• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.954-960
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• 2008

MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1991년도 하계학술대회 논문집
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• pp.102-105
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• 1991

In recognition of the transportation problems of the present and to prepare for the ever increasing demands of the future, government decided to develop the magnetically levitated train domestically and started R&D program office in Korea Institute of Machinery and Metals(KIMM). This office since has established three step by step goals : first to develop a 40 passenger exhibition vehicle for Daejon EXPO'93, second to develop the low to mid-speed maglev system for urban public transportation by 1997 and finally the high speed inter-city maglev train by year 2001. The first two maglev systems will use attractive levitation-LIM driven technologies and these technologies are the ones currently being developed by this office and others. The maglev train system is a product of wide range of technologies from electro-technologies to civil engineering technologies. Some of the technologies are currently available but more have to be developed in the near future and these technologies are owned by or to be developed by various institutions within the science & technology community. The level of the technologies available at the present time are still very rudimentary and their basis are very narrow. Recently we have made a few successes in terms of levitation and propulsion but they are only with small scale modules and results are very qualitative at best. A great deal of development work has yet to be done to refine the technologies and to gain confidence. Full scale levitation/propulsion modules will be tested on the curved guideway within 6 months by this office and another institution. This paper reviews the current status of the maglev technologies in Korea and discuss the development strategies. The Korean maglev program is very ambitious and the schedule is even more so. A steady financial support and strong system engineering and integration are essential to the success of this program.

• IMMUNE NETWORK
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• 제11권5호
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• pp.268-280
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• 2011

Background: Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-${\gamma}$ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.

• The Journal of Advanced Prosthodontics
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• 제4권3호
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• pp.153-157
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• 2012

PURPOSE. The study was conducted to compare the radiographic and clinical methods of measuring the horizontal condylar guidance (HCG) values. MATERIALS AND METHODS. The condylar guidance was measured using the radiographic (CT scan) and three clinical methods i.e. the wax protrusive records, Lucia jig record and intraoral central bearing device in 12 patients aged between 20-40 years irrespective of sex. The records were taken and transferred on the semi-adjustable articulator to record the HCG values. The CT scan was taken for 3D reconstruction of the mid facial region. Frankfort horizontal plane (FHP) and a line extending from the superior anterior most point on the glenoid fossa to the most convex point on the apex of articular eminence (AE) was marked on the CT scan. An angle between these two lines was measured on both right and left sides to obtain condylar inclination angle. Three interocclusal protrusive wax and jig records were taken and transferred to the semi adjustable articulator. Three readings were recorded on each side. Similarly the records were taken and transferred to the same articulator using the intra oral central bearing device to record the readings. RESULTS. The statistical analysis showed insignificant differences in the HCG values between the right and left sides [(P=.589 (CT), P=.928 (wax), P=.625 (jig), P=.886 (tracer)]. The clinical methods provided low Pearsons correlation values [(R = 0.423 (wax), R = 0.354 (jig), R = 0.265 (tracer)] for the right as well as the left sides when compared with the CT values. Among the clinical methods, jig and wax method showed strong level of association which is statistically significant while the intra-oral tracer showed weak association with the other two methods. CONCLUSION. The right and left HCG values were almost similar. The CT scan showed higher HCG values than the clinical methods and among the clinical methods, values obtained from all the methods were comparable.

• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.835-843
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• 2022

Deinococcus radiodurans is an extremophilic bacterium that can thrive in harsh environments. This property can be attributed to its unique metabolites that possess strong antioxidants and other pharmacological properties. To determine the potential of D. radiodurans R1 lysate (DeinoLys) as a pharmacological candidate for inflammatory bowel disease (IBD), we investigated the antiinflammatory activity of DeinoLys in bone marrow-derived dendritic cells (BMDCs) and a colitis mice model. Lipopolysaccharide (LPS)-stimulated BMDCs treated with DeinoLys exhibited alterations in their phenotypic and functional properties by changing into tolerogenic DCs, including strongly inhibited proinflammatory cytokines (TNF-α and IL-12p70) and surface molecule expression and activated DC-induced T cell proliferation/activation with high IL-10 production. These phenotypic and functional changes in BMDCs induced by DeinoLys in the presence of LPS were abrogated by IL-10 neutralization. Furthermore, oral administration of DeinoLys significantly reduced clinical symptoms against dextran sulfate sodium-induced colitis, including body weight loss, disease activity index, histological severity in colon tissue, and lower myeloperoxidase level in mice. Our results establish DeinoLys as a potential anti-inflammatory candidate for IBD therapy.

