• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.800-811
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• 2022

The integration of metabolomics and transcriptomics may elucidate the correlation between the genotypic and phenotypic patterns in organisms. In equine physiology, various metabolite levels vary during exercise, which may be correlated with a modified gene expression pattern of related genes. Integrated metabolomic and transcriptomic studies in horses have not been conducted to date. The objective of this study was to detect the effect of moderate exercise on the metabolomic and transcriptomic levels in horses. In this study, using nuclear magnetic resonance (NMR) spectroscopy, we analyzed the concentrations of metabolites in muscle and plasma; we also determined the gene expression patterns of branched chain (alpha) keto acid dehydrogenase kinase complex (BCKDK), which encodes the key regulatory enzymes in branched-chain amino acid (BCAA) catabolism, in two breeds of horses, Thoroughbred and Jeju, at different time intervals. The concentrations of metabolites in muscle and plasma were measured by ¹H NMR (nuclear magnetic resonance) spectroscopy, and the relative metabolite levels before and after exercise in the two samples were compared. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA), and variable important plots and t-test were used for basic statistical analysis. The stress-induced expression patterns of BCKDK genes in horse muscle-derived cells were examined using quantitative reverse transcription polymerase chain reaction (qPCR) to gain insight into the role of transcript in response to exercise stress. In this study, we found higher concentrations of aspartate, leucine, isoleucine, and lysine in the skeletal muscle of Jeju horses than in Thoroughbred horses. In plasma, compared with Jeju horses, Thoroughbred horses had higher levels of alanine and methionine before exercise; whereas post-exercise, lysine levels were increased. Gene expression analysis revealed a decreased expression level of BCKDK in the post-exercise period in Thoroughbred horses.

• International Journal of Computer Science & Network Security
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• v.22 no.8
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• pp.197-207
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• 2022

Given the prevalence of violence in Ukrainian families, measures to prevent parental abusive treatment against children are urgent. It is important to study today's youth awareness about violence within families in order to enhance a culture of engagement with spouses and children in future. The aim of the study is to examine students' reflective experiences and their attitudes towards forms, frequency, causes and effects of parental abusive treatment. During the research the following methods were used step-by-step: theoretical analyses of scientific resources, anonymous questionnaire, quantitative and correlation analyses. According to result of survey 98 students who were interviewed, none of them fell victim of sexual abuse in their families. However, more than a half (51%) of the students surveyed experienced some forms of parental physical punishment. All the interviewed students encountered psychological cases of parental violence. The mostprevalent forms of parental abuse among the interviewees are criticism, negative comparison, emotional detachment, arrogance, intimidation, blackmail and humiliation. The most prevalent consequences of parental abuse among students are anxiety, low self-esteem, insecurity, impatience, suspiciousness, constraint in communication. Students agree that budget limitations, forced labor, criticism, spanking, emotional detachment, ignoring type of communication, reproach, blackmail are acceptable methods of punishment to use when raising their own children. These results clearly demonstrate the problem of the impact of parental abuse on children and its consequences in the future. A virtual dimension of the actualized problem is considered, namely: - virtualization of aggression and cruelty in the postmodern world. - the level of determining the factor of cruelty from the space of virtual culture. - the mirror image of everyday cruelty in the virtual environment; - the phenomenon of video games as a source and context of representation of the factor of cruelty in behavioral realities; - cybercrime as a virtualized result of cruelty in family and everyday realities. - futurological perspectives of virtualization of cruelty in communicative culture in general and in family relations in particular. The postmodern world is fundamentally different from the traditions and culture of the past, primarily due to the development of computer technologies and the virtualization of life in general. So, for example, virtual communities have become, in a certain way, another world, a second reality of life in general. And certain behavioral factors, in particular cruelty in the private environment, became a projection of such a phenomenon as cybercrime. Video games are a unique modern phenomenon, which multipolarly absorb all facets of human potential, communicative tendencies, behavioral and characterological factors, from the warmth of interpersonal relationships to the extreme degree of cruelty.

