• Title/Summary/Keyword: pyrimidine base

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Optimization of Culture Conditions for the Production of Pyrimidine Nucleotide N-Ribosidase from Pseudomonas oleovorans (Pseudomonas oleovorans의 pyrimidine nucleotide N-ribosidase의 생성 최적조건)

  • Yu, Tae-Shick
    • Journal of Life Science
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    • v.14 no.4
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    • pp.608-613
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    • 2004
  • Pyrimidine nucleotide N-ribosidase (pyrimidine 5'-nucleotide phosphoribo (deoxyribo) hydrolase/pyrimidine 5'-nucleoude nucleosidase, EC 3.2.2.10) directly catalyzes pyrimidine 5'-nucleotide to pyrimidine base and ribose (deoxyribo) 5-phosphate. In order to clarify the best nutritional conditions for the growth and the pyrimidine nucleotide N-ribosidase production of Pseudomonas oleovorans ATCC 8062 the effects of various nutrients such as different carbon and nitrogen sources were studied. For the both the growth and the enzyme production, 2% fumarate, 1.5% peptone, 5% corn steep liquor (CSL) and 1% ammonium chloride were excellent carbon and nitrogen sources, respectively. Optimum pH, temperature, and cultivation time for the enzyme production were 7.0, $28^{\circ}C$, and 48 h, respectively. The pyrimidine nucleotide N-ribosidase of P. oleovorans ATCC 8062 was not induced by UMP and its derivatives, and was constitutive enzyme.

The Effect of Antibiotics on the DNA Synthesis and Base Composition in Fungal Cells (진균류의 DNA 생합성 및 염기조성에 미치는 항생물질의 효과)

  • Park, Kyou-Yeon;Lee, Chong-Sam
    • The Korean Journal of Mycology
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    • v.22 no.4
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    • pp.366-377
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    • 1994
  • The base composition of DNA of Aspergillus phoenicis, Rhizopus acidus and Candida albicans treated with cycloheximide and nalidixic acid during the culture was analyzed to compare with the control. The contents of base in the DNA were inhibited by cycloheximide, 20.4% of adenine, 43.1% of thymine, 40.9% of cytosine, 35.3% of guanine, 32.2% of purine, and 42.7% of pyrimidine for A. phoenicis. In R. acidus, 34.2% of adenine, 42.1% of thymine, 38.0% of cytosine, 18.1% of guanine, 24.1% of purine and 40.0% of pyrimidine were depressed by cycloheximide. In the antibiotic treatment of C. albicans, 58.3% of adenine, 58.5% of thymine, 58.1% of cytosine, 42.4% of guanine, 46.8% of purine and 58.8% of pyrimidine were inhibited to compare with the control. The nalidixic acid treatments were showed that, in A. phoenicis 41.6% of adenine, 47.1% of thymine, 59.3% of cytosine, 46.3% of guanine, 45.6% of purine and 57.2% of pyrimidine were inhibited. When R. acidus was treated with nalidixic acid, 59.1% of adenine, 54.7% of thymine, 35.3% of cytosine, 37.4% of guanine, 45.9% of purine and 44.9% of pyrimidine decreased. In treatment of nalidixic acid, the content of DNA was depressed 60.1% of adenine, 68.6% of thymine, 60.7% of cytosine, 40.0% of guanine, 45.8% of purine and 63.5% of pyrimidine for C. albicans In the DNA synthesis of three fungal cells, cycloheximide and nalidixic acid treatments were analyzed obviously that the biosynthesis of pyrimidine was depressed than that of purine. Therefore, it was showed that the DNA contents in the various fungal cells were inhibited remarkably in nalidixic acid treatment than cycloheximide.

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Synthesis of Pyrimidine Nucleoside Analogues and Screening of Their Biological Effects (Pyrimidine Nucleoside 유도체들의 합성 및 약물학적 효능 검색)

  • 신혜순;이희주
    • Biomolecules & Therapeutics
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    • v.3 no.3
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    • pp.217-222
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    • 1995
  • Several acyclonucleoside analogues of pyrimidine base and N$^1$-derivatives of 5-fluorouracil have been synthesized and evaluated for their biological effects. When tested with in vitro Lekemia L1210 cells, the 5-fluorouracil derivatives exhibited slightly higher antitumor activity than the parent 5-fluorouracil. When tested against Herpes Simplex Virus type 1 and type 2 cultured in the Vero cell, the 5-fluorouracil derivatives showed weak antiviral activity.

