• 제목/요약/키워드: quail.

검색결과 135건 처리시간 0.029초

QT35 세포주에서 제조합 에리스로포이에틴 생산을 위한 무혈청 배지의 개발 (Serum Free Medium Development for Recombinant Erythropoietin Production using Novel Cell Line (QT35))

  • 주형민;김병기;김선영;김태한;김태용
    • KSBB Journal
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    • 제13권3호
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    • pp.295-302
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    • 1998
  • Human Erythropoietin (EPO) gene is cloned in quail fibrosarcoma cell, QT35. Because molecular weight of EPO is similar to that of serum albumin, cell culture with serum containing medium makes purification of EPO very difficult. Using fractional factorial study, we have developed serum free medium for the recombinant QT35 cell lines, QT N4D4 and QT SY-IMP, which have cloned EPO with glutamine synthetase (GS) gene amplification system and with puromycin selective marker, respectively. Among the seven frequently used medium components, fibronectin, BSA, and EGF were the most important for EPO production. However, sufficient fibronectin supplement to the medium did not make any good attachment of QT35 to culture plate over 3 days. Therefore, to maximize EPO production, we attempted a medium-shift at confluence from serum containing medium to serum free medium(QT SFM6). Using the medium-shift protocol with QT SFM6, nearly the same productivity of EPO was achieved comparing with that without medium-shift. This result was true in both QT35 cell lines in three types of culture, i.e. T flask, microcarrier and roller bottle cultures.

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The Effects of Dietary Garlic Powder on the Performance, Egg Traits and Blood Serum Cholesterol of Laying Quails

  • Yalcin, Sakine;Onbasilar, Ilyas;Sehu, Adnan;Yalcin, Suzan
    • Asian-Australasian Journal of Animal Sciences
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    • 제20권6호
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    • pp.944-947
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    • 2007
  • This study was conducted to study the effects of dietary garlic powder on laying performance, egg traits and blood serum cholesterol level of quails. A total of three hundred quails (Coturnix coturnix japonica) aged nine weeks were used. They were allocated to 3 dietary treatments. Each treatment comprised 5 replicates of 20 quails. The diets were supplemented with 0, 5 and 10 g/kg garlic powder. The experimental period lasted 21 weeks. The addition of garlic powder did not significantly affect body weight, egg production, feed consumption, feed efficiency, egg shell thickness, egg albumen index, egg yolk index and egg Haugh unit. Adding 5 and 10 g/kg garlic powder to the laying quail diets increased egg weight (p<0.01). Egg yolk cholesterol and blood serum cholesterol concentration were reduced with garlic powder supplementation. The results of this study demonstrated that garlic powder addition had a significant cholesterol-reducing effect in serum and egg yolk without adverse effects on performance and egg traits of laying quails.

일본산메추리(Coturnix coturnix japonica)의 염색체 다형현상 (Chromosomal Polymorphism of Japanese Quail(Coturnix coturnix japonica))

  • 손시환
    • 한국가금학회지
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    • 제17권4호
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    • pp.275-280
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    • 1990
  • Constitutive heterochromatin의 염색체 다형현상에 대해 사람을 비롯하여 돼지, 생쥐, 말, 닭 등에서 보고된 바 있다. 본 연구에서는 일본산메추리의 C-band 다형체 뿐만 아니라 염색체의 형태적 다형체를 발견하여 이의 다형현상을 밝혔다. 일반적인 염색체 분석방법 및 C-banding 방법으로서 밝힌 3가지 염색체 다형체는 4번 염색체 +/- 동형체, +/- 이형체 및 -/- 동형체이다. 이와 같은 다형체들은 무작위 집단 내에서 일반적이고 지속적으로 나타나며 Mendel 법칙에 따른 유전양상을 보인다. 따라서 본 연구에서 밝힌 염색체 다형체들은 여러 세포유전학적 연구에 표식인자(chromosome marker)로서 유용하게 이용되어질 수 있을 것으로 생각된다.

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식용란의 플루오로퀴놀론계 합성항균제의 잔류에 관한 조사 (Surveys on the residual level of fluoroquinolones in eggs)

  • 고바라다;박성도;장미선;나호명;김용환
    • 한국동물위생학회지
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    • 제28권3호
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    • pp.235-243
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    • 2005
  • This surveies were carried out to investigate the residual levels of fluoroquinolones in chicken and quail eggs by bioassay and HPLC method. The eggs of 240 samples collected from market and farm in Gwangju Metropolitan city were examined from May to December in 2003. Residual antibiotic materials were detected from 47 samples of the 240 eggs by bioassay. Of the 240 eggs assayed, ciprofloxacin, danofloxacin, norfloxacin, ofloxacin, orbifloxacin and perfloxacin were not detected but enrofloxacin was detected from 5 samples in 228 chicken eggs and 1 sample in 12 quail eggs using HPLC with fluorescence detector by multi-residue method. 2 sample eggs in 6 sample which were detected by HPLC were not positive with bioassay. The average residual concentration of enrofloxacin was 0.494 mg/kg in 6 positive samples. The highest residual concentration of enrofloxacin was 1.83mg/kg.

Nicotinic acid 결핍이 메추리 혈청의 여러 대사물질 수준에 미치는 효과 (Effects of Nicotinic Acid Deficiency on the Levels of Various Metabolites in the Serum of Quail)

  • 이재혁;박인국
    • 한국동물학회지
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    • 제34권2호
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    • pp.203-208
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    • 1991
  • Nicotinic acid 결핍시 메추리는 심각한 체중의 감소를 보였으며, 심장 및 간의 무게도 약간 감소하였다. 포도당의 농도는 현저하게 증가하였으나 콜레스테롤, 알부민 그리고 총 단백질 양의 변화는 없었다. Glutamic oxaloacetate iransaminase 와 glutamic pyruvate transaminase의 활성은 증가하였으나 alkaline phosphatase와 LDH의 활성은 변화가 없었다. 혈청속의 아미노산중 tryptophan, tyrosine, aspartic acid, glutamic acid 등의 농도는 감소하였으나, arginine, histidine, lysine 등은 변화가 없었다.

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