• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.595-598
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• 2000

Aeromonas hydrophila is a pathogen to fish as well as human. It is a food-borne disease, and causes severe mortality in fish, and sometimes severe septicemia in human. In this study, a rapid detection method using latex agglutination has been developed for A. hydrophila. Polyclonal antibodies were raised against membrane and whole cells of three isolates from rainbow trout. Among these, latex particles coated with antibodies raised against whole cells of isolate No. 2 showed the best sensitivity. With latex particles coated with this antibody, we could detect $5{\times}10^4$ CFU of A. hydrophila in 5 min. The cross-reactivity with bacteria constituting the normal intestinal microflora and other pathogens for rainbow trout was insignificant. This latex agglutination assay method produced positive reaction with all clinical isolates of A. hydrophila which were identified by species-specific PCR for 16S rRNA in A. hydrophila.

• Journal of the Korean Institute of Landscape Architecture International Edition
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• no.1
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• pp.202-207
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• 2001

Covering an area of about 39 ha, Qingming Shanghe Park is located in the northwest of Kaifeng, formerly the capital of seven dynasties in China. It is intended to serve as a tourist attraction based on a painting 〃Qingming Festival at Riverside〃 by Zhang Zeduan of the Northern song Dynasty, which vividly illustrates a real life on the both sides of the Bian River as well as in the urban area, Kaifeng, in early Spring. The park is a collection of varied buildings such as shops, restaurants, tea-houses, theaters, and hotels in the North song style, with an introduction of the history of Kaifeng, this paper examines design philosophy and methodology of the park, and discusses the creative design of the first stage development known as known as The South Area of the park. Marked by the Rainbow Bridge and the City Gate, the South Area is divided into three parts, the rural, suburban, and urban parts which are organized according to the spatial order showed in the painting. The South Area proves to be very successful because of its amazing sense of history and interesting folk performance. Furthermore, the preliminary 9planning of the second stage development known as The North Area is presented with references to culture, history and customs. The North Area is proposed to include the Old Sports & Games Garden, the Exhibition Garden, and the Hot Spring Garden with an appearance of the imperial gardens in the Northern Song, integrated with participation of old sports and games, display of old science & technology achievements, and enjoyment of hot spring bath.

• Proceedings of the Korea Information Processing Society Conference
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• 2018.10a
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• pp.204-207
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• 2018

IoT 기기 및 드론의 사용자 인증 및 기기 인증을 위해 RSA, ECDSA 등의 여러 전자서명 기법이 기본적으로 사용되고 있다. 그러나 양자 컴퓨터의 개발에 따라 Shor 알고리즘을 이용한 기존 암호 알고리즘의 공격이 가능해지고, 그에 따라 기존 암호 알고리즘의 보안성이 취약해지는 문제가 있다. 따라서 양자 내성 암호를 활용한 보안 체계의 필요성이 대두되고 있는 가운데, 본 논문에서는 양자 내성 암호인 다변수 이차식 기반의 전자서명 기법 중 Rainbow를 드론에 최적화하여 구현하기 위한 방안을 검토 및 분석하고자 한다. 그러나 기존의 Rainbow에서 사용하는 openssl 등의 오픈소스 암호 라이브러리는 PC에 맞춰 설계되었기 때문에 드론에서 난수를 생성할 때 적용이 어려운 점이 있다. 드론에는 각종 센서들이 내장되어 있으며, 센서 데이터들은 난수성을 보장하기에 용이하다. 따라서 드론의 각종 센서들을 시드로 활용하며, XOR 보정기를 통해 난수성을 해치지 않으면서 드론에서 난수를 생성할 수 있는 방안을 제안해 보고자 한다.

• Journal of fish pathology
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• v.25 no.3
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• pp.143-150
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• 2012

Infectious haematopoietic necrosis virus (IHNV) is an important fish pathogen that infects both wild and cultured salmonids. Since the first isolation of IHNV from rainbow trout and masu salmon in 1991, a series of IHN disease outbreak has been reported in Korea. In 2011, we isolated two IHNV isolates from rainbow trout cultured in Korea. The full open-reading frame (ORF) encoding the glycoprotein (G) of them were sequenced and the amino acid sequences were phylogenetically analyzed. Phylogenetic analysis of the G revealed that both IHNV isolates were grouped into an Asian genogroup containing Korean IHNV isolates and Japanese IHNV isolates. However, based on their sequence variation, they were divided into different subgroup. While one isolate was similar to other Korean isolates, the other isolate showed a high level of similarity with Japanese isolates, suggesting the possibility of influx of new IHNV strain into Korea.

• Journal of Ceramic Processing Research
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• v.19 no.6
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• pp.479-482
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• 2018

Polystyrene superstructure films show faint rainbow color, and this low color saturation limits its wide application. In this paper, polystyrene superstructure films with single bright blue color were prepared by vertical deposition self-assembly method using polystyrene microspheres with average diameter of $310{\pm}10nm$ as raw material. Polystyrene superstructure films were modified by adding nano-carbon powder, and effect of the amount of nano-carbon powde on color performance was studied. The results showed that without addition of nano-carbon powder, the superstructure films showed a faint rainbow color, while with addition of nano-carbon power, the superstructure films exhibited a single bright blue under the same natural light source. Changing the amount of nano-carbon powder addition could adjust color saturation of the film. With increasing the amount of nano-carbon powder addition from 0.008 wt% to 0.01 wt%, color saturation of the superstructure film increased gradually. Further increasing the amount of nano-carbon powder addition to 0.011wt%, color saturation of the superstructure film didn't increase anymore and tended to get dark.

