• Journal of the Korean Society for information Management
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• v.27 no.4
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• pp.309-328
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• 2010

This study has analyzed the trend of the quantitative research by analyzing domestic dissertations on information literacy that were published since 2000. The procedures, regulations, and descriptive elements of the quantitative study were compared and analyzed for this study. In addition, the study used 5 variables and criterions to measure these items. Based on the calculations, the study has examined the general characteristics of the thesis, research method, sampling method and sampling population of the dissertations. The study has also analyzed the trend of the measurement variables by categorizing the characteristics by published year and majors. Furthermore, the study has also presented the trend of the usage of statistic analysis method on research purpose by classifying the method into each purpose.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Journal of the Optical Society of Korea
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• v.7 no.4
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• pp.234-239
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• 2003

Objective methods to assess quantitatively port wine stain (PWS) blanching in response to laser therapy are needed to improve laser therapeutic outcome. Previous studies have attempted to assess objectively PWS color based on point measurement devices. To date, these approaches have typically been limited by a number of factors such as small test area and need for contact. To address these issues, a polarization spectral imaging system and an image analysis method have been developed to evaluate quantitatively erythema and melanin content distribution in skin. The developed polarization spectral imaging system minimizes artifacts such as glaring, shadowing, and non-uniform illumination that interfere with image fidelity. Furthermore, the image analysis method has been employed to get images of skin melanin and erythema indices from the acquired color images for quantitative analysis. Finally, using PWS patient color image, the effectiveness in laser treatment of PWS was evaluated by calculating relative erythema index image that is the relative erythema index of PWS region to the normal region. The developed device and analysis method appears to be a simple and effective method for quantitative analysis of PWS blanching.

• Korean Journal of Pharmacognosy
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• v.30 no.2
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• pp.163-167
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• 1999

A method for isolation and qantitative determination of anemarsaponin B from the rhizomes of Anemarrhena asphodeloides has been developed. Isolation of anemarsaponin B was achieved by silica gel and RP-18 column chromatography. The HPLC method for quantitative determination of anemarsaponin B provided a method for standardization of the crude drug. It suggested that the content of anemarsaponin B in Anemarrhena asphodeloides is about 0.12-1.48%.

• Journal of the Korean Ceramic Society
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• v.23 no.2
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• pp.64-70
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• 1986

In this investigation x-ray diffraction method was mainly studied for quantitative analysis of clinker mineral composition. And also optical microscopic observation and Bogue calculation method were applied to compare with the x-ray diffraction method. In the procedure of x-ray diffraction analysis graphite monochromator automatic divergence slit and spinner for sample holder were used for minimizing the error due to the operation of the equipment. Especially the separation of overlapped peaks were proceeded by micro-processor automatically. The results of x-ray diffraction method for synthesized clinker were consistent with the Bogue value and the results of optical microscopic observation. However the results of quantitative analysis of mineral composition or commercial clinker containing solid solution of minor component were different from the Bogue value. On the other hand they agreed reasonably well with results of the optical mic-roscopic observation.

• Journal of the Korean Society of Safety
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• v.12 no.1
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• pp.113-121
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• 1997

Human reliability attempts to make precise quantitative analyses and predictions of the performance of human-machine(or product) systems. In order to yield more precise human error analysis, precise human error probabilities(HEPs) must be used in the analysis. However, because human behavior is influenced by factors that are called performance shaping factors(PSFs), the effects of PSFs must be considered to obtain precise HEPs, These are called basic HEPs or situation-specific HEPs. This paper presents a theoretical method for obtaining basic HEPs (i.e. , considering PSFs) in quantitative human error analysis. In this method, the weight which characterizes the degree of importance of several PSFs is obtained by the analytic hierarchy process. The quality scores of PSFs in the task situation are obtained by percentile concept. These scores are used in conjunction with the relative Importance weights of PSFs to compute the composite quality percentile score of PSFs in the task situation. Then, a new mapping method of the composite quality percentile score of PSFs into a situation-specific basic HEP is proposed with a numerical example.

• Journal of Information Technology Services
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• v.8 no.1
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• pp.113-124
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• 2009

Value-based requirements engineering process, called The ViRE(Value-Innovative Requirement Engineering) was suggested to create an uncontested market using ERRC(Eliminate, Reduce, Raise, Create) requirements analysis. But ViRE did not provides a quantitative data analysis method for ERRC decision so as to make objective decisions for customers. In this paper, to solve this problem, we suggest a quantitative ERRC analysis method by estimating requirements cost. Our method defines user requirements and decides their weight. Then, it makes quality level table for all the identified requirements and function modules and estimate implementation cost based on their quality levels. Finally, assess each requirement's impact and then evaluate ERRC value. We could get the more objective ERRC values by evaluate the requirement weight. functional module weight, and implementation cost. And we proved the efficiency of our model by a case study, smart student ID system.

• 한국정보디스플레이학회:학술대회논문집
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• 2002.08a
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• pp.115-117
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• 2002

In order to realize a stable rubbing process for the liquid crystal panel, the authors investigated the quantitative evaluation method of rubbing process uniformity. The proposed method focuses on the relationship between the image quality of the LCD panel for gray scale images and the rubbing uniformity. The proposed method indicates rubbing uniformity using quantitative parameters of spots and hairlines on the LCD panel.

• Journal of Microbiology
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• v.40 no.4
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• pp.274-281
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• 2002

The competitive quantitative PCR method targeting pcbC gene was developed for monitoring 4-chlorobiphenyl(4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The method involves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the elec-trophoresed PCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strain DJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with 4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCR in comparison with 4CB degradation and the result was compared to those obtained by using the conventional cultivation method. We successfully detected and monitored 4CB-degrading bacteria in each microcosm and found a significant linear relationship between the number of 4CB-degrading bacteria and the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experiments suggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CB- degrading bacteria appears to be rapid, sensitive and more suitable than the microbiological approach in estimating the capacity of 4CB biodegradation in environmental samples.

• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.367-376
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• 2011

BACKGROUND: In order to develop effective assessment method for Korean paddy soil microbial community structure, reliable genomic DNA extraction method from paddy soil and quantitative real-time PCR (qRT-PCR) method are needed to establish METHODS AND RESULTS: Out of six conventional soil genomic DNA extraction methods, anion exchange resin purification method was turn to be the most reliable. Various PCR primers for distinguishing five bacterial phylum (${\alpha}$-Proteobacteria, ${\beta}$-Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes), all bacteria, and all fungi were tested. Various qRT-PCR temperature conditions were also tested by repeating experiment. Finally, both genomic DNA extraction and qRT-PCR methods for paddy soil were well established. CONCLUSION: Quantitative real-time PCR (qRT-PCR) method to assess paddy soil microbial community was established.