• Title/Summary/Keyword: re-induction

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Experimentally Provoked Double Axes Formation in Xenopus laevis Embryos (Xenopus Laevis Embryo에서 실험적으로 유도한 2중체축의 형성)

  • Chung, Hae-Moon;George M. Malacinski
    • The Korean Journal of Zoology
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    • v.27 no.2
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    • pp.93-102
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    • 1984
  • Xenopus laevis eggs were de-jellied and manually manipulated to remove their vitelline membranes. They were then positioned in a variety of orientations re. gravity. That is the future ventral side was located upwards (opposed gravity) or downwards (faced gravity). Development through the tailbud stage was observed and the frequency of double axes formation recorded. Orientation of the egg re. its natural polarity was not an important factor in generating double axes. Its physical structure (flattened re. spherical), however, appeared to be important in determining the frequency of twinning. These observations give insights into the mechsnism of polarity establishment in anuran eggs, and provide methods which should be useful for studies on primary embryonic induction.

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Evaluation of the Herbal Extract Mixture for the Effects of Hair-Regrowth Compared to 3% Minoxidil; Elongation of Anagen Period on C3H Mice (모발생장기 유도 C3H 생쥐에 있어서 미녹시딜과 생약추출 혼합 조성물의 모발 재성장 유도 효능)

  • 이계호;한선일;박길흥;권영이
    • YAKHAK HOEJI
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    • v.47 no.1
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    • pp.14-19
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    • 2003
  • The hair cycle consists of three phases, growth (anagen), involution (catagen) and quiescence (telogen) phases. In order to evaluate hair re-growth effect of herbal extracts mixture containing the 70% ethanol extracts of Polygoni Multiflori Radix, Mori Cortex Radicis, Gingko Biloba Folium and Pine bud, we have examined the induction of the anagen phase and/or elongation of the anagen period using C3H mice. Morphological examination was done by Hattori' and Ogawa's method. Enzyme activities of ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GT) and alkaline phosphatase (ALP) was detected by Bessey-Lovry-Brock's method. Enzyme activity as a biochemical marker of hair cycle was investigated in the third hair cycle period of C3H mice after depilation. 3% Minoxidil treated group and herbal extract mixture treated group were shown 3 days earlier initiation of anagen than control group. In cycling mouse skin, ${\gamma}$-GT activity is pronounced during anagen and greatly diminished during telogen. Herbal extract mixture has shown promising hair re-growth effect on hair follicular cycles of C3H mice.

Genomic Variations of Rice Regenerants from Tissue Culture Revealed by Whole Genome Re-Sequencing

  • Qin, Yang;Shin, Kong-Sik;Woo, Hee-Jong;Lim, Myung-Ho
    • Plant Breeding and Biotechnology
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    • v.6 no.4
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    • pp.426-433
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    • 2018
  • Plant tissue culture is a technique that has invariably been used for various purposes such as obtaining transgenic plants for crop improvement or functional analysis of genes. However, this process can be associated with a variety of genetic and epigenetic instabilities in regenerated plants, termed as somaclonal variation. In this study, we investigated mutation spectrum, chromosomal distributions of nucleotide substitution types of single-nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) by whole genome re-sequencing between Dongjin and Nipponbare along with regenerated plants of Dongjin from different induction periods. Results indicated that molecular spectrum of mutations in regenerated rice against Dongjin genome ranged from $9.14{\times}10^{-5}$ to $1.37{\times}10^{-4}$ during one- to three-month callus inductions, while natural mutation rate between Dongjin and Nipponbare genomes was $6.97{\times}10^{-4}$. Non-random chromosome distribution of SNP and InDel was observed in both regenerants and Dongjin genomes, with the highest densities on chromosome 11. The transition to transversion ratio was 2.25 in common SNPs of regenerants against Dongjin genome with the highest C/T transition frequency, which was similar to that of Dongjin against Nipponbare genome.

A Activation Function Selection of CNN for Inductive Motor Static Fault Diagnosis (유도전동기의 고정자 고장 진단을 위한 CNN의 활성화 함수 선정)

  • Kim, Kyoung-Min;Kim, Yong-Hyeon;Park, Guen-Ho;Lee, Buhm;Lee, Sang-Ro;Goh, Yeong-Jin
    • The Journal of the Korea institute of electronic communication sciences
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    • v.16 no.2
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    • pp.287-292
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    • 2021
  • In this paper, we propose an efficient CNN application method by analyzing the effect of activation function on the failure diagnosis of the inductive motor stator. Generally, the main purpose of the inductive motor stator failure diagnosis is to prevent the failure by rapidly diagnosing the minute turn short. In the application of activation function, experiments show that the Sigmoid function is 23.23% more useful in accuracy of diagnosis than the ReLu function, although it is shown that ReLu has superiority in overall fixer failure in utilizing the activation function.

