• Korean Journal of Pharmacognosy
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• v.44 no.1
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• pp.16-21
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• 2013

The fruits of Pourthiaea villosa were extracted with methanol and its extract was fractionated with n-hexane, methylene chloride, ethyl acetate and n-butanol. Repeated column chromatography of silica gel, sephadex LH-20 and HPLC led to the isolation of nine phenolic compounds from ethyl acetate soluble fraction. The chemical structures were elucidated as kaemferol-3-O-${\beta}$-D-glucopyranoside (astragalin) (1), isorhamnetin-3-O-${\beta}$-D-glucopyranoside (2), kaempferol-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\alpha}$-L-rhamnopyranoside (3), caffeic acid (4), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-a-L-rhamnopyranoside (5), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-glucopyranoside (6), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-galactopyranoside (7), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (quercitrin) (8), and kaempferol-3-O-${\alpha}$-L-rhamnopyranoside (afzelin) (9) by spectroscopic techniques. These compounds were isolated from this plant for the first time.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.6
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• pp.901-905
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• 1995

Three flavonoid compounds, astragalin(1), isoquercitrin(2) and quercetin $3-O-{\beta}-D-xylopyranosyl(1-2)-{\beta}-D-glucopyranoside(3)$ isolated from the leaves of Eucommia ulmoides were identified and quantified by HPLC. All flavonoids were well separated on a ${\mu}-Bondapak\;C_{18}$ column with a mobile phase composed of THF-dioxane-MeOH-HOAc-5% $H_3PO_4-H_2O$(145 : 125 : 50 : 20 : 2 : 658). The contents of compound 1 in the methanol extract and n-butanol fraction were 0.09%(w/w) and 0.46%(w/w), of compound 2 were 0.08%(w/w) and 0.48%(w/w), and of compound 3 were 0.40%(w/w) and 1.22%(w/w), respectively.

• Korean Journal of Pharmacognosy
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• v.33 no.3 s.130
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• pp.194-199
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• 2002

The radix and folium of Eucommia ulmoides(Eucommiaceae) has been used for backache, atrophy of the leg and knee, enuresis, hypertension. In order to evaluate to quality of folium, we have been isolated a main compound as a standard compound, which was elucidated $quercetin-3-O-{\beta}-D-xylopyranosyl(l{\rightarrow}2)-{\beta}-D-glucopyranoside$ through it's physicochemical data and IR, FAB-Mass, $^{13}C-NMR$ and $^{1}H-NMR$ analysis. It was analyzed by HPLC system using 17% $CH_3CN$ as a solvent system. The amount of $quercetin-3-O-{\beta}-D-xylopyranosyl(l{\rightarrow}2)-{\beta}-D-glucopyranoside$ from Eucommiae folium was in the range of $0.056{\pm}0.022$ mg(n=7) and also from the results of analysis through seasonal variation$(June{\sim}October)$ September and October have been evaluated to be very high in it's content.

• Korean Journal of Pharmacognosy
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• v.34 no.4 s.135
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• pp.344-351
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• 2003

Phytochemical examination of seeds of Astragalus sinicus has led to the isolation and characterization of kaempferol $3-O-{\beta}-D-apiofuranosyl-(1{\rightarrow}2)-{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (1), quercetin $3-O-{\beta}-D-xylopyranosyl-(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (2), ampelopsin (3), ampelopsin $3'-O-{\beta}-D-xylopyranoside$ (4), ampelopsin $3'-O-{\beta}-Dxylopyranoside$ (5), myricetin (6), myricetin $3'-O-{\beta}-D-glucopyranoside$ (7), myricetin $3'-O-{\beta}-D-xylopyranoside$ (8). Antioxidative activity of these compounds was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Flavanonols(3,4, and 5) showed potent antioxidative activity.

• YAKHAK HOEJI
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• v.41 no.1
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• pp.1-6
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• 1997

Rubus coreantim (Rosaceae), which has been used in oriental traditional medicine as the remedies for impotence, pollution and premature ejaculation, bet-wetting and frequ ency of urination. is a fruit drug cultivating throughout southern Korea. The leaves of Rubus coreanum were extracted with 80% acetone. The extracts were separated by Ambellite XAD-2, Sephadex LH-20. MCI-gel CHP 20P and Cosmosil 75 C$_{18}$ OPN. And three flavonoids were isolated. The structures of these compounds were elucidated as quercetin-3-O-${\beta}$-D-glueuronopyranoside(l). quercetin-3-O-${\beta}$-D-glueuronopyranosyl methyl ester(2), quercetin-3-O-${\beta}$-D-xylopyranosyl-(2${\rightarrow}$1)-${\beta}$-glucopyranoside(3) on the basis of physicochemical and spectroscopic evidences.