• The Korea Journal of Herbology
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• v.21 no.4
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• pp.59-67
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• 2006

Objectives : Sam-Myo-Whan(SMW) has been known traditional prescription with anti- anthritis activities. We investigated inhibitory effects of SMW on lipopolysaccharide (LPS)-induced nitric oxide(NO), $TNF-{\alpha}$ and inducible nitric oxide synthase(iNOS) production from RAW264.7 cells and BV-2 Microglia cells. Methods : SMW, which had been extracted with 70% MeOH, concentrated and freeze-dried was used for this experiment. After BV2 mouse brain macrophages and RAW264.7 mouse peritoneal macrophages were pretreated with increasing concentrations of SMW extract for 30min, and then activated with LPS. To investigate cytotoxicity of SMW extract, cell viability was measured by MTT assay. NO production was measured in each culture supernatant by Griess reaction. mRNA expression of iNOS in two type cells was investigated by RT-PCR. $TNF-{\alpha}$ production was measured in each culture supernatant by ELISA. Results : SMW extract significantly inhibited LPS-induced NO and $TNF-{\alpha}$ production in BV2 cells and RAW264.7 cells dose-dependently. SMW extract also greatly suppressed mRNA expression of iNOS in both type cells activated with LPS. Conclusion : These data suggests that SMW extract may have an anti-inflammatory effect through the inhibition of iNOS expression.

• The Korea Journal of Herbology
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• v.30 no.6
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• pp.33-38
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• 2015

Objectives : Portulaca oleracea (PO) have been used as a traditional medicine to treat inflammatory diseases in Korea. However, the anti-inflammatory effect of PO ethanol extract on lipopolysaccharide (LPS)-induced inflammation is not well-known. Therefore, this study was performed to identify the anti-inflammatory effect of PO on LPS induced inflammatory.Methods : Identification of PO was conducted by comparison with purified standards by HPLC. To measure out the cytotoxicity of PO, author performed the MTT assay. To evaluate the anti-inflammatory effects of PO, author examined the inflammatory mediators such as nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin, (IL)-1β and IL-6) on RAW 264.7 cells. Author also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor-B (NF-κB) activation by western blot.Results : Three major components (peaks 1, 2, 3) were detected in both varieties and peak 1 was characterized as caffeic acid, peak 2 as p-coumaric acid, and peak 3 as ferulic acid by comparison of chromatographic properties with authentic standards. Extract from PO itself did not have any cytotoxic effect in RAW 264.7 cells. PO inhibited LPS-induced productions of inflammatory mediators such as NO and pro-inflammatory cytokines in RAW 264.7cells. In addition, PO inhibited the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2), c-Jun NH₂-terminal kinase (JNK) and NF-κB activation in RAW 264.7 cells.Conclusions : Above experiment data can be an important indicator for the identification of PO and this study suggest that treatment of PO could reduce the LPS-induced inflammation. Thereby, PO could be used as a protective agent against inflammation.

• BMB Reports
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• v.42 no.9
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• pp.574-579
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• 2009

The regulatory effects of honokiol on the cellular responses of macrophages and monocytes were evaluated. Specifically, we investigated the effects of honokiol with respect to lipopolysaccharide (LPS)-induced cytotoxicity, LPS- or phorbol-12-myristate-13-acetate (PMA)-mediated morphological changes, and relevant events (FITC-dextran-induced phagocytic uptake). Honokiol blocked the LPS-induced cytotoxicity of RAW264.7 cells in a dose-dependent manner. In addition, honokiol appeared to block the production of cytotoxic cytokines such as interleukin (IL)-$1{\beta}$ and tumor necrosis factor (TNF)-$\alpha$, nitric oxide (NO), and reactive oxygen species (ROS). Moreover, honokiol strongly prevented the morphological changes in RAW 264.7 and U937 cells that were induced by LPS and PMA. The surface levels of marker proteins, which are up-regulated under the morphological changes of RAW264.7 and U937 cells, were also diminished. The data presented here strongly suggest that the honokiol modulates various cellular responses managed by macrophages and monocytes.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 1994.05a
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• pp.37-44
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• 1994

The purpose of this research is to present necessity of manament system establishment and policy direction for fundmental data, understanding management reality and problem, comparing resident's management attitude to related variables in apartment and raw houses multi-family houses. The major finding were as follows : 1) The management attitude of Apartment inhabitant is very highm especially the attitude of maintenance management is the higest. But they disagree with the raise of management cost necessary to maintenance management. 2) The management attitude of Raw multi-family houses inhbitant is especially high, the maintenance management demand for safety and community-life contol attitude, resident's mutual cooperation is especially high, they think much of autonomy management, desire to achieve under more sysemnatic plan.

