• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.521-524
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• 2003

An effective purification method was developed for producing Homoharringtonine (HHT), to guarantee high purity and yield from Cephalotaxus koreana. This process was a simple and efficient procedure, for the isolation and purification of HHT form the biomass of Cephalotaxus koreana, consisting of extract, adsorbent treatment, precipitation and followed by a chromatography. The extraction, adsorbent treatment and precipitation in pre-purification process allows for rapid and efficient separation of HHT from many compound and dramatically increases the yield and purity of crude HHT for HPLC purification steps compared to alternative processes. This purification processes serves to minimize solvent usage, size, and complexity of the operations for HHT purification.

• KSBB Journal
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• 제21권1호
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• pp.1-10
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• 2006

식물세포배양으로부터 항암제 paclitaxel의 생산을 위해 회수 및 정제는 산업적 공정에 있어서 필수적이다. 본 총설은 식물세포배양으로부터 고순도, 고수율의 paclitaxel 생산을 위한 대량 회수 및 정제 방법을 기술하고자 한다. 또한 이러한 분리 및 정제 공정은 추출, 전 처리, 정제, 제품화 단계를 총괄하여 최종 제품의 요구조건들 즉, 순도, 잔류용매, 제품형태, 불순물 함량, 엔도톡신 함량 등을 충족시킬 수 있도록 최적화되어야 한다. 이러한 관점에서 본 총설은 산업화 단계에서의 의약품 생산 및 품질관리에 상당히 유용하게 활용될 수 있을 것으로 판단된다.

• KSBB Journal
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• 제15권4호
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• pp.342-345
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• 2000

식물세포배양으로부터 peroxidase를 대량 생산하기 위한 분리/정제 공정 으로서 세포를 파쇄하고 크로마토그래피 전처리로서 활성백토를 적용하였다. 활성백토는 미세한 세 포 조각 뿐만 아니라 여려가지 불순불을 선택적으로 흡착 하는 성질을 나타내었으며, 이를 통하여 효과적으로 정제 공정을 진행할 수 있었다 흡착을 통한 전처리 후 한외여 과장치를 이용하여 농축을 실시 하였으며, DEAE-Sepharose F FF를 이용한 크로마토그래피를 통해 정제가 이루어졌다. 활성을 나타내는 용액을 탈염, 농축 후 동결 건조하였다. 이 공정은 상업화에 필요한 대량 정제를 가능하게 하였으며 수율과 정제비용 측면에서 상당히 경제적인 공정임을 입증하였다, 활성백토를 이용한 흡착공정은 다른 효소 및 단백질의 경제적인 대량정제에 적용될 수 있으리라 기대된다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.317-320
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• 1997

In the water purification plant, chemicals are injected for quick purification of raw water. It is clear that the amount of chemicals intrinsically depends on the water quality such as turbidity, temperature, pH and alkalinity etc. However, the process of chemical reaction to improve water quality by the chemicals is not yet fully clarified nor quantified. The feedback signal in the process of coagulant dosage, which should be measured (through the sensor of the plant) to compute the appropriate amount of chemicals, is also not available. Most traditional methods focus on judging the conditions of purifying reaction and determine the amounts of chemicals through manual operation of field experts or jar-test results. This paper presents the method of deriving the optimum dosing rate of coagulant, PAC(Polymerized Aluminium Chloride) for coagulant dosing process in water purification system. A neural network model is developed for coagulant dosing and purifying process. The optimum coagulant dosing rate can be derived the neural network model. Conventionally, four input variables (turbidity, temperature, pH, alkalinity of raw water) are known to be related to the process, while considering the relationships to the reaction of coagulation and flocculation. Also, the turbidity in flocculator is regarded as a new input variable. And the genetic algorithm is utilized to identify the neural network structure. The ability of the proposed scheme validated through the field test is proved to be of considerable practical value.

