• Title/Summary/Keyword: randomized block design with replications

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Nitrogen Fixation of Legumes and Transfer to Grasses in Spring Paddy Soil (춘계답리작 토양에서 두과의 질소고정과 이의 화본과로의 이동)

  • Lee, Hyo-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.3
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    • pp.167-172
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    • 2007
  • Manure recycle is an emerging issue in agriculture in Korea these days. Farmers are keeping eye on legume mixture with grasses for nitrogen fixation and transfer to companion crops by legumes. We had a trial to investigate the effect of different legume mixtures on nitrogen fixation and transfer in spring soil. The treatment was arranged in a randomized complete block design with three replications. Three different mixtures were used(rye+hairy vetch, Italian ryegrass+crimson clover, oat+pea) and sowed in pots with paddy soil from western part of Korea(Seo Chon County). Pots with rye+vetch were transplanted from field of RDA(rural development administration) in Suwon on 2 February 2007 and other mixture treatments were sowed on early March with different sowing rate(7:3=Italian:crimson, 6:4=oat:pea). $(^{15}NH_4)_2SO_4$ solution at. $99.8\;atom%^{15}N$ was applied to the each pot at the rate of $2kg\;N\;ha^{-1}$. Application was done on 6 April at rye+vetch pots and remainder were applied on April 16. Forage were harvested from each pot at ground level in heading stage and separated into legume and grass. Total N content and $^{15}N$ value were determined using a continuous flow stable isotope ratio mass spectrometry. DM yield of rye+vetch, Italian+crimson and oat+pea were 6,607, 3,213 and 4,312 kg/ha, respectively. Proportion of N from fixation was 0.73(rye+vetch), 0.42(Italian+crimson) and 0.93(oat+pea). The percentages of N transfer from legume to grass were from 61% to 24% in different method by treatment and -35% to 21% in isotope dilution method.

Effect of Mixture Types with Festulolium braunii (Festuca pratensis Huds. × Lolium multiflorum Lam.) on Dry Matter Yield and Quality (Festulolium braunii (Festuca pratensis Huds. × Lolium multiflorum Lam.)의 혼파유형이 목초의 건물수량과 품질에 미치는 영향)

  • Lee, In Duk;Lee, Hyung Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.4
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    • pp.311-316
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    • 1998
  • This study was conducted to evaluate the dry matter yield and quality of mixture types with Festulolium braunii (Festuca pratensis Huds. ${\times}$ Lolium multiflorum Lam.) as a forage sources. The experiment was arranged in a randomized block design with three mixture types: (1) Festulolium braunii (seeding rate, FB 50%)+orchardgrass (OG 50%), (2) Festulolium braunii (FB 80%)+red clover(RC 20%), and (3) Festulolium braunii (FB 20%)+orchardgrass (OG 50%)+tall fescue(TF 20%)+white clover (WC 10%) and four replications. This study carried out from Sep. 1995 to May 1998 at Chungnam University. Throughout this experiment, the dry matter yields and forage quality were observed. The DM yield of FB+RC mixtures was higher than that of other mixture types, but there were no significant difference among mixture types. The FB+RC mixtures was higher than the other mixture types in the CP content(P<0.05). However, the NDF and ADF content of FB+RC mixtures were significantly lower than those of other mixture types(P<0.05). The CP and DDM yields of FB+RC mixtures were higher than those of other mixture types(P<0.05). In addition, the FB percentage of total herbage was ranged from 51% to 58% in all mixture types at the last cutting time in 1997. Based on the results mentioned above, it appears that FB has enough of possibility in mixture with other grasses, especially good mixed with RC.

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Dietary Requirement of True Digestible Phosphorus and Total Calcium for Growing Pigs