• Genomics & Informatics
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• 제14권3호
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• pp.112-124
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• 2016

Solid tumor is generally observed in tissues of epithelial or endothelial cells of lung, breast, prostate, pancreases, colorectal, stomach, and bladder, where several genes transcription is regulated by the microRNAs (miRNAs). Argonaute (AGO) protein is a family of protein which assists in miRNAs to bind with mRNAs of the target genes. Hence, study of the binding mechanism between AGO protein and miRNAs, and also with miRNAs-mRNAs duplex is crucial for understanding the RNA silencing mechanism. In the current work, 64 genes and 23 miRNAs have been selected from literatures, whose deregulation is well established in seven types of solid cancer like lung, breast, prostate, pancreases, colorectal, stomach, and bladder cancer. In silico study reveals, miRNAs namely, miR-106a, miR-21, and miR-29b-2 have a strong binding affinity towards PTEN, TGFBR2, and VEGFA genes, respectively, suggested as important factors in RNA silencing mechanism. Furthermore, interaction between AGO protein (PDB ID-3F73, chain A) with selected miRNAs and with miRNAs-mRNAs duplex were studied computationally to understand their binding at molecular level. The residual interaction and hydrogen bonding are inspected in Discovery Studio 3.5 suites. The current investigation throws light on understanding miRNAs based gene silencing mechanism in solid cancer.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2001년도 학술대회지
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• pp.462-467
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• 2001

최근 민간뿐만 아니라 공공부문에서도 경쟁력 제고를 위하여 지식정보화에 대한 관심이 고조되고 있다 건설산업은 타산업에 비하여 제품이나 프로세스 측면에서 지식의 활용도가 높은 분야이다. 건설산업의 경우 R&D 투자 측면에 있어 IMF 이전으로 회복하지 못하고 있으며, 기술수준은 선진국 대비 4.6년의 격차를 보이고 있다. 정보인프라 또한 타산업에 비해 투자와 정보이용률이 저조하며, 지식경영에 있어서는 비전 수립과 정보기술 활용에 있어서 높은 수준을 보이고 있으나 지식의 공유와 평가 활동은 부진한 것으로 파악되었다. 건설산업의 지식정보화 기반 육성을 위해서는 지속적인 투자, 지식 공유에 대한 마인드 고취를 위한 교육, 지식 측정 및 평가를 위한 제도 마련 그리고 조직 문화의 육성이 요구된다.

• Advances in Energy Research
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• 제4권3호
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• pp.189-202
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• 2016

This work emphasized optimum production of biodiesel using non-edible Prunus armeniaca (Bitter Apricot) oil via transesterification collected from the high altitude areas of Himachal Pradesh, India. In this study the author produced biodiesel through the process of transesterification by using an alkali catalyst with alcohol (methanol and ethanol), under the varying molar ratio (1:6, 1:9, 1:12), variable catalyst percentage (1% and 2%) and temperature ($70^{\circ}C$, $75^{\circ}C$, $80^{\circ}C$, $85^{\circ}C$). Furthermore, a few strong base catalysts were used that includes sodium hydroxide, potassium hydroxide, sodium metal and freshly prepared sodium methoxide. After screening the catalyst, response surface methodology (RSM) in connection with the central composite design (CCD) was used to statistically evaluate and optimize the biodiesel production operation using NaOH as catalyst. It was found that the production of biodiesel achieved an optimum level biodiesel yield with 97.30% FAME conversion under the following reaction conditions: 1) Methanol/oil molar ratio: 1:6, 2) Reaction time: 3h, 3) Catalyst amount: NaOH 2 wt. %, and 4) Reaction temperature: $85^{\circ}C$. The experimental results showed that the optimum production and conversion of biodiesel through the process of transesterification could be achieved under an optimal set of reaction conditions. The biodiesel obtained showed appropriate fuel properties as specified in ASTM, BIS and En- standards.