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Antimetabolites in Nucleic Acid Biosynthesis (핵산 대사 길항제)

  • 장일무
    • YAKHAK HOEJI
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    • v.21 no.2
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    • pp.70-80
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    • 1977
  • 본고에서는 핵산 생합성과 Salvage patheway (Preformed Purine Utilization), De novo Pyrimidine Biosynthesis, Slavage patheway (Preformed Pyrimidine Utilization), Purine Base 및 Purine Nucleoside와 비슷한 구조를 가진 핵산 대사 길항제에 대한 내용으로 구성되어 있다.

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Purification and Characterization of Pyrimidine Nucleotide N-Ribosidase from Pseudomonas oleovorans

  • YU, Tae-Shick
    • Journal of Microbiology and Biotechnology
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    • v.15 no.3
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    • pp.573-578
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    • 2005
  • Pyrimidine nucleotide N-ribosidase (pyrimidine 5'-nucleotide phosphoribo(deoxyribo)hydrolase/pyrimidine 5'-nucleotide nucleosidase, EC 3.2.2.10) catalyzes the breakdown of pyrimidine 5'-nucleotide into pyrimidine base and ribose(deoxyribo)-5-phosphate. However, detailed characteristics of the enzyme have not yet been reported. The enzyme was purified to homogeneity 327.9-fold with an overall yield of $6.1\%$ from Pseudomonas oleovorans ATCC 8062. The enzyme catalyzed cytidine monophosphate (CMP) and uridine monophosphate (UMP), but not adenosine monophosphate (AMP) and guanosine monophosphate (GMP). The enzyme optimally metabolized CMP at pH 6.0 and UMP at around 8.5, and the optimum temperature for the overall enzyme reaction was found to be $37^{\circ}C$. The $K_m$ values of the enzyme for CMP (at pH 6.0) and UMP (at pH 8.5) were 1.6 mM and 1.1 mM, respectively. AMP, deoxyCMP, and deoxyUMP were very effective inhibitors of the reaction. Double-reciprocal plots obtained in the absence and in the presence of AMP revealed that this inhibitory effect was of the mixed competitive type with respect to the breakdown of CMP and of the noncompetitive type with respect to the breakdown of UMP. In the presence of AMP, the enzyme followed sigmoid kinetics with respect to each substrate.

Synthesis and Antimicrobial Screening of Pyrimidine Annulated Dihydropyrano[2, 3-c]pyrazole Derivatives

  • Hegde, Hemant;Ahn, Chuljin;Waribam, Preeti;Shetty, Nitinkumar S.
    • Journal of the Korean Chemical Society
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    • v.62 no.2
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    • pp.87-92
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    • 2018
  • A series of pyrimidine annulated dihydropyrano[2, 3-c]pyrazole derivatives were synthesized and screened for their antimicrobial activity. The precursor, dihydropyrano[2, 3-c]pyrazole was synthesised under catalyst free condition using PEG-400 as reaction medium which facilitated improved yield compared to base catalyzed reaction. Wide scope of substrates, simple workup procedure and high yield even in the absence of catalyst are the major highlights of the protocol. Dihydropyrano[2, 3-c]pyrazoles on condensation with formic acid formed pyrimidine annulated dihydropyrano[2, 3-c]pyrazole derivatives. All the products are characterized using FTIR, $^1H-NMR$ and $^{13}C-NMR$ spectroscopic techniques. The molecules have shown good to moderate activity as antimicrobial agents when compared to the standard drug ciprofloxacin.

