• 한국콘텐츠학회논문지
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• 제21권9호
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• pp.125-135
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• 2021

중국은 캐릭터산업의 중요성과 가치에 대한 국가적인 공감대에 형성이 약하고 국가 전략산업의 가치 인식의 미미로 인하여 캐릭터 산업의 발전에 큰 어려움을 겪고 있다. 본 연구의 목적은 중국 캐릭터 산업의 경제적 효과를 분석하여 향후 캐릭터 산업의 정책수립에 일조하는데 있다. 이를 위해 본 연구는 중국 2017년 산업연관표를 활용하였다. 분석결과는 다음가 같다. 중국 캐릭터산업의 생산유발효과는 열 합계 3.45514,행 합계 1.30015로 타 산업보다 낮은 생산유발효과를 보이고 있는 것은 중국 캐릭터 산업이 아직 자기자본비율이 낮은 중소기업에서 생산되고 있음을 알 수 있다. 둘째, 캐릭터 산업은 전방연쇄효과를 나타내는 감응도 계수가 0.01426, 영향력계수는 0.03790로 모두 1보다 작은 최종수요적 제조업형이라고 할 수 있다. 셋째, 중국 캐릭터 산업의 소득유발는 0.47690, 생산세 유발효과는 -0.04912로 이는 캐릭터 산업이 타 산업보다 전체 산업에서 최종수요가 한 단위 증가될 때마다 발생하는 소득유발과 세부담이 적음을 나타낸다. 중국의 캐릭터 산업은 양적이 성장에도 불구하고 타 산업에 미치는 영향은 낮고 소득창출산업으로서의 역할을 하지 못하고 있다. 중국의 캐릭터 산업의 질적 발전을 위해서는 구조적 개선이 필요하다.

• Animal Bioscience
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• 제34권6호
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• pp.1078-1087
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• 2021

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

• Korean Journal of Radiology
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• 제19권6호
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• pp.1119-1129
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• 2018

Objective: To compare the image quality of low-tube-voltage and low-iodine-concentration-contrast-medium (LVLC) computed tomography urography (CTU) with iterative reconstruction (IR) with that of conventional CTU. Materials and Methods: This prospective, multi-institutional, randomized controlled trial was performed at 16 hospitals using CT scanners from various vendors. Patients were randomly assigned to the following groups: 1) the LVLC-CTU (80 kVp and 240 mgI/mL) with IR group and 2) the conventional CTU (120 kVp and 350 mgI/mL) with filtered-back projection group. The overall diagnostic acceptability, sharpness, and noise were assessed. Additionally, the mean attenuation, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and figure of merit (FOM) in the urinary tract were evaluated. Results: The study included 299 patients (LVLC-CTU group: 150 patients; conventional CTU group: 149 patients). The LVLC-CTU group had a significantly lower effective radiation dose ($5.73{\pm}4.04$ vs. $8.43{\pm}4.38mSv$) compared to the conventional CTU group. LVLC-CTU showed at least standard diagnostic acceptability (score ${\geq}3$), but it was non-inferior when compared to conventional CTU. The mean attenuation value, mean SNR, CNR, and FOM in all pre-defined segments of the urinary tract were significantly higher in the LVLC-CTU group than in the conventional CTU group. Conclusion: The diagnostic acceptability and quantitative image quality of LVLC-CTU with IR are not inferior to those of conventional CTU. Additionally, LVLC-CTU with IR is beneficial because both radiation exposure and total iodine load are reduced.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.257-264
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• 2019

Objective: Dairy cattle nutrient requirement systems acknowledge amino acid (AAs) requirements in aggregate as metabolizable protein (MP) and assume fixed efficiencies of MP used for milk protein. Regulation of mammary protein synthesis may be associated with AA input and milk protein output. The aim of this study was to evaluate the effect of nanoemulsified methionine and cysteine on the in-vitro expression of milk protein (casein) in bovine mammary epithelial cells (MAC-T cells). Methods: Methionine and cysteine were nonionized using Lipoid S 75 by high-speed homogenizer. The nanoemulsified AA particle size and polydispersity index were determined by dynamic light scattering correlation spectroscopy using a high-performance particle sizer instrument. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to determine the cytotoxicity effect of AAs with and without nanoionization at various concentrations (100 to $500{\mu}g/mL$) in mammary epithelial cells. MAC-T cells were subjected to 100% of free AA and nanoemulsified AA concentration in Dulbecco's modified Eagle medium/nutrient mixture F-12 (DMEM/F12) for the analysis of milk protein (casein) expression by the quantitative reverse transcription polymerase chain reaction method. Results: The AA-treated cells showed that cell viability tended to decrease (80%) in proportion to the concentration before nanogenesis, but cell viability increased as much as 90% after nanogenesis. The analysis of the expression of genetic markers related to milk protein indicated that; ${\alpha}_{s2}$-casein increased 2-fold, ${\kappa}$-casein increased 5-fold, and the amount of unchanged ${\beta}$-casein expression was nearly doubled in the nanoemulsified methionine-treated group when compared with the free-nanoemulsified methionine-supplemented group. On the contrary, the non-emulsified cysteine-administered group showed higher expression of genetic markers related to milk protein ${\alpha}_{s2}$-casein, ${\kappa}$-casein, and ${\beta}$-casein, but all the genetic markers related to milk protein decreased significantly after nanoemulsification. Conclusion: Detailed knowledge of factors, such nanogenesis of methionine, associated with increasing cysteine and decreasing production of genetic markers related to milk protein (casein) will help guide future recommendations to producers for maximizing milk yield with a high level of milk protein casein.

