• Journal of the Korean Geotechnical Society
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• v.40 no.2
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• pp.29-40
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• 2024

This study aimed to evaluate the swelling behavior characteristics of KJ-II buffer blocks by performing numerical analysis of swelling pressure measurement experiments using the nonlinear elasticity model of COMSOL Multiphysics. The analysis was conducted under boundary conditions that included isotropic constraints and water injection pressure, mirroring the experimental settings. Validation of the numerical model was achieved by comparing its outputs with experimental results. The validated model was then used to simulate swelling deformations under unconfined conditions and to analyze swelling pressure as influenced by dry density and the geometric shape of the buffer material. The results accurately represented the swelling deformation observed during the saturation process and demonstrated that swelling pressure increases with higher dry density. Moreover, simulations concerning the geometric shape of the buffer material indicated a markedly faster rate of pressure increase in U-shaped samples compared to cylindrical ones. Analysis suggested that stress manifested preemptively near the internal edges of U-shaped samples during saturation. To enhance the simulation's fidelity to actual buffer material behavior, further refinement of the analysis model using a nonlinear elasticity model is recommended.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.10
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• pp.1550-1557
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• 2018

Objective: Circular RNAs (circRNAs) are a newfound class of non-coding RNA in animals and plants. Recent studies have revealed that circRNAs play important roles in cell proliferation, differentiation, autophagy and apoptosis during development. However, there are few reports about muscle development-related circRNAs in livestock. Methods: RNA sequencing analysis was employed to identify and annotate circRNAs from longissimus dorsi of sheep. Reverse transcription followed by real-time quantitative (q) polymerase chain reaction (PCR) analysis verified the presence of these circRNAs. Targetscan7.0 and miRanda were used to analyse the interaction of circRNA-microRNA (miRNA). To investigate the function of circRNAs, an experiment was conducted to perform enrichment analysis hosting genes of circRNAs using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: About 75.5 million sequences were obtained from RNA libraries of sheep skeletal muscle. These sequences were mapped to 729 genes in the sheep reference genome. We identified 886 circRNAs, including numerous circular intronic RNAs and exonic circRNAs. Reverse transcription PCR (RT-PCR) and DNA sequencing analysis confirmed the presence of several circRNAs. Real-Time RT-PCR analysis exhibited resistance of sheep circRNAs to RNase R digestion. We found that many circRNAs interacted with muscle-specific miRNAs involved in growth and development of muscle, especially circ776. The GO and KEGG enrichment analysis showed that hosting genes of circRNAs was involved in muscle cell development and signaling pathway. Conclusion: The study provides comprehensive expression profiles of circRNAs in sheep skeletal muscle. Our study offers a large number of circRNAs to facilitate a better understanding of their roles in muscle growth. Meanwhile, we suggested that circ776 could be analyzed in future study.

• Journal of Sasang Constitutional Medicine
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• v.4 no.1
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• pp.81-106
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• 1992

The purpose of this study was to find out the causal relationship between perceptual level of stress and health state according to Korean constitutions and to analyze that effect on the Korean constitution's health state. Subjects of this study were 87 out patient department of constitution at Kyung-Hee Oriental Medicine Hospital. Data were collected by using interview with Questionaire during 6 months from Feb. 2 to June 31, 1991. The Measurement tools used by this researcher were Go's The Questionaire of Identify about constitution, Lee's stress scale and Go's modified Cornell Medical Index which were approved it's reliability and validity. All of the questionaires of were used after pre- test. For the purposes of the study, the collected data were analyzed by frequency, t-test, ANOVA and Pearson correlation. And then this research's issue was tested by SAS program's ANCOVA. The findings of this study was summarized as follow. 1. There was significantly differences in stress perceptual level in Korean constitution. (F=9.68, P=.000). The So Em In (小陰人) were higher than the other constitution on stress perceptional level. 2. In health state, there was significantly difference among Korean constitution. (F=6.654, P=.002). Also the So Em In (小陰人) was lower than the other constitution at health state. Therefore, it was considerate that So Em In (小陰人) was higher perceptual level of stress than the other constitution and the resulting poor health state. 3. Stressful perception was effected on the health state, as a result inverted correlation was constructed between stress perception and health state. (r=-.6034, P=.0001) 4. After exclusion of stress perceptual level, each of the Korean Constitution's health state was not difference (F=1.01, P=.37). It was mean that differences of the Korean Constitution's perceptual level of stress effected on the health state. Bacause So Em In (小陰人) had higher perceptual level of stress than other constitution (F=9.687, P=.000), in proportion to that one's health state was poor. From the results of this study, it could be concluded that the higher stress perceptual level according to Korean constitution, the poorer health state. Further study in this area needs to be consideration that in order to coping with stress, researcher grope for quality nursing intervention with more accurative assessment about Korean constitution.

• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.233-245
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• 2013

Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an important source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differentiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphorylation, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.

• PNF and Movement
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• v.13 no.4
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• pp.181-188
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• 2015

Purpose: This study aimed to examine the effects of proprioceptive neuromuscular facilitation (PNF) lower extremity patterns combined with elastic bands applied to stroke patients diagnosed with hemiplegia through self-training using the non-paralyzed side approach and the paralyzed side approach, and to investigate the differences in the effects. Methods: Nine chronic stroke patients who were being treated not more than twice a week at H, K, R, and C hospitals located in Gangwon-do, performed self-training for 16 minutes, two times per day for four weeks between August and October 2015. The subjects' balance ability was measured using the Tetrax stability index, the weight distribution index, the Berg Balance Scale (BBS), and the Timed Up and Go (TUG) test before the experiment and four weeks later. Among the statistical methods, paired t-tests were conducted for intra-group comparison of the measurements taken before and after the experiment, and independent t-tests were conducted for inter-group comparison of the ex post facto values. The statistical significance level was set to 0.05. Results: When the lower extremity patterns were applied to the non-paralyzed side group and the paralyzed side group, significant intra-group differences were observed for the Tetrax stability index, the weight distribution index, the Berg Balance Scale (BBS), and Timed Up and Go (TUG) tests (p<0.05); however, the inter-group comparisons showed no significant differences. Conclusion: The non-paralyzed side approach was found to be easy for patients to participate in and it also affected the patients' paralyzed side. Although the paralyzed side approach produced good exercise effects in a short period of time, it could result in adverse effects, such as a decrease in motivation and self-confidence. Therefore, these approaches are considered to be more effective when they are selectively applied depending on the purpose of the intervention and the degree of a patients' participation.

• Animal Bioscience
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• v.36 no.3
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• pp.404-416
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• 2023

Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.924-932
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• 2013

This study investigated the antioxidant activities of processed Deoduck (Codonopsis lanceolata) extracts treated through high-pressure extraction and steaming with fermentation. The antioxidant activities were determined for DPPH and ABTS radical-scavenging activity, SOD-like activity, ferric reducing antioxidant power (FRAP), and $Fe^{2+}$ chelating. Total phenolic and flavonoid contents were also measured. Among eight Deoduck extracts, the S5FDW extract had the highest total phenolic and flavonoid content, 73.9 mg GAE/g and 50.9 mg QUE/g, respectively. The S5FDW extract had the highest DPPH radical-scavenging activity (27%) at a 1.0 mg/mL concentration. The ABTS radical-scavenging activity was highest for S5FDW extract (82.1%) at a 10 mg/mL concentration. The HFDE extract showed the highest SOD-like activity (29.7%) at a 1.0 mg/mL concentration. FRAP was highest in S5FDW extract (140.8 ${\mu}M$) at a 1.0 mg/mL concentration. The DE extract showed the highest $Fe^{2+}$ chelating (46%) at a 1.0 mg/mL concentration. The phenolic and flavonoid contents significantly correlated with the antioxidant activity of several processed Deoduck extracts and was higher in the processed Deoduck extracts compared to the raw Deoduck extracts. Therefore, processing techniques can be useful methods for making Deoduck a more potent and natural antioxidant.

• Child Health Nursing Research
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• v.8 no.1
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• pp.32-43
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• 2002

The purpose of this study was to provide basic data on elementary school children's lifestyle and to contribute to developing on the health education program in elementary schools. The subjects were 1,412 children in 4 elementary schools in Gangwon-Do and Chonrabuk-Do area. Data collection was done from September to November 2001 by questionnaire and school health documents. The questionnaire corrected for the purpose of this study which had been developed by Bronson School of Nursing(1991), 'Lifestyle Questionnaire for School-age Children'. The questionnaire consists of 3 categories; 'Activities that promote health', 'Injury prevention', 'Feeling'. Cronbach coefficient alpha for the 29 items was .68. The data analyzed to obtain frequency, mean, percentage, t-test, ANOVA and Pearson correlation coefficient by SPSS Win program. The results of this study were as follows. 1. Females(50.2%) of gender, 6th grade(24.2%) of grade, nuclear family(82.0%) of family type, beyond college graduate(54.5%) of father's school career, under high school graduate(58.1%) of mother's school career, first of birth order(47.1%) were majority. Mean of father's age was 41.2 and mother's age was 38.1. 2. The mean of lifestyle was 66.4, feeling was 73.3, activities that promote health was 60.3 and injury prevention was 64.0. The highest degree of activities that promote health was 「I eat fruits」and injury prevention was 「I look both ways when crossing streets」and feeling was 「I enjoy my family」. The lowest degree of activities that promote health was 「I visit the dentist every tear」 and injury prevention was 「I wear a helmet when I go on bike trips」 and feeling was 「I think it is okay to cry」. 3. There were significant differences in lifestyle of gender(t=4.309, p=.000), grade(F=6.299, p=.000), father's age(t=2.534, p=.011), father's education(t=-4.933, p=.000), mother's education(t=-3.360, p=.001), birth order (t=5.363, p=.000). There were significant differences in activities that promote health of gender(t=-2.462, P=.014), grade(F=4.893, p=.000), father's education(t=-4.480, p=.000), birth order(t=4.343, p=.000), in injury prevention of gender(t=-4.452, p=.000), grade(F=8.636, p=.000), father's age(t=3.386, p=.001), mother's age(t=2.059, p=.040), father's education(t=-6.051, p=.000), mother's education(t=-5.173, p=.000), birth order(t=4.417, p=.000) and in feeling of gender (t=-3.285, p=.001), grade(F=7.526, p=.000), mother's age(t=-3.268, p=.001). 4. Activities that promote health was positively correlated with injury prevention(r=.432, p=.000), feeling(r=.210, p=.000), lifestyle (r=.785, p=.000). Injury prevention was positively correlated with feeling(r=.256, p=.000), lifestyle(r=.854, p=.000) also feeling was positively correlated with lifestyle(r=.504, p=.000). These findings suggest the need to develop nursing strategy to promote elementary school children's health. Because helmet use score in injury prevention marked the lowest score, it is necessary to encourage helmet use when planning injury prevention and health promotion.

