• 사상체질의학회지
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• 제17권3호
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• pp.91-102
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• 2005

1. Objectives The purpose of this study is to find out effects of Hyeongbangpaedok-san(HBPDS) and Dokhwaljihwang(DHJH) against decline of physical function as aging. 2. Methods Administrating HBPDS and DHJH to 40-week-old Wistar rat for 10 weeks so, I researched weight change, weight change of internal organs, and hematological and serological changes. 3. Results & Conclusions 1. Both examining groups, which were taken HBPDS and DHJH, got more weight than control group. But that was regardless. 2. Both examining groups got more weight on internal organs than control group. But that was regardless, too. 3. Both examining groups decreased in amount of MDA in serum, as contrasted with control group. But it was regardless. 4. Both examining groups improved on hematological condition. WBC, RBC, Hgb, monocytes and eosinophil rates were decreasing and HCT and PLT were increasing. Especially monocytes(p<0.001) and eosinophil(p<0.05) rate of DHJH taken group was decreased remarkably. 5-1. Both examining groups show decline in each item of functional examination of liver, such as ALT, AST, T-bilirubin, T-protein, ALB, A/G. T-chol, TG, etc. HBPDS taken group showed meaningful decline in Albumin(p<0.01) and A/G(p<0.01) and DHJH taken group showed meaningful decline in T-bilirubin(p<0.01). 5-2. Both examining groups showed decline in items of functional examination of kidneys. Specially HBPDS taken group showed meaningful decline in CRN rate(p<0.05) and DHJH taken group showed meaningful decline in BUN rate(p<0.05). As those results, HBPDS and DHJH are effective against decline of physical function as aging.

• Molecular & Cellular Toxicology
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• 제3권4호
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• pp.299-305
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• 2007

The endocrine disrupting chemical, di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in polyvinyl chloride products ubiquitous in our daily lives. DEHP has potentially adverse effects on the liver, kidney, lung, heart, reproductive organs and endocrine systems. Many toxicological data on the DEHP toxicity have been stated, but complete protein profiles have not yet been reported. In this study, DEHP-induced oxidative DNA damage in rat lymphocyte was evaluated by Comet assay (single-cell gel electrophoresis) for the first time. Moreover, DEHP-induced protein profile alterations were examined in rat liver by using toxicoproteomic tools. 34 protein spots in the liver were identified to be significantly deregulated by DEHP on the 2-dimensional gel. Among them, 20 spots were up-regulated and 14 spots down-regulated by DEHP.

• BMB Reports
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• 제34권2호
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• pp.156-165
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• 2001

Leptin is a circulating non-glycosylated protein that is mainly produced in adipocytes. Leptin acts in the brain to regulate food intake and energy expenditure. Previously we reported our success in the isolation of a partial cDNA of the long form of the leptin receptor, OB-Rb, from rat spleen, and showed that leptin might also play a role in peripheral immune organs. In the present study, for the first time, the complete coding region of OB-Rb cDNA was cloned from rat splenocytes, and its nucleotide sequence was determined. The cDNA was then further expressed in E. coli and mammalian cells, thereby confirming the functional integrity of this receptor. Prokaryotically overexpressed OB-R protein was then used as an immunizing antigen in BALE/c mice to produce leptin receptor-specific antibodies. By using them, we confirmed the cell surface expression of OB-Rb in transfected CHO cells. It is our belief that the reagents, as produced in this study, will be of great use in further studies of the biological role of rat leptin.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.277-287
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• 2002

The chemopreventive effects of ginseng on rat carcinogenesis models were investigated, In the present study, the inhibitory effects of white and red ginseng on tumor development were examined using medium-term liver, initiation and medium-term multi-organ carcinogenicity bioassay systems. No modifying potential of the ginsengs was evident in terms of the numbers or areas of glutathione S-transferase placental form (GST -P)-positive foci, which is a marker of preneoplastic lesion in rat livers. However, white ginseng, but not red ginseng was found to decrease the incidences of adenocarcinoma of the small intestine and colon in the medium-term multi-organ carcinogenesis model. These results indicate that white ginseng may have inhibitory effects on progression stage of rat intestinal carcinogenesis, but the influence is not strong. Ginseng is unlikely to have promoting or inhibitory effects in other organs under the present type of experimental conditions. Possible application on ginseng for chemoprevention of colon cancer in humans, can be concluded given the lack of obvious adverse effects.

• 약학회지
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• 제33권5호
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• pp.300-307
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• 1989

Expression of transferrin gene in various organs of rat was studied using rat transferrin cDNA. The hybridization method of $[^{35}S]-labeled$ transferrin cDNA with transferrin mRNA in cytoplasmic preparations was used to measure the level of transferrin mRNA. The rat from 15-day old fetus to 21-day old postnatal were employed as an animal model. In the liver, the level of transferrin mRNA increased with increasing age. However, the level of transferrin mRNA in brain was significantly lower than that in liver and the level did not increase with age.

