• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1760-1772
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• 2006

Mature acetylcholinesterase (AChE) gene (gm, 1,836 bp) was cloned from the housefly and successfully expressed in the E. coli CodonPlus (DE3) RIL system (GM-E, 72 kDa) with a yield of 1,630 mU/g fresh cells. Using the gm, 10 kinds of mutants were constructed and expressed for enhancing sensitivity to insecticides. The sensitivity of these mutants to five kinds of organophosphate (OP) and three carbamate insecticides was investigated by measuring the apparent bimolecular inhibition constant ($k_i=k_2/K_d$). Surprisingly, the sensitivity of quadruple mutant IGFT was enhanced as much as 7-fold for acephate, 164-fold for demeton-S-methyl, 484-fold for dichlorvos, 523-fold for edifenphos, 30-fold for ethoprophos, 30-fold for benfuracarb, 404-fold for carbaryl, and 107-fold for furathiocarb, compared with that of GM-E, although the sensitivity of each single point mutant was slightly increased. These mutational studies indicated that (i) contradictory to Walsh et al. [39], the residue 327 is the important key residue for enhancing sensitivity as much as the residue 262, (ii) the residue 82 and additional residues of 234, 236, and 585 are also important, and (iii) sensitivity was cooperatively accelerated as the number of strategic mutations increased.

• 한국항행학회논문지
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• 제9권2호
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• pp.109-120
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• 2005

본 논문에서는 MSSI 보상 기법을 채택한 1,200 km의 WDM 전송 시스템에서의 비트 에러율, 수신 감도, 입사 전력 최소 허용치를 증폭기 간격에 따라 수치적으로 살펴보았다. 변조 파형 형식에 관계없이 수신 감도와 입사 전력 최소 허용치 모두 증폭기 간격이 증가할수록 나빠지는 것을 알 수 있었다. 수신 감도 측면에서는 NRZ 전송보다 RZ 전송을 하는 경우 감도를 개선할 수 있었지만 실제 입사 전력 허용치 면에서는 NRZ가 RZ 전송보다 유리한 것을 확인하였다. 아울러 증폭기 간격을 50 km 이하로 해야만 수신 감도와 입사 전력 최소 허용치 모두 분산 계수, 채널 파장, 펌프광 전력에 비교적 영향을 적게 받는 것을 확인할 수 있었다.

• 약학회지
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• 제43권5호
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• pp.606-613
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• 1999

To survey the possibility of replacing the pyrogen test with Limulus Amebocyte Lysate(LAL) test and to find out a standard methods suitable to our blood products made in Korea, 100 samples of 20% human serum albumin were tested by commercial LAL test kits and results of those were compared with rabbit pyrogen test. The LAL test is used both dinetic-chromogenically and kinetic-turbidimetrically. Both methods equally showed broad detection range (5.0~0.005 EU/ml), excellent sensitivity ($\geq$ 0.005 EU/ml) and predominant recovery rate within valid dilution range, but kinetic-turbidimetric method seemed to be more reproducible than kinetic-chromogenic method(kinetic-chromogenic method : S.D. = 15.88, kinetic-turbidimetric method : S.D. = 8.12). After heating the sample at 75$^{\circ}C$ for 15 min, the results showed a little elevated recovery rate with both methods. After performing the test on 100 albumin samples with both kits, the results were analysed using the USP standard (1.33 EU/ml). 7% of samples in kinetic-chromogenic methods and 1% of samples in kinetic-turbidimetric method exceeded the limit of endotoxin levels regulated for blood products in USA. Because this phenomenon was not observed in both methods at the same time and both methods have high sensitivity ($\geq$0.005 EU/ml), these results seemed to depend on nonspecific reaction. Considering its sensitivity and reproducibility, we could assure that LAL test is proper to detecting pyrogenic with good sensitivity.

• International Journal of Automotive Technology
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• 제7권4호
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• pp.485-492
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• 2006

The AA5182/polypropylene/AA5182(AA/PP/AA) sandwich sheets have been developed for application to automotive body panels in future lightweight vehicles with significant weight reduction. It has been reported that the AA5182 aluminum sheet shows $L\"{u}ders$ band because of dissolved Mg atoms that cause fabrication process problem, especially surface roughness. The examination of serration behavior has been made after the tensile deformation of the AA/PP/AA sandwich sheets as well as that of the AA5182 aluminum skins at room and elevated temperatures. All sandwich sheets and the AA5182 aluminum skin showed serration behavior on their flow curves. However, the magnitude of serration was significantly diminished in the sandwich sheet with high volume fraction of the polypropylene core. According to the results of the analysis of the surface roughness following the tensile test, $L\"{u}ders$ band depth of the sandwich sheet evidently showed lower than that of the AA5182 aluminum skin. The strain rate sensitivity, m-value, of the AA5182 aluminum skin was -0.006. By attaching these skins to the polypropylene core, which has relatively large positive value of 0.050, m-value of the sandwich sheets changed to the positive value. The serration mechanism of the sandwich sheets was quantitatively investigated in the point of the effect on polypropylene thickness variation, that on the strain rate sensitivity and that on the localized stress state.

• Journal of Mechanical Science and Technology
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• 제17권1호
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• pp.57-63
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• 2003

An eigenvalue design sensitivity formulation of a general nonsymmetric-matrix rotor-bearing system is devised. using the DDM (direct differential method). Then, investigations on the design sensitivities of critical speeds are carried out for an APU turbogenerator with a spline shaft connection. Results show that the dependence of the rate of change of the critical speed on the stiffness changes of bearing models of spline shaft connection points is negligible, and thereby their modeling uncertainty does not present any problem. And the passing critical speeds up to the 4th critical speed are not sensitive to the design stiffness coefficients of four main bearings. Further, the dependence of the rate of change of the critical speed on the shaft-element length changes shows quantitatively that the spline shaft has some limited influence on the 4th critical speed but no influence on the 1st to 3rd critical speeds. With no adverse effect from the spline shaft, the APU system achieves a critical speed separation margin of more than 40% at a rated speed of 60,000 rpm.

