• Title/Summary/Keyword: pulse time

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Production of Cloned Embryos by Nuclei Transfer and Electronic Cell Fusion from Bovine Fetal Fibroblasts (Bovine Fetal Fibroblasts를 이용한 핵이식 및 세포융합에 관한 연구)

  • 이병천;박종임;조종기;김기연;신수정;용환율;황우석
    • Journal of Embryo Transfer
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    • v.14 no.2
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    • pp.107-111
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    • 1999
  • The present study was performed to evaluate the best electric fusion condition in nuclear transfer, Korean Native Cattle fibroblasts were used as nucleic donors. Oocytes from slaughterhouse were matured in vitro for 22 h and enucleated. Each individual cells were transferred into enucleated ocytes and reconstructed embryo were placed into the fusion chamber. In experiment 1, pulse were performed by altering pulse duration at 1. 75kv/cm, 1 time. When pulse duration is 30$mutextrm{s}$, fusion and development rates is higher than other conditions. In experiment 2, the effect of different pulse number were studied at the pulse duration 30$mutextrm{s}$ and the same pulse intensity. When pulse number was one, fusion rates were higher than other conditions. The fused embryos were moved to culture medium and assessed their development to blastocyst. These results showed that best fusion condition was 30$mutextrm{s}$ and one time. And the fibroblasts derived from Han Woo can be reprogrammed by nuclear transplantation and develop subsequently in vitro.

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Time Domain Analysis of Dispersion Characteristics of Pulse for MMIC Design (초고주파 집적회로 설계를 위한 펄스의 시간영역 분산 특성 해석)

  • Kim, Gi-Rae
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.8
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    • pp.1755-1760
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    • 2013
  • In this paper, when the pulses propagate on a uniform microstrip line, the distortion of pulse signal caused by dispersion is investigated in time domain. We analyzed dispersion characteristics according to dielectric constant and structure of transmission line, and compared propagating characteristics for square and gaussian pulse according to pulse width, pulse amplitude, and propagation velocity. The results of this paper are compatible to the trade-off determination of relative permittivity, substrate height, strip width and pulse width of signal pulse when a design of MIC and MMIC is necessary.

A Study on the Pulse Peak Voltage and Cascading Ratio of Compact Pulse Generator using Cascading Method (Cascading 방식을 적용한 펄스발생기의 펄스전압 변성 및 Cascading 비율 특성)

  • Joung, Jong-Han;Kim, Hee-Je
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.50 no.7
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    • pp.329-333
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    • 2001
  • The pulsed poser system has been widely used to many applications, such as E/P(Electrostatic Precipitator), DeNox/DeSOx power system, ozon generator, etc. A pulse energy efficiency for load depends on the rising time, peak value, pulse duration and impedance matching, etc. The pulse generator generally required for short pusle duration and high peak value was forced to consider its volume and economy. In this study, developing a compact pulse generator that applied for cascading method to be made of two pulse transformers, we compared cascading voltage with non cascading one by applying the pulse energy to load. Adopting cascading technique to pulse transformer, we found that average cascading voltage was about 60[%] of theoretical value. Maximum cascading ratio was calculated at 60 times compared with non cascading voltage.

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Pulse Shape Design for Ultra-Wideband Radios Using Projections onto Convex Sets (POCS를 이용한 초광대역 무선통신의 펄스파형 설계)

  • Lee, Seo-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.3A
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    • pp.311-318
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    • 2008
  • We propose new pulse shapes for FCC-compliant ultra-wideband (UWB) radios. The projections onto convex sets (POCS) technique is used to optimize temporal and spectral shapes of UWB pulses under the constraints of all of the desired UWB signal properties: efficient spectral utilization under the FCC spectral mask, time-limitedness, and good autocorrelation. Simulation results show that for all values of the pulse duration, the new pulse shapes not only meet the FCC spectral mask most efficiently, but also have nearly the same autocorrelation functions. It is also observed that our truncated (i.e., strictly time-limited) pulse shapes outperform the truncated Gaussian monocycle in the BER performance of binary TH-PPM systems for the same pulse durations. The POCS technique provides an effective method for designing UWB pulse shapes in terms of its inherent design flexibility and joint optimization capability.