  • Ruan, Z.;Zhang, Y.-G.;Yin, Y.-L.;Li, T.-J.;Huang, R.-L.;Kim, S.W.;Wu, G.Y.;Deng, Z.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.8
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    • pp.1236-1242
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    • 2007
  • Sixty healthy growing pigs ($Duroc{\times}Landrace{\times}Yorkshire$ with an average BW of 21.4 kg) were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. Pigs were assigned randomly to one of five dietary treatments (12 pigs/diet), representing five levels of TDP (0.16%, 0.20%, 0.23%, 0.26% and 0.39%). There were three replications per treatment, with four pigs (2 barrows and 2 gilts) in each replication (2 pigs/pen) A randomized-block design was used, with pen as the experimental unit. Experimental diets were formulated to provide the 5 TDP levels with a total calcium (Ca) to TDP ratio of 2:1, and offered to pigs at 5% BW for 28 d. The total Ca contents of the five diets were 0.33, 0.38, 0.45, 0.51 and 0.79%, respectively. During the 28-d experimental period, the ADG of pigs was affected by dietary TDP levels as described by Equation 1: y = $-809,532x^4+788,079x^3-276,250x^2+42,114x-1$,759; ($R^2$ = 0.99; p<0.01; y = ADG, g/d; x = dietary TDP, %). The feed:gain ratio for pigs was affected by dietary TDP levels as described by Equation 2: y = $3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2$ = 0.99; p<0.01; y = feed:gain ratio; x = dietary TDP, %). Total P concentrations in serum were affected by dietary TDP levels as described by Equation 3: y = $-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ ($R^2$ = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest feed:gain ratio (1.07), and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet (e.g., 5.1 g/day for a 30-kg pig that consumed 1.5 kg feed daily) at a total Ca to TDP ratio of 2:1.

Influence of various levels of milk by-products in weaner diets on growth performance, blood urea nitrogen, diarrhea incidence, and pork quality of weaning to finishing pigs

  • Yoo, S.H.;Hong, J.S.;Yoo, H.B.;Han, T.H.;Jeong, J.H.;Kim, Y.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.5
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    • pp.696-704
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    • 2018
  • Objective: This study was conducted to evaluate various levels of milk by-product in weaning pig diet on growth performance, blood profiles, carcass characteristics and economic performance for weaning to finishing pigs. Methods: A total of 160 weaning pigs ([Yorkshire${\times}$Landrace]${\times}$Duroc), average $7.01{\pm}1.32kg$ body weight (BW), were allotted to four treatments by BW and sex in 10 replications with 4 pigs per pen in a randomized complete block design. Pigs were fed each treatment diet with various levels of milk by-product (Phase 1: 0%, 10%, 20%, and 30%, Phase 2: 0%, 5%, 10%, and 15%, respectively). During weaning period (0 to 5 week), weaning pigs were fed experimental diets and all pigs were fed the same commercial feed during growing-finishing period (6 to 14 week). Results: In the growth trial, BW, average daily gain (ADG), and average daily feed intake (ADFI) in the nursery period (5 weeks) increased as the milk by-product level in the diet increased (linear, p<0.05). Linear increases of pig BW with increasing the milk product levels were observed until late growing period (linear, p = 0.01). However, there were no significant differences in BW at the finishing periods, ADG, ADFI, and gain:feed ratio during the entire growing-finishing periods. The blood urea nitrogen concentration had no significant difference among dietary treatments. High inclusion level of milk by-product in weaner diet decreased crude protein (quadratic, p = 0.05) and crude ash (Linear, p = 0.05) of Longissimus muscle. In addition, cooking loss and water holding capacity increased with increasing milk product levels in the weaner diets (linear, p<0.01; p = 0.05). High milk by-product treatment had higher feed cost per weight gain compared to non-milk by-products treatment (linear, p = 0.01). Conclusion: Supplementation of 10% to 5% milk by-products in weaning pig diet had results equivalent to the 30% to 15% milk treatment and 0% milk by-product supplementation in the diet had no negative influence on growth performance of finishing pigs.

Growth, Hay Yield and Chemical Composition of Cassava and Stylo 184 Grown under Intercropping

  • Kiyothong, K.;Wanapat, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.799-807
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    • 2004
  • The objective of this field experiment was to investigate the growth, hay yield and chemical composition of cassava and stylo 184 grown under intercropping. The experiment was arranged in a Randomized Complete Block (RCB) design with 5 treatments and 4 replications. The treatments were: sole crop cassava (C); sole crop stylo 184 (S) and three intercropping treatments comprising an additive series of one (SC), two (SSC) and three (SSSC) rows of stylo 184 to one row of cassava. The results showed that leaf area per plant (LA) of cassava was significantly higher (p<0.05) in the sole crop relative to the intercropping treatments. Both total hay yield and CPDM yield were significantly higher (p<0.05) for C treatment and lower (p<0.05) for SSSC treatment. The total hay yield and CPDM yield were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments. At the first and second harvests, CP content was similar among treatments; while at third and fourth harvests, CP contents were significantly greater (p<0.05) for the intercropping treatment relative to the sole crop. At the first and second harvest, NDF contents were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments, whereas NDF contents were similar among intercropping treatments. Leaf area of stylo 184 at first and second harvest were significantly greater (p<0.05) for C, SC and SSC as compared with the SSSC treatments. At each harvesting, there were no significant differences in ash, CP, NDF, ADF and ADL contents of stylo 184 hay between the sole crop and intercropping treatments, except for the first harvest. ADF contents were significantly greater (p<0.05) in S, SC treatments relative to SSC and SSSC treatments. Both collective hay yield and CPDM yield of cassava and stylo 184 were significantly greater (p<0.05) for the SSC treatment and significantly lower (p<0.05) for the S treatment. Collective hay yield and CPDM yield were significantly greater (p<0.05) for the intercropping treatments relative to the sole crop. Based on this research, it was concluded that stylo 184 showed potential for intercropping with cassava. Intercropping cassava with stylo 184 has beneficial effects and can improve foliage biomass yield and soil fertility, which would be a more sustainable system than growing the cassava as a pure stand. In terms of hay yield and CP production, two rows of stylo 184 to one row of cassava could be the optimal pattern for this intercropping system.

