• Bulletin of the Korean Chemical Society
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• v.32 no.5
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• pp.1495-1499
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• 2011

A solution processable polymer was synthesized, by incorporating pyrene groups into the backbone of the polyaniline chain, and used as an emissive layer in an organic light emitting diode. The polyaniline base was reacted with acid chloride of pyrene butyric acid to form pyrene-functionalized polyaniline chains. The source of pyrene moiety was acid chloride of pyrene butyric acid. The formation of polymer from acid chloride of pyrene butyric acid and polyaniline was confirmed by the FTIR and $^1H$-NMR spectroscopy. Differential scanning calorimetry revealed high glass transition temperature of 210 $^{\circ}C$. Due to the presence of pyrene moieties in the backbone, the polyaniline synthesized in the present study is solution processable with light emitting property. The photoluminescence spectrum of the polymer revealed that emission lies in the blue region, with a peak at 475 nm. The light emitting device of this polymer exhibits the turn-on voltage of 15 V.

• Journal of Korean Society for Atmospheric Environment
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• v.5 no.2
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• pp.12-20
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• 1989

Distribution of benzo(a)pyrene (BaP) in Seoul surface soils was investigated with a simple micro-analytical method consisting of ultrasonic extraction, one-dimensional dual band thin-layer chromatographic separation (TLC) and spectrofluorometric determination. The TLC was done in the following condition: thin-layer plate; Kieselguhr G/Acetylated cellulose, Developer; 1st; ether, 2nd; methanol-ether-water (4:4:1, V/V). The results thus obtained were as follows; 1. All the samples collected from various areas were contained detectable amount of benzo(a)pyrene. The range and average of benzo(a)pyrene contents in Seoul areas are 0.20 $\sim$5.90ppm and 1.01 ppm, respectively. 2. Benzo(a)pyrene contents in soils obtained from commercial areas were much higher than those in industrial and residential areas. The contents range in commercial, industrial, and residential areas are 0.31 $\sim$ 5.90 ppm, 0.36 $\sim$ 1.22 ppm and 0.20 $\sim$ 0.67 ppm, respectively. 3. Benzo(a)pyrene contents in soils from major roads were far higher than those from side or park roads. The ranges of benzo(a)pyrene contents in major, park and side road are 0.40 $\sim$ 5.9 ppm, 0.20 $\sim$ 0.70 ppm, 0.31 $\sim$ 1.30 ppm, respectively. These findings suggest strongly that surface soils in Seoul city are polluted by benzo(a)pyrene probably emitted automobiles.

• Environmental Analysis Health and Toxicology
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• v.20 no.1
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• pp.23-28
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• 2005

PAH 위해성 평가의 생체지표 개발을 위하여, benzo[a]pyrene을 인체 간암 세포주인 HepG2세포에 처리하여 암 억제 단백질인 p53 및 관련 단백질의 발현 양상에 대하여 연구하였다. HepG2 세포의 생존력은 benzo[a]pyrene을 노출시킨 군에서 농도가 증가할수록 감소하였다. p53과 인산화 p53의 발현 양상은 benzo[a]pyrene 농도 의존적으로 증가하는 경향을 보였으며, 반면에 아세틸화 p53은 benzo[a]pyrene의 농도가 증가할수록 감소하는 경향을 나타내었다. 세포 주기 조절에 관련된 p21 단백질은 화학 물질 처리에 의해서 p53과 마찬가지로 증가하였으나, CdK4와 Rb 단백질의 발현에는 변화가 없었다. 상관분석 결과 Benzo[a]pyrene 노출, 세포 생존력, p53, 인산화 p53, p21이 서로 높은 상관성을 보였다. 본 연구의 결과는 p53 단백질의 축적이 benzo[a]pyrene 독성에 있어 매우 중요한 현상이며, 이는 선택적인 지표와 함께 p53 이 benzo[a]pyrene과 같은 PAH 계열의 물질의 위해성 평가를 위한 민감한 생체 지표로써 개발될 수 있음을 시사한다.