Effect of Induction Heat Bending Process on the Corrosion Properties of 316 Stainless Steel Pipes for Nuclear Power Plant (원자력발전소용 316 스테인리스강 배관의 부식특성에 미치는 유도가열벤딩공정의 영향)

  • Shin, Mincheol;Kim, Young Sik;Kim, Kyungsu;Chang, Hyunyoung;Park, Heungbae;Sung, Giho
    • Corrosion Science and Technology
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    • v.13 no.3
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    • pp.87-94
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    • 2014
  • Recently, the application of bending products has been increased since the industries such as automobile, aerospace, ships, and plants greatly need the usage of pipes. For facility fabrication, bending process is one of key technologies for pipings. Induction heat bending process is composed of bending deformation by repeated local heat and cooling. Because of local heating and compressive strain, detrimental phases may be precipitated and microstructural change can be induced. This work focused on the effect of induction heat bending process on the properties of ASME SA312 TP316 stainless steel. Evaluation was done on the base metal and the bended areas before and after heat treatment. Microstructure analysis, intergranular corrosion test including Huey test, double loop electropotentiokinetic reactivation test, oxalic acid etch test, and anodic polarization test were performed. On the base of microstructural analysis, grain boundaries in bended extrados area were zagged by bending process, but there were no precipitates in grain and grain boundary and the intergranular corrosion rate was similar to that of base metal. However, pitting potentials of bended area were lower than that of the base metal and zagged boundaries was one of the pitting initiation sites. By re-annealing treatment, grain boundary was recovered and pitting potential was similar to that of the base metal.

The Effect of Priming Attribution of Chance versus Effort on Task Satisfaction and Re-performance Intention (우연 대 노력 귀인점화가 과제 만족과 재수행 의도에 미치는 효과)

  • Lee, Byung-Kwan;Lee, Guk-Hee
    • Science of Emotion and Sensibility
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    • v.16 no.4
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    • pp.535-544
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    • 2013
  • Prior research on attribution has paid much attention to cognitive processes through which people infer causes. However, surprising omission from this research stream is the role of unconscious priming of different causes in task satisfaction and re-performance intention. This research is conducted to examine how attributing to different causes (chance vs. effort) via priming procedure affects task satisfaction and re-performance intention. To do this, participants were assigned to either chance-priming or effort-priming attribution condition and asked to answer O/X quiz (Exp 1) or multiple choice questions (Exp 2). Then, we provided 'Good' or 'Bad' feedback to participants randomly regardless of their actual task results. Finally, we measured their task satisfaction and re-performance intention. Results indicate that task satisfaction is affected by the valence of feedback they received but re-performance intention is influenced by interaction between attribution priming and the valence of feedback. Specifically, when receiving 'Good' feedback, participants in effort-priming condition have higher re-performance intention than those in chance-priming condition, whereas when receiving 'Bad' feedback, participants in effort-priming condition have lower re-performance intention than those in chance-priming condition. Implications of the findings are discussed in relation to the research for induction and decision making as well as psychology of addiction.

Improving effect of Artemisiae Capillaris Herba extract in reflux esophagitis rats (인진호 추출물의 급성역류성 식도염 유발 흰쥐에 대한 개선 효과)

  • Lee, Joo Young;Seo, Bu Il;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.31 no.6
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    • pp.37-44
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    • 2016
  • Objective : This study aimed to evaluate the protective effect of Artemisiae Capillaris Herba (AC) in reflux esophagitis (RE) rats. Methods : The AC was measured antioxidant activity through in vitro experiments, such as total polyphenol and flavonoid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity. Base on the results, we had conducted in vivo experiments. Rats were divided normal, control, AC treatment 50 mg/kg BW (AC50), and AC treatment 100 mg/kg BW (AC100) groups. AC were orally administered 2 h before the induction of RE. RE was induced by tie the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats. The rats were sacrificed 5 h after the surgery. We analyzed the expression of inflammatory related markers by western blot and observed the production of reactive oxygen species (ROS) and hematoxylin-eosin staining, Results : The $IC_{50}$ of AC for DPPH and ABTS were showed 12.60 and $33.32{\mu}g/m{\ell}$ respectively. In the RE rat, AC decreased inflammatory related markers, such as phosphorylated inhibitor of ${\kappa}B{\alpha}$, nuclear factor-kappa B, cyclooxygenase-2, inducible nitric oxide synthase, and tumor necrosis factor alpha. Also, AC reduced the increased reactive oxygen species in serum. The anti-inflammatory effect of AC appeared to be partially mediated through the inhibition of ROS. Also, AC markedly ameliorated esophageal mucosa damage via the inhibition of protein expression related to inflammation. Conclusions : Therefore, these results suggest that AC would be used as a therapeutic material in protection and/or treatment for reflux esophagitis.