• The Korean Journal of Food And Nutrition
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• v.1 no.1
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• pp.7-12
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• 1988

In the view of the fact that data are scare concerning the change of Methionine composition of poultry meat in function of storage temperature and cooking method. The present study was carried out to change in these compounds and the results were summarized as follows. 1. The result of measuring methionine compound of raw material stored under the condition of 5$^{\circ}C$, 1$0^{\circ}C$ for 6 weeks were increased as temperature increases. 2. The result of change in methionine compound of roasting were decreased than raw material It was greatly decreased in the storage for 42 days at 1$0^{\circ}C$ and slightly decreased in the storage for 14 days at 5$^{\circ}C$ 3. When the boiling in the water, methionine compound was decreased than roasting to compare with raw material 4. By the results of this study, one of the best cooking method is roasting, because of the change in methionine compound of roasting is slightly decrease.

• Journal of the Korean Society of Safety
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• v.33 no.2
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• pp.39-44
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• 2018

Dust explosions are occurring in a variety of industries. A dust explosion caused by a specific energy generates huge amount of energy in the ignition and releases decomposition gas. Damages can be increased since this released decomposition gas can cause second and subsequent explosions. In this study, the goal was to obtain practical information on what could affect the explosion by comparing the characteristics of two kinds of dusts with completely different chemical properties. Three kinds of dusts were measured and evaluated for explosion pressure, dust explosion index, explosion limit and minimum ignition energy. It is possible to grasp the characteristics of each dust and use it as useful accident prevention data in the production of raw material powder.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.187.1-187.1
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• 2011

RDF(Refuse-Derived Fuel) is a fuel of pelletized form made of combustible solid wastes and can not only be used as alternative energy to fossil fuel but also solve troubles in thermal uses of incinerator. As the first stage for obtaining elementary data required to develop criteria of raw materials appropriate to RDF combustion facilities actively spread recently in Korea, preliminary experiments were conducted on CO, SOx, NOx and HCl production and reduction characteristics in combustion of RDF. RDF samples weighing 2~3 g per a sample were manufactured in a lab-scale way and combustion tests of RDF were carried out in electric furnace with quartz tube of 50 mm inside diameter.

• Journal of the Korean Home Economics Association
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• v.19 no.1
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• pp.47-52
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• 1981

In the view of the fact that data are scare concerning the change of Methionine composition of poultry meat in function of storage temperature and cooking method. The present study was carried out to change in these compounds and the results were summarized as follows. 1. The result of measuring Methionine compound of Raw material stored under the condition of $5^{\circ}C$, $10^{\circ}C$ for 6 weeks were increased as temperature increases. 2. The result of change in Methionine compound of Roasting were deceased than Raw material It was greatly decreased in the storage for 42 days at 1$0^{\circ}C$ and slightly decreased in the storage for 14 days at $5^{\circ}C$. 3. when the Boiling in the water, Methoinine compound was decreased than Roasting to compare with Raw material 4. By the results of this study, one of the best cooking method is Roasting, Because of the change I Methionine compound of Roasting is slightly decrease.

• Journal of Dairy Science and Biotechnology
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• v.30 no.2
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• pp.69-74
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• 2012

This study was performed to determine by the ability of the mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus to ferment Dioscorea opposita Thunb. (yam) and to evaluate the nutraceutical value of fermented yam. The titratable acidity (TA) value increased from 2 to 6% with increased concentrations in both raw yam and extracted lactic acid bacteria (LAB) fermented yam (LFY). The viable cell counts and the allantoin and diosgenin contents were higher in raw LFY at large concentrations (6%) than in extracted LFY samples at all fermentation periods up to 32 h. Based on these data, it confirmed that raw yam fermented by the combination culture of L. bulgaricus and S. thermophilus for various fermentation periods favors the symbiotic growth of LAB and results in higher nutraceutical content.

• KSBB Journal
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• v.9 no.2
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• pp.108-114
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• 1994

Kinetic equations for transglycosylation of stevioside in the attrition coupled reaction system using raw starch as a glycosyl donor were derived considering that the reaction was carried out through two steps; production of cyclodextrin(CD) from raw starch in the attrition coupled reaction system and then transglycosylation of glycosyl residues to stevioside from produced CD. Kinetic constants of derived equation were evaluated. The simulation result showed that the derived kinetic equations could predict the experimental data reasonably well and that can be utilized for optimization and scale-up of transglycosylation reactor and process developments.