• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.205-211
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• 2012

Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.

• Journal of Haehwa Medicine
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• v.13 no.2
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• pp.179-186
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• 2004

Now many sustitution and false articles is used in korea instead of gegen. To use gegen correctly, we will make a quilitative certificational plan of gegen to investigate all of lieraturea, records and documents. And we could reach conclusions as folloews. 1) Source As a source of gegen, we think all of Pueraria lobata Ohwi and Pueraria thomsonii Benth can be used in korea and china. 2) Harvesting time. According to a data, content of effective components is high when harvested in January and February. Though there is no problem because domestic harvesting and working time is between January and February, we think it need to sure well-founded datas. 3) Standars of goods According to cutting methods, gegen divide into 'jiaogegen' and 'piangegen' from users So there is no problem at cutting methods because it is not a affective factor. 4) Processing A processing method is a best important case in express quility of herb. According to a data, effective components of peeled gegen is much less than that of non-peeled geeen. A tested samples is non-peeled gegen at a researh, they received suitable judgments in all of items. Especially we got a result content of puerarin is much more than 2.0%. But they were not washed in water, it is a problem that there is bleaching effect by almost drying at a briquat. So a processing method is to wash in water with unavoidable turnning to brown, to prevent this grgrn must be dried in bulk at low temporature. And we think that content of puerarin must be looked upward.

• Korean Journal of Oriental Medicine
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• v.11 no.2
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• pp.167-178
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• 2005

As a part of the quality control of herbal prescriptions which has been used for diabetes and related diseases, a reversed-phase liquid chromatographic method was developed for the simultaneous quantification of the three marker compounds, puerarin (Puerariae Radix), glycyrrhizin (Glycyrrhizae Radix), schizandrin (Schizandrae Fructus) in Oc Chun San. The HPLC analysis method was validated for parameters such as linearity, Limits of Detection(LOD), quantification(LOQ), repeatability, stability and recovery.

• YAKHAK HOEJI
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• v.40 no.2
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• pp.177-182
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• 1996

The contents of aloesin in aloe preparations were determined by HPLC. Aloesin was extracted 3 times with ethanol for 30 minutes. The ethanol extract was concentrated and suspend ed in saturated NaCl aqueous solution and successively partitioned with dichloromethane, n-butanol. Prepared samples were analyzed by HPLC on a reverse column(Inertsil ODS-2). In assay, internal standard was a puerarin and regression of calibration curve was 0.998. Recoveries of aloesin added to aloe preparation were 98~123(%).

• Korean Journal of Human Ecology
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• v.9 no.2
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• pp.215-221
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• 2000

Pueraria Radix has known to contain several ingredients of medical action. However, the bitter taste of Pueraria Radix has been an obstacle to develope the products and improve the added value of Pueraria Radix. To moderate the bitter taste of extracts from Pueraria Radix, charcoal powder was used successfully as an adsorbent. The component of the bitter taste from Pueraria Radix was hydrophobic, which mostly eluted with 40-60% ethanol and estimated to be acidic. Puerarin, the essential medical ingredient remained after the adsorption and seemed not be affected by the adsorption to charcoal powder.

• Natural Product Sciences
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• v.10 no.4
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• pp.152-167
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• 2004

Traditional Chinese Medicines (TCM) have attracted great interest in recent researchers as alternative medicines for incurable diseases. This review focuses on qualitative and quantitative analytical approaches for bioactive metabolites of components flavonoids and saponins of traditional Chinese medicines by TLC system, although various methods have been introduced. Emphasis will be put on the processes of metabolite extraction from intestinal bacterial cultures or urines, separation (mobile phase) and detection. The identified metabolites by selection of extraction solvent and detection methods are also discussed. In addition, metabolite determinations of flavonoids (baicalin, apiin, rutin, quercetin, quercitrin, kaempferol, diosmin, hesperidin, poncirin, naringin, puerarin, daidzin, daidzein, tectoridin) and saponins (ginsenosides, kalopanaxsaponins, glycyrrhizin, chiisanoside, saikosaponins, soyasaponins) in culture fluid, in urine and in some herbal formula extracts are summarized. These bioactive metabolites of these components by intestinal microflora should be connected to pharmacological actions.

