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Uncoupling Protein 3 in the Rainbow Trout, Oncorhynchus mykiss Sequence, Splicing Variants, and Association with the AvaIII SINE element

  • Kim, Soon-Hag;Choi, Cheol-Young;Hwang, Joo-Yeon;Kim, Young-Youl;Park, Chan;Oh, Berm-Seok;Kimm, Ku-Chan;Scott A. Gahr;Sohn, Young-Chang
    • 한국양식학회지
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    • 제17권1호
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    • pp.1-7
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    • 2004
  • A rainbow trout uncoupling protein 3 (UCP3) cDNA clone, encoding a 310 amino acid protein, was cloned and sequenced from a liver cDNA library. Two different splice variants designated UCP3-vl and UCP3-v2, were identified through liver cDNA library screening using rainbow trout UCP3 cDNA clone as a probe. UCP3-vl has 3 insertions in the UCP3 cDNA: the first insertion (133 bp), the second (141 bp), and the third (370 bp) were located 126 bp, 334 bp and 532 bp downstream from the start codon, respectively. UCP3-v2 contained a single insertion, identical in sequence and location to the second insertion of UCP3-vl. UCP3, a mitochondrial protein, functions to modulate the efficiency of oxidative phosphorylation. UCP3 has been detected from heart, testis, spinal cord, eye, retina, colon, muscle, brown adipose tissue and white adipose tissue in mammalian animals. Human and rodent UCP3s are highly expressed in skeletal muscle and brown adipose tissue, while they show weak expression of UCP3 in heart and white adipose tissue. In contrast to mammalian studies, RT-PCR and Southern blot analysis of the rainbow trout demonstrated that UCP3 is strongly expressed in liver and heart. UCP3, UCP3-vl, and UCP3-v2 all contain an Ava III short interspersed element (SINE), located in the 3'untraslated region (UTR). PCR using primers from the Ava III SINE and the UCP3 3'UTR region indicates that the UCP3 cDNA is structurally conserved among salmonids and that these primers may be useful for salmonid species genotyping.

무지개송어, Oncorhynchus mykiss의 종묘생산에 관한 연구 - 미국산 무지개송어의 부화 및 자치어 성장 - (Studies on Seed Production of Rainbow Trout, Oncorhynchus mykiss - Hatching rate and early stage performance of USA strain rainbow trout, Oncorhynchus mykiss -)

  • 백국기;최영희;이중철;박인식;김영길;김두년;이채성
    • 한국양식학회지
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    • 제20권2호
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    • pp.85-89
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    • 2007
  • 무지개송어 품종개량의 일환으로 국내에 이식 양식되고 있는 미국산 무지개송어 발안난의 부화와 자치어 성장에서 얻어진 초기사육에 관한 기초적 시험을 실시하고 그 결과는 다음과 같다. 무지개송어 발안란의 난경은 $4.95{\pm}0.34\;mm$, 중량은 $0.07{\pm}0.003\;g$였다. 발안란은 8일째인 11월 25일에 전량 부화되었으며, 부화율은 평균 87.8%였다. 무지개송어 치어를 56일간 사육한 결과 전장 $4.0{\pm}0.7\;cm$로 매우 완만한 성장을 보였으며, 전장의 사육 경과일수에 따른 성장식은 TL=0.0366ED+1.8645 ($r^2=0.9285$, n=350)로 표시되었다. 부화된 치어의 평균 체중은 0.09 g였으며, 사육 $14{\sim}20$일째 $0.11{\sim}0.14\;g$로 서서히 증가되어 사육 41일째 0.33 g, 사육 48일째 0.45 g, 56일째 0.62 g로 급증되었으며, 체중의 사육 경과 일수에 따른 성장식은 $BW=0.0638e^{0.0408ED}\;(r^2=0.9917,\;n=350)$로 표시되었다. 치어의 56일간 사육 시 생존율은 $64.5{\pm}1.3%$였다.

Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

  • Yun, Hyeonho;Park, Gunjun;Ok, Imho;Katya, Kumar;Hung, Silas SO;Bai, Sungchul C.
    • Fisheries and Aquatic Sciences
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    • 제19권1호
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    • pp.4.1-4.7
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    • 2016
  • This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 % dry diet). A total of 35 fish averaging $512{\pm}6.8g$ ($mean{\pm}SD$) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of the experimental diets by intubation at 1 % body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free lysine concentrations (PPlys, 5 h after intubation) of fish fed diets containing ${\geq}2.32%$ lysine were higher than those of fish fed diets containing ${\leq}1.92%$ lysine. Post-absorptive free lysine concentrations (PAlys, 24 h after intubation) of fish fed diets containing 2.32 and 3.52 % lysine were higher than those of fish fed diets containing ${\leq}1.52%$ lysine. The brokenline regression analysis on the basis of PPlys and PAlys indicated that the lysine requirement of rainbow trout could be 2.34 and 2.20 % in diet. Therefore, these results strongly suggested that the dietary lysine requirement based on the broken-line model analyses of PPlys and PAlys could be greater than 2.20 % but less than 2.34 % (corresponding to be $6.01%{\leq},but{\leq}6.39%$ in dietary protein basis, respectively) in rainbow trout. Also, these results shown that the quantitative estimation of lysine requirement by using PPlys and PAlys could be an acceptable method in fish.

방사선(放射線) 조사(照射)에 의한 한국산(韓國産) 어류(魚類)의 품질보존(品質保存)에 관(關)한 연구(硏究) -제2보(第2報) 민물어종(魚種)(잉어 및 무지게 송어)의 방사선조사(放射線照射)- (Irradiation Preservation of Korean Fishes -Part. II Radurization of Freshwater Species-)

  • 정종락;김수일;이민철
    • 한국식품과학회지
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    • 제8권3호
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    • pp.161-171
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    • 1976
  • 잉어(carp, Cyprinus carpio, Linne)와 무지개송어(rainbow trout, Salmo grairdnerii) 육편을 알미늄-포리에치렌 봉지에 포장하여 1.5 Mrad까지의 감마선에 조사처리하여 냉장온도에서의 저장기간을 현저히 연장 시킬수 있는 최적선량을 조사하였다. 잉어의 최고 허용선량은 1.5 Mrad 그리고 무지개송어는 0.2 Mrad 이었으며 최적선량은 각각 0.25 Mrad와 0.05 Mrad이었다. 이 최적선량에 조사처리 하므로써 잉어의 저장기간은 $0^{\circ}C$$5^{\circ}C$에서 공히 1주에서 5주로 연장될 수 있었으며 무지개 송어는 $0^{\circ}C$에서는 1주에서 3-4주로, $5^{\circ}C$에서는 3일에서 14일로 각각 연장될 수 있었다. 조사후 저장기간중 잉어는 체액유출(drip loss)이 심했으므로 조사전에 육편을 10% 나토륨 포리인산염액에 침지 처리한 다음 조사한 결과 거의 완전히 방지할 수 있었다. 무지개송어는 방사선조사에 대단히 민감하였으나 잉어는 조사후 냉장온도에서의 저장성 연장을 목적으로 하는 저선량 조사처리에 매우 적합한 어종임이 규명되었다.

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MAD/MAM을 이용한 치과용 지르코니아 코어의 변연 적합도 (Marginal fidelity of zirconia core using MAD/MAM system)

