• Title/Summary/Keyword: rbcS

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Physiological Responses of Starry Flounder Platichthys stellatus during Freshwater Acclimation with Different Speeds in Salinity Change (염분변화 속도를 달리한 담수순화 과정에서 강도다리 Platichthys stellatus의 생리학적 반응)

  • Kim, Young-Soo;Do, Yong-Hyun;Min, Byung-Hwa;Lim, Han-Kyu;Lee, Bok-Kyu;Chang, Young-Jin
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.28-33
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    • 2009
  • Physiological responses (hematological factors, cortisol, glucose, osmolality, $Na^+$, $K^+$ and $Cl^-$) in starry flounder Platichthys stellatus were investigated during freshwater acclimation in the conditions of different speeds in salinity change with acute-decrease (AD) or stepwise-decrease (SD I and II). In AD of acute-decrease salinity, hematocrit (Ht), red blood cell (RBC) and hemoglobin (Hb) were rapidly increased more than SD I of stepwise-decrease salinity. But in case of SD II, Ht, RBC and Hb were no significant difference from beginning to end of this experiments. In AD, cortisol level significantly increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $13.7{\pm}0.2{\mu}g/mL$ at 6 hours and recovered to the basal levels ($3.1{\mu}g/mL$) at 10 days. In SD I, cortisol level was significantly increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $13.6{\pm}0.6{\mu}g/mL$ at 6 hours and recovered to the basal levels ($3.1{\pm}0.4{\mu}g/mL$) at 10 days. In SD II, cortisol level was a little increased from $2.1{\pm}1.0{\mu}g/mL$ at the beginning to $10.5{\pm}2.5$, $10.8{\pm}5.6{\mu}g/mL$ at 6, 12 hours and recovered to the basal level at 48 hours. Glucose level of AD, SD I, II were no significant difference from beginning to end of this experiments. Osmolality was $286.8{\pm}3.3\;mOsm/kg$ at the beginning. In SD II of stepwise-decrease, osmolality was no significant difference during rearing in freshwater (FW). But AD of stepwise-decrease and SD I of stepwise-decrease, osmolality was a little decreased end of this experiments. In AD of acute-decrease, only $Cl^-$ level was showed no significant difference from beginning to end of experiment and $Na^+$, $K^+$ levels were decreased. In case of SD I, $Cl^-$ level was showed no significant difference from beginning to end of experiment and $Na^+$, $K^+$ levels were decreased.

Effects of Ethanol and Saccharin on the Immune Response in Rats (랏트에 있어서 에탄올과 삭카린이 면역반응에 미치는 영향)

  • 안영근;김주영;김정훈;염정열
    • Environmental Analysis Health and Toxicology
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    • v.2 no.1_2
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    • pp.33-42
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    • 1987
  • Experiments were performed to investigate effects of ethanol and saccharin on the immune system in rats. 4% ethanol and 0.02, 0.20, 2.00% saccharin solution in 4% ethanol were provided ad libitum by tap water for 4 weeks. Rats were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by relative immuno organ weight, antibody production, Arthus reaction, delayed type hypersensitivity, and rosette forming cell. Ethanol exposure decreased thymus weight and delayed type hypersensitivity. A combined solution of ethanol and saccharin decreased water intake, growth rate, spleen weight, thymus weight, humoral and cellular immune response. Especially, a 2% saccharin solution in 4% ethanol very significantly suppressed cellular immunity.

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Effect of Ginseng petroleum Ether Fraction on the Immunotoxicity of Cadmium in Mice (I) (마우스에 있어서 카드뮴의 면역독성에 미치는 인삼석유 Ether 분획의 영향( I ))

  • 안영근;김정훈;이상근;황갑수
    • Environmental Analysis Health and Toxicology
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    • v.3 no.1_2
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    • pp.43-54
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    • 1988
  • Experiment was performed to investigate the immunotoxicity of cadmium administered orally and the effect of ginseng petroleum ether fraction on it. Mice were given 3, 30, or 300 ppm cadmium as cadmium chloride orally in the drinking water and injection of ginseng petroleum ether fraction intraperitoneally for 4 weeks. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Humoral immune response was evaluated by antibody production and Arthus reaction. Body weight gain, spleen weight, thymus weight, and liver weight were also measured. In the present study, cadmium generally suppressed the humoral immunity. There was decrease in the rate of body weight gain and liver weight to body weight by cadmium-administration. Ginseng petroleum ether fraction showed restoring effect, to some extent, on the decrease in PFC and in the rate of liver weight to body weight by cadmium-administration. But it more suppressed Arthus reaction.