• Journal of Environmental Science International
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• v.16 no.3
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• pp.247-253
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• 2007

Polycyclic aromatic hydrocarbons (PAHs) are a major concern because of their potential mutagenic and carcinogenic risks to human beings. One of these harmful, yet commonly observed PAHs is pyrene. Pyrene is one of the 16 PAHs listed by the United States Environmental Protection Agency as priority pollutants. The purposes of this research are to develop a method of pretreatment for PAH contaminants prior to a typical biological treatment and to demonstrate the biodegradablity of these compounds. Since pyrene is non-polar, hexane was chosen as a solvent to effectively dissolve pyrene. Pyrene solutions were treated with ozone, as it has hish oxidation capacity and electrophilic character. The intermediates and byproducts of pyrene were dissolved in alkaline water at pH 11.4 and neutralized to test for $BOD_5$, COD, and toxicity. These solutions were further ozonated and assessed of biodegradability. The first-order rate constant to was found to be between $0.121day^{-1}$ and $0.081 day^{-1}$, depending on the duration of reozonation. The $BOD_5/COD$ ratio was found to 0.66. The toxicity test showed that after 10 min of reozonation time, the byproducts and intermediates of pyrene were within the lion-toxic range of ${\pm}10%$ inhibition for E-Coli bacteria.

• Food Science of Animal Resources
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• v.31 no.6
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• pp.960-965
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• 2011

The content of benzo[a]pyrene from 69 smoked meat products commonly consumed in Korean food market was analysed with high performance liquid chromatography. Smoked meat products including smoked chicken, pork, turkey and duck were saponified, extracted and cleaned up to analyze the benzo[a]pyrene content. As a result of analysis from smoked meat products, the mean benzo[a]pyrene content was 0.42 ${\mu}g$/kg and the highest content of benzo[a]pyrene was 2.87 ${\mu}g$/kg detected in smoked chicken product. All somked meat products contained benzo[a]pyrene below the limit regulated by Korean Food and Drug Administration (KFDA). Exposure assessment of benzo[a]pyrene from smoked meat products ingestion was calculated by using National Health and Nutrition Survey (NHNS). The estimated lifetime average daily intake of benzo[a]pyrene was 0.187 ng/kg bw/d. Margin of exposure of benzo[a]pyrene was ranged from 1,657,754 to 3,957,219.

• The Korean Journal of Food And Nutrition
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• v.22 no.2
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• pp.199-204
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• 2009

For the purpose of developing a safe & hygienic manufacturing method to acquire low levels of benzo(a)pyrene in black and red ginseng products, this study investigated the effects of steam- and dry-processing temperatures on benzo(a)pyrene production in ginseng. By the red ginseng with a fix dry-process temperature of $50^{\circ}C$ and setting the steam-process temperature between $80{\sim}120^{\circ}C$, an extremely small amount(0.1 ppb) of benzo(a)pyrene was produced, indicating there was no relationship between the steam-temperature and benzo(a)pyrene production. On the other hand, when the red and black ginseng were steamed at the fixed temperature of $100^{\circ}C$ and dried at various temperatures between $50{\sim}120^{\circ}C$, the amount of benzo(a)pyrene produced was closely connected with the dry-temperature, and increased with higher drying temperatures. Upon repeating the steam and dry process nine times, in which the steam-temperature was set at $100^{\circ}C$ and the dry-temperature at $50^{\circ}C$, higher amount of benzo(a)pyrene were produced in red and black ginseng, respectively, with increasing steam- and dry-processing time. However, the level of benzo(a)pyrene still remained extremely small(below 0.12 ppb), showing a maximum amount in the black ginseng that was steamed and dried nine times. This suggests that the fine root of ginseng may be carbonized by increasing the number of times it is steam- and dry-processed. From the above results, this study determined that the optimum temperatures for manufacturing red and black ginseng products with safe levels of benzo(a)pyrene would be a temperature between 80 and $120^{\circ}C$ for steaming and a temperature less than $50^{\circ}C$ for drying.

