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http://dx.doi.org/10.5338/KJEA.2011.30.4.367

Assessment of Korean Paddy Soil Microbial Community Structure by Use of Quantitative Real-time PCR Assays  

Choe, Myeong-Eun (School of Applied Biosciences, Kyungpook National University)
Lee, In-Jung (School of Applied Biosciences, Kyungpook National University)
Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University)
Publication Information
Korean Journal of Environmental Agriculture / v.30, no.4, 2011 , pp. 367-376 More about this Journal
Abstract
BACKGROUND: In order to develop effective assessment method for Korean paddy soil microbial community structure, reliable genomic DNA extraction method from paddy soil and quantitative real-time PCR (qRT-PCR) method are needed to establish METHODS AND RESULTS: Out of six conventional soil genomic DNA extraction methods, anion exchange resin purification method was turn to be the most reliable. Various PCR primers for distinguishing five bacterial phylum (${\alpha}$-Proteobacteria, ${\beta}$-Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes), all bacteria, and all fungi were tested. Various qRT-PCR temperature conditions were also tested by repeating experiment. Finally, both genomic DNA extraction and qRT-PCR methods for paddy soil were well established. CONCLUSION: Quantitative real-time PCR (qRT-PCR) method to assess paddy soil microbial community was established.
Keywords
Paddy soil microbial community; Quantitative real-time PCR;
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