  • 강동림;심준성;문홍석;이근우
    • 대한치과보철학회지
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    • 제48권1호
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    • pp.1-7
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    • 2010
  • 연구 목적: 본 연구에서는MAD/MAM 시스템으로 제작된 지르코니아 코어를 통상적인 방법으로 제작된 금속-도재관 코어와 CAD/CAM 시스템으로 제작된 코어와의 변연부 적합도를 비교 분석해 보고자 한다. 연구 재료 및 방법: 레진치를 전부 도재관 제작을 위한 형태로 삭제하고, 이를 복제하여 석고 모형을 제작하였다. 석고 모형에 대해 금속-도재관, CAD/CAM ($Cercon^{(R)}$), 3종의 MAD/MAM 시스템 ($Ceramill^{(R)}$, $Rainbow^{(TM)}$, $Zirkonzhan^{(R)}$)으로 각각 10개의 코어를 제작하였다. 제작된 코어를 다이에 합착하여 광학 현미경 상에서 50배율로 변연 간격을 관찰, 계측하였다. 측정된 결과는 one-way ANOVA test로 분석하고, Tukey's HSD test로 사후검정 하였다. 결과: 각 시스템에서 변연 간격의 평균과 표준 편차는 금속-도재관 코어 $51.97{\pm}23.38{\mu}m$, $Cercon^{(R)}$ $62.16{\pm}25.88{\mu}m$, $Ceramill^{(R)}$ $67.64{\pm}40.38{\mu}m$, $Rainbow^{TM}$ $125.07{\pm}42.19{\mu}m$, $Zirkonzhan^{(R)}$ $105.02{\pm}44.61{\mu}m$로 측정되었다. 결론: 금속-도재관 코어나 CAD/CAM에 비해 MAD/MAM 방식으로 제작된 코어의 변연 간격이 큰 것으로 나타났다 (P < .05). MAD/MAM 시스템 내에서도 $Ceramill^{(R)}$, $Zirkonzhan^{(R)}$, $Rainbow^{TM}$ 순으로 변연 간격이 작은 것으로 측정되었으며, 이중 $Ceramill^{(R)}$은 CAD/CAM 시스템인 $Cercon^{(R)}$과 변연 간격에 유의차가 없었다.

무지개송어, 은연어 및 그 잡종3배체의 체형 비교 (Morphometric Comparison in Rainbow Trout, Oncorhynchus mykiss, Coho Salmon, Oncorhynchus kisutch, and their Allotriploid)

  • 홍경표;김병기;명정구;김종만;허형택
    • 한국수산과학회지
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    • 제30권4호
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    • pp.687-689
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    • 1997
  • For identification of morphometric characteristics in rainbow trout, Oncorhynchus mykiss, coho salmon, O. kisutch, and their allotriploid, twenty one body traits were measured, and the ratios of each trait to fork length, length from postorbital to the origin of caudal fin and head length were calculated. The ratios of fin length to fin height in anal, dorsal and adipose fins showed significant differences among these two species and their allotriploid $(p\leq0.01)$. Allotriploid could be distinguished easily from two parents species in the ratios of length of origin of ventral fin to fork length and anal fin length to anal fin height.

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Membrane Hyperpolarization Increases cAMP to Induce the Initiation of Sperm Motility in Salmonid Fishes, Rainbow Trout and Masu Salmon

  • Kho, Kang-Hee;Morisawa, Masaaki;Choi, Kap-Seong
    • Journal of Microbiology and Biotechnology
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    • 제13권6호
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    • pp.833-840
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    • 2003
  • Sperm motility of salmonid fishes is suppressed by external $K^{+}$ and initiated by decrease of $K^{+}$ concentration surrounding the sperm. It was shown that the decrease in external $K^{+}$ concentration induced not only the initiation of sperm motility, but also hyperpolarization of the plasma membrane and synthesis of cAMP in the sperm of rainbow trout, steelhead trout, and masu salmon. Inhibitors of $K^{+}$ channels, especially voltage-dependent $K^{+}$ channels, inhibited these three reactions, and the inhibitions were abolished by subsequent addition of a $K^{+}$ ionophore, valinomycin, suggesting that $K^{+}$ efflux through the $K^{+}$ channel contributes to rapid changes in the membrane potential of sperm and cAMP synthesis, thereby resulting in the initiation of sperm motility of salmonid fishes.

Manufacture of Rainbow-colored Veneer by Natural Dyeing

  • ;박령재
    • 한국가구학회지
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    • 제26권3호
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    • pp.286-290
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    • 2015
  • The wood veneers were clearly rainbow-colored with natural dyes. As shown through Korean-style jacket with stripes of multi-colors beyond traditional obang colors (red, blue, yellow, black and white colors), eco-friendly coloring methods representing Korean colors familiar from old times could be used nobly by coloring natural wood veneer being raw material of wood products. In terms of industrialization, the study to manifest korean color, substituting chemical stains such as dye and pigment, would be necessary. In order to realize this purpose, the study about economical dyeing materials and characteristics, that is, mordant, dyeing and drying techniques showing environment-friendly coloring and high coloration level ought to be followed. In addition to this, investigating discoloration transition by fading test for interior and exterior uses would have to be carried out.