• Rapid Communication in Photoscience
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• v.3 no.4
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• pp.76-78
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• 2014

Fluorescent nucleic acids were prepared utilizing the polymerase extension (PEX) reaction to incorporate fluorescent molecules. 2'-Deoxyuridine triphosphate (dUTP) derivatives possessing pyrene molecules as fluorophores were synthesized using the aqueous-phase Sonogashira coupling between 5-Iodo-dUTP and acetylene-linked pyrene molecules. The incorporation of the pyrene (Py)-labeled deoxyuridine triphosphates (PyU) into DNA by polymerase was evaluated by polyacrylamide gel electrophoresis, demonstrating that the PyU can work as a good substrate for the PEX reaction. The fluorescent properties of the functionalized DNA prepared by the PEX reaction were characterized by steady-state fluorescence measurements. The Py-conjugated DNA showed typical emission spectra of the pyrene, and the DNA with two pyrene molecules connected to each other by a diethylene glycol linker exhibited a broadened emission attributed to the electronic interaction between the Py molecules.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.4
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• pp.564-569
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• 2014

This study was conducted to reduce benzo(a)pyrene in sesame oil by addition of several kinds of absorbents (active carbon, diatomaceous earth, kaolin, acid clay, perlite, and silicate). Sesame oil containing 4.1 ppb benzo(a)pyrene was stirred with 0.2% (w/w) several kinds of adsorbents at $40^{\circ}C$ for 30 min. Active carbon resulted in the highest reduction of benzo(a)pyrene in sesame oil among the investigated adsorbents, and decolorization was observed only by using silicate. Reduction of benzo(a)pyrene was optimized by controlling amount the of active carbon, stirring time, and stirring temperature. Futher, 4.1 ppb benzo(a)pyrene in sesame oil was reduced by up to 0.91 ppb by adding 0.5% (w/w) active carbon and stirring for 30 min at $70^{\circ}C$. Optimized conditions were applied to sesame oil (2.14~4.11 ppb) purchased from a Gyeonggi traditional market, and benzo(a)pyrene in sesame oil was reduced by up 0.43~0.86 ppb.

• Food Engineering Progress
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• v.13 no.3
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• pp.211-215
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• 2009

Concentrations of benzo[$\alpha$]pyrene in edible oils from Korean market were evaluated by high performance liquid chromatography. Benzo[$\alpha$]pyrene known of the carcinogenic polycyclic aromatic hydrocarbons(PAHs), has been found at variable concentrations in several foods. This is associated with several factors during the process including contaminated raw materials, exposure of environment, and procedure of process or cooking. The levels of benzo[$\alpha$]pyrene were ranged from 0.5 to 1.4 $\mu$g/kg in virgin olive oil. Benzo[$\alpha$]pyrene contents in refined and virgin olive oil, sesame oil, soybean oil, corn oil, sunflower oil, safflower oil, and processed oil were 0.6-1.0 $\mu$g/kg, 0.9-1.3 $\mu$g/kg, 0.6-3.3 $\mu$g/kg, 0.5-1.1 $\mu$g/kg, 1.2-1.7 $\mu$g/kg, 1.0-2.1 $\mu$g/kg, and 1.0-1.4 $\mu$g/kg, respectively.

• Journal of Korean Society for Atmospheric Environment
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• v.3 no.2
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• pp.11-17
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• 1987

Atmospheric particulate matter (A.P.M.) was collected on quartz fiber filters from March 1985 to February 1986 at Chung-Ang University according to particle size using Andersen high-volume air smapler, and benzo (a) pyrene concentration in these particulates were analyzed by high performance liquid chromatography. The annual arithmetic mean concentration of A.P.M. was 115.50$\mug/m^3$. The annual arithmetic mean concentrations of coarse particles and fine particles in A.P.M. were 52.54$\mum/m^3$ and 62.96$\mum/m^3$ respectively. THe annual arithmetic mean concentration of benzo(a)pyrene in A.P.M. was 1.44$ng/m^3$. THe annual arithmetic mean concentrations of benzo(a)pyrene in coarse particles and fine particles were 0.05 $ng/m^3$ and 1.39 $ng/m^3$ respectively. Thus, the concentration of benzo(a)pyrene showed maldistribution of 96.53% in fine particle. A.P.M. showed wide fluctuation according to the season. The concentration of A.P.M. was lowest in summer and high in spring and winter. Coarse and fine particle concentrations in A.P.M. were highest in spring and winter, respectively. The concentrations of benzo(a)pyrene was highest in winter and lowest in summer. The concentrations of benzo(a)pyrene in fine and coarse particles were highest in winter and spring, respectively.