Error Analysis of Reaction Wheel Speed Detection Methods (반작용휠 속도측정방법의 오차 분석)

  • Oh, Shi-Hwan;Lee, Hye-Jin;Lee, Seon-Ho;Yong, Ki-Lyuk
    • Journal of Astronomy and Space Sciences
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    • v.25 no.4
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    • pp.481-490
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    • 2008
  • Reaction wheel is one of the actuators for spacecraft attitude control, which generates torque by changing an inertial rotor speed inside of the wheel. In order to generate required torque accurately and estimate an accurate angular momentum, wheel speed should be measured as close to the actual speed as possible. In this study, two conventional speed detection methods for high speed motor with digital tacho pulse (Elapsed-time method and Pulse-count method) and their resolutions are analyzed. For satellite attitude maneuvering and control, reaction wheel shall be operated in bi directional and low speed operation is sometimes needed for emergency case. Thus the bias error at low speed with constant acceleration (or deceleration) is also analysed. As a result, the speed detection error of elapsed-time method is largely influenced upon the high-speed clock frequency at high speed and largely effected on the number of tacho pulses used in elapsed time calculation at low speed, respectively.

Numerical Analysis on Heat Transfer Characteristics in Silicon Boated by Picosecond-to-Femtosecond Ultra-Short Pulse Laser (펨토초급 극초단 펄스레이저에 의해 가열된 실리콘 내의 열전달 특성에 관한 수치해석)

  • 이성혁;이준식;박승호;최영기
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.10
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    • pp.1427-1435
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    • 2002
  • The main aim of the present article is numerically to investigate the micro-scale heat transfer phenomena in a silicon microstructure irradiated by picosecond-to-femtosecond ultra-short laser pulses. Carrier-lattice non-equilibrium phenomena are simulated with a self-consistent numerical model based on Boltzmann transport theory to obtain the spatial and temporal evolutions of the lattice temperature, the carrier number density and its temperature. Especially, an equilibration time, after which carrier and lattice are in equilibrium, is newly introduced to quantify the time duration of non-equilibrium state. Significant increase in carrier temperature is observed for a few picosecond pulse laser, while the lattice temperature rise is relatively small with decreasing laser pulse width. It is also found that the laser fluence significantly affects the N 3 decaying rate of Auger recombination, the carrier temperature exhibits two peaks as a function of time due to Auger heating as well as direct laser heating of the carriers, and finally both laser fluence and pulse width play an important role in controlling the duration time of non-equilibrium between carrier and lattice.

Implementation of High Accurate Level Sensor System using Pulse Wave Type Magnetostriction Sensor (펄스파 자왜 센서를 이용한 고정밀 액위 센서 시스템의 실현에 관한 연구)

  • Choi, Woo-Jin;Lee, John-Tark
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.62 no.3
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    • pp.395-400
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    • 2013
  • In this paper, we introduce the implementation of high accurate level sensor system using the pulse wave type magnetostriction sensor. When a current pulse flows along the waveguide, the magnetic field also propagates towards the end of waveguide. When this magnetic field just passes the position of the magnet for level detection, the resultant magnetic field by these two magnetic fields makes a torsional reflected signal. This is used to calculate the time difference between a interrogation pulse wave and this torsional reflected signal. The key elements and characteristics were investigated to implement level sensor system based on this principle. We introduce a method to calculate the speed of ultrasonic reflected signal and how to make a model of sensing coil. In particular, we experiment with the characteristics of the torsional reflected signal according to the changes of the interrogation voltage and displacement. To make high accurate level sensor system, two methods were compared. One is to use the comparator and time counter, the other is STFT(Short Time FFT) which is capable of the time-frequency analysis.

