• Title/Summary/Keyword: rapid preparation

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Assessment of polluted factors in aquatic environment using near infrared spectroscopy

  • Norio, Sugiura;Zhang, Yansheng;Wei, Bin;Zhang, Zhenya;Isoda, Hiroko;Maekawa, Takaaki
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1272-1272
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    • 2001
  • Eutrophication processes of aquatic environment are strictly correlated with the concentration levels of nitrogen, phosphorous, organic matter and biological parameters such as phytoplankton and chlorophylla (Tremel, 1996; Burns et al., 1997; Young et al. 1999; Wei et al.,2000). Accordingly, the monitoring and evaluation of these factors will provide useful information about the health of aquatic ecosystem. However, the traditional types of auqatic chemistry analysis and ecological monitoring of phytoplankton are time-consuming, costly, and further resulting in secondary pollution due to the use of reagents. NIR (near-infrared) spectroscopy, as a rapid, non-destructive, little sample preparation and reagents-free technology (Hildrum et al., 1992), has been extensively applied to the characterization of food (Osborne and Fearn, 1988), pharmaceutical (Morisseau and Rhodes, 1995) and textile materials (Clove et al.,2000). Currently, NIR technology has been used indirectly in inferring lake water chemistry by two approaches, suspended (Malley et al., 1996) or seston (Dabakk et al., 1999), and sediments (Korsman et al., 1992; Malley et al., 1999). In addition, the evaluation of trophic state and the identification of the key factors contributed to the trophication are the key step to restore the damaged aquatic environment. Moreover, an understanding of the factors, which regulate the algal proliferation, is crucial to the successful management of aquatic ecosystem. In the paper, NIR technology will be used to study the environmental factors affecting the algal proliferation in combination with the trophic state index and diversity index. This novel developed system can be applied in monitoring and evaluating allopathic water environment and provide real time information services for the aquatic environment management.

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A Tsunami Simulation Model based on Cellular Automata for Analyzing Coastal Inundation: Case Study of Gwangalli Beach (지진해일로 인한 해안 침수 분석을 위한 셀 오토마타 기반의 시뮬레이션 모델 개발: 광안리 해변 사례 연구)

  • Joo, Jae Woo;Joo, Jun Mo;Kim, Dong Min;Lee, Dong Hun;Choi, Seon Han
    • Journal of Korea Multimedia Society
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    • v.23 no.5
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    • pp.710-720
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    • 2020
  • Tsunami occurred by a rapid change in the ocean floor is a natural disaster that causes serious damage worldwide. South Korea seems to be out of the range of this damage, but it is quite possible that South Korea will fall within the range due to the long-distance propagation features of tsunami and many earthquakes occurred in Japan. However, the analysis and preparation for tsunami have been still insufficient. In this paper, we propose a tsunami simulation model based on cellular automata for analyzing coastal inundation. The proposed model calculates the range of inundation in coastal areas by propagating the energy of tsunami using the interaction between neighboring cells. We define interaction rules and algorithms for the energy transfer and propose a software tool to effectively utilize the model. In addition, to verify and tune the simulation model, we used the actual tsunami data in 2010 at Dichato, Chile. As a case study, the proposed model was applied to analyze the coastal inundation according to tsunami height in Gwangali Beach, a famous site in Busan. It is expected that the simulation model can be a help to prepare an effective countermeasure against tsunami and be used for a virtual evacuating training.

Preparation of $CuInSe_{2}$ Absorber Layer for Solar Cells by Non-vacuum Process (비진공방식에 의한 태양전지용 $CuInSe_{2}$ 광흡수층 제조)

