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PLP-1 Binds Nematode Double-stranded Telomeric DNA  

Im, Seol Hee (Molecular Aging Research Center, Yonsei University)
Lee, Junho (Molecular Aging Research Center, Yonsei University)
Publication Information
Molecules and Cells / v.20, no.2, 2005 , pp. 297-302 More about this Journal
Abstract
The integrity and proper functioning of telomeres require association of telomeric DNA sequences with specific binding proteins. We have characterized PLP-1, a $PUR{\alpha}$ homolog encoded by F45E4.2, which we previously identified as a candidate double stranded telomere binding protein, by affinity chromatography followed by mass spectrometry. PLP-1 bound double-stranded telomeric DNA in vitro as shown by competition assays. Core binding was provided by the third and fourth nucleotides of the TTAGGC telomeric repeat. This is quite different from the binding sequence of CEH-37, another C. elegans telomere binding protein, suggesting that multiple proteins may bind nematode telomeric DNA simultaneously in vivo.
Keywords
Binding Protein; C. elegans; Core Sequence; Gel Shift Assay; PLP-1; Telomere;
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