• Title/Summary/Keyword: race identification

Search Result 54, Processing Time 0.023 seconds

Root-knot Nematode Species Distributing in Greenhouses and Their Simple Identification Scheme (시설원예지에 분포하는 뿌리혹선충의 종류 및 간이 동정법)

  • 김동근;이영기;박병용
    • Research in Plant Disease
    • /
    • v.7 no.1
    • /
    • pp.49-55
    • /
    • 2001
  • Species and races of root-knot nematodes in greenhouses in southern Korea were investigated and a simple identification scheme was provided. Among 23 populations of root-knot nematodes, Meloidogyne arenaria race 2 was 59%, M. incognita race 1 was 23%, and an unknown race of M. incognita was 18%. Total length of M. arenaria juveniles was 411㎛(306-503㎛) and that of M. incognita was 384㎛(312-488㎛); however, the ranges of two species were overlapped and could not be used to distinguish the two species. Excretory pore in female head was a consistent character to differentiate M. arenaria and M. incognita.

  • PDF

Pathogenic Diversity of Ascochyta rabiei Isolates and Identification of Resistance Sources in Core Collection of Chickpea Germplasm

  • Farahani, Somayeh;Talebi, Reza;Maleki, Mojdeh;Mehrabi, Rahim;Kanouni, Homayoun
    • The Plant Pathology Journal
    • /
    • v.35 no.4
    • /
    • pp.321-329
    • /
    • 2019
  • Ascochyta blight caused by Ascochyta rabiei (Pass.) Lab. (Telomorph: Didymella rabiei) (Kov.) is one of the most important fungal diseases in chickpea worldwide. Knowledge about pathogen aggressiveness and identification resistance sources to different pathotypes is very useful for proper decisions in breeding programs. In this study, virulence of 32 A. rabiei isolates from different part of Iran were analyzed on seven chickpea differentials and grouped into six races based on 0-9 rating scale and susceptibility/resistant pattern of chickpea differentials. The least and most frequent races were race V and race I, respectively. Race V and VI showed highly virulence on most of differential, while race I showed least aggressiveness. Resistance pattern of 165 chickpea genotypes also were tested against six different A. rabiei races. ANOVA analysis showed high significant difference for isolate, chickpea genotypes and their interactions. Overall $chickpea{\times}isolate$ (race) interactions, 259 resistance responses (disease severity ${\leq}4$) were identified. Resistance spectra of chickpea genotypes showed more resistance rate to race I (49.70%) and race III (35.15%), while there were no resistance genotypes to race VI. Cluster analysis based on disease severity rate, grouped chickpea genotypes into four distinct clusters. Interactions between isolates or races used in this study, showed the lack of a genotype with complete resistance. Our finding for virulence pattern of A. rabiei and newly identified resistance sources could be considerably important for integration of ascochyta blight resistance genes into chickpea breeding programs and proper decision in future for germplasm conservation and diseases management.

Distribution of Root-knot Nematodes, Meloidogyne spp. and Their Races in Economic Crops in Korea (주요 경제작물에 기생하는 뿌리혹 선충의 종과 Race 분포)

  • Cho H. J.;Kim C. H.;Park J. S.;Jeoung M. G.
    • Korean Journal Plant Pathology
    • /
    • v.3 no.3
    • /
    • pp.159-163
    • /
    • 1987
  • In order to investigate the distribution and density of root-knot nematodes in economic crops, samples were collected from 3,226 fields of 18 economic crops. Nematodes were extracted using a modified Baermann's funnel technique. Identification of races was based on the differential host-test for Meloidogyne spp. by Tayler and Sasser. M. hapla was dominant in fields in the middle parts of Korean peninsula; M. incognita, in the southern parts, and distribution of M. javanica was limited to Jeju island and southern seaside areas. Two races of M. arenaria identified in this study were races 1 and 2. The three races identified in M. incognita were races, 1, 2 and 3. Of these races, race 1 of M. arenaria and race 3 of M. icognita were identified for the first time in Korea.

