• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1249-1255
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• 2004

This study has developed Corynebacterium ammoniagenes (c. ammoniagenes) purine gene transcriptional reporters (purF-lacZ and purE-lacZ) that function in Escherichia coli (E. coli) DH5a. After transformation of a C. ammoniagenes gDNA library into E. coli cells harboring either purF-lacZ or purE-lacZ, C. ammoniagenes clones were obtained that repress purF-lacZ and purE-lacZ gene expression. The potential purE and purF regulatory genes are homologous to the genes encoding transcription regulators, the regulatory subunit of RNA polymerase, and genes for purine nucleotide biosynthesis of various bacteria. The C. ammoniagenes purE-lacZ and purF-lacZ reporters were repressed by adenine and guanine within E. coli, indicating similarity in the regulatory mechanism of purine biosynthesis in C. ammoniagenes and E. coli. Gene regulation of pur-lacZ by adenine and guanine was partly abolished in cells expressing potential purine regulatory genes, indicating functionality of the purine gene regulators in repression of purE-lacZ and purF-lacZ. The purE-lacZ and purF-lacZ reporters can be used for the screening of genes involved in the regulation of the de novo synthesis of the purine nucleotides.

• Korean Journal of Microbiology
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• v.45 no.3
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• pp.233-238
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• 2009

The expression of Corynebacterium ammoniagenes purF was analyzed by utilizing a plasmid carrying a cat gene fused to the purF promoter region. Adenine and guanine repressed the expression of the purF gene by 20~30% but hypoxanthine did not exert such repressive effect. The expression purF was maximal at the late log phase and remained constant throughout the stationary phase. Promoter $P_{180}$ which was developed in C. glutamicum was also functional in C. ammoniagenes, achieving maximal activity at the late log phase. The promoter outperformed Escherichia coli $P_{tac}$ promoter by 40~50% level. DNA-affinity purification identified a protein which could bind to the promoter region of the purF gene. The protein showed high similarity to the CRP-family transcriptional regulator encoded by NCgl0120 in C. glutamicum. The size of the screened protein agreed with the expected protein size from the ORF NCgl0120. The corresponding gene in C. ammoniagenes encoded a 42 kDa polypeptide composed of 400 amino acids with expected pI of 4.9. The encoded protein showed 14.1% and 15.8% identity with E. coli and Bacillus subtilis PurR, respectively, suggesting that the isolated protein might be a novel type of regulatory protein involved in the regulation of purine metabolism.

• Korean Chemical Engineering Research
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• v.46 no.6
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• pp.1039-1042
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• 2008

In order to increase a functionality, OH value, for a recycled polyol prepared from the glycolysis reaction of a waste polyurethane rigid foam(PUR), the effect of an addition of pentaerythritol(PEN, functionality(f)=4) or sorbitol(SOR, f=6) to the its glycolysis reactor on the prepared polyol functionality and the mechanical properties of the polyurethane prepared using it was investigated. The OH values increased from 2.2 for a virgin to 2.8 for the recycled polyol. There was an increase in the mechanical properties including dimensional stability for PUR prepared using the recycled polyol, in which the increased OHs provided higher crosslinking density during PUR synthesis. In addition, the amount of the recycled polyol in the polyol system increased to from 8 to 20 wt% to give better mechanical properties to the PUR.

• Journal of the Korean Wood Science and Technology
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• v.50 no.3
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• pp.167-178
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• 2022

To investigate the influence of manufacturing environment on bonding performance of mixed cross laminated wood (CLT) using polyurethane (PUR) adhesive, a boiling water soak delamination test according to the temperature and relative humidity was conducted. The 5-ply mixed CLT consisted of Japanese Larch for external and middle layer and yellow poplar for internal layer. The PUR adhesives with different opening times of 10 and 30 minutes were used. The mixed CLT was manufactured according to pressing times of PUR and manufacturing environments of summer and winter. In case of summer environment, the delamination rate of the mixed CLT with pressing time of 4 hours using a PUR adhesive with open time of 10 minutes met the requirements of KS F 2081. In case of winter environment, the delamination rate of the mixed CLT didn't meet the requirements of KS standard. However, it was possible to confirm the effect of improving the adhesive performance by adjusting the pressing time according to the open time of the adhesive under the manufacturing conditions. The delamination rate of CLT with open time 30 minutes PUR, manufactured by indirect moisture supply methods was 11.2% better than direct moisture supply methods. As a result of delamination test in the same condition of relative humidity and adhesive, it was found that the temperature of manufacturing environment influences the adhesive performance.

• Journal of the Korean Wood Science and Technology
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• v.52 no.1
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• pp.58-69
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• 2024

To evaluate the adhesive characteristics of mixed cross-laminated timber (CLT) using domestic softwoods structural lumber proposed by KS F 3020 and yellow poplar, penetration depth of adhesive and thickness of bonding line were analyzed based on the results of boiling water soaking delamination. 3 Types of adhesives and 2 types of major layer were divided into a 5 ply CLT using yellow popular as minor layer. The bonding performance of the mixed CLT as structural members was evaluated based on the KS F 2081. The thickness of bonding line between layers of the mixed CLT was measured with a scanning electron microscope, and the adhesive penetration depth in the layer members was measured with an optical microscope. As a result of boiling water soaking delamination test of mixed CLT, the CLT specimens using PRF and PUR adhesives met the requirements of KS F 2081. It was verified that the penetration path of the adhesive in the layes was mainly through the tracheid cell in the case of Japanese larch and Korean red pine layers, and through the vessel and radial tissue in yellow popular layers. The penetration depth of the adhesive was the highest for the PRF adhesive under the same pressing time conditions, and the thickness of the bonding line was in inverse proportion to the penetration depth in the case of the PUR adhesive.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2010.04a
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• pp.289-296
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• 2010

