• 정보관리학회지
• /
• 제27권4호
• /
• pp.309-328
• /
• 2010

이 연구는 2000년 이후 발표된 정보활용능력 분야의 국내 학위논문을 분석함으로써, 양적연구의 동향과 흐름을 분석하였다. 이를 위해 양적연구 동향을 분석하였다. 양적 연구를 위해 양적 연구 과정과 양적조사 관련 규정, 양적연구 관련 기술요소를 비교분석하였다. 또한, 각 요소 측정을 위해 5개 변인과 기준을 사용하였다. 이를 바탕으로 논문에 대한 연구주제, 조사방법, 표집방법, 표본대상, 표본크기에 대한 일반적 특징을 살펴보고, 이를 연도별, 전공별로 구분하여 측정변인에 대한 동향을 분석하였다. 또한 연구에서 사용한 통계분석방법을 목적에 따라 분류하여 연구목적에 따른 통계분석기법의 사용동향을 제시하였다.

• 농업과학연구
• /
• 제49권4호
• /
• pp.795-807
• /
• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Journal of the Optical Society of Korea
• /
• 제7권4호
• /
• pp.234-239
• /
• 2003

Objective methods to assess quantitatively port wine stain (PWS) blanching in response to laser therapy are needed to improve laser therapeutic outcome. Previous studies have attempted to assess objectively PWS color based on point measurement devices. To date, these approaches have typically been limited by a number of factors such as small test area and need for contact. To address these issues, a polarization spectral imaging system and an image analysis method have been developed to evaluate quantitatively erythema and melanin content distribution in skin. The developed polarization spectral imaging system minimizes artifacts such as glaring, shadowing, and non-uniform illumination that interfere with image fidelity. Furthermore, the image analysis method has been employed to get images of skin melanin and erythema indices from the acquired color images for quantitative analysis. Finally, using PWS patient color image, the effectiveness in laser treatment of PWS was evaluated by calculating relative erythema index image that is the relative erythema index of PWS region to the normal region. The developed device and analysis method appears to be a simple and effective method for quantitative analysis of PWS blanching.

• 생약학회지
• /
• 제30권2호
• /
• pp.163-167
• /
• 1999

A method for isolation and qantitative determination of anemarsaponin B from the rhizomes of Anemarrhena asphodeloides has been developed. Isolation of anemarsaponin B was achieved by silica gel and RP-18 column chromatography. The HPLC method for quantitative determination of anemarsaponin B provided a method for standardization of the crude drug. It suggested that the content of anemarsaponin B in Anemarrhena asphodeloides is about 0.12-1.48%.

• 한국세라믹학회지
• /
• 제23권2호
• /
• pp.64-70
• /
• 1986

In this investigation x-ray diffraction method was mainly studied for quantitative analysis of clinker mineral composition. And also optical microscopic observation and Bogue calculation method were applied to compare with the x-ray diffraction method. In the procedure of x-ray diffraction analysis graphite monochromator automatic divergence slit and spinner for sample holder were used for minimizing the error due to the operation of the equipment. Especially the separation of overlapped peaks were proceeded by micro-processor automatically. The results of x-ray diffraction method for synthesized clinker were consistent with the Bogue value and the results of optical microscopic observation. However the results of quantitative analysis of mineral composition or commercial clinker containing solid solution of minor component were different from the Bogue value. On the other hand they agreed reasonably well with results of the optical mic-roscopic observation.

• 한국안전학회지
• /
• 제12권1호
• /
• pp.113-121
• /
• 1997

Human reliability attempts to make precise quantitative analyses and predictions of the performance of human-machine(or product) systems. In order to yield more precise human error analysis, precise human error probabilities(HEPs) must be used in the analysis. However, because human behavior is influenced by factors that are called performance shaping factors(PSFs), the effects of PSFs must be considered to obtain precise HEPs, These are called basic HEPs or situation-specific HEPs. This paper presents a theoretical method for obtaining basic HEPs (i.e. , considering PSFs) in quantitative human error analysis. In this method, the weight which characterizes the degree of importance of several PSFs is obtained by the analytic hierarchy process. The quality scores of PSFs in the task situation are obtained by percentile concept. These scores are used in conjunction with the relative Importance weights of PSFs to compute the composite quality percentile score of PSFs in the task situation. Then, a new mapping method of the composite quality percentile score of PSFs into a situation-specific basic HEP is proposed with a numerical example.

• 한국IT서비스학회지
• /
• 제8권1호
• /
• pp.113-124
• /
• 2009

Value-based requirements engineering process, called The ViRE(Value-Innovative Requirement Engineering) was suggested to create an uncontested market using ERRC(Eliminate, Reduce, Raise, Create) requirements analysis. But ViRE did not provides a quantitative data analysis method for ERRC decision so as to make objective decisions for customers. In this paper, to solve this problem, we suggest a quantitative ERRC analysis method by estimating requirements cost. Our method defines user requirements and decides their weight. Then, it makes quality level table for all the identified requirements and function modules and estimate implementation cost based on their quality levels. Finally, assess each requirement's impact and then evaluate ERRC value. We could get the more objective ERRC values by evaluate the requirement weight. functional module weight, and implementation cost. And we proved the efficiency of our model by a case study, smart student ID system.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2002년도 International Meeting on Information Display
• /
• pp.115-117
• /
• 2002

In order to realize a stable rubbing process for the liquid crystal panel, the authors investigated the quantitative evaluation method of rubbing process uniformity. The proposed method focuses on the relationship between the image quality of the LCD panel for gray scale images and the rubbing uniformity. The proposed method indicates rubbing uniformity using quantitative parameters of spots and hairlines on the LCD panel.

• Journal of Microbiology
• /
• 제40권4호
• /
• pp.274-281
• /
• 2002

The competitive quantitative PCR method targeting pcbC gene was developed for monitoring 4-chlorobiphenyl(4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The method involves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the elec-trophoresed PCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strain DJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with 4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCR in comparison with 4CB degradation and the result was compared to those obtained by using the conventional cultivation method. We successfully detected and monitored 4CB-degrading bacteria in each microcosm and found a significant linear relationship between the number of 4CB-degrading bacteria and the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experiments suggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CB- degrading bacteria appears to be rapid, sensitive and more suitable than the microbiological approach in estimating the capacity of 4CB biodegradation in environmental samples.

• 한국환경농학회지
• /
• 제30권4호
• /
• pp.367-376
• /
• 2011

논 토양의 미생물 생태 다양성을 조사하기 위한 효과적인 방법으로 qRT-PCR을 적용하고자 본 연구를 수행하였다. 논 토양 미생물의 gDNA를 분리하기 위하여 Mo Bio kit를 사용한 효과적이고 안정적인 gDNA 분리 방법을 확립하였다. 논 토양 미생물 다양성을 qRT-PCR로 검출하기 위하여 bacteria를 세분한 ${\alpha}$-Proteobacteria, ${\beta}$-Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes 다섯 가지 문과 전체 bacteria, 전체 fungi를 구분할 수 있는 특이 primer set을 선정하여 다양한 조건의 시험을 통하여 최종 조건을 확립하였으며 재현성 실험을 통하여 방법의 유의성을 검증하였다.