  • Kim, Ki-Hyun;Ahn, Se-Jin;Yoon, Kyung-Hoon;Ahn, Byung-Tae
    • 한국신재생에너지학회:학술대회논문집
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    • 2007.06a
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    • pp.346-349
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    • 2007
  • 치밀한 $CuInSe_{2}$ (CIS) 태양전지용 광흡수층을 제조하기 위해 상용되는 출발물질을 이용하여 비진공방식인 paste coating 법으로 CIS 막을 제조하였다. 먼저 치밀한 CIS 막 제조를 위해 $Cu_{2}Se$의 액상 거동을 관찰하였다. 이러한 $Cu_{2}Se_{2}$의 액상거동을 위해 Se 분위기에서 Se 증발온도, 기판온도, 열처리시간 등을 다양하게 변화 시켰으며, Se 증발온도 $450^{\circ}C$, 기판온도 $550^{\circ}C$, 열처리시간 30분 그리고 수송가스 ($N_{2}$)를 20 sccm으로 최적조건을 형성하였다. 이러한 최적조건을 바탕으로 치밀한 CIS막을 위해 two-zone RIP (rapid temperature process) 방법으로 Se 분위기 안에서 셀렌화를 위한 열처리를 행하였다. 셀렌화를 위해 다양한 열처리시간에 따라 형성된 CIS 막은 CIS 광흡수층과 Mo 박막 사이에서 $MoSe_{2}$ 층이 형성되었지만, 균일한 CIS 막을 얻었으며 업자성장과 치밀화 거동을 관찰 하였다. 또한, CIS 막의 치밀화를 위해 Se 증발온도와 열처리시간을 고정하고 기판온도를 $600^{\circ}C$로 증가시켜 $Cu_{2}Se$의 액상거동을 관찰하였다. $600^{\circ}C$에서 형성된 CIS 막은 기판온도 $500^{\circ}C$의 시편보다 입자성장과 치밀화가 되었으나 기판으로 사용하는 soda-lime glass의 휨 현상이 발생하였다.

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Possible Use of NIR Spectroscopy for Soil Testing (토양검정에서 근적외 분광분석기의 이용 가능성)

  • Ryu, Kwan-Shig;Cho, Rae-Kwang;Park, Woo-Churl;Kim, Bok-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.4
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    • pp.273-277
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    • 2001
  • Traditional methods of chemical analysis for the soil properties take time and produce harmful waste. The purpose of this research was to evaluate an NIR technique for measuring some soil properties that are rapid and accurate in soil fertility assessments. The NIR instrument (InfraAlyzer 500, Bran & Luebbe Co.) was used for obtaining spectral data from 140 finely ground soil for calibrations and validation estimating pH, CEC, extractable Ca, Mg, K, $SiO_2$, humic acid and EC. Partial least square regression analysis was used to develop a calibration of NIR spectroscopy method. The results indicated that NIR spectroscopy could be used as a routine nondestructive method quantitatively determining soil chemical properties quickly. However the NIR technique may require sample preparation to obtain even diffuse reflection spectra from the soil and data manipulations to obtain optimal predictions.

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HPLC Assay and Renal Excretion Characteristics of Theophylline and Its Metabolites in Rat (테오필린과 그 대사체의 HPLC 동시 정량 및 신(腎) 배설 특성)

  • Kuh, Hyo-Jeong;Shim, Chang-Koo;Lee, Min-Hwa;Kim, Shin-Keun
    • Journal of Pharmaceutical Investigation
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    • v.21 no.1
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    • pp.33-41
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    • 1991
  • A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of theophylline(TP) and its metabolites, 1-methyluric acid (1-MU) and 1,3-dimethyluric acid (1,3-DMU), in rat plasma and urine. An $100\;{\mu}l$ aliquot of a plasma or urine sample was mixed with $250\;{\mu}l$ of acetonitrite and vortexed. After centrifugation, $200\;{\mu}l$ (plasma) or $20\;{\mu}l$ (urine) aliquot of the supernatant was dried by $N_2$ stream and redissolved in $100\;{\mu}l$ (plasma) or $200\;{\mu}l$ (urine) of the mobile phase. A $20\;{\mu}l$ of the mobile phase solution was injected onto a $C_{18}$ reversed-phase column. The column was maintained at $45^{\circ}C$ by the aid of electric heating jacket. The mobile phase was a 3%(v/v) methanol solution in deionized water which contains sodium acetate (100 mM) and tetrabutyl ammonium hydroxide (4 mM). pH of the mobile phase was adjusted 4.5 by the addition of acetic acid. Detection limits for TP, 1-MU, and 1,3-DMU in plasma were 0.2, 0.1 and $0.1\;{\mu}/ml$, respectively and the corresponding values in urine were all $5\;{\mu}g/ml$. Inter- and intra-day variability of the assay for all compounds in the plasma samples was less than 5.5 and 3.8%, respectively. The retention times for 1-MU, 1,3-DMU, and TP were approximately 7, 8.5 and 18 min, respectively. Sample preparation procedure used in this method was simple, rapid and reproducible. Renal clearance of TP and its metabolites in rats showed plasma concentration dependency indicating renal tubular secretion and reabsorption of them.