• Journal of Aquaculture
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• v.9 no.1
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• pp.43-55
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• 1996

The experiment was performed to evaluate the possibility of utilizing underground sea water for the seed production of tiger puffer, Takifugu rubripes. For this purpose, the effects of 6 different salinities (3.5, 7.0, 14.0, 20.0, 27.0, $33.0\%_{\circ}$) were determined based on the hatching rate of fertilized eggs, survival rate and the amount of food consumed by hatched larvae, where as the effects of 3 different salinities (20.0, 27.0, $33.0\%_{\circ}$) were also examined with rearing tiger puffer juvenile ($4.29{\pm}0.50$ cm in total length) for 50 days in the closed recirculating water system. As a results, either the hatching or the survival rate of more than $70.0\%$ were obtained from the fertilized eggs reared at the salinity of 27.0 to $33.0\%_{\circ}$, the early hatched larvae at 27.0 to $33.0\%_{\circ}$, and the 10-day-old larvae at 20 to $33.0\%_{\circ}$. At three different salinities, the survival rate of 20-day- and 30-day-old larvae turned out to be $89.0\%$ and $92.5\%$, respectively. The salinity for maximum food intake thus appeared to be from 27.0 to $33.0\%_{\circ}$. In this condition, 20-day-old hatched larvae consumed $323\~342$ Artemia nauplii and 30-day-old hatched larvae ate $1,559\~1,5791$ A. nauplii. The highest growth rate of fingerlings were observed at the salinity $27\%$ and followed by 33.0 and $20.0\%_{\circ}$, respectively. The relationship between the days of rearing (X) and the total length (Y) of the fingerlings were as follows : $33.0\%_{\circ}$ group : Y=0.107X-2.532 (r=0.982) $27.0\%_{\circ}$ group : Y=0.116X-3.195 (r=0.975) $20.0\%_{\circ}$ group : Y=0.116X-2.693 (r=0.987) The slopes of regression line estimated from $27.0\%_{\circ}$ and $33.0\%_{\circ}$ groups were significantly different from that of $20.0\%_{\circ}$ group.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.1
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• pp.59-71
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• 2012

In order to elucidate the mechanism of fishing condition variation of anchovy in the set net fisheries of Anggang Bay, the monthly catch of anchovy was analyzed and examined based on the data acquired from 2006 to 2010 in 13 different fishing grounds. Anchovy consistently appeared from April to December and reached the production of 840 to 1,424 t (average : 1,228 t), with a big annual variation. However, anchovy production in this area accounts for approximately 75% of the production by set net in Anggang Bay whereas it holds 6.8% of the nationwide production (=18,034 t) by set net. The school of anchovy starts to appear in April at the west mouth of the bay and move north-eastward. Afterward, having three of clockwise turns in the middle of the bay, they scatter to swim into the deep place of the bay and finally go out to turn back to their coming way. These behaviors of anchovy are likely related to thermal fronts as well as distributions of food. The production of anchovy (y, kg) relied greatly on frequencies of effective northeasterly ($x_1$) or northwesterly ($x_2$) wind (${\leq}$3.5m/s) which blow between April and June. Their relationships are as follows: $y=1086.27+21.499x_1-15.16x_2$ (r=0.901). Consequently, we concluded that the northeasterly wind, which appears in the breeding season of spring, played a role to retard the movement of anchovy school to the eastern sea, while the northwesterly wind inhibits the invasion of anchovy school into the bay.