This study is aiming at analyzing correlation between flashover's time and maximum smoke production rate's time on sandwich panel specimens. For this analysis, KS F ISO 137481-1 has been performed for 23 kinds of sandwich panels which mainly consist of EPS, PIR, PUR, Glass wool and so on. KS F ISO 13784-1 represents real scale reaction-to-fire test and focuses on measuring flashover phenomenon rather than other tests as like ISO 5660-1 and SBI.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2010.10a
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• pp.167-170
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• 2010

This study is aiming at analyzing correlation between flashover's time and maximum smoke production rate's time on sandwich panel specimens. For this analysis, KS F ISO 137481-1 has been performed for 23 kinds of sandwich panels which mainly consist of EPS, PIR, PUR, Glass wool and so on. KS F ISO 13784-1 represents real scale reaction-to-fire test and focuses on measuring flashover phenomenon rather than other tests as like ISO 5660-1 and SBI.

• Journal of the Korean Society of Costume
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• v.33
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• pp.27-39
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• 1997

The purpose of this study was to classify the attributes of brand image criteria of women's shoes to compose the perceptual map of the brand by factor analysis and to examine the differences in brand preferences and purchase methods of shoes according to demographic variables. 10 brand names were selected for the study Samples were 271 women in Seoul Korea :143 were college students and 128 were career women.The data were analyzed using factor analy-sis multiple regression analysis one-way ANOVA Duncan's multiple range test x2-test t-test. The results of the study were the -followings: 1. Four segments of brand image attributes of women's shoes derived by factor analysis: F. 1. 'utility' F.2'appearance' ; F. 3 'sales promotion' ; F.4 'financial factor'. 2. As the result of draw up the perceptual map 'landrover' was high in utility but low in appearance 'Misope' and 'Mook' was low in utility but high in appearance. 'Fashion Leader' was in the nearest ideal direction to the utility and appearance. 3. The preference level of the shoes brand name was in order of the 'Fashion Leader'. 'Mook' and 'Soda' But consumers possessed 'Landrover' the most 4. There were significant differences among preference level of ' Landrover' and 'Misope' according to the social class. There were sig-nificant differences among possession level of 'Misope' and 'Soda' according to the social class 5. the middle and lower class consumers used an exchange ticket during the bargain sales more than upper class when they pur-chase shoes.

• Molecules and Cells
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• v.20 no.2
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• pp.297-302
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• 2005

The integrity and proper functioning of telomeres require association of telomeric DNA sequences with specific binding proteins. We have characterized PLP-1, a $PUR{\alpha}$ homolog encoded by F45E4.2, which we previously identified as a candidate double stranded telomere binding protein, by affinity chromatography followed by mass spectrometry. PLP-1 bound double-stranded telomeric DNA in vitro as shown by competition assays. Core binding was provided by the third and fourth nucleotides of the TTAGGC telomeric repeat. This is quite different from the binding sequence of CEH-37, another C. elegans telomere binding protein, suggesting that multiple proteins may bind nematode telomeric DNA simultaneously in vivo.

• Journal of the Korean Society of Costume
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• v.35
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• pp.181-193
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• 1997

The objectives of this study were to classify bedclothes purchase motives and to examine how bedclothes purchase mo-tives and design preferences very accord-ing to age and income. Samples were 217 housewives residing in metropolitan area. Questionnaire included 17 Likert type items of bedclothes pur-chase motive measure 12 items of color preference 7 items of pattern (floral geo-metric abstract stripe plaid polka dot, and paisley pattern). The results of the study were as fol-lows. 1. 5 factors of bedclothes purchase mo-tives were derived by factor analysis ; F .1 'design': F 2. "brand' ; F.3. 'deficiency'; F. 4. 'fabric' F.5. 'economical reason' 2. Subjects perceived design and utility area to be important motives for bed-clothes purchase. 3. Cholor preference of bedclothes was in the order of white pale blue pale green and pale orange. Pattern preference was in the order of stripe plaid solid color floral and polka dot pattern. The combi-nation of patterned fabric and solid color fabric was liked better than the combina-tion of analogic color and the combination of contrasting color. 4. The women in their 20's liked navy blue red stripe plaid pattern and solied color better than 30's and 40's . 40's liked abstract and paisley pattern better then 20's and 30's 5. Low income group lied navy blue and solid color fabric more than the mid-dle and high income group and liked ab-stract pattern less than the middle and high income group. 6. Women in their 20's perceived design to be important motive more than 30's and 40's. 30's perceived brand to be im-portant motive than 20's and 40's 7. High income group perceived design to be important motive more than the middle and low income group. Low in-come group perceived brand and fabric to be less important motives than the middle and high income group, The present findings provide that age and income had a significant effect on bedclothes purchase motives and design preferences of houesewives. The results that white color and the combination of patterned fabric and solid color fabric were liked best indicated that women prefered clean image and chacteristics de-sign of bdeclothes.