• 한국산림과학회지
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• 제88권3호
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• pp.364-373
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• 1999

이 연구는 전나무림과 잣나무림에서 산림시업(山林施業)(간벌 및 가지치기)이 토양(土壤)의 물리성(物理性) 및 수질변화(水質變化) 등에 미치는 영향(影響)을 파악함으로써 산림시업(山林施業)에 따른 산림(山林)의 수질정화기능(水質淨化機能)을 평가(評價)하기 위한 기초자료(基礎資料)를 제공(提供)하기 위하여 실시하였다. 1996년 3월부터 5월까지 산림시업을 한 광릉시험림 31, 33임반내 전나무림과 잣나무림 유역에서 1998년 3월 1일부터 1998년 8월 4일까지 임분특성(林分特性), 토양(土壤)의 물리적(物理的) 특성(特性), 그리고 강우(降雨), 임내우(林內雨)(수관통과우(樹冠通過雨), 수간류(樹幹流)), 토양수(土壤水), 계류수(溪流水)의 수질동태(水質動態)를 분석(分析)하였다. 산림시험 후 약 2년 6개월이 경과된 1998년 9월 현재, 전나무림과 잣나무림에서 임목의 평균수고(平均樹高), 평균흉고직경(平均胸高直徑)은 시업구가 비시업구 보다 각각 1.8m, 0.6m, 4.7cm, 1.4cm 더 성장하였으며, 시업구는 비시업구 보다 A층 토양에서의 조공극율(粗孔隙率), 총공극율(總孔隙率)은 증가하였으나, 토양(土壤)의 가비중(假比重)은 감소하여 전나무림과 잣나무림에서는 산림시업(山林施業)에 따라 산림토양(山林土壤)의 강수침투구조(降水浸透構造)는 개선(改善)되는 것으로 나타났다. 또한, 전나무림과 잣나무림에서 산림시업(山林施業)으로 임우내(林內雨) 및 토양수(土壤水)의 완충효과(緩衝效果)가 증대(增大)되는 것으로 분석(分析)되었다.

• 산업식품공학
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• 제15권1호
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• pp.22-27
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• 2011

본 연구에서는 강원도 춘천지역에서 발생하는 환경 오폐수를 시료로 하여 미생물을 혼합하여 제조한 수질정화제를 실제 하천과 호수의 오염환경과 유사한 배양기내에서 생물학적반응을 시켜오염물질의정화정도를측정하고평가하였다. 사용된 미생물은 식물성 유산균(vegetable Lactobacillus fermentum), 효모(Saccharomyces cerevisiae)와 고초균(Bacillus subtilis)이다. 두가지의미생물혼합제를투입하여정화정도를 실험하였다. 실제 하천에 투입하여 정화되는 과정과 유사하게 반응을 시키고자 반응 온도는 $21({\pm}3)^{\circ}C$로 하였으며 완전교반을 진행하였다. 사용한 시료의 오폐수의 오염도와 미생물에 의한 정화정도를 평가하기 위하여 총질소 함량과 총인 함량을 측정하였다. V.L.F.와 S.C.의 혼합물로 구성된 혼합제의 경우 총질소함량을 감소시키는데 매우 효과적임을 보여주었으며, V.L.F., S.C.,와 B.S.로 구성된 혼합제의 경우 그 총인의 함량을 저감화하는데 효과적임을 알 수 있었다. 본 실험에서 얻어진 실험 데이터를 통하여 V.L.F.와 S.C.의 혼합제가 오폐수의 정화반응에서 나타나는 반응상수값을 도출하였으며 그 값은 0.178 $day^{-1}$이었다. 본 연구의 결과를 바탕으로 저감화하고자 하는 성분에 따라 미생물의 종류와 그 혼합비를 조절하는 최적화과정이 필수적임을 알 수 있었으며 또한 곡선적합으로 도출한 반응상수값은 미생물을 이용하여 호수와 하천의 정화정도를 simulation함에 필요한 실험인자로서 응용이 가능하게 되었다.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.543-551
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• 2001

