• Journal of Microbiology and Biotechnology
• /
• 제18권11호
• /
• pp.1841-1847
• /
• 2008

Vibrio vulnificus is a causative agent of serious diseases in humans, resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for the V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1 g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium, using the rpoS gene in pure cultures and in infected oyster tissues.

• Imaging Science in Dentistry
• /
• 제51권3호
• /
• pp.237-242
• /
• 2021

Purpose: The aim of this study was to analyse and review deep learning convolutional neural networks for detecting and diagnosing early-stage dental caries on periapical radiographs. Materials and Methods: In order to conduct this review, the Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA) guidelines were followed. Studies published from 2015 to 2021 under the keywords(deep convolutional neural network) AND (caries), (deep learning caries) AND (convolutional neural network) AND (caries) were systematically reviewed. Results: When dental caries is improperly diagnosed, the lesion may eventually invade the enamel, dentin, and pulp tissue, leading to loss of tooth function. Rapid and precise detection and diagnosis are vital for implementing appropriate prevention and treatment of dental caries. Radiography and intraoral images are considered to play a vital role in detecting dental caries; nevertheless, studies have shown that 20% of suspicious areas are mistakenly diagnosed as dental caries using this technique; hence, diagnosis via radiography alone without an objective assessment is inaccurate. Identifying caries with a deep convolutional neural network-based detector enables the operator to distinguish changes in the location and morphological features of dental caries lesions. Deep learning algorithms have broader and more profound layers and are continually being developed, remarkably enhancing their precision in detecting and segmenting objects. Conclusion: Clinical applications of deep learning convolutional neural networks in the dental field have shown significant accuracy in detecting and diagnosing dental caries, and these models hold promise in supporting dental practitioners to improve patient outcomes.

• Korean Journal of Remote Sensing
• /
• 제38권6_3호
• /
• pp.1861-1869
• /
• 2022

Recent climate change can increase the frequency and damage of wildfires worldwide. It can also lead to the deterioration of the forest ecosystem and increase casualties and economic loss. Satellite-based indices for forest damage can facilitate an objective and rapid examination of burned areas and help analyze inaccessible places like North Korea. In this letter, we conducted a detection of burned areas in North Korea using the traditional Normalized Burn Ratio (NBR), the Normalized Difference Vegetation Index (NDVI) to represent vegetation vitality, and the Fire Burn Index (FBI) and Forest Withering Index (FWI) that were recently developed. Also, we suggested a strategy for the satellite-based detection of burned areas in the Korean Peninsula as a result of comparing the four indices. Future work requires the examination of small-size wildfires and the applicability of deep learning technologies.

• Korean Journal of Food Science and Technology
• /
• 제48권5호
• /
• pp.424-429
• /
• 2016

Globally, obesity has been recognized as a serious health problem. Recently, slimming foods for weight loss and maintenance were found to contain anti-obesity drugs, their analogues, and prohibited ingredients. To avoid inspections by the Government, structurally modified analogs have been continuously synthesized. For rapid determination of adulterated slimming products, we simultaneously analyzed 21 illegal compounds using liquid chromatography (LC) equipped with photo diode array, and LC coupled with tandem mass spectrometry. The validation of the method was performed with regard to selectivity, linearity, limit of detection, limit of quantification, precision, and accuracy. We purchased 128 samples from the Korean market and online sources in the year 2015. In 31 samples, 3 illegal compounds were detected as follows: 9.9-135.3 mg/g of sibutramine, 0.2-17.5 mg/g of yohimbine, and 1.8 mg/g of icariin. This simultaneous detection method might prove to be a simple and rapid analysis for monitoring illegal compounds in slimming foods.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• 제18권7호
• /
• pp.417-422
• /
• 2017

One of the most frightening aspects of weapons of mass destruction (WMD) is their ability to cause death in very small quantities without being visible to the public. The military authorities are making considerable effort to ensure the survivability of the combatants in the event of NBC(Nuclear, Biological and Chemical) contamination. Therefore, in this study, modules were developed for the measurement of the positive pressure and for the detection of the chemicals used for the control of the various shut-off valves used in an NBC shelter. In addition, a high performance gas tight shut-off valve was developed that can overcome the disadvantages associated with manual manufacturing, such as the occurrence of defective products and high manufacturing cost. By applying the positive pressure measurement and chemical detection modules, this valve was able to be used to control the facility. The developed gas-tight shut-off valve maintained airtight characteristics at a pressure loss of 28[Pa] at the prescribed wind velocity and an internal pressure of 30[kPa]. It is expected to be possible to control the gas-tight shut-off valve through the remote measurement of the positive pressure, thereby ensuring the foreign independence of import substitution and defense related technology in the future. In addition, by installing these valves in all of the intake ports or exhaust ports connected to the outside of the NBC shelter, it is possible to prevent the damage resulting from the rapid inflow of the storm pressure caused by conventional weapons and nuclear explosions, thereby protecting the people and equipment in the shelter.