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Blood and Serum Chemical Values on Deer Bred in Taegu Dalseung Park (사슴의 혈액 및 혈액화학치에 관한 연구)

  • 이근우;장인호;송재찬;이동석
    • Journal of Veterinary Clinics
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    • v.13 no.2
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    • pp.127-129
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    • 1996
  • The study was conducted to determine normal blood and serum chemical values on deer. Normal blood and serum chemical values were studied in 8 healthy deers bred at Dalseung Park in Taegu. The values were analyzed with a blood chemical analyzer and calculated by Chawen's statistical analysis. The blood values of RBC, WBC and PCV were $9.73{\pm}1.19{\times} 10^{6}/{\mu}l, 3.37{\pm}0.88{\times}10^{3}/{\mu}l, 47.25{\pm}4.65 % respectively. The serum chemical value were: GOT; 83.0{\pm}39.4 IU/L, GPT; 50.12{\pm}25.83 IU/L, BUN; 36.63{\pm}8.70 mg/dl. CPK ; 1,296{\pm}495 IU/L, ALP; 238{\pm}54 IU/L, glucose; 143{\pm}52 mg/dl, total protein; 6.95{\pm}0.61 g/dl, amylase ; 116.0{\pm}34.5 IU/L,$ respectively. These values will be clinically helpful to diagnose the disease of deer.

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Effect of Doses of Capsaicin on the Immune Response in Rats (Rat에 있어서 Capsaicin의 투여량이 면역반응에 미치는 영향)

  • 안영근;김주영;김정훈;노권태
    • Environmental Analysis Health and Toxicology
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    • v.4 no.1_2
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    • pp.19-26
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    • 1989
  • Experiments were performed on rats to investigate the effect of doses of capsaicin on the immune response. Olive oil and the 0.3 mg, 1.0 mg and 3.0 mg/kg administration of capsaicin in olive oil were injected intraperitoneally every day for 4 weeks. Rats were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by organ weight, HA and HY titer, Arthus reaction, delayed type hypersensitivity (DTH) and Rosette froming cell. Following results have observed. 1) The weight of spleen and thymus were increased by doses of compared with control group, but the body weight of rats was decreased. 2) HA titer, Arthus reaction and DTH were significntly decreased by doses of capsaicin as compared with control group. 3) Rosette forming cell in spleen cells was decreased according to the increase of capsaicin doses. These results suggested that high dose of capsaicin decrease humoral and cellular immune response in rats.

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Effect of Perilla Oil Diet on the Immune Response in Mice (마우스에 있어서 식이들기름이 면역반응에 미치는 영향)

  • 안영근;김정훈;김도훈
    • Environmental Analysis Health and Toxicology
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    • v.3 no.3_4
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    • pp.17-28
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    • 1988
  • The effects of perilla oil diet on the immune response in mice have been studied. ICR male mice were divided into 4 groups and were fed on the experimental diet for 4 weeks. Mice were sensitized and challenged with sheep red blood cell (S-RBC). Immune response were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette forming cell (RFC) and macrophage activity. The weight of body, liver, thymus and spleen were measured. The body weight was increased but thymus weight was not altered by them. The perilla oil diet decreased the weight of liver and spleen in mice. It reduced antibody production, Arthus reaction, DTH and RFC, macrophage activity. These results showed that the high perilla oil diet decresed more humoral and celluar immune response than the low perilla oil diet. It decreased the phagocytic activity on the reticuloendothelial system in mice.