Root Colonization by Beneficial Pseudomonas spp. and Bioassay of Suppression of Fusarium Wilt of Radish (유용 Pseudomonas 종의 근면점유와 무우 Fusarium시들음병의 억제에 관한 생물학적 정량)

  • Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.25 no.1 s.80
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    • pp.10-19
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    • 1997
  • Fusarium wilt of radish (Raphanus sativus L.) is caused by the Fusarium oxysporum f. sp. raphani (FOR) which mainly attacks Raphanus spp. The pathogen is a soil-borne and forms chlamydospores in infected plant residues in soil. Infected pathogen colonizes the vascular tissue, leading to necrosis of the vascular tissue. Growth promoting beneficial organisms such as Pseudomonas fluorescens WCS374 (strain WCS374), P. putida RE10 (strain RE10) and Pseudomonas sp. EN415 (strain EN415) were used for microorganisms-mediated induction of systemic resistance in radish against Fusarium wilt. In this bioassy, the pathogens and bacteria were treated into soil separately or concurrently, and mixed the bacteria with the different level of combination. Significant suppression of the disease by bacterial treatments was generally observed in pot bioassy. The disease incidence of the control recorded 46.5% in the internal observation and 21.1% in the external observation, respectively. The disease incidence of P. putida RE10 recorded 12.2% in the internal observation and 7.8% in the external observation, respectively. However, the disease incidence of P. fluorescens WCS374 which was proved to be highly suppressive to Fusarium wilt indicated 45.6% in the internal observation and 27.8% in the external observation, respectively. The disease incidence of P. putida RE10 mixed with P. fluorescens WCS374 or Pseudomonas sp. EN415 was in the range of 10.0-22.1%. On the other hand, the disease incidence of P. putida RE10 mixed with Pseudomonas sp. EN415 was in the range of 7.8-20.2%. The colonization by FOR was observed in the range of $2.4-5.1{\times}10^3/g$ on the root surface and $0.7-1.3{\times}10^3/g$ in the soil, but the numbers were not statistically different. As compared with $3.8{\times}10^3/g$ root of the control, the colonization of infested ROR indicated $2.9{\times}10^3/g$ root in separate treatments of P. putida RE10, and less than $3.8{\times}10^3/g$ root of the control. Also, the colonization of FOR recorded $5.1{\times}10^3/g$ root in mixed treatments of 3 bacterial strains such as P. putida RE10, P. fluorescens WCS374 and Pseudomonas sp. EN415. The colonization of FOR in soil was less than that of FOR in root part. Based on soil or root part, the colonization of ROR didn't indicate a significant difference. The colonization of introduced 3 fluorescent pseudomonads was observed in the range of $2.3-4.0{\times}10^7/g$ in the root surface and $0.9-1.8{\times}10^7/g$ in soil, but the bacterial densities were significantly different. When growth promoting organisms were introduced into the soil, the population of Pseudomonas sp. in the root part treated with P. putida RE10 was similar in number to the control and recorded the low numerical value as compared with any other treatments. The population density of Pseudomonas sp. in the treatment of P. putida RE10 indicated significant differences in the root part, but didn't show significant differences in soil. The population densities of infested FOR and introduced bacteria on the root were high in contrast to those of soil. P. putida RE10 and Pseudomonas sp. EN415 used in this experiment appeared to induce the resistance of the host against Fusarium wilt.

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Detonation Wave Propagation Through a T-type Branch Tube in Combustion Wave Rocket Igniter (연소파 로켓 점화기의 T형 분기관내 데토네이션파 전파)

  • ;Vigor Yang
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2003.05a
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    • pp.221-224
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    • 2003
  • A numerical study is carried out for the detonation wave propagation through a T-branch. The T-branch is a crucial part of the combustion wave igniter, a novel concept of rocket ignition system aimed for the simultaneous ignition of multiple combustion chambers by delivering detonation waves. Euler equation and induction parameter equation are used as governing equations with a reaction term modeled from the chemical kinetics database obtained from a detailed chemistry mechanism. Second-order accurate implicit time integration and third-order space accurate TVD algorithm were used for solution of the coupled equations. Over two-million grid points enabled the capture of the dynamics of the detonation wave propagation including the degeneration and re-initiation phenomena, and some of the design factors were be obtained for the CWI flame tubes.

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