• Journal of Environmental Health Sciences
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• v.48 no.3
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• pp.159-166
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• 2022

Background: A quantitative structure-activity relationship (QSAR) model was adopted in the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH, EU) regulations as well as the Act on Registration, Evaluation, etc. of Chemicals (AREC, Republic of Korea). It has been previously used in the registration of chemicals. Objectives: In this study, we investigated the correlation between the predicted data provided by three prediction programs using a QSAR model and actual experimental results (acute fish, daphnia magna toxicity). Through this approach, we aimed to effectively conjecture on the performance and determine the most applicable programs when designating toxic substances through the AREC. Methods: Chemicals that had been registered and evaluated in the Toxic Chemicals Control Act (TCCA, Republic of Korea) were selected for this study. Two prediction programs developed and operated by the U.S. EPA - the Ecological Structure-Activity Relationship (ECOSAR) and Toxicity Estimation Software Tool (T.E.S.T.) models - were utilized along with the TOPKAT (Toxicity Prediction by Komputer Assisted Technology) commercial program. The applicability of these three programs was evaluated according to three parameters: accuracy, sensitivity, and specificity. Results: The prediction analysis on fish and daphnia magna in the three programs showed that the TOPKAT program had better sensitivity than the others. Conclusions: Although the predictive performance of the TOPKAT program when using a single predictive program was found to perform well in toxic substance designation, using a single program involves many restrictions. It is necessary to validate the reliability of predictions by utilizing multiple methods when applying the prediction program to the regulation of chemicals.

• Journal of the Society of Disaster Information
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• v.18 no.4
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• pp.861-872
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• 2022

The purpose of this study was to establish a complex disaster scenario that can comprehensively consider various disaster situations that may occur in the utility tunnel. Method: In order to comprehensively consider the correlation between disasters, a composite disaster scenario was derived from a combination of damage factors, respectively. A risk assessment was performed in order to derive the priorities of the scenarios. And based on the results, the priorities of complex disaster scenarios were set. Result: Based on the disaster cases in the utility tunnel, a plan was prepared for complex disaster scenarios centered on damage. A complex disaster scenario was specified using a semi-quantitative evaluation method for single and multiple disaster factors such as fire, flooding, and earthquake. Conclusion: The composite disaster scenario derived from this study can be used for the prevention and preparation of damage when the precursor symptoms of a disaster are detected. In addition, the results of this study are expected to be used as basic data for preparing strategic plans and preparing complex disaster response technologies to induce rapid response and recovery in case of emergency disasters.

• Journal of Industrial Convergence
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• v.20 no.12
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• pp.203-211
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• 2022

This study is a descriptive research study to investigate the mediating effect of self-leadership in the relationship between metacognition and proactivity of problem solving and the effect of metacognition on proactivity of problem solving of nursing students. 194 fourth-year nursing students who experienced in-school simulation practice at a university were selected as subjects for the study. Data were collected from September to December 2021 using a structured questionnaire. The collected data were analyzed by descriptive statistics, correlations and parametric regression using the SPSS 22.0 program. It was found that metacognition and problem-solving proactiveness and self-leadership had a quantitative correlation, and it was analyzed that metacognition and self-leadership had a significant effect on problem-solving proactiveness. Also, it was found that self-leadership is partially mediated in the relationship between metacognition and problem-solving proactiveness. According to the results of this study, it is necessary to develop a program that can improve the elements of metacognition and self-leadership in order to increase the problem-solving activity of nursing students through problem-centered learning integrated simulation education.

• Geophysics and Geophysical Exploration
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• v.26 no.2
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• pp.37-51
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• 2023

The ground roll is the most common coherent noise in land seismic data and has an amplitude much larger than the reflection event we usually want to obtain. Therefore, ground roll suppression is a crucial step in seismic data processing. Several techniques, such as f-k filtering and curvelet transform, have been developed to suppress the ground roll. However, the existing methods still require improvements in suppression performance and efficiency. Various studies on the suppression of ground roll in seismic data have recently been conducted using deep learning methods developed for image processing. In this paper, we introduce three models (DnCNN (De-noiseCNN), pix2pix, and CycleGAN), based on convolutional neural network (CNN) or conditional generative adversarial network (cGAN), for ground roll suppression and explain them in detail through numerical examples. Common shot gathers from the same field were divided into training and test datasets to compare the algorithms. We trained the models using the training data and evaluated their performances using the test data. When training these models with field data, ground roll removed data are required; therefore, the ground roll is suppressed by f-k filtering and used as the ground-truth data. To evaluate the performance of the deep learning models and compare the training results, we utilized quantitative indicators such as the correlation coefficient and structural similarity index measure (SSIM) based on the similarity to the ground-truth data. The DnCNN model exhibited the best performance, and we confirmed that other models could also be applied to suppress the ground roll.