The vascular endothelial growth factor (VEGF), or VEGF-A, is intimately involved in both physiological and pathological forms of angiogenesis. VEGF-A is now recognized as the founding member of a family of growth factors that has expanded to include VEGF-B, VEGF-C, VEGF-D, VEGF-E, and placental growth factor (PIGF). This family of cytokines binds differentially to at least three receptor tyrosine kinases, however, the extent to which family members other than VEGF-A contribute to physiological and pathological angiogenesis remains unclear. Issues that are of relevance include uncertainty regarding the consequences of signaling through VEGF - RI in particular, and the ability of some family members to heterodimerize, leading to the possibility ofheterodimeric receptor complexes. Structural characterization is one approach that can be used to address these issues, however, the vast majority of previous structure-function studies have only focused on VEGF-A. While these studies may provide some clues regarding the structural basis of the interaction of other family members with their receptors, studies using the ligands themselves are clearly required if highly specific interactions are to be revealed. With the recent progress toward refolding and purifying substantial' quantities of other VEGF family members, such structural studies are now possible. Here, these ~ssues are addressed with a particular emphasis on VEGF-B and its receptors.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.509-516
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• 1998

A plasmid vector, pXGPRMATG-lac-Tgx, was developed for overproduction of $\beta$-galactosidase in Escherichia coli using the genetic components of groEx, a heat-shock gene cloned from symbiotic X-bacteria in Amoeba proteus. The vector is composed of intragenic promoters P3 and P4 of groEx, the structural gene of lac operon, transcription tenninator signals of lac and groEx, and ColEl and amp'of pBluescript SKII. The optimized host, E. coli DH5$\alpha$, transfonned with the vector constitutively produced 117,310-171,961 Miller units of $\beta$-galactosidase per mg protein in crude extract. The amount of enzyme in crude extract was 53% of total water-soluble proteins. About 43% of the enzyme could be purified to a specific activity of 322,249 Miller units/mg protein after two-fold purification, using two cycles of precipitation with ammonium sulfate and one step of gel filtration. Thus, the expression system developed in this study presents a low-cost and simple method for purifying overproduced $\beta$-galactosidase in E. coli.

• 환경영향평가
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• 제14권4호
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• pp.147-155
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• 2005

This study was designed to investigate the effect of plant as a botanical air purification on the indoor pollution by formaldehyde. Three indoor plants such as Dracaena marhginata, Spathiphyllum and Dracaena reflexa, were placed in the artificially contaminated reactor under laboratory condition. Both plant and soil effects on removal of formaldehyde from contaminated indoor air were observed. Reductions in the formaldehyde levels appeared to have been associated with soil medium factors as well as plant factors. The effect of soil on formaldehyde reduction was high in the early stage of the experiment and the results suggest that sorption could be more important factor than microbial degradation in the initial dissipation of contaminants in the soil. It was suggested that the effect of plant on formaldehyde reduction might be related to the plant species, total leaf surface area of plant, degree of contribution of soil medium, and exposed concentration level. The results of this study showed that air purification using plants is an effective means of reduction on indoor formaldehyde level, though, utilization of soil media with high sorption capacity and/or supplementary purifying aids were also suggested when the source is continuous or exposed concentration level is high.

• 대한기계학회논문집A
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• 제28권12호
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• pp.1990-1996
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• 2004

This paper presents a nonlinearly modulated digital actuator (NMDA) for producing nano-precision digital stroke. The NMDA, composed of a digital microactuator and a nonlinear micromechanical modulator, purifies the stroke of the digital actuator in order to generate the high-precision displacement output required for nano-positioning devices. The function and concept of the nonlinear micromechanical modulator are equivalent to those of the nonlinear electrical limiters. The linear and nonlinear modulators, having an identical input and output strokes of 15.2${\mu}{\textrm}{m}$ and 5.4${\mu}{\textrm}{m}$, are designed, fabricated and tested, respectively. The linear and nonlinear modulators are linked to identical digital actuators in order to compare the characteristics of the linearly modulated microactuator (LMDA) and NMDA. In addition, an identical linear modulator is attached to the output ports of LMDA and NMDA. The NMDA shows the repeatability of 12.3$\pm$2.9nm, superior to that of 27.8$\pm$2.9nm achieved by LMDA. When the identical linear modulator is connected to LMDA and NMDA, the final modulated output from NMDA shows the repeatability of 10.3$\pm$7.2nm, superior to that of 15.7$\pm$7.7nm from LMDA. We experimentally verify the displacement purifying capability of the nonlinear mechanical modulator, applicable to nano-precision positioning devices and systems.