• Korean Journal of Pharmacognosy
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• v.28 no.1
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• pp.42-47
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• 1997

The prescription of Gal Gun Tang, which has been used for treatment of cold, fever, and muscular pain in Chinese herb medicine, is produced in the form of decoction However, the storage problem for this dosage form remains unsettled. Using HPLC and GC, we examined quantitative change of major constituents caused by time-progress and temperature-change. Nine major constituents, such as cinnamaldehyde, cinnamic acid, ephedrine, puerarin, paeoniflorin, daidzin, benzoic acid, glycyrrhizin and liquiritin, were selected as references. The content of cinnamaldehyde significantle decreased by the increase of temperature. While that of cinnamic acid increased. Benzoic acid showed the most significant change of the content in three months at $40^{\circ}C$. It is suggested that most of constituents are considerably stable when kept frozen.

• Applied Chemistry for Engineering
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• v.29 no.5
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• pp.503-509
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• 2018

In this study, we used the ultrasonic extraction process as a method to extract antioxidant substances from kudzu, and measured the content of iso-flavonoids puerarin, daidzein, daidzin contained in kudzu. The response surface methodology which is a statistical analysis method for optimizing the extraction amount of iso-flavonoids from the kudzu and the process condition for maximizing the yield was applied. It is the final objective of this study to effectively derive the condition of the process that matches the target response with a minimum number of experiments and analyze the effect of each process condition on the response. In the response surface methodology, the central composite design was applied and the optimum condition was analyzed, and the three independent variables were set to ultrasonic irradiation time, volume ratio of ethanol/ultrapure water, ultrasonic irradiation power. Using the response surface methodology, the optimum conditions with the maximum extraction yield and the content of iso-flavonoids were evaluated as ultrasonic irradiation time (24.75 min), ethanol / ultrapure water volume ratio (39.75 vol%), ultrasonic irradiation power (592.36 W). The overall satisfaction level appears as high as 0.8938, which is recognized at a significance level within 5%. As a result of analyzing the optimization process, it was confirmed that the ultrasonic irradiation time is the factor that most affects the responses.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.217-223
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• 2013

Pueraria lobata, a medicinally important leguminous plant produces various isoflavones including puerarin, daidzin and daidzein which are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in human intestines. In this study, we described callus proliferation and isoflavone production in callus of Korean wild arrowroot and dihydrodaidzein biosynthesis in callus extract fermented with Pediococcus pentosaceus. Proliferation was the best at callus cultured in the medium containing 1.0 mg/L TDZ and 1.0 mg/L NAA at light condition for 12 days. Puerarin was significantly more produced at callus cultured in the medium containing 2.0 mg/L kinetin and 1.0 mg/L NAA at dark condition for 16 days, but daidzin and daidzein were not significant. Callus extract was successfully fermented with P. pentosaceus and dihydrodaidzein, which is one of equol precursors formed by biotransformation, was confirmed to be produced. These results will facilitate mass production of callus and isoflavones as equol precursors from Korean wild arrowroot and can be applied for the production of equol by biotransformation in vitro.

• The Journal of Korean Medicine
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• v.31 no.6
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• pp.8-15
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• 2010

Objectives: We investigated to develop and validate HPLC-PDA methods for simultaneous determination of eight constituents in Galgeun-tang (GGT). Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 230 nm, 254 nm, and 280 nm, were used for quantification of the eight marker components of GGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results: Calibration curves were acquired with $r^2$ > 0.9999, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each component was in the range of 87.33-101.38%, with an RSD less than 7.0%. The contents of the eight components in GGT were 1.98-12.17 mg/g. Conclusions: The established method will be applied for the quantification of marker components in GGT.