• Journal of Microbiology
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• 제35권1호
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• pp.53-60
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• 1997

Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 isolated from the polluted environment are capable of degrading biphenyl and 4-chlorobiphenyl (4CB) to produce benzoic acid and 4-chlorobenzoic acid (4CBA) respectively, by pcbABCD-encoded enzymes. 4CBA can be further degraded by Pseudomonas sp. DJ-12, but not by Pseudomonas sp P20. However, the meta-cleavage activities of 2, 3-dihydroxybiphenyl (2, 3-DHBP) and 4-chloro-2, 3-DHBP dioxygenases (2, 3-DHBD) encoded by pcbC in Pseudomonas sp. P20 were stronger than Pseudomonas sp. DJ-12. In this study, the pcbC gene encoding 2, 3-DHBD was cloned from the genomic DNA of Pseudomonas sp. P20 by using pKT230. A hybrid plasmid pKK1 was constructed and E. coli KK1 transformant was selected by transforming the pKK1 hybrid plasmid carrying pcbC into E. coli XL1-Blue. By transferring the pKK1 plasmide of E. coli KK1 into Pseudomonas sp. DJ-12 by conjugation, a recombinant strain Pseudomonas sp. P20, Pseudomonas sp. DJ-12, and the recombinant cell assay methods. Pseudomonas sp. DJ12-C readily degraded 4CB and 2, 3-DHBP to produce 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA), and the resulting 4CBA and benzoic acid were continuously catabolized. Pseudomonas sp. DJ12-C degraded 1 mM 4CB completely after incubation for 20 h, but Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 showed only 90% and Pseudomonas sp. DJ-12 had, but its degradation activity to 2, 3-DHBP, 3-methylcatechol, and catechol was improved.

• 식물병연구
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• 제9권3호
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• pp.174-178
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• 2003

콩 탄저병의 생물학적 방제를 위하여 병원균 Colletotrichum truncatum과 C. gloeosporioieds에 대한 길항세균 Pseudomonas sp.를 분리 동정하였다. Pseudomonas sp.의 균사생장 억제효과는 탄저병 살균제 benomyl, fluazinam과 비슷하였으며, 콩에 대한 병원성은 거의 없었다. Pseudomonas sp. 의 종자처리는 C. truncatum 을 접종한 콩 종자의 출현율을 현저히 증가시켰고, 경엽처리한 경우 잔효성은 낮았지만 처리 2주 후의 방제효과는 benomyl, fluazinam과 비슷하게 높았다.

• 한국안광학회지
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• 제9권2호
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• pp.353-359
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• 2004

Pseudomonas aeruginosa에 대한 soft contact lens 다목적 용액(multi purpose solution, MPS)의 항균효과의 검정을 탁도측정법에 의해 실시하였다. Muller Hinton Broth 액체배지에 pseudomonas aeruginosa를 배양한 후 5개사의 5종류의 MPS를 이용해 각각 시간별로 처리해 대조군과 비교하여 항균효과를 비교해 본 결과 한 제품을 제외하고는 거의 비슷한 효과를 나타내었다. 또한 동일한 조건하에서 MPS의 항균효과는 구성성분, pH에 따라 달라질 수 있으며 5개 회사제품 중 4개에서 pseudomonas aeruginosa에 대한 항균효과가 있는 것으로 나타났다.

• 한국환경과학회지
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• 제14권10호
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• pp.955-963
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• 2005

From sludge of S municipal wastewater treatment plant in Busan, Korea, we isolated the denitrifier DN-9 which showed the ability of denitrification under aerobic conditionby the color change and gas formation in liquid culture with Giltay medium. The isolated strain was identified as Pseudomonas sp. DN-9 on the basis of the morphological, physiological, biochemical and nucleotide sequence analysis of l6S rRNA. The isolated strain, Pseudomonas sp. DN-9, has cytochrome $cd_1$, nirS of nitrite reductase. By the co-existance of additional ammonium and nitrate ion, the strain was not affected largely on growth in SL series broth. It seemed the result of denitrification. Although Pseudomonas sp. DN-9 has a good nitrate reduction activity under aerobic condition, the activity is less than Pseudomonas stutzeri in same cultivation condition. However, Escherichia coli had little the activity of aerobic denitrification and Pseudomonas putida showed lower activity of aerobic denitrification than Pseudomonas sp. DN-9 and Pseudomonas stutzeri in this study.

• 대한의생명과학회지
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• 제25권4호
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• pp.321-330
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• 2019

Carbapenem is recently considered as the last resort of the therapeutics for gram negative bacterial infection. Increasing of organisms producing metallo-β-lactamase (MBL), we have difficulty in choosing the antimicrobial agents. Among 345 clinical isolates of Pseudomonas spp., 61 isolates (17.7%) were positive for the modified imipenem or meropenem-Hodge test and 55 isolates (15.9%) were positive for the imipenem-EDTA + SMA double disk synergy test (DDS). PCR and sequencing of bla_VIM-2-allele and bla_IMP-1-allele showed that 17 isolates of Pseudomonas aeruginosa, 9 isolates of Pseudomonas taiwnensis and 2 Pseudomonas plecoglossicida had bla_VIM-2, and 22 isolates of P. aeruginosa and one Pseudomonas otitidis had bla_IMP-6. These MBL genes were all in class 1 integron. The size of class 1 integron with bla_VIM-2 ranged from 3.5 kb to 5.5 kb in clinical isolates of Pseudomonas spp. including P. aeruginosa. bla_VIM-2 was most often located first in the class 1 integron, sometimes in the second or third position, and these integrons often had aacA4 or aadA1. Strict infection control measures are needed to more effectively prevent further spread of these MBL-producing Pseudomonas spp. In addition, MBL-producing Pseudomonas spp. is expected to continue to spread in various countries and regions.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.270-273
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• 1994

