Browse > Article
http://dx.doi.org/10.5322/JES.2005.14.10.955

Isolation and Nitrate Reduction Characteristics of Aerobic Denitrifier Pseudomonas sp. DN-9  

Cho Sun-Ja (Department of Microbiology, Pusan National University)
Jung Yong-Ju (Department of Microbiology, Pusan National University)
Lee Sang-Joon (Department of Microbiology, Pusan National University)
Publication Information
Journal of Environmental Science International / v.14, no.10, 2005 , pp. 955-963 More about this Journal
Abstract
From sludge of S municipal wastewater treatment plant in Busan, Korea, we isolated the denitrifier DN-9 which showed the ability of denitrification under aerobic conditionby the color change and gas formation in liquid culture with Giltay medium. The isolated strain was identified as Pseudomonas sp. DN-9 on the basis of the morphological, physiological, biochemical and nucleotide sequence analysis of l6S rRNA. The isolated strain, Pseudomonas sp. DN-9, has cytochrome $cd_1$, nirS of nitrite reductase. By the co-existance of additional ammonium and nitrate ion, the strain was not affected largely on growth in SL series broth. It seemed the result of denitrification. Although Pseudomonas sp. DN-9 has a good nitrate reduction activity under aerobic condition, the activity is less than Pseudomonas stutzeri in same cultivation condition. However, Escherichia coli had little the activity of aerobic denitrification and Pseudomonas putida showed lower activity of aerobic denitrification than Pseudomonas sp. DN-9 and Pseudomonas stutzeri in this study.
Keywords
Aerobic denitrifier; Nitrate reduction; Pseudomonas sp.;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Huang, H. K. and S. K. Tseng, 2001, Nitrate reduction by Citrobacter diversus under aerobic environment, Appl. Microbiol. biotechnol., 55, 90-94   DOI   ScienceOn
2 Gupta, A. B., 1997, Thiospaera pantotropha: a sulphur bacterium capable of simutaneous heterotrophic nitrification and aerobic denitrification, Enzyme Microb. Tech., 21, 589-595   DOI   ScienceOn
3 Patureau, D., N. Bernet, T. Bouchez, P. Dabert, J. P. Delgenes and R. Moletta, 1998, Biological nitrogen removal in a single aerobic reactor by association of a nitrifying ecosystem to an aerobic denitrifier, Microvigula aerodenitrificans, J. Mol. Catal B-Enzym., 435-439   DOI   ScienceOn
4 Hwang, S. H., Y. H. Lee and M. H. Cho, 1999, Isolaiton and Characteristics of Denitrifying Pseudomonas CW4, J. Korean Soc. Biotechnol. Bioeng, 14(5), 616-620
5 Bang, D. Y., Y. K. Park, C. H. Lee, T. Noike and Y. Watanabe, 1998, Denitrification mechanism and characterization of denitrifiers in aerobic-denitrification biofilm, J. Korean Soc. Environmental Engineers, 361-370
6 Kwon, D. W., 2002, Isolation and characterization of denitrification bacteria, J. Korean Soc. Hygienic Sciences, 8(1), 25-30
7 Takaya, N., M. A. B. Catalan-Sakairi, Y. Sakaguchi, I. Kato, Z. Zhou and H. Shoun, 2003, Aerobic denitrifying bacteria that produce low levels of nitrous oxide, Appl. Environ. Microbiol., 69, 3152-3157   DOI   ScienceOn
8 Hartig, E. and W. G. Zumft, Kinetics of nirS expression (cytochrome $cd_{1}$ nitrite reductase) in Pseudomonas stutzeri during the transition from aerobic respiration to denitrification: evidence for a denitrification specific nitrate- and nitrite-responsive regulatory system, J. Bacteriol., 181(1), 161-166
9 Krieg, N. R. and J. G. Holt, 1984, Bergey's mannual of systematoic bacteriology, The Williams and Wilkims Co., Baltimore, USA
10 Cha, W. S., H. I. Choi, D. B. Lee and J. M. Cha, 2003, Isolation and characterization of denitrification bacteria, J. Korean Soc. Biotechnol. Bioeng, 18(6), 461-465
11 Yoon, C. H. and H. J. Jim, 2001, Decolorization characteristics of secondary treatment effluent of livestock wastewater by electrochemical process, Livestock Wastewater Symposium, Korea
12 Peter, J., V. D. Hoek and A. Klapwijk, 1987, Nitrate removal from ground water, Water Res., 21, 989-997   DOI   ScienceOn
13 Randall, C. W., J. L. Barnard and H. D. Stensel (editted), 1992, Design and retrofit of wastewater treatment plants for biological nutrient removal, Technomic Publishing Company, Inc., USA, 2-7pp
14 Cervantes, F. J., D. A. Rosa and J. Gomez, 2001, Nitrogen removal from wastewaters at low C/N ratios with ammonium and acetate as electron donors, Bioresource Technol., 79, 165-170   DOI   ScienceOn
15 이원선, 1999, The Evolution of advanced treatment for elimination nutrient from the municipal wastewater by biosorption, 서울산업대학교 산업대학원 환경공학과 공학석사 학위논문
16 U.S. EPA, 1993, Process design for manual nitrogen control, EPA, 625/R-23/010
17 Jetten, M. S. M., S. J. Horn and M. C. M. Loosdrecht, 1997, Towards a more sustainable municipal wastewater treatment system, Water Sci. Technol., 35, 171-180
18 Braker, G., J. Zhou, L. Wu, A. H. Devol and J. M. Tiedje, 2000, Nitrate reductase genes (nirK and nirS) as functional marders to investigate diversity of denitrifying bacteria in pacific northwest marine sediment communities, Appl. Environ. Microbiol., 66, 2096-2104   DOI   ScienceOn
19 Patreau, D., N. Bernet. J. P. Delgenes and R. Moletta, 2000, Effect of dissolved oxygen and carbon-nitrogen loads on denitrification by an aerobic consortium, Appl. Microbiol. Biotechnol., 54, 535-542   DOI   ScienceOn
20 Sistrom, W. R., 1962, The kinetics of the synthesis of photopigments in Rhodobacter sphaeroides, J. Gen. Microbiol., 28, 607-616   DOI   ScienceOn
21 Robertson, L. A., E. W. J. Niel, R. A. M. Torremans and J. G. Keunen, 1988, Simultanous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha, Appl. Environ. Microbiol., 54, 2812-2818