• 기본간호학회지
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• 제23권4호
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• pp.402-410
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• 2016

Purpose: Personal protective equipment (PPE) is critical to protect healthcare workers from pandemic outbreaks. This study was designed to identify nurses' knowledge, attitude and perceptions on PPE. Methods: Data were collected from 154 nurses working at a tertiary general hospital, where positively diagnosed and suspected patients were accepted and admitted during the Middle East Respiratory Syndrome Coronavirus outbreak in 2015. The PPE tool consists of 20 items for knowledge, and 5 each for attitudes and perceptions. Results: Overall, knowledge for PPE was higher than moderate (76.95/100), but there was a lack for items related to powered air purifying respirator (PAPR). For attitudes, willingness to work in pandemic outbreaks was the most positive (4.04/5.00). Goggles and PAPR were perceived as the most uncomfortable barriers to work. There was no correlation between knowledge, attitude and perceptions, despite a strong positive correlation between attitude and perceptions. PPE training was identified as a factor to improve knowledge and attitudes on PPE. Conclusion: To respond to pandemic influenza, nurses need to expand their knowledge on PPE up to Level C, and be updated through regular training. Study findings suggest that repetitive studies targeting nurses and other healthcare workers at various hospital settings are necessary.

• Journal of Advanced Marine Engineering and Technology
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• 제34권1호
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• pp.84-91
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• 2010

The centrifugal oil purifier is used in ships for purifying the engine lubrication oil. The momentum needed for the rotation of the cylindrical chamber is obtained by jet injections. The dust particles in the oil are separated by the centrifugal forces moving to the inner wall of the rotating cylindrical chamber body. The dust particles are eliminated when the particles are adsorbed onto the surface of the inner wall of the chamber body. The flow characteristics and the physical behaviours of particles in this centrifugal oil purifier have been investigated numerically and the filtration efficiencies have been evaluated. For the calculations, a commercial code has been used and the SST k-${\omega}$ turbulence model has been adopted. The MRF (Multiple Reference Frame) method has been introduced to consider the rotating effect of the flows. Under various variables, such as particle size, particle density and rotating speed, the filtration efficiencies have been evaluated. It has been verified that the filtration efficiency is increased with the increments of the particle size, the particle density and the rotating speed of the cylindrical chamber.

• 한국환경과학회지
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• 제15권2호
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• pp.133-139
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• 2006

Soil biofiltration is an environmentally-sound technology for elimination of VOCs, odorous and NOx compounds from a low concentration, high volume waste gas streams because of its simplicity and cost-effectiveness. This study was performed to evaluate effect of removal of gaseous NOx using a soil and a yellow soil. Over $60\%\;and\;48\%$ of NOx from a soil and a yellow soil was removed at the inlet NO concentrations of $423\~451$ppb, respectively. The bio-filter using a soil media was capable of purifying NOx with a different natural processes. Although some of the processes are quite complex, they can broadly be summarized as adsorption into soil pore water, and biochemical transformations by soil bacteria. When the filteration bio-reactor was applied to a soil and a yellow soil, effective NOx removal was obtained for several times and months. These results show that a soil biofilter can be of use as an alternative advanced NOx treatment system.

• 한국환경과학회지
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• 제16권2호
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• pp.197-202
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• 2007

Formaldehyde is a typical indoor air pollutant that has numerous adverse health problems in modern living conditions. Phytoremediation that use plants to remove contaminants from polluted media can be applied to improve indoor air quality. Two sets of experiments; 1) two rooms in newly built auditorium and 2) a bed room in 2-year-old apartment; were performed to investigate plant effects on indoor formaldehyde concentration. It was observed from the experiments that plant can help decontaminating formaldehyde at low concentration level (0.1 ppm) but the effects decreased considerably at hish concentration (1ppm). The purification effects of indoor plant also showed the periodic pattern due to its physiological activity. More purification was observed as increasing plant density in the bed room but the formaldehyde concentration returned the original concentration level in two days after removing plants. It was suggested from the results that air purification using plants is an effective means of reduction on indoor formaldehyde level, though, reduction of source is highly desirable when the concentration level is high. The results also suggest that introducing supplementary purifying aids and/or efficient ventilation could be considered due to periodic removal pattern of plant.