• Analytical Science and Technology
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• v.21 no.1
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• pp.34-40
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• 2008

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of lercanidipine in human plasma. After addition of internal standard (amlodipine), plasma was precipitated with acetonitrile and the supernatant was evaporated. The residues were dissolved in 50 % acetonitrile and analyzed by LC-MS/MS. Using MS/MS with multiple reaction monitoring(MRM) mode, lercanindipine were selectively detected without severe interference from human plasma. The standard calibration curve for lercanidipine was linear (r = 0.9994) over the concentration range 0.05-20.0 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of lercanidipine was lower than 11.7 % (correlation of variance, CV), and accuracy was between 94.4-114.8 %. This method has been successfully applied to the pharmacokinetic study of lercanidipine in human plasma.

• Analytical Science and Technology
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• v.21 no.4
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• pp.284-295
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• 2008

For plastics samples, a method using combustion ion chromatography was selected as a method for rapid low-cost analysis to test whether hazardous substances are contained or not. Using combustion ion chromatography, a verification test for F, Cl and Br compounds generated a linear calibration curve with a correlation coefficient of $r^2$ = 0.999~1.000 in the calibration range from 0.5 to 4.0 mg/kg. The detection limits were found to be 0.005~0.024 mg/kg and quantitative limits were found to be 0.014~0.073 mg/kg. The recoveries of combustion ion chromatography using certified reference material (CRM) were found to be 95.5~104.9%. Based on these results, a proficiency test was conducted together with several laboratories in and out of the country, to make comparative analysis of the results from each laboratory. As a result, the data supported the use of combustion ion chromatography as an effective analysis method to deal with regulations for halogen-free electronic products and for other hazardous substances in the electronic products.

• 한국노년학
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• v.32 no.2
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• pp.415-429
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• 2012

This study attempts to develop the scale of 'Caregiving Affirmation Scale' for Korean Caregivers for the Aged and to testify the possibility of generalizing the scare. Mixed methods of qualitative and quantitative researches were used to achieve the purpose of the study. The steps of developing the scale were proceeded as follows: Firstly, 40 items were identified through the qualitative study and the literature reviews. Secondly, the content validity was verified through the professional group survey in addition to confirm the questionnaire items by the 1st survey. And thirdly, the construct validity and concurrent validity of the factors of 'Caregiving Satisfaction' were explored by performing the 2nd survey. As the results of the study, the total 16 items of 'Caregiving Affirmation scale' identified with the four sub-factors as follows; 1) Improvement of the care skill and a sense of fulfillment; 2) Possibility of contributions to other persons; 3) Acceptance of the aged; and 4) Improvement of Self-value. The reliability value of Cronbach's α was 0.89 with the high internal consistency and the confirmatory factor analysis showed the relationship between the items and each factor were also statistically significant. Thus, the content validity was verified. Also the correlation with the affirmative care recognition and the life satisfaction was 0.65, which meant the concurrent validity exists. The Caregiving Affirmation Scale for Korean Family Caregivers for the aged is expected to be used at the social welfare practice settings for the aged.

• Health Policy and Management
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• v.33 no.3
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• pp.311-324
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• 2023

Background: Long-term care insurance for the elderly has been stably established along with the quantitative expansion of long-term care facilities. Indeed, the need for a paradigm about human rights-based service approach is being raised throughout society from a service perspective. Therefore, this study aimed to analyze the association between elderly human rights awareness and quality of service by considering human rights education as a moderate variable. Methods: This study conducted surveys with 138 caregivers working in long-term care facilities located in Seoul and Gangwon. General characteristics, awareness of human rights, and the level of service quality were examined using descriptive statistics, frequency analysis, and correlation analysis. And multi-variable linear regression with a hierarchical framework was employed. These analyses were performed using IBM SPSS ver. 25.0. Results: Of the 138 caregivers, 97.1% were female, 87.7% were more than 50 years old, and most of their education level was high-school graduates. Their length of employment ranged from more than 5 years to less than 10 years. The level of awareness regarding elderly human rights of the elderly was below normal (mean=2.21), but the quality of service was high (mean=4.21), and the need for human rights education was also high (mean=4.28). Among the general characteristics, the length of employment was significantly associated with awareness of elderly human rights. Moreover, political rights awareness, included as sub-domains of human rights, was positively associated with quality of service. However, the moderating variable, human rights education, was not significantly associated with the quality of service. Conclusion: In this study, human rights education, as a moderating variable, did not have a statistically significant effect on caregivers' human rights awareness in relation to service quality. This finding is inconsistent with previous research results. These results can be explained by the fact that the frequency of education in long-term care facilities was a significant factor in the practice of protecting the human rights of the elderly. Therefore ongoing encouragement for the frequency of current human rights education and improvements in the educational approach appear to be necessary. In addition, these findings reveal the need for strength of education policies and effective in-depth research about human rights and quality of service to respect the human rights of the elderly.