• Journal of Marine Life Science
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• v.8 no.2
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• pp.186-189
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• 2023

Uncoupling protein 1 (UCP1) is a unique mitochondrial membranous protein expressed in brown adipose tissue (BAT) in mammals. While its expression in response to cold temperatures and adipogenic inducers is well-characterized in mammals and human infants, the molecular characterization and expression of UCP1 in fish remain unexplored. To address this gap, we analyzed UCP1 expression in response to adipogenic inducers in a fish cell line, rainbow trout gonadal cells (RTG-2), and compared it with UCP1 expression in three mammalian preadipocytes, 3T3-L1, T37i, and WT1 exposed to the Peroxisome proliferator-activated receptor gamma (PPARγ) agonists, rosiglitazone (Rosi). In mammalian preadipocytes, UCP1 protein was highly expressed by Rosi, with an induction of adipogenesis observed in a time-dependent manner. This suggests that UCP1 plays a significant role in adipogenesis in mammals. However, RTG-2 cells showed no response to adipogenic inducers and exhibited only marginal expressions of UCP1. These results imply that RTG-2 cells may lack crucial responsive mechanisms to adipogenic signals or that the adipogenic response is regulated by other mechanisms. Further studies are needed to confirm these phenomena in fish preadipocytes when an appropriate cell line is established in future research.

• Journal of fish pathology
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• v.11 no.1
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• pp.51-60
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• 1998

Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature: $18{\pm}1^{\circ}C$ DO: 7-7.5 ppm; pH: $7{\pm}0.2$). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at $18^{\circ}C$. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at $18^{\circ}C$. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at $18^{\circ}C$ would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from $54{\mu}m$ on the 1st day PE to $426{\mu}m$ on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.3
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• pp.348-356
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• 2008

For the effective use of rainbow trout, the seasoned and dried rainbow trout slice (SR) was prepared and its characterization was compared with the commercial skipjack tuna (CSS) and pork products (CSP) by determining chemical components and sensory evaluation. The moisture content of SR, 20.5%, was lower, and the lipid content of SR, 7.5%, was higher than those of commercial products, CSS and CSP. The protein content of SR (41.4%) was higher than that of CSS (28.6%), but lower than that of CSP (50.1%). The water activity of SR was 0.654, which was lower than those of CSS and CSP, 0.724 and 0.771, respectively. According to the results, the color of SR was lower in lightness and redness and higher in browning index and ${\Delta}$E value than that of commercial products. The texture of SR was harder than that of CSS, but softer than that of CSP. According to the result of taste value, the taste of SR was stronger than that of CSP, while weaker than that of CSS. The mineral (Ca, P, and Fe), total amino acid contents, and the n-3/n-6 of SR showed significant levels in nutrition and health functional senses.

• Journal of Aquaculture
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• v.9 no.3
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• pp.265-278
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• 1996

A long-term (160 days) feeding experiment was conducted to evaluate effects of dietary soybean meal (SBM) on growth of rainbow trout (Oncorhynchus mykiss). Fish fed diets containing up to $46\%$ of SBM showed the same growth rates with control during the first 60 days (p>0.05). However, growth showed a gradual decrease with the culture period. At the end of 160-days experiment, fish fed diets with above $34\%$ of SBM showed a decrease in growth rate compared to the control group. Fatness of experimental fish was reduced with an increase in dietary SBM levels and in feeding period. Hepatosomatic index was significantly lower from fish fed the diets containing above $46\%$ of SBM than that from fish fed the control diet throughout the 160 days of experimental period, and fish fed diet containing above $46\%$ of SBM appeared to make restricted utilization of carbohydrate sources. These results may suggest that soybean meal could be used up to $22\%$ in trout diet as a substitute for fish meal without unfavourable side effects.

• Analytical Science and Technology
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• v.24 no.6
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• pp.544-555
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• 2011

The acute toxicity in the rainbow trout (Oncorhynchus mykiss) was analyzed and predicted using quantitative structure-activity relationships (QSAR). The aquatic toxicity, 96h $LC_{50}$ (median lethal concentration) of 275 organic pesticides, was obtained from EU-funded project DEMETRA. Prediction models were derived from 558 2D molecular descriptors, calculated in PreADMET. The linear (multiple linear regression) and nonlinear (support vector machine and artificial neural network) learning methods were optimized by taking into account the statistical parameters between the experimental and predicted p$LC_{50}$. After preprocessing, population based forward selection were used to select the best subsets of descriptors in the learning methods including 5-fold cross-validation procedure. The support vector machine model was used as the best model ($R^2_{CV}$=0.677, RMSECV=0.887, MSECV=0.674) and also correctly classified 87% for the training set according to EU regulation criteria. The MLR model could describe the structural characteristics of toxic chemicals and interaction with lipid membrane of fish. All the developed models were validated by 5 fold cross-validation and Y-scrambling test.