  • PDF

Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 5 (콩 Cyst 선충 Race 5에 대한 저항성 QTL 탐색)

  • Choi, In-Soo;Kim, Yong-Chul;Kim, Sung-Man;Lee, Chung-Yeol;Park, Hyean-Cheal;Halina T. Skorupska
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.42 no.6
    • /
    • pp.712-721
    • /
    • 1997
  • The objectives of this study were; (1) to identify and localize QTLs for resistance to soybean cyst nematode(SCN) race 5 on RAPD map, (2) to idntify the magnitude and mode of inheritance for each QTL, and (3) to identify the best combinations of QTLs for resistance to SCN race 5. Based on the univariate regression analysis, we detected 26 markers(22 RAPD and 4 RFLP) which showed significant association(P<0.05) with resistance to SCN race 5. From MAPMAKER /QTL analysis, we identified two regions (LGC-20 and Group 2) for resistance to SCN race 5. The QTL that was localized at 8.0 cM from pK418C on LGC-20 showed a recessive mode of inheritance and the QTL that was localized between W03 and E02$^3$ on Group 2 showed a dominant mode of inheritance. Two pairs of flanking markers (E02$^3$ and W03, pK418C and pK418E$_1$) and one unlinked RAPD marker, G10$^1$ were used for multiple regression analysis. Marker combination which was composed of 4 markers, E02$^3$, G10$^1$, W03, and pK418E$_1$, explained the highest amount of phenotypic variation by SCN (35.2%). Further research for the identification of QTLs for resistance to SCN race 5 to explain larger portion of phenotypic variation is needed.

  • PDF

Identification of fungal races that cause powdery mildew in melon (Cucumis melo L.) and selection of resistant commercial melon cultivars against the identified races in Korea (국내 멜론 흰가루병균의 race 동정 및 시판품종의 흰가루병 저항성 판별)

  • Kim, Hoy-taek;Park, Jong-in;Nou, Ill-sup
    • Journal of Plant Biotechnology
    • /
    • v.43 no.1
    • /
    • pp.58-65
    • /
    • 2016
  • Powdery mildew is an important disease of the melon (Cucumis melo L.). Seven isolates of powdery mildew fungi were collected from five locations in Korea; Anseong (DH487), Icheon (BN103, BN625, BN968), Yeongam (YA141), Changnyeong (CN582), and Suncheon (SN102). All 7 fungi had a similar trend of conidial chain and conidiophore development as Podosphaera xanthii with fibrosin bodies in mature conidia. Among them, 2 isolates of powdery mildew fungi; CN582 and SN102 showed similar responses to resistance against powdery mildew as the previously reported race 1 and race N2. The isolates YA141 and BN103 showed similar responses as like as race A. However, three isolates of powdery mildew fungi (BN625, BN968, and DH487) showed different responses compared to the previously reported races (1, N1, N2, A, S, and 5). Therefore, these three isolates could be designated as new races in melon. Nine out of 15 commercial melon cultivars in Korea showed resistance to race 1 (CN582). However, the new race BN968 invaded all 15 cultivars. Results of the two molecular markers were consistent in response to disease development by race 1 of Podosphaera xanthii in case of the above mentioned cultivars. This study confirmed the presence of new melon powdery mildew fungi in Korea which are similarly notorious as like as the previously reported race 1. Therefore, breeders can use these two molecular markers for breeding melon in Korea that is resistant to race 1 and as well as to some other races.

Identification of a host range determinant from Ralstonia solancearum race 3

  • Yeonhwa Jeong;Lee, Seungdon;Ingyu Hwang
    • Proceedings of the Korean Society of Plant Pathology Conference
    • /
    • 2003.10a
    • /
    • pp.71.2-71
    • /
    • 2003
  • Ralstonia solancearum infects many solanaceous plants, however race 3 infects only potato and tomato weakly. To identify genes responsible for race specificity of R. solanacearum, we mobilized genomic library of LSD2029 (race 3) into LSD341 (race 1) and inoculated 1,000 transconjugants into hot pepper. One transconjugant that did not induce wilt symptom in hot pepper was isolated. We found that a cosmid clone, pRSl, conferred avirulence to LSD341. By deletion and mutational analyses of pRSl, we found the 0.9-kb PstI/Hindlll fragment carries avirulence functions. We sequenced the fragment and identified one possible open reading frame, a rsal gene, possibly encoding 110 amino acids. The rsal was preceded with a plant-inducible promoter (PIP) box, indicating that the gene might be regulated by HrpB. Interestingly, the promoter region of the rsal homolog in the strain GM11000 (race 1) did not have the PIP box. Rsal did not show any significant homologies with proteins in the database, indicating th e protein is different from the previously reported avirulence proteins. When we mutated the rsal gene by marker-exchange in LSD2029, the mutant was less virulent in potato.