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The Development of Cisplatin Analysis Method in Plasma by Flameless Atomic Absorption Spectrometry (FAAS를 이용한 혈중 Cisplatin의 분석법 개발)

  • Paik, Man-Jeong;Lim, Ho-Sub;Jeong, Mi-Jin;Lee, Kyoung-Ok;Shin, Ho-Sang
    • Analytical Science and Technology
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    • v.11 no.4
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    • pp.243-247
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    • 1998
  • Cisplatin is a platinum-containing antitumor agent with nephrotoxic and neurotoxic side effects. An analytical method for measuring cisplatin in plasma by FAAS was developed, which is rapid, simple and need no sample preparation. The linearity test of calibration curve in the range of 20~1000 ng/mL showed good correlation coefficient of r=0.999. The result of accuracy test appeared to be relative standard deviation of < 5.0% at concentration range from 50 to 1000 ng/mL. When $200{\mu}L$ of plasma was used, detection limit was 10 ng/mL. Therefore, it can be applied for the monitoring in plasma for optimal condition of treatment and reduce of toxicity.

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Quantitative Analysis of Acylcarnitines and Amino Acids Using ESI-MS/MS (ESI-MS/MS를 이용한 아실카르니틴과 아미노산의 정량분석)

  • Kim, Hohyun;Han, Sang-Beom;Yoon, Hye-Ran
    • Analytical Science and Technology
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    • v.14 no.5
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    • pp.384-391
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    • 2001
  • In this study, a new quantitative analytical method has been developed for the rapid determination of acylcarnitines and amino acids in human blood using electrospray ionization / tandem mass spectrometry (ESI-MS/MS). Acylarmitines and amino acids were analyzed by tandem mass spectrometry after conversion to their butylesters through treatment with 3N butanolic hydrogen chloride. Acylcrnitines were analyzed using precursor 85 ion scan and alanine, valine, leucine/isoleucine, methionine, phenylalanine, tyrosine, aspartic acid and glutamic acid were analyzed using neutral loss 102 scan, ornitine and citrulline were analyzed neutral loss 119 scan, glycine was analyzed using neutral loss 56 scan, arginine was analyzed using neutral loss 161 scan and argininosuccinic acid was analyzed product ion 459 scan. This method reduced sample preparation time compared to that with conventional amino acid analyzer and liquid chromatography, with high sensitivity and good reproducibility.

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Technical Trends and Issues Related to Maintenance/Inspection Drones for Ships or Offshore Plants (선박/해양플랜트 유지보수 점검용 드론의 기술현황과 과제)

  • Kim, Kyunghwa;Chun, Kangwoo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.5
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    • pp.585-594
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    • 2017
  • Drones, which were first developed for the military use, have been widely applied in various industrial fields through the rapid development of element technology. Following this trend, the marine industry has started to utilize drones for maintenance and inspection purposes, especially, in Europe. To extend the use of drones in the marine industry, this paper first discusses the necessity of drones for the marine use by presenting problems related to maintenance and inspection works on ships and offshore plants. In addition, the technical levels of advanced countries where drones have been commercialized already in the marine industry are mentioned. Furthermore, technical challenges that must be solved are explained in preparation for a drone era in the marine industry. Lastly, the potential growth of the drone market in the marine industry is considered.