• 대한치과의사협회지
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• 제57권10호
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• pp.560-568
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• 2019

Purpose: This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans(S.mutans), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in $30m{\ell}$ of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. $1.8m{\ell}$ of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and $0.05mg/m{\ell}$, was mixed with $0.2m{\ell}$ of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 nm LED was used to expose light for 5 minutes to irradiate 59 nW energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with $0.05mg/m{\ell}$ curcuma powder extract, the concentration of $0.5mg/m{\ell}$ and LED light of 405 nm wavelength (p=0.000, p=0.003). $0.05mg/m{\ell}$ of curcuma powder extract and the concentration of $0.5mg/m{\ell}$ showed 100% antibacterial effect when exposed to light, whereas the concentration of $5mg/m{\ell}$ showed 11.95% antibacterial effect. When exposed to light, $0.05mg/m{\ell}$ of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of $0.5mg/m{\ell}$ and $5mg/m{\ell}$ did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans, no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.

• 터널과지하공간
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• 제29권4호
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• pp.262-279
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• 2019

고준위방사성폐기물처분장의 공학적 방벽에서는 다양한 원인으로 인해 기체가 발생한다. 만약 기체 생성 속도가 기체 확산 속도보다 빠를 경우 기체의 압력이 증가하게 되고, 기체 유입 압력(gas entry pressure)을 넘어서게 되면 기체가 급격히 벤토나이트 완충재를 통과하는 기체 이동 현상(gas migration)이 발생하게 되며 이는 사람과 주변 환경을 방사능에 노출시킬 수 있기 때문에, 공학적 방벽의 장기 건전성 확보 측면에서 기체 이동 현상을 명확히 규명하는 것이 매우 중요하다. 특히 벤토나이트 완충재와 같이 점토 물질을 다량 함유한 매질에서만 나타나는 매우 중요한 기체 흐름 현상인 팽창 흐름에 대한 수리-역학적 메커니즘을 규명하고, 기체 이동 현상의 정량적 평가를 위한 새로운 수치 해석 기법 개발 및 검증이 필수적이다. 따라서 본 연구에서는 공학적 방벽에서의 기체 이동 현상을 모사하고자 역학 손상 모델 및 손상도를 고려한 2상 유동 모델을 개발하였으며, 일정 체적 경계 조건 하에서의 1차원 기체 주입 시험 모사를 통해 개발된 모델의 적용성을 검토하였다. 수치 해석 결과 공극 수압 및 응력, 기체 유출량이 팽창 흐름 발생 시 급격히 증가하는 현상을 모사할 수 있었다.

• 한국수산과학회지
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• 제52권1호
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• pp.13-18
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• 2019

We determined the contents of 11 biogenic amines in 20 types of cabbage kimchi, 10 types of fish sauce, and 40 types of salted fish purchased from small- and medium-sized grocery stores in Gyeonggi-Do, Republic of Korea, from June to October 2017. The content of each of the biogenic amines in the various types of cabbage kimchi was slightly lower than that in detection amounts of each of the 10 types of biogenic amines and the values reported by Joe et al. The histamine content of all five types of anchovy sauce was lower than the limit set by the European Union (400 mg/kg), but that of three of the five types of sand lance sauce was higher. Analyzing the ratios of the contents of five highly toxic biogenic amines to those of the 11 biogenic amines yielded no quantitative correlation. The estimated daily intake of the five highly toxic biogenic amines in cabbage-kimchi and fermented fish was ${\leq}6mg$.