Effect of Fermented Pig Slurry Treated with Probiotics on the Productivity of Sorghum $\times$ Sudangrass Hybrid(Sorghum bicolor L. Moench) (미생물제제 이용 처리 발효돈분액비 시용이 수수교잡종 (Sorghum bicolor L. Moench)의 생산성에 미치는 영향)

  • 박남건;고서봉;고문석
    • Journal of Animal Environmental Science
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    • v.8 no.1
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    • pp.35-42
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    • 2002
  • This study was carried out to determine the effect of chemical fertilizer and two fermented types of pig slurry on the total dry matter yield and quality of sorghum $\times$ sudangrass hybrid (Sorghum bicolor L. Moench) and chemical properties of soil after harvest in Jeju area. Three treatments consisted of chemical fertilizer (CF) 200kg 7kg Nha $^1$, aerobic fermented pig slurry (PS)200kg 7kg Nha $^1$, and aerobic fermented pig slurry treated with probiotics (PS+P) 200kg 7kg Nha $^1$were arranged in a randomized block design with three replications. The results obtained are summarized as follows. The heights of plant applied fermented pig slurry were slightly taller than those of plants applied chemical fertilizer during early growing stage, but there was no difference among treatments when the plants were harvested. The total forage dry matter yields were in the range of 14,848~ 15,42kg/ha, but they were not significantly different. Also, CP, NDF, ADF and mineral contents in the forage(% of DM basis) did not differ among treatments. The pH of soil was ranged from 5.35 to 5.63, but it was not significantly different. However, the content of available $P_2O_5$ of soil was higher(P<0.05) in chemical fertilizer treatment than that of soil in fermented pig slurry treatments. The content of K was higher(p<0.05) when ffrrmented pig slurry treated with probiotics was applied after the 1st harvest. but it was not different among treatments after the and harvest.

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Yield and Ecological Characteristics of Soybean in Drained-Paddy Field (파종기별 논콩재배의 수량 및 생태적 특성)

  • Cho, Jin-Woong;Lee, Sok-Young;Kang, Soon-Kyung;Kim, Choong-Soo
    • Korean Journal of Agricultural Science
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    • v.33 no.2
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    • pp.141-147
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    • 2006
  • This study was conducted on a commerce silt loam soil at paddy field in the southwestern Korea ($36^{\circ}N$ lat). In the study of planting time, seed were hand planted at 24 May, 14 June and 5 July, and at 24 May, 14 June and 5 July in 2005, respectively. Two seedlings plants per hill were taken prior to V3 stage. Fertilizer was applied prior to plant at a rate of 3.0 - 3.0 - 3.4 g (N - P - K) per square meter. Experimental design was a randomized complete block in a split plot arrangement with three replications. Yield was significantly affected by different of the planting times and cultivars. The seed yield in planting time was the highest on late in May 24 but was the lowest on July 5. Also, the plant time significantly affected on increasing pod and seed number and seed weight. The days from emergence to flowering and maturity was reducing tendency with late planting time. The highest and lowest total dry matter production per square meter appeared at late in May and early in July with planting time, respectively. RGR, LAR and SLA was increased with late planting time. Photosynthetic rate at each planting time was not significant on the expanded the highest leaf position but at seed development stage, it was higher photosynthetic rate at May 24 than that of late planting time on $7^{th}$ laef position from the basal part.