Comparison of Short-Term Toxicity Tests Based on Feeding Behavior and Temperature Control by Ceriodaphnia dubia (Ceriodaphnia dubia의 먹이섭생 기작과 온도조절에 근거한 급성독성조사법의 비교)

  • Park, Jong-Ho;Lee, Sang-Ill;Cho, Young-Oak
    • Journal of Korean Society on Water Environment
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    • v.20 no.1
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    • pp.48-54
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    • 2004
  • Two methods, a Ceriodaphnia algal uptake suppression test (CAUST) and a new toxicity test based on temperature control (TTBTC) which are based on feeding behaviour and temperature control, respectively, were developed and compared for the adoption as the better methodology for short-term toxicity screening. As previously published by Lee et aI., (1997), the CAUST method is based on the feeding behaviour of C. dubia and requires as little as 1 hour of contact time between C. dubia neonates and toxicant. However, even though CAUST requires only 1 hour of contact time, this method still take many hours for the preparation and measurement. Before the test starts, neonate digestive tracts were cleared by feeding yeast to the daphnids, Neonates were then exposed to toxicant, followed by addition of Scenedesmus subspiatus into the bioassay vessels. Daphnids were examined under the bright-field microscope with the presence of algae (indicated by a green colored digestive tract) or the absence of algae. Uptake indicated no toxic effect, whereas, absence of uptake indicated toxic inhibition. Unlike CAUST, the newly developed method (TTBTC) is based on just temperature control for the toxicity test of C. dubia. Initially, neonates are exposed to toxicants while the temperature of water bath containing media increased to $35.5^{\circ}C$. After 1.25 hour of contact time, the number of the daphnids, either live (no toxic effect) or dead (toxic effect), is counted without the aid of any instrument. In both methods, median effective concentrations ($EC_{50}$ values) were computed based on the results over a range of dosed toxicant concentrations. It showed that TTBTC was as sensitive as the standard 48-hour acute bioassay and CAUST. TTBTC and CAUST were much more sensitive than the I-hour I.Q. test and 30-minute Microtox. This study indicates that TTBTC is an easier and more rapid toxicity test than the standard 48-hour acute bioassay and even CAUST.

Management of Femoropopliteal Vascular Injuries after Trauma: Surgical Outcomes (외상 후 대퇴-오금 혈관손상의 치료: 수술 성적)

  • Chang, Sung Wook;Han, Sun;Ryu, Kyoung Min;Ryu, Jae-Wook
    • Journal of Trauma and Injury
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    • v.28 no.1
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    • pp.15-20
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    • 2015
  • Purpose: Vascular injuries caused by traffic, industrial accidents and by outside activities have increased in Korea. Especially, vascular injuries to the extremities can lead to limb loss and even mortality if they are not appropriately treated. The aim of the study was to evaluate the surgical outcomes of femoropopliteal vascular management after trauma. Methods: The medical records of 12 patients with femoropopliteal vascular injuries who were treated at Dankook University Hospital from 2011 to 2013 were reviewed. Iatrogenic vascular injuries were excluded. The clinical data including the causes of injury, associated injuries and surgical outcomes were analyzed retrospectively. Results: All patients were male, with a mean age of $46.8{\pm}16.3years$ (range: 26~69 years). The causes of vascular injuries were four traffic accidents, three industrial accidents, two iron plates, one outside activity, one glass injury and one knife injury. The average transit time between the place of the accident place and the emergency department was $3.0{\pm}2.1$ (0.5~12.5) hours, and the average preparation time for surgery was $8.0{\pm}6.7$ (1.7~23.3) hours. The anatomic injuries included the popliteal vessel in seven cases and the femoral vessel in five cases. The average Injury Severity Score (ISS) was $12.0{\pm}5.0$ (5~17), and the average Mangled Extremity Severity Score (MESS) was $5.7{\pm}2.1$ (2~9). The operation methods were four interposition grafts, three end-to-end anastomoses, two direct repairs and three patch angioplasties. One case required amputation of the injured extremity. Conclusion: Early recognition and revascularization of the injured vessel are mandatory to reduce limb loss and to obtain satisfactory outcomes. Therefore, careful/rapid evaluation of the vascular injuries and timely/successful surgical treatment are the keys to salvaging an injured limb.

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