• 벤처창업연구
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• 제14권2호
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• pp.31-46
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• 2019

신성장 동력원으로서 벤처기업의 중요성이 증대됨에 따라 정부에서는 건강한 벤처 및 창업 생태계 구축을 위하여 다양하게 노력하고 있으며, 경쟁력 있는 벤처기업을 육성하고자 금융 및 연구개발 비용지원 등 다양한 지원정책을 확대하여 추진하고 있다. 따라서 본 연구에서는 자원기반이론을 바탕으로 벤처기업의 성장단계에 따라 정부지원자금 수혜경험이 내부역량과 경영성과에 어떠한 영향을 미치는지 분석하였다. 기존 연구와는 다르게 벤처기업의 내부역량을 측정함에 있어 자원의 양적인 측면보다는 질적인 측면에 초점을 맞추었고, 경영성과는 재무적 성과와 비재무적 성과로 구분하여 벤처기업에 대한 정부지원자금의 효과를 자세히 살펴보고자 하였다. 본 연구의 가설 검증을 위하여 중소벤처기업부에서 1999년부터 편제하고 있는 벤처기업정밀실태조사의 2017년 자료를 활용하였다. 연구 결과에 따르면 첫째, 정부지원자금 수혜경험은 벤처기업의 내부역량과 재무적 성과에 영향을 미치지 않았으나 기업의 비재무적 성과에는 영향을 미치는 것으로 나타났다. 그리고 비재무적 성과는 다시 기업의 재무적 성과에 영향을 미치는 것으로 나타났다. 둘째, 비재무적 성과에는 기술 가격 디자인 품질 경쟁력이 영향을 미치는 반면, 재무적 성과에는 가격 디자인 조직관리 마케팅 경쟁력이 영향을 미치는 것으로 나타났다. 마지막으로 성장단계에 따라 벤처기업의 내부역량과 경영성과에 차이가 있으나, 정부지원자금 수혜경험과 상호작용 효과에 따른 차이는 없는 것으로 나타났다.

• 한국지반환경공학회 논문집
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• 제12권7호
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• pp.23-29
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• 2011

산화슬래그는 토목분야에 널리 활용되어 왔으나 전기로 제강 환원슬래그는 거의 활용되지 않고 있는 실정이다. 따라서 토목 분야에서의 재활용 방법을 모색하기 위하여 환원슬래그의 성분을 분석하고 이를 바탕으로 토목현장에서 흔히 볼 수 있는 풍화토와 환원슬래그를 중량비 0% 내지 30%로 혼합한 후, 폐합시스템의 Lysimeter에서 1주, 2주, 4주의 주기로 환원슬래그 혼합토의 환경적 특성을 고찰하기 위한 시험을 수행하였다. 환원슬래그를 XRD, XRF를 이용하여 정성 정량적으로 분석한 결과, calcium silicate의 일종인 $Ca_2(SiO_4)$가 주요 결정 광물로 나타났다. 그리고 ICP-OES에 의한 중금속 분석결과 철강 생산 공정에 의한 영향으로 Al, Fe, Mn이 환원슬래그에 포함된 주요 중금속으로 확인되었다. 이를 폐기물관리법에 의한 오염물질 배출허용기준과 비교한 결과 중금속에 의한 오염가능성은 거의 없을 것으로 사료된다. 또한 환원슬래그 혼합토 침출수는 0% 혼합토에서 pH 6.9로부터 30% 혼합토에서 pH 10으로 측정되어 문제가 없음을 확인하였다. 침출수의 순환주기에 의한 pH 변화는 거의 없는 것으로 나타났다.

• Animal Bioscience
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• 제34권8호
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• pp.1392-1402
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• 2021

Objective: The growth rate of pigs is related to differentiation and proliferation of muscle cells, which are regulated by growth factors and expression of growth-related genes. Thus, the objective of this study was to establish optimal culture conditions for Jeju black pig (JBP) muscle cells and determine the relationship of various factors involved in muscle growth with the proliferation of JBP muscle cells. Methods: Muscles were taken from the femur skeletal muscle of JBP embryos. After isolation of the muscle cells, cells were cultured in a 6-well plate under four different culture conditions to optimize culture conditions for JBP muscle cells. To analyze proliferation rate of JBP muscle cells, these muscle cells were seeded into 6-well plates at a density of 1.5×10⁵ cells per well and cultured for 3 days. Western blot and quantitative real-time polymerase chain reaction were applied to verify the myogenic differentiation 1 (MyoD) expression and growth-related gene expression in JBP muscle cells, respectively. Results: We established a muscle cell line from JBP embryos and optimized its culture conditions. These muscle cells were positive for MyoD, but not for paired box 7. The proliferation rate of these muscle cells was significantly higher in a culture medium containing bFGF and epidermal growth factor + basic fibroblast growth factor (EGF+bFGF) than that without a growth factor or containing EGF alone. Treatment with EGF and bFGF significantly induced the expression of MyoD protein, an important transcription factor in muscle cells. Moreover, we checked the changes of expression of growth-related genes in JBP muscle cells by presence or absence of growth factors. Expression level of collagen type XXI alpha 1 gene was changed only when EGF and bFGF were added together to culture media for JBP muscle cells. Conclusion: Concurrent use of EGF and bFGF increased the expression of MyoD protein, thus regulating the proliferation of JBP muscle cells and the expression of growth-related genes.