• 대한수학회논문집
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• 제20권3호
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• pp.521-529
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• 2005

We prove that the dimension of the space of positive (bounded, respectively) solutions for the Schrodinger operator whose potential q is nonnegative on a graph with q-regular ends is equal to the number of ends (q-nonparabolic ends, respectively).

• 대한한의학원전학회지
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• 제31권1호
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• pp.79-93
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• 2018

Objectives : The climate changes in the natural realm displays pheonomena of excess and deficiency due to the principle of Yiyinyiyangzhiweidao. Here, overabundant qi arises due to the works of multiplication and insultation. When this overabundant qi is in force, the retaliating qi appears without fail to create a parallel. This is the Autonomous Equilibrium Mechanism found in the natural world. Studying this mechanism is deeply significant in understanding the mechanisms of diseases. Methods : The paper is written by reviewing the texts found in Huangdineijing's Chapters of Yunqi, which are $Q{\grave{i}}jiaobiandalun$, Wuchangzhengdalun, $Liuyuanzhengj{\grave{i}}dalun$, Zhizhenyaodalun, and Suwenliuqixuanzhumiyu. Results & Conclusions : The overabundance and retaliation in Five Circuits take the form of the restrained child of the Five Circuits takes revenge on the overabundant qi on behalf of his mother. The overabundance and retaliation in Six Qi take the form of rapid healing of Benqi which was in stagnation. Traditionally, overabundant qi is the only one in existence when the Five Circuits are in excess and the year of Hai yin wu wei you xu's regular transformation. During this time, retaliating qi does not exist. When Five Circuits are in deficiency and in the year of Si shen zi chou mao chen's pattern transformation, both the overabundant and deficiency qis exist. However, regardless of regular transformation or pattern transformation, overabundant qi and retaliating qi cannot exist at the same time. This seems to be the rational conclusion. There are some regulations that overabundance and retaliation follow. First, the strong and weak, and number of days coincide. Second, overabundance qi appear during the first half of the period when the qi of controling heaven is in place. During the later half of the period when the qi of terrestrial effect is in force, retaliation qi is the one that appears. Third, overabundance and retaliation does not end with one time. Rather, they will continue to repeat appearance and disappearance without any set pattern. Fourth, the overabundance and retaliation of the guest qi and dominant qi only has overabundance and no retaliation.

• Animal cells and systems
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• 제5권2호
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• pp.153-156
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• 2001

A key aspect of genomic research in the “post-genome era”is to associate sequence variations with heritable phenotypes. The most common variations in the human genome are single nucleotide polymorphisms (SNPs) that occur approximately once in every 500 to 1,000 bases. Although analyzing the phenotypic outcome of these SNPs is crucial to facilitate large-scale association studies of genetic diseases, detection of SNPs from an extended number of human DNA samples is often difficult, labor-intensive and time-consuming. Recent development in SNP detection methods using DNA microarrays and mass spectrophotometry has allowed automated high throughput analyses, but such equipments are not accessible to many scientists. In this study, we demonstrate that a simple PCR-based method using primers with a mismatched base at the 3'-end provides a fast and easy tool to identify known SNPs from human genomic DNA in a regular molecular biology laboratory. Results from this PCR amplification of specific alleles (PASA) analysis efficiently and accurately typed the Q576R polymorphism of human IL4 receptor from the genomic DNAs of 29 Koreans, including 9 samples whose genotype could not be discerned by the conventiona1 PCR-SSCP (single strand conformation polymorphism) method. Given the increasing attention to disease-associated polymorphisms in genomic research, this alternative technique will be very useful to identify SNPs in large-scale population studies.

• Molecules and Cells
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• 제41권5호
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• pp.413-422
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• 2018

Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.