T2 Relaxographic Mapping using 8-echo CPMG MRI Pulse Sequence

  • E-K. Jeong;Lee, S-H.;J-S. Suh;Y-Y wak;S-A. Shin;Y-K. Kwon;Y. Huh
    • Journal of the Korean Magnetic Resonance Society
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    • v.1 no.1
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    • pp.7-20
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    • 1997
  • The mapping of the spin-spin relaxation time T2 in pixed-by-pixel was suggested as a quantitative diagnostic tool in medicine. Although the CPMG pulse sequence has been known to be the best pulse sequence for T2 measurement in physics NMR, the supplied pulse sequence by the manufacture of MRI system was able to obtain the maximum of 4 CPMG images. Eight or more images with different echo time TEs are required to construct a reliable T2 map, so that two or more acquisitions were required, which easily took more than 10 minutes. 4-echo CPMG imaging pulse sequence was modified to generate the maximum of 8 MR images with evenly spaced echo time TEs. In human MR imaging, since patients tend to move at least several pixels between the different acquisitions, 8-echo CPMG imaging sequence reduces the acquisition time and may remove any misregistration of each pixel's signal for the fitting T2. The resultant T2 maps using the theoretically simulated images and using the MR images of the human brain suggested that 8 echo CPMG sequence with short echo spacing such as 17∼20 msec can give the reliable T2 map.

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Development of a real-time gamma camera for high radiation fields

  • Minju Lee;Yoonhee Jung;Sang-Han Lee
    • Nuclear Engineering and Technology
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    • v.56 no.1
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    • pp.56-63
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    • 2024
  • In high radiation fields, gamma cameras suffer from pulse pile-up, resulting in poor energy resolution, count losses, and image distortion. To overcome this problem, various methods have been introduced to reduce the size of the aperture or pixel, reject the pile-up events, and correct the pile-up events, but these technologies have limitations in terms of mechanical design and real-time processing. The purpose of this study is to develop a real-time gamma camera to evaluate the radioactive contamination in high radiation fields. The gamma camera is composed of a pinhole collimator, NaI(Tl) scintillator, position sensitive photomultiplier (PSPMT), signal processing board, and data acquisition (DAQ). The pulse pile-up is corrected in real-time with a field programmable gate array (FPGA) using the start time correction (STC) method. The STC method corrects the amplitude of the pile-up event by correcting the time at the start point of the pile-up event. The performance of the gamma camera was evaluated using a high dose rate 137Cs source. For pulse pile-up ratios (PPRs) of 0.45 and 0.30, the energy resolution improved by 61.5 and 20.3%, respectively. In addition, the image artifacts in the 137Cs radioisotope image due to pile-up were reduced.

A Study on Malfunction Mode and Failure Rate Properties of Semiconductor by Impact of Pulse Repetition Rate (펄스 반복률에 의한 반도체 소자의 오동작 모드와 고장률에 관한 연구)

  • Park, Ki-Hoon;Bang, Jeong-Ju;Kim, Ruck-Woan;Huh, Chang-Su
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.28 no.6
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    • pp.360-364
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    • 2015
  • Electronic systems based on solid state devices have changed to be more complicated and miniaturized as the electronic systems developed. If the electronic systems are exposed to HPEM (high power electromagnetics), the systems will be destroyed by the coupling effects of electromagnetic waves. Because the HPEM has fast rise time and high voltage of the pulse, the semiconductors are vulnerable to external stress factor such as the coupled electromagnetic pulse. Therefore, we will discuss about malfunction behavior and DFR (destruction failure rate) of the semiconductor caused by amplitude and repetition rate of the pulse. For this experiment, the pulses were injected into the pins of general purpose IC due to the fact that pulse injection test enables the phenomenon after the HPEM is coupled to power cables. These pulses were produced by pulse generator and their characteristics are 2.1 [ns] of pulse width, 1.1 [ns] of pulse rise time and 30, 60, 120 [Hz] of pulse repetition rate. The injected pulses have changed frequency, period and duty ratio of output generated by Timer IC. Also, as the pulse repetition rate increases the breakdown threshold point of the timer IC was reduced.