  • PDF

Defects Diagnosis of Ball Bearings by Neural Network (신경회로망을 이용한 볼 베어링의 결함진단)

  • 양보석;최성필;최원호;김진욱
    • Journal of Advanced Marine Engineering and Technology
    • /
    • v.18 no.5
    • /
    • pp.36-45
    • /
    • 1994
  • This paper describes how to identify standard numbers and to diagnose defects of the ball bearings. The first stage of the networks is a procedures for identifying standard numbers of the bearings, and the next stage carries out the diagnosis of defects on the outer race and the inner race of bearings. The identification and the diagnosis of bearings were carried out by simulations and experiments.

  • PDF

PCR-Based Assay for Rapid and Specific Detection of the New Xanthomonas oryzae pv. oryzae K3a Race Using an AFLP-Derived Marker

  • Song, Eun-Sung;Kim, Song-Yi;Noh, Tae-Hwan;Cho, Heejung;Chae, Soo-Cheon;Lee, Byoung-Moo
    • Journal of Microbiology and Biotechnology
    • /
    • v.24 no.6
    • /
    • pp.732-739
    • /
    • 2014
  • We describe the development of a polymerase chain reaction method for the rapid, precise, and specific detection of the Xanthomonas oryzae pv. oryzae (Xoo) K3a race, the bacterial blight pathogen of rice. The specific primer set was designed to amplify a genomic locus derived from an amplified fragment length polymorphism specific for the K3a race. The 1,024 bp amplicon was generated from the DNA of 13 isolates of Xoo K3a races out of 119 isolates of other races, pathovars, and Xanthomonas species. The assay does not require isolated bacterial cells or DNA extraction. Moreover, the pathogen was quickly detected in rice leaf 2 days after inoculation with bacteria and at a distance of 8 cm from the rice leaf 5 days later. The results suggest that this PCR-based assay will be a useful and powerful tool for the detection and identification of the Xoo K3a race in rice plants as well as for early diagnosis of infection in paddy fields.

The Study of Human Body Expression and Fashion Design Appeared in Popular Culture -Focused on Movies in Korea and American- (대중문화에서 보여지는 신체표현과 복식디자인 연구 - 미국과 국내 영화를 중심으로 -)

  • 권기영
    • The Research Journal of the Costume Culture
    • /
    • v.12 no.4
    • /
    • pp.676-690
    • /
    • 2004
  • The purpose of this study is to investigate the symbolic meaning in body image and fashion style portrayed in Korea and American movies in aspects of sex, race, nature, and technology. The researcher analyzed movies in Korea and USA, and the results were as follows. In aspects of sex, movies portrayed new masculinity and femininity escaped from previous gender stereotypes, and the clothing styles characterized sex identity and sex roles. In aspects of race, more various races appeared in America movies than Korea's, and the appearance and stereotypes related with race were blurred by the globalization and the change of film market environments. In aspects of nature, Korea movie portrayed the human being as lyrical appearance in nature, and America movie expressed the human as a strong men conquering the nature in adventure movie or super human image combined with nature thing which endowed nature's superior characteristics to human body in fantasy and science fiction movies. In aspects of technology, the human bodies were described as cyborg, alien, or super human, which symbolized the do-identification in digital world, de-boundarization between human and machines, and the human's uncertainty.

  • PDF

Identification of Two New Races of Podosphaera xanthii Causing Powdery Mildew in Melon in South Korea

  • Hong, Ye-Ji;Hossain, Mohammad Rashed;Kim, Hoy-Taek;Park, Jong-In;Nou, Ill-Sup
    • The Plant Pathology Journal
    • /
    • v.34 no.3
    • /
    • pp.182-190
    • /
    • 2018
  • Powdery mildew caused by the obligate biotrophic fungus Podosphaera xanthii poses a serious threat to melon (Cucumis melo L.) production worldwide. Frequent occurrences of the disease in different regions of South Korea hints at the potential existence of several races which need to be identified. The races of five isolates collected from different powdery mildew affected regions were identified based on the pathogenicity tests of these isolates on eight known differential melon cultigens namely, SCNU1154, PMR 45, WMR 29, PMR 5, MR-1, PI124112, Edisto 47 and PI414723. None of the isolates have shown same disease responses to those of the known races tested in this study and in previous reports on these identical differential melon cultigens. This indicates that the tested uncharacterized isolates are new races. Among the isolates, the isolates from Hadong, Buyeo, Yeongam and Gokseong have shown same pathogenicity indicating the possibility of these isolates being one new race, for which we propose the name 'race KN1'. The isolate of Janghueng have also shown unique disease response in the tested differential melon cultigens and hence, we identified it as another new race with a proposed name 'race KN2'. Report of these new races will be helpful in taking effective control measures in prevalent regions and for future breeding programs aimed at developing varieties that are resistant to these race(s).