• 치위생과학회지
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• 제6권3호
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• pp.147-152
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• 2006

기존 치과위생사 인력의 이직을 줄이고 유효 치과위생사 인력을 많이 활용할 수 있는 방안을 마련함과 동시에 경영주체들의 경영개선을 위한 노력에 일조가 될 수 있는 기초 자료로서 활용하고자 문헌과 자료를 조사하고 각 지역의 구강진료기관에 종사하는 치과위생사를 대상으로 2004년 9월부터 12월까지 약 4개월 동안 이직의도와 직무만족간의 관계에 대한 설문조사를 실시하여 분석한 결과 다음과 같은 결론을 얻었다. 1. 직무만족도의 전체 평균은 $3.30{\pm}0.70$점으로 나타났으며, 각 영역별로 살펴보면 역할명료성요인이 $3.53{\pm}0.73$점으로 가장 높은 만족 수준을 보였고, 임금요인이 $3.14{\pm}0.70$점, 승진요인이 $3.18{\pm}0.80$점으로 낮은 만족 수준을 보였다. 2. 이직의도의 전체 평균은 $3.23{\pm}0.77$점으로 나타났으며, 일반적인 특성에 따른 이직의도 수준을 살펴본 결과, 연령별로는 21-25세가 가장 높은 이직의도 수준을 보였고(p < 0.001), 결혼여부별로는 미혼이 가장 높은 이직의도 수준을 보였으며(p < 0.001), 병원형태별로는 치과의원이 가장 높은 이직의도 수준을 보였다(p < 0.01). 근무경력별로는 1-3년이 가장 높은 이직의도 수준을 보였고(p < 0.001), 현 직장에서의 근무연수별로는 1-3년이 가장 높은 이직의도 수준을 보였으며(p < 0.001), 월 평균 급여별로는 130-160만원이 가장 높은 이직의도 수준을 보였다(p < 0.01). 3. 이직의도와 직무만족도와의 상관관계를 살펴본 결과, 이직의도와 직무만족도 요인은 모두 역 상관관계를 나타내고 있고, 특히 임금요인(r = -0.249, p < 0.01)과 상호관계요인(r = -0.218, p < 0.01)에서 비교적 높은 상관관계를 보였으며, 직무만족도에 관한 요인에서는 역할명료성 요인과 직무내용요인이 높은 상관관계를 보였고(r = 0.612, p < 0.01), 직무만족도 요인과는 직무내용요인이 높은 상관관계를 보였다(r = 0.759, p < 0.01).

• Journal of Nutrition and Health
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• 제31권9호
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• pp.1457-1467
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• 1998

The relationship between cognitive function and dietary patterns in Korean elderly women was investigated in this study. Twenty-four-hour dietary recall and cognitive function tests were performed in 170 elderly women aged over 60 years. Average age of the subjects was 71 years and the average number of family members was 3.3. Most of the subjects had less than seven years of education and their monthly income was much lower than the Korean average. Most of the subjects engaged in regular exercise and did not indulge in drinking and smoking. The average cognitive function score of the subjects was 58.4. Most of the subjects appeared to consume insufficient amount of food ; average intakes of energy, Ca, iron, vitamin A, and riboflavin were lower than RDA for the elderly. Cognitive function score was positively related to education level and regularity of exercise. Also, cognitive function score had a strong relationship with food intake such as total amounts of foods, fish and shellfish, milk and dairy products, total animal food, fruit, bread and sugar. Energy, protein, Ca, p, riboflavin, and niacin were also shown to have positive relationships to cognitive function score, while carbohydrate caloric ratio had a negative relationship with cognitive function. According to the results of multiple regression analysis, factors that most influenced on cognitive function were education level and riboflavin intake. The subjects who had more than seven years of education had significantly higher cognitive function scores than those with no school education. And cognitive function scores f3r those who consumed more than 2/3 of the RDA for most nutrients were significantly higher than the ones fir the subjects who consumed less than 2/3 of the RDA. These results suggest that proper education and adequate nutrient intake in quantity and quality by achieving food diversity are essential in maintaining cognitive function in later life (Korean J Nutrition 31(9) : 1457-1467, 1998)