Pseudomonas putida 3SK, which was constructed by the conjugal transfet of the $CAM::TOL^{*}$ plasmid of Pseudomonas putida CSnA and the NAH plasmid of Pseudomonas putida KCTC 2403 into n-alkane assimilating Pseudomonas putida KCTC 2405, showed a broad degradation spectrum and floc-forming ability. This strain degraded m-toluic acid, naphthalene, camphor and decane simultaneously. $Hg^{2+}$ at the concentration of 1 ppm in the minimal medium could not inhibit the growth of this strain. The degradation of m-toluic acid by Pseudomonas putida 3SK was not repressed by the easily utilizable compounds, such as glucose and succinate. But, the addition of formalin inhibited the growth of Pseudomonas putida 3SK. After the cultivation of this strain on the artificial wastewater containing m-toluic acid, naphthalene, camphor and decane for 24 hr, the initial COD value (1500) of the artificial wastewater was declined to 300.

• 식물병연구
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• 제6권1호
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• pp.1-5
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• 2000

• 대한임상검사과학회지
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• 제39권3호
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• pp.167-177
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• 2007

From the total 121,294 clinical materials submitted to the Department of Laboratory Medicine of "C" hospital from December 1, 2004 to November 30, 2006, 3,408 Pseudomonas spp. were isolated. The isolation frequencies of Pseudomonas spp. were as follows, P. aeruginosa 95.5%, P. putida 2.5%, P. fluorescens 0.8%, along with low frequencies of P. luteola, P. alcaligenes, P. stutzeri, P. oryzihabitants, P. mendocina and unidentified Pseudomonas species. The isolation rates of Pseudomonas spp. according to season and sex were evenly distributed. The isolated frequency of Pseudomonas spp. in male was two times higher than that of in female showing significantly more male patients in surgical areas and more female patients in internal areas (p<0.001). In monthly analysis, Pseudomonas spp. were the most frequently isolated in July (10.4%), but lowest in February (5.6%). Half of Pseudomonas spp. were isolated from sputum (48.2%). In the susceptibility analysis of Pseudomonas spp. by VITEK II AST cards, the Pseudomonas spp showing higher susceptibility against antimicrobial agents were piperacillin/tazobactam (82.7%) in P. aeruginosa; amikacin (84.7%), colistin (83.3%) in P. putida; and amikacin (96.3%), cefepime (87.5%), ceftazidime (87.5%) ciprofloxacin (92.3%), colistin (88.5%) gentamicin (96.2%), isepamicin (96,1%), meropenem (92.3%), netilmicin (96.0%), piperacillin/ tazobactam (95.4%) and tobramycin (92.6%) in P. fluorescens.

• 한국해양학회지
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• 제28권3호
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• pp.186-191
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• 1993

우리 나라 남해 연안수역 특히 마산만에서 초봄인 3~4월에 와편모조류로서는 가장 빨리 적조를 일으키는 종인 Xcrippsiella trochoidea와 해양생태계에서 분해와 물 질순환에 중요한 역할을 하고 있는 해양세균과의 상호관계를 규명하기 위해서 마산만의 저니에서 분리한 X. trochidea의 cyst를 발아시켜 배양하고 배양액에서 공존하는 세균을 분리하였다. 분리된 세균 중 우점종인 Pseudomonas spp.를 S. trochoide과 함께 천연멸균해수에 투여한 후 지방산 조성의 변화를 분석하여 비교하였다. 그 결과 C_{16:1},{\;}C_{16:0}$의 조성이 상대적으로 증가하였다. 특히 S. trochoidea 와 Pseudomonas spp.의 동시 배양이 $C_{20:5}과{\;}C_{18:0}$의 두 지방산은 S. trochoidea 에서는 오히려 증가하였으나 Pseudomonas spp.에서는 오히려 감소되어, 이 두종의 지방산 대사과정이 서로 연관된다고 추정하였다.

• Applied Biological Chemistry
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• 제35권1호
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• pp.30-35
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• 1992

Polyvinyl alcohol(PVA)을 탄소원으로 이용할 수 있는 세균 J2W 균주와 J2Y균주를 토양으로 부터 분리하였다. 분리된 이들 균주는 각각 별도의 순수배양으로는 PVA를 이용할 수 없었으나 이들 균주를 혼합배양 하였을 경우는 PVA를 분해 이용할 수 있었으며, 또한 PVA의 중합도(중합도 500, 1500, 2000)에 관계없이 이용할 수 있었다. 이들 두 균주는 다른 PVA 이용 공생균주 Pseudomonas PW와 Pseudomonas G5Y와 재구성하여 혼합배양 하였을 때 J2Y균주와 Pseudomonas PW, J2W 균주와 Pseudomonas G5Y 균주와의 혼합배양에서는 PVA를 이용할 수 있었다. 이들 두 균주는 동정 결과 J2W균주는 Pseudomons pseudomallei 근연균으로, J2Y는 Xanthomonas campestris 근연균으로 동정되었다.