• 동력기계공학회지
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• 제21권2호
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• pp.20-26
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• 2017

This study was carried out to investigate the effect of precipitate on the high temperature deformation stability of incoloy 825 alloy. $Cr_{23}C_6$ carbide was precipitated under $950^{\circ}C$, but was not detected over $1,000^{\circ}C$. Most of the precipitation consist of $Cr_{23}C_6$ carbide. Strain-rate sensitivity was the highest in 0.01/s and the lowest in 10/s. Strain-rate sensitivity was decreased sharply below $950^{\circ}C$. In the temperature between $850^{\circ}C{\sim}1,150^{\circ}C$, plastic instable area did not exist. It showed the lowest Ziegler Parameter value of 0.06 Ziegler Parameter was the lowest as 0.06 at $850^{\circ}C$ with 10s-1 of strain. The highest Ziegler Parameter value(0.43) was found in plastic deformation at $1,050^{\circ}C$ with 0.01s-1 of strain. It tends to have an higher resistance to the high temperature deformation under $950^{\circ}C$, due to the precipitation.

• 한국마린엔지니어링학회:학술대회논문집
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• 한국마린엔지니어링학회 2001년도 추계학술대회 논문집(Proceeding of the KOSME 2001 Autumn Annual Meeting)
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• pp.92-97
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• 2001

This paper was studied on the effect of temperature on corrosion of absorption refrigeration systems using LiBr-$H_2O$ working fluids. In the fresh water and 62 %lithium bromide solution at $70^{\circ}C$, polarization test of SS 400, Cu(Cl220T-OL) and Ni-Al bronze was carried out. And Polarization behavior, polarization resistance characteristics, corrosion rate(mmpy) and corrosion sensitivity of materials forming absorption refrigeration systems was considered. The main results are as following: As the experimental temperature increase, the change of corrosion rate of Ni-Al bronze become duller than SS 400 and Cu in 62 % lithium bromide solution. Open circuit potential of SS 400 is less noble than that of Cu and Ni-Al bronze in fresh water, but that becomes noble than Cu and Ni-Al bronze in 62 % lithium bromide solution. The corrosion sensitivity of Ni-Al bronze was controlled than that of Cu and SS 400 in 62% LiBr solution.

• 한국신뢰성학회지:신뢰성응용연구
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• 제17권2호
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• pp.92-102
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• 2017

Purpose: Reliability prediction standards consider environmental conditions, such as temperature, humidity and vibration in order to predict the reliability of the electronics components. There are many types of standards, and each standard has a different failure rate prediction model, and requires different environmental conditions. The purpose of this study is to make a sensitivity analysis by changing the temperature which is one of the environmental conditions. By observing the relation between the temperature and the failure rate, we perform the sensitivity analysis for standards including MIL-HDBK-217F, RiAC-HDBK-217Plus and FIDES. Methods: we establish environmental conditions in accordance with maneuver weapon systems's OMS/MP and mission scenarios then predict the reliability using MIL-HDBK-217F, RiAC-HDBK-217Plus and FIDES through the case of DC-DC Converter. Conclusion: Reliability prediction standards show different sensitivities of their failure rates with respect to the changing temperatures.

• Journal of Electrical Engineering and Technology
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• 제4권1호
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• pp.43-48
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• 2009

The purpose of the this paper is to make decision of the maintenance priority of power distribution system using Time-Varying Failure Rate(TVFR) with interruption cost. This paper emphasizes the practical use of the reliability indices and interruption cost. To make a decision of maintenance priority on power distribution system equipment, the quantification of the reliability level should be represented as a cost. In this paper, the TVFR of power distribution system equipment applied in this paper utilizes analytic method to use the historical data of KEPCO. From this result, the sensitivity analysis on TVFR of equipment was done for the priority, which represents that high priority of the equipment has more effect on system reliability, such as SAIDI or SAIFI, than other equipment. By this priority, the investment plan is established. In this result, customer interruption cost(CIC) could be extracted, and CIC is used as weighting factor to consider a importance of customer. After that, the result calculated the proposal method in this paper is compared with other priority method, such as lifetime, failure rate or only sensitivity.

• Nuclear Engineering and Technology
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• 제42권4호
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• pp.394-413
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• 2010

The object of this paper is to identify the key elements that impact a radiation dose at EAB (Exclusion Area Boundary). This study is based on the AST (Alternative Source Terms) as defined in Regulatory Guide 1.183. The LOCA (Loss of Coolant Accident) and the LRA (Locked Rotor Accident) are selected as limiting cases. A sensitivity analysis of accidental behavior with respect to various parameters during LOCA and LRA at Kori Unit 1 is also undertaken for the following objectives: to determine the limiting parameters, to find the impact trend of the radiation dose, and to find the safety margin between AST and TID (Technical Information Document) methodologies. This work confirms that key parameters are particulate removal rate, decontamination factor, iodine chemical form, gap fraction, partitioning factor, and the impact of isotopes group. Comparing TID with AST, the radiation dose of TID is about 80% greater than that of AST under a LOCA, and about 60% greater than that of AST for the case of a LRA; thus the safety margin is remarkably increased when the AST is used. In this work, the sensitivity analysis results are presented in terms of a sensitivity index called the "NDD (Normalized Dose Difference)", which compares the impact of parameters with that of a reference case. These values are derived by using a combination of the leak rate (primary to secondary), iodine chemical form, gap fraction, partitioning factor, spray removal rate, source term, and other variables.