the GST gene can grow in the presence of chlorothalonil. The GST of O. anthropi SH35B was expressed in E. coli and purified by affinity chromatography. The fungicide chlorothalonil was rapidly transformed by the purified GST in the presence of glutathione. No significant difference in the chlorothalonil-biotransformation effect was observed among the thiol compounds (cysteine, reduced glutathione, and $\beta$-mercaptoethanol). Thus, the result reported here is the first evidence on the chlorothalonil-biotransformation by conjugation with the cellular free thiol groups, especially glutathione, catalyzed by the bacterial GST.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1093-1098
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• 2004

An open reading frame encoding CadC, consisting of 526 amino acids, was identified from the upstream region of the Vibrio vulnificus cadBA operon. The deduced amino acid sequences of the cadC were 22 to 78% similar to those reported from other Enterobacteriaceae. Functions of cadC gene on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutant, whose cadC gene was inactivated by allelic exchanges. The results demonstrated that the gene product of cadC contributes to acid tolerance of V. vulnificus, and that its contribution is dependent on prior exposure of cells to moderately acidic pH. The cellular level of cadB and cadA transcripts decreased in the cadC mutant, indicating that CadC exerts its effect on acid tolerance of V. vulnificus by enhancing the expression of cadBA in a pH-dependent manner.