Synthesis and Physicochemical Properties of Polynucleotide Analogues Containing Pyrimidine Bases

  • Han, Man Jung;Kim, Gi Ho;U, Gyeong Su;Jang, Ji Yeong;Park, Yeong Dong
    • Bulletin of the Korean Chemical Society
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    • v.21 no.3
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    • pp.321-327
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    • 2000
  • Polynucleotide analogues containing pyrimidine (uracil and thynime) bases, $poly[(1'{\beta}-uracil-1-yl-2'.5'-dideoxy-D-glycero-pent-4'-enofuranose)-alt-(maleic$ acid)] (12) and $poly[(1'-{\beta}-thymin-1-yl-2'5'-dideoxy-D-glycero-pent-4'-enofuranose)-alt-(maleic$, acid)] (15), were synthesized by the altermating copolymerization of relevant nucleosied derivatives and maleic anhydride, and the subsequent hydrolysis. The polymers had quite similar structures to the natural polymes and were soluble in water, They showed high hypochromicities up to 49% and excimer fluorescence due to the base stacking, and polyelectrolyte behavior. Since the polymers had compact structrures, depyrimidinations, the release of pyrimidine bases from the polymer backbone, occurred in aqueous solutions with higher rates compared with those of the natural polymers.

Base Catalysed Pyrimidine Synthesis Using Microwave

  • Kidwai, M.;Rastogi, S.;Saxena, S.
    • Bulletin of the Korean Chemical Society
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    • v.24 no.11
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    • pp.1575-1578
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    • 2003
  • An environmentally benign approach for the synthesis of 2-substituted-4,6-diaryl pyrimidines using inorganic solid supports for its catalytic role as well as an energy transfer medium is described. The methodology eliminates the usage of solvent during the reaction. The reaction time is brought down from hours to minutes along with yield enhancement. The rate enhancement and high yield is attributed to the coupling of solvent free conditions with microwaves. Further, the role of base is studied in the reaction and it is concluded that microwave assisted basic alumina catalysed reaction is the best in terms of catalysis as well as reaction time and yield.

Synthesis of D-1,3-Dioxolane and D-1,3-Oxathiolane Pyrimidine Nucleosides (D-1,3-Dioxolane 및 D-1,3-Oxathiolane 피리미딘 뉴크레오사이드의 합성)

  • Hong, Joon-Hee;Cha, Ma-Rie;Shin, Seong-Eun;Choi, Bo-Gil;Chung, Byung-Ho;Kim, Joong-Hyup;Lee, Chong-Kyo;Chung, Won-Keun;Chun, Moon-Woo
    • YAKHAK HOEJI
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    • v.38 no.6
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    • pp.703-711
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    • 1994
  • We synthesized D-1,3-dioxolanyl acetate from D-mannose using Frazer-Reide reaction and D-1,3-oxathiolanyl acetate from either D-mannose or D-galactose in good yields. These acetates were conjugated with various disilylated pyrimidine base using trimethylsilyl trifluoromethane sulfonate as a Lewis acid catalyst to obtain 32 different D-nucleosides.

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Synthesis and Antiviral Evaluation of Novel Pyrimidine Thioapionucleosides (신규 피리미딘 티오에피오 뉴크레오사이드의 합성 및 항바이러스 약효검색)

  • Lee Rae-Sang;Hong Joon-Hee;Ko Ok-Hyun
    • YAKHAK HOEJI
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    • v.50 no.2
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    • pp.65-69
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    • 2006
  • Novel 4'-hydroxymethyl branched thioapiosyl nucleosides were synthesized in this study. The introduction of hydroxymethyl group in the 4'-position was accomplished by a [3,3]-sigmatropic rearrangement. Thioapiosyl sugar moiety was constructed by sequential ozonolysis, reduction and cyclization. The pyrimidine nucleosidic bases (uracil, 5-fluorouracil, 5-iodouracil, 5-chlorouracil, 5-bromouracil) were efficiently coupled by Vorbruggen glycosyl condensation procedure (per-silyated base and TMSOTf). The antiviral activities of the synthesised compounds were evaluated against the HIV-1, HSV-1, HSV-2 and EMCV 5-Iodouracil 18 showed weak antiviral activity against HSV-1 $(EC_{50}=30.7{\mu}M)$.