• 한국환경보건학회지
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• 제20권3호
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• pp.85-95
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• 1994

This study was performed to find out the detoxication effects of natural products Thuja orientalis, leaf of acorn, aloe ferox mill, pine cone, pine root, lonicerae flos on nickel toxicity. The experimental rats were divided into 3 groups such as nickel alone treatment group, natural products treatment groups before and after nickel treatments. Each group was administered with difference dose of nickel such as 6.6 mg/kg/day, 13.2 mg/kg/day, 19.8 mg/kg/day respectively, for 14 days. Natural products were administered by 400 mg per kilogram body weight rat per day. The weight change of rats, nickel concentration in organs, survival rate of rats, morphology of organs were observed in the experimental groups. The results obtained by the experiment were as follows: 1. The rats in the group treated with natural products had no effect on the weight gain. 2. The mean survival rate of rats in the nickel alone treatment group was 72.5% (6.6 mg/kg/day), 68.9% (13.2 mg/kg/day), 60.2% (19.8 mg/kg/day) respectively. 3. As for the amount of nickel accumulation in organs, it was the lowest amount treated with pine cone, pine root in that order. 4. When kidney and liver tissues were observed with as optical microscope, obvious change were visible in those tissues treated groups with Lonicerae and Aloe.

• 한국동물위생학회지
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• 제13권1호
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• pp.32-43
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• 1990

In order to investigate the clinico-pathological and immunohistochemical changes in the rats infected with Aujeszky’s disease virus(ADV), 100 heads of 4 weeks-old rats were inoculated intraperitoneally and intranasally, with the domestically isolated ADV, NYJ-1-87 strain, at $10^{3.0}$ or $10^{5.0}$$TCID_ {50}$/0.2ml. Results obtained through the experiments were summarized as follows : 1. Clinical signs such as dulness, anorexia, pruritus, fascial edema, dyspnea and ataxia were observed from the 2nd day and died at the 3rd to 5th day after ADV inoculation. By necropsy, congestion and hemorrhage were observed in the abdominal organs, while no specific changes were detected in the other organs. 2. In histopathological observation, degeneration and necrosis of the nervous cells, non-suppurative meningoencephalitis, microgliosis and perivascular cuffing were manifested in central nerve system but no specific changes were observed in the other organs. 3. By immunohistochemical staining using peroxidase antiperoxidase, the positive cells were detected in the tissues of kideny, spleen, urinary bladder and lung.

• Clinical and Experimental Reproductive Medicine
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• 제26권3호
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• pp.383-387
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• 1999

Objective: There is increasing evidence for the expression of rat in gene in several extrapituitary sites including testis and ovary. We also have demonstrated that the local LH expression in the rat epididymis and uterus, the major accessory sex organs in male and female reproductive system, respectively. Design: The present study was undertaken to elucidate whether the gene for LH receptor is expressed in rat uterus and whether the expressions of uterine LH and its receptor are differentially regulated during estrous cycle. Presence of the transcripts for rat LH receptor in the rat uterine tissue were confirmed by touchdown reverse transcription-polymerase chain reaction (RT-PCR). Results: In $LH{\beta}$ semi-quantitative RT-PCR, the highest expression level was shown in estrus stage. The level of ill receptor transcripts was also fluctuated during estrous cycle. In ovariectomized rats (OVX + Oil), the expressions of both uterine LH and LH-R were markedly reduced when compared to those from normal rats. Supplement with estradiol $17{\beta}$ to the ovariectomized rats (OVX + $E_2$) restored the expression levels of LH and its receptor to the levels in uteri from normal rats. Conclusion: Our findings indicated that 1) LH and its receptor gene are expressed in the rat uterus from cycling rats, 2) the expression of uterine LH and its receptor is mainly, if not all, under the control of ovarian sex steroid(s). These results suggested that the uterine LH may act as a local regulator with auto and/or paracrine manner, though the posibility that the pituitary LH may act directly on the regulation of uterine functions could not be discarded.

• Genomics & Informatics
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• 제16권1호
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• pp.2-9
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• 2018

Olfactory receptors (ORs) in mammals are generally considered to function as chemosensors in the olfactory organs of animals. They are membrane proteins that traverse the cytoplasmic membrane seven times and work generally by coupling to heterotrimeric G protein. The OR is a G protein-coupled receptor that binds the guanine nucleotide-binding $G{\alpha}_{olf}$ subunit and the $G{\beta}{\gamma}$ dimer to recognize a wide spectrum of organic compounds in accordance with its cognate ligand. Mammalian ORs were originally identified from the olfactory epithelium of rat. However, it has been recently reported that the expression of ORs is not limited to the olfactory organ. In recent decades, they have been found to be expressed in diverse organs or tissues and even tumors in mammals. In this review, the expression and expected function of olfactory receptors that exist throughout an organism's system are discussed.

• 한국환경보건학회지
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• 제22권3호
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• pp.8-16
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• 1996

Effects of water extract of aloe vera on lead-induced neurotoxicity were investigated in sciatic nerve isolated from rat. The mechanism on toxicity reduction by measuring activities of axonal enzymes, metabolism of myo-inositol in nerve, lead concentration in several organs and so on were further examimed. In the lead-treated rats, the transport rate of axonal enzyme, such as acetyl cholinesterase and choline acetyltransferase, was reduced by from 50% to 30% respectively. Reduction in myo-inositol concentration and $Na^+/K^+$ ATPase activity were also observed in sciatic nerve from lead-treated rat. However, the aloe extract administration significantly eliminated the impairment and maintained myo-inositol concentration to about 85% of normal level. Also aloe extract promoted the excretion rate of lead which is accumulated in blood, sciatic nerve and kidney. These results suggest that lead-induced neurotoxicity was significantly reduced by administration of aloe extract and the mechanism might be partly increase in kidney excretion rate of lead and parity normalization of $Na^+/K^+$ ATPase activity which is critical factor in order to keep nerve maintaining normal myo-inositol level.