• 산업기술연구
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• 제31권A호
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• pp.135-145
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• 2011

This study was conducted to analyze the cost effectiveness in line with total phosphorus standard enforcement of public sewage treatment facilities. The additional cost for the total phosphorus removal process was calculated to analyze the cost effectiveness of the advanced water purification process. The analysis results showed that the T-P removal by coagulation sedimentation was more efficient than the advanced water purification facilities in terms of facilities investment cost, and if the coagulation filteration was used for T-P removal, the activated carbon process among the advanced water purification techniques was more efficient in terms of facilities investment cost. In this study, the effects of the T-P removal process that will be additionally introduced according to the tightening of the effluent T-P standard were analyzed within a limit. The actual benefits of improved T-P concentration in the water source will provide diverse values, including the leisure water, environment improvement water, eco-system in the water source and industrial water, in addition to the advanced water purification.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1996년도 추계학술대회 논문집
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• pp.313-317
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• 1996

This work deals with description of gas purifing system to product high pure helium gas using low temperature absorption. The system controls temperature of heaters, open/close of solenoid valves and levels of liquid nitrogen to purify a raw gas and continuously products purified gas with perfoming alternatively purification and regeneration. We develop the monitoring and control program to monitor the gas purification process on real-time and control the process time with checking the impurities in purified gas. From the result of system operation, the developed monitoring and control system continuously products high pure helium gas with reducing impurities in raw gas to permitted limits(less than 0.01 ~ 0.05 ppm)

• Journal of the Korean Wood Science and Technology
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• 제30권3호
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• pp.12-17
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• 2002

The extracellular invertase (EC 3.2.1.26) (Candida utilis) preparation was obtained from the liquid medium after desalting and freeze drying. This prepared enzyme was used for the comparative purification on 4 activated matrices by liquid column affinity chromatography method. In this method there were used controlled porous glass (CPG) silanized covalently activated by keratin, silanized silica gel and silica gel covalently covered by keratin. It was found that the invertase purification process was better using both CPG matrices (silanized CPG and keratin activated CPG) than these with two silica gel supports. Also the elution coefficient of the invertase from the two CPG columns was about 93 to 94%. Two silica gel supports found to be superior in terms of purification efficiency. The invertase purification process was confirmed by PAGE electrophoresis.

• 한국재료학회지
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• 제26권6호
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• pp.306-310
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• 2016

To produce 4N grade high-purity silica powder from natural ore, the mineralogical characteristics of natural silica ore were investigated and their effects on the purification process were revealed. The Chinese silica mineral ore used was composed of iron and aluminum as main impurities and calcium, magnesium, potassium, sodium, and titanium as trace impurities; these trace impurities generally exist as either single oxides or complex oxides. It was confirmed that liberation and acidic washing of the impurities were highly dependent on the particle size of the ground silica ore and on its mineralogical characteristics such as the distribution and phases of existing impurities. It is suggested that appropriate size reduction of silica ore should be realized for optimized purification according to the origin of the natural silica ore. A single step purification process, the mechano-chemical washing (MCW) process, was proposed and verified in comparison with the conventional multi step washing process.

• Journal of Microbiology and Biotechnology
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• 제19권4호
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• pp.416-423
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• 2009

Hepatitis B core antigen(HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus(HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by anion exchange expanded bed adsorption chromatography integrated with a cell disruption process. This online capture process has shortened the process time and eliminated the "hold-up" period that may be detrimental to the quality of target protein. The eluted product from the expanded bed adsorption chromatography was subsequently purified using size-exclusion chromatography. The results showed that this novel purification protocol achieved a recovery yield of 45.1% with a product purity of 88.2%, which corresponds to a purification factor of 4.5. The recovered HBcAg is still biologically active as shown by ELISA test.

• 환경위생공학
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• 제23권2호
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• pp.55-63
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• 2008

All over the world, the existing process of water purification needs more flocculants and chlorine due to a gradual decline in the quality of source water. Therefore, the problem of the remaining aluminium and DBPs in purified water is on the rise. To solve this problem, the process of membrane filter has recently come into the spotight. This study reaches the following conclusions concerning TMP variation in order to solve the dropping of flux throgh a membrane filter when operating a membrane filter system in the process of water purification. 1. In case that a cohesion-precipitation process was introduced to pre-treatment of a membrane filter, initial TMP was very satisfactory(0.27kg/cm) in producing the constantly safe quality of water, $0.04{\sim}0.1$(mean 0.05) NTU by pouring 2mg/l of PACI(10% $Al_2O_3$) used for the existing process of water purification in high-density turbidity at a dry or flood season and at occurrence of high algae. 2. As flux increased at 0.5m/day.m, TMP increased 0.05 kgf/cm. 3. As filtering, operation mode of PVDF MF membrane filtering was 48 minutes and 1 cycle of back washing was 42 minutes, flux was increased 1.5m/day.m and TMP increased $0.25{\sim}0.27kgf/cm$. Without back washing, TMP increased 0.03 kgf/cm per a cycle.