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Effects of Kimchi Consumption on Iron Status in Adult Male Volunteers (김치의 섭취가 성인 남성의 철분영양상태 지표에 미치는 영향)

  • 오영주
    • Journal of Nutrition and Health
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    • v.30 no.10
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    • pp.1188-1194
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    • 1997
  • The aim of this was to investigate whether the regular consumption of kimchi influences the iron status (RBC , Hb, Ht, MCH, MCV, MCHC, transferrin , serum iron, and ferritin) in volunteers. Healthy male adults(n=12) took part in the study subdivided into the control Ⅰ-phase(for 2weeks), kimchi-phase (for 4 weeks), and control Ⅱ-phase(for 2 weeks). In addition to their normal diet, participant consumed 300g of lactic acid fermented Chinese cabbage kimchi daily for four weeks. In the control Ⅰ and control Ⅱ phases, the participants kept up their normal diets without consuming any fermented foods. Dietary intakes were recorded for 3 consecutive days in each phase, with the aid of household measures. Every two weeks. blood specimens were analysed. Significant differences(p<0.05) between the phases were found in MCHC, and transferrin in blood were not significantly changed during kimchi consumption. However, serum iron and ferritin levels were significantly increased(p<0.05) during kimchi consumption, achieving the highest levels in the fourth week of the kimchi components(ascrobic acid, sulfer compound, organic acid, capsaicin, gingerol , allicin). Because of lacticacid fermented kimchi's potential to prevent anemia , the consumption of this food can be recommended.

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Preliminary search of intraspecific chloroplast DNA variation of nine evergreen broad leaved plants in East Asia

  • Lee, Jung-Hyun;Lee, Byoung-Yoon;Choi, Byoung-Hee
    • Korean Journal of Plant Taxonomy
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    • v.41 no.3
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    • pp.194-201
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    • 2011
  • In order to acquire information on chloroplast DNA markers to evaluate the genetic diversity of evergreen broad leaved plants, we investigated the intraspecific variation of cpDNA in eight non-coding regions of nine species commonly distributed in East Asia. Although no variations were detected in psbA-trnH, rpoB-trnC, rpl16 and atpB-rbcL regions, a relatively large amount of intraspecific variations was detected in the psbC-trnS, rps16 and trnL-F regions. These results suggested that these three cpDNA markers are suitable to assess genetic diversity of the species investigated in this study. In contrast, intraspecific variations were detected in seven taxa except Hedera rhombea and Neolitsea aciculata. Neolitsea sericea and the taxa of Quercus had many polymorphic sites.

SOC Sustaining Strategy for HEV through State-machine Control (하이브리드 차량의 SOC 유지전략 방법)

  • Byun, Sang-Min;Kim, Beom-Soo;Cha, Suk-Won
    • New & Renewable Energy
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    • v.4 no.4
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    • pp.65-71
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    • 2008
  • Considering the world's environmental problem, HEVs are projected as one of the solution. The keys of the HEV cruise control are expanding the use of electric motor and operating the internal combustion engine in the efficient region. This paper presents a new structure of SOC sustaining model where state-machine control is used. The proposed model defines battery charging and discharging as states and SOC of the battery as control variables. In this paper, we introduce various methods in deterministic rule-based control for HEV and describe a new SOC sustaining controller used by state-machine.

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Babesia gibsoni Infection in Three Hunting Dogs (사냥개에서의 Babesia gibsoni 감염)

  • Shin Sang-Tae;Choi Hee-In;Sung Jai-Ki;Lee Chang-Woo
    • Journal of Veterinary Clinics
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    • v.4 no.2
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    • pp.505-514
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    • 1987
  • Babesia gibsoni infection was diagnosed and treated in three hunting dogs which were hospitalized to the Veterinary Teaching Hospital, College of Veterinary Medicine, Seoul National University between April 4 and August 29, 1987. All three dogs revealed severe anemia, hemoglobinuria, splenomegaly and markedly decreased PCV, RBC count and hemoglobin. The anemia was regenerative, as characterized by increased numbers of nucleated erythrocytes, polychromasia, anisocytosis, reticulocytosis. B. gibsoni was identified by examination of blood smear stained with Giemsa stain. The forms of B. gibsoni identified in this report were pleomorphic such as singnet ring, oval, comma, dot and elongated forms. The maximal percentages of erythrocytes infected with one or more B. gibsoni organisms were 39%, 20% and 40%, respectively. The Tick, Haemophysalis longicornis was assumed to be the vector of babesiosis in these cases. Specific treatment consisted of diminazene aceturate and supportive treatment consisted of whole blood transfusion, lactated Ringer's solution, vitamin B complex and broad spectrum antibiotics. All three dogs had convalesced successfully after treatment.

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