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Effects of Inoculants on the Quality of Round Baled Grass Silage (유산균제 첨가가 라운드베일 목초 사일리지의 품질에 미치는 영향)

  • Kim, Jong-Geun;Chung, Eui-Soo;Seo, Sung;Ham, Jun-Sang;Yoon, Sei-Hyung;Lim, Young-Chul
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.3
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    • pp.139-146
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    • 2006
  • This experiment was conducted to determine the effect of microbial inoculant on the quality of round baled grass silage at experimental field of Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three different inoculant (control, inoculant A, B and C). The contents of ether extract(EE), crude protein(CP) and in vitro dry matter digestibility(IVDMD) in inoculant treatment plots were higher than those in control. Silages treated by Inoculant A, Band C had significantly lower acidity than that of control silage (p<0.05). Dry matter(DM) content of control silage was higher than those of inoculant treated silage. There were significant differences in organic acid contents among treatments(p<0.05). Lactic acid was increased with inoculant treatment, but, acetic and butyric acid was decreased. The DM loss of all silages were decreased with inoculant treatment and quality grade of inoculant treated silage was higher than that of control. Results of this study indicate that addition of microbial inoculant will improve the fermentation and quality of round baled grass silage.

Forage Yield and Quality of Oats as Affected by Different Types and N Rates of Liquid Manure (액상분뇨의 종류 및 N 시용량이 연맥의 사초수량과 품질에 미치는 영향)

  • Shin, D.E.;Kim, D.A.;Choi, K.J.;Kim, W.H.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.121-126
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    • 1999
  • Manure management is a top priority on both dairy and swine farrns at present. This experiment was carried out to determine the effect of different types and N rates of liquid manure on forage yield and quality of oats(Avena sativa L.) in Suweon, 1997. Seven treatments consisting of chemical fertilizer $120kg\;N\;ha^{-1}$, liquid cattle manure 120, 240 and $360kg\;N\;ha^{-1}$, and liquid swine manure 120, 240 and $360kg\;N\;ha^{-1}$ were arranged in a randomized complete block design with three replications. As increasing N rate of the liquid manure, the plant height was increased, but dry matter content of oats was decreased as increasing liquid manure N rate. There were significant differences in dry matter yield of oats among the liquid manure N rates(P<0.05). Crude protein(CP) content of oats ranged from 14.7 to 24.1% (P<0.05), and CP yield was orderly ranked as liquid swine manure $360kg\;N\;ha^{-1}$>liquid cattle manure $360kg\;N\;ha^{-1}$>liquid swine manure $240kg\;N\;ha^{-1}$(P<0.05). Acid detergent fiber(ADF) content of the liquid cattle manure treatment showed a slightly higher than that of the liquid swine manure treatment(P<0.05). Neutral detergent fiber(NDF) content of the liquid cattle manure treatment was lower than that of the liquid swine manure treatment(P<0.05). Relative feed value(RFV) of the liquid cattle manure treatment decreased with increasing liquid manure N rates compared with that of the liquid swine manure treatment. Based on the results of this experiment, it is indicates that to increase the N rates from liquid manure application could be obtained from the increase of forage yield.

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The Effect of Developed SCB Liquid Fertilizer on the Growth of Kentucky Bluegrass (성분이 추가된 SCB저농도액비가 켄터키블루그래스의 생육에 미치는 효과)

  • Ham, Suon-Kyu;Kim, Young-Sun;Lim, Hye-Jung
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.73-78
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    • 2011
  • This study was conducted to evaluate the effect of developed SCB (DSCB) liquid fertilizer produced by adding N, P and K at SCB liquid fertilizer on the growth of kentucky bluegrass. Two different N sources used in DSCB were ammonium sulfate (DSCB-A) and urea (DSCB-U), respectively. Fertilizer treatments were designed as follows; non-fertilizer (NF), control (CF; chemical fertilizer), DSCB-A1 ($200\;ml{\cdot}m^{-2}$DSCB-A), OSCB-A2 ($250\;ml{\cdot}m^{-2}$DSCB-A), OSCB-U ($250\;ml{\cdot}m^{-2}$DSCB-U) and CF+SCB (CF+$250\;ml{\cdot}m^{-2}$SCB). Every treatment was arranged in a randomized complete block design with three replications. In kentucky bluegrass, turf color index, chlorophyll index, dry weight and nutrient contents were measured. Results were as follows; It was hardly affected by DSCB and SCB application in investigation of chemical properties of soil. Turf color index and chlorophyll index in DSCB and SCB treatments were increased by 1~2% and 19~24% than NF, respectively and similar to CF. As applied with DSCB and SCB, dry weight of DSCB-U and CF+SCB was increased by 36% and 10% than CF, respectively, but similar to that of OSCB-A1 and DSCB-A2. Evaluated with turf quality and growth, DSCB-U was the best in all treatment and OSCB-A1 the most efficient. These results indicated that applications of OSCB and SCB promoted turf quality and growth of kentucky bluegrass or similar to CF, so that they were expected to replace chemical fertilizers.