• Applied Biological Chemistry
• /
• 제49권4호
• /
• pp.315-321
• /
• 2006

Inhibitors of acetylcholinesterase(AchE), such as organophosphates and carbamates, interfere the action of AchE in nerve and may lead to a severe impairment of nerve functions or even death. Therefore, insect AchE is the biological target of predominant insecticides used in agriculture. Biosensors are sensitive and can be used as dispoisable sensors for environmental control. In recent years, the use of AchEs in biosensor technology has gained enormous attention, in particular with respect to insecticide detection. The principle of biosensors using AchE as a biological recognition element is based on the inhibition the catalytic activity by the agents to be detected. We here present a strip-type biosensor based on AchE inhibition. In this study, acetylcholinesterase and PVA-SbQ(polyvinyl alcohol functionalized with methyl pyridinium methyl sulfate) were co-immobilized on immobilone-P membranes. Immobilization of the enzymes showed a stability in 6 months without activity loss in $4^{\circ}C$ storage. Enzymes immobilized on surfaces of membrane responded to organophosphates and carbamate more sensitivitive than enzyme in solution. Organophosphates and carbamates concentrations could be detected by entrapped and surface immobilized enzymes, in 5 min. For chlorpyrifos, carbofuran, cabaryl, and methidathion, the detection limits of AChE-strip were similar to that of HPLC/GC method.

• Journal of Microbiology and Biotechnology
• /
• 제30권7호
• /
• pp.955-961
• /
• 2020

Lactic acid bacteria (LAB) have caused many microbiological incidents in the brewing industry, resulting in severe economic loss. Meanwhile, traditional culturing method for detecting LAB are time-consuming for brewers. The present review introduces LAB as spoilage microbes in daily life, with focus on LAB in the brewing industry, targeting at the spoilage mechanism of LAB in brewing industry including the special metabolisms, the exist of the viable but nonculturable (VBNC) state and the hop resistance. At the same time, this review compares the traditional and novel rapid detection methods for these microorganisms which may provide innovative control and detection strategies for preventing alcoholic beverage spoilage, such as improvement of microbiological quality control using advanced culture media or different isothermal amplification methods.

• Food Science of Animal Resources
• /
• 제35권1호
• /
• pp.121-129
• /
• 2015

${\beta}$-agonists are anabolic compounds that promote fat loss and muscle gain, and their administration to livestock may provide economic benefits by increasing growth rate and feed efficiency. For these reasons, ${\beta}$-agonists are also commonly added to livestock feed as growth promoters. This can introduce a significant risk of secondary human poisoning through intake of contaminated meat. A new method for the simultaneous determination of three ${\beta}$-agonists (clenbuterol, ractopamine, and zilpaterol) was developed in this study and applied to various meat samples. The limits of quantification, derived through a validation test following Codex guidelines, were $0.2{\mu}g/kg$ for clenbuterol and zilpaterol, and $0.4{\mu}g/kg$ for ractopamine. The average recoveries for clenbuterol, ractopamine, and zilpaterol ranged from 109.1% to 118.3%, 95.3% to 109.0%, and 94.1% to 120.0%, respectively. The recovery and coefficient of variation (CV) values fell within the acceptable range according to the Codex guidelines. This method reduced the analysis time without decreasing detection efficiency by modifying the pretreatment steps. This method could be utilized to manage the safety of imported meat products from countries where zilpaterol use is still permitted, thereby improving public health and preventing ${\beta}$-agonist poisoning due to secondary contamination.

• Journal of Gastric Cancer
• /
• 제20권1호
• /
• pp.29-40
• /
• 2020

Purpose: Gastrointestinal stromal tumors (GISTs) frequently harbor activating gene mutations in either KIT or platelet-derived growth factor receptor A (PDGFRA) and are highly responsive to several selective tyrosine kinase inhibitors. In this study, a targeted next-generation sequencing (NGS) assay with an Oncomine Focus Assay (OFA) panel was used for the genetic characterization of molecular targets in 30 Korean patients with GIST. Materials and Methods: Using the OFA that enables rapid and simultaneous detection of hotspots, single nucleotide variants (SNVs), insertion and deletions (Indels), copy number variants (CNVs), and gene fusions across 52 genes relevant to solid tumors, targeted NGS was performed using genomic DNA extracted from formalin-fixed and paraffin-embedded samples of 30 GISTs. Results: Forty-three hotspot/other likely pathogenic variants (33 SNVs, 8 Indels, and 2 amplifications) in 16 genes were identified in 26 of the 30 GISTs. KIT variants were most frequent (44%, 19/43), followed by 6 variants in PIK3CA, 3 in PDGFRA, 2 each in JAK1 and EGFR, and 1 each in AKT1, ALK, CCND1, CTNNB1, FGFR3, FGFR4, GNA11, GNAQ, JAK3, MET, and SMO. Based on the mutation types, majority of the variants carried missense mutations (60%, 26/43), followed by 8 frameshifts, 6 nonsense, 1 stop-loss, and 2 amplifications. Conclusions: Our study confirmed the advantage of using targeted NGS with a cancer gene panel to efficiently identify mutations associated with GISTs. These findings may provide a molecular genetic basis for developing new drugs targeting these gene mutations for GIST therapy.

• Journal of computational fluids engineering
• /
• 제22권1호
• /
• pp.37-42
• /
• 2017

Infrared signals are frequently used to detect objects exposed to wide variety of environmental conditions. Detection by infrared signature is accomplished by distinguishing objects by using the IR radiant contrast between objects and the background. There are several methods of estimating the IR radiant contrast. The inverse distance weighting method, which is one of the IR radiant contrast estimation method using the effect of distance from objects, is known to be an effective way to analyze radiant contrast for complex backgrounds. However this method has a disadvantage of requiring a long calculation time. In this study we propose a statistical method of estimating the IR radiant contrast by using randomly selected pixels of arbitrary number among background pixels to reduce calculation time. Some measured IR images in MWIR and LWIR regions are used to test the applicability of the method proposed and we found that the proposed method is very effective in determining the IR radiant contrast showing very rapid estimation with minar accuracy loss.