• Archives of Plastic Surgery
• /
• v.36 no.5
• /
• pp.611-616
• /
• 2009

Purpose: The rate of fistulas occuring followed by resection of oral cavity, oropharyngeal, hypopharyngeal, and laryngeal cancer are reported to be 9 ~ 23% according to various documents. Neglected treatment of the fistula can result in a setback in proper treatment with restrictions in oral intake leading to delayed return to daily life. Furthurmore, in severe cases, it may injure important vessels and adjacent structures of the neck area. The author reviewed previously reported cases of treatment methods for fistulas recurring after diverse head and neck operations and with sharing the treatment experiments of our patients, we tried to present a treatment algorism for different fistula types. Methods: Our study was based on retrograde analysis of 64 patients who were clinically diagnosed with fistula after operation for cancer of the head and neck from 1997 to 2008 at Severance Hospital. Their primary sites of cancer were 8 oral cavity, 22 oropharynx, 25 hypopharynx, and 9 larynx. The patients were aged 45 to 75 years and the male to female ratio was 11 to 1. The patient's operation records and progress notes were evaluated for determination of degree of fistula and treatment methods. Results: Most fistulas were clinically suspected after postoperative 5 days and symptoms noted for detection of the fistula were erythema, purulent discharge, edema, tenderness, and fluctuation. The fistula was definitely diagnosed at postoperative 2 weeks with barium test and treatment method ranging from conservative management to operative procedure were applied to each patients. Total 21 patients were managed with conservative protocol. In 15 cases, direct repair of the fistula was done and more stable repair of the fistula was possible with using of TachoComb$^{(R)}$. Pharyngostoma was performed in 14 patients. Among them, 4 patients healed spontaneously, 5 patients were taken direct closure, 4 patients were taken pectoralis major musculocutaneous flap, and one patient was taken esophageal transfer. The other 14 patients were taken 11 pectoralis major musculocutaneous flaps and 3 free flaps without pharyngostoma formation. Conclusion: Fistula is a troublesome complication resulting after resection of head and neck cancer. Early detection and adequate treatment according to the period and condition of the fistula may prevent further complications and reduce the pain of the patient.

• Journal of Plant Biotechnology
• /
• v.41 no.4
• /
• pp.216-222
• /
• 2014

This study was carried out to develop an efficient transformation protocol via particle bombardment with PLBs (protocorm-like bodies) in Phalaenopsis. To achieve this aim, osmoticum treatment and an increasing shooting chances in particle bombardment process were applied for this study. In addition, pCAMBIA3301: ORE7 vector which contains a herbicide-resistance bar gene as a selectable marker and ORE7 gene as a gene of interests were employed. With regard to the increasing chances of shooting in particle bombardment, double shooting was the best results with 1.5 ~ 2.5 times higher than those of a single or triple shooting treatment in the productioon of PPT (D-L-phosphinothricin)-resistant PLBs. However, regeneration rate of shoots in double shooting was not high as a single shooting. Further, double shooting showed 35 ~ 40% higher than that of a single shooting in the frequency of browning. Regarding effects of different osmotic treatments, combination of 0.2 M sorbitol with 0.2 M mannitol showed the best results in transformation efficiency, regeneration of transformants and reduction of browning. Putative transgenic Phalaenopsis plants were analyzed by PCR analysis and confirmed the presence of bar and ORE 7 gene. Also, real-time PCR was conducted by using 21 transgenic plants and showed only 4 plants had one copy of transgene; whereas, the other 17 plants had more than 2 copies of transgene. Transgenic phalaenopsis plants produced in this study were transferred to pots and flowered normally without morphological variations in flower and leaf.

• Journal of Plant Biotechnology
• /
• v.47 no.1
• /
• pp.66-72
• /
• 2020

Alstroemeria plants were transformed by using an improved particle-gun-mediated transformation system. Friable embryogenic callus (FEC) induced from the leaves with axil tissues of Alstroemeria plant was used as the target tissue. Also, FEC was transformed with the bar gene was used as a selectable marker. In the case of plasmid pAHC25, 7.5% of the twice-bombarded FEC clumps showed blue foci, whereas the clumps with single bombardment showed only 2.3%. Additionally, a 90° rotation with double bombardment led to a higher frequency (6 times) of luciferase gene expression in PBL9780 than the control treatment. After 8 weeks of bombardment, more than 60 independent transgenic lines were obtained for pAHC25 and nearly 150 independent transgenic lines were obtained for PBL9780, all of which were resistant to PPT and demonstrated either GUS or luciferase activity. Regarding effect of osmotic treatment (0.2 M mannitol) with 7 different periods, the highest transient gene expression was obtained in 8 h before and 16 h after transformation in both pAHC25 and PBL9780. Compared with the control, at least three times more GUS foci and photons were observed in this treatment. With respect to different combinations of mannitol and sorbitol with 8 h before and 16 h after transformation, high numbers of transient and stable transgene expressions were observed in both 0.2 M mannitol and 0.2 M sorbitol used in the osmotic pre-culture. This combination showed the highest transformation efficiency in both pAHC25 (8.5%) and PBL9780 (14.5%). In the control treatment, only 10% of the FEC clumps produced somatic embryos. However, by using 0.2 M mannitol and 0.2 M sorbitol, the frequency of somatic embryos increased to 36.5% (pAHC25) and 22.9% (PBL9780). Of the somatic embryos produced, at least 60% germinated. Approximately 100 somatic embryos from these 210 independent transgenic lines from 2 plasmids developed into shoots, which were then transferred to the greenhouse. PCR analysis confirmed the presence of the bar gene. This is the report on the production of transgenic Alstroemeria plants by using particle bombardment with a high efficiency, thereby providing a new alternative for the transferring of gene of interests in Alstroemeria in the breeding program in the future.

• Journal of the Korean Society of Hazard Mitigation
• /
• v.9 no.5
• /
• pp.115-123
• /
• 2009

Integrated information to water quantity and quality is essential for planning water resources management as well as operating water-related infrastructures. Because data collection process including monitoring and maintenance is separated in different governmental agencies in Korea, integrating quantity and quality may provide effective and better management implementation. In this study, a number of suggestions regarding integration of water monitoring were concluded in terms of technological, legal and institutional implications. First, it is necessary to discuss national water monitoring plan, national water information management plan, agreement of standard terms of monitoring between ministries, and to revise the law(river law and water quality management law). Present stations for water monitoring should be used for both of quantity and quality monitoring. If station is newly installed or relocated, it is better that one single agency maintain monitoring frequency and data management as well. In addition, a monitoring protocol need to be agreed by each of parties. In order to develop integrated monitoring system, quality assurance of the collected data should be properly maintained. Since many purposes haven been concerned using of data analysis and assessment so far, it may not be easy to integrate water quantity and quality monitoring in a short period. However, the alternatives including enhancing institutional regulations and programs, advanced technology may promote an efficient integrated water monitoring.

• Journal of KIISE:Information Networking
• /
• v.33 no.6
• /
• pp.407-419
• /
• 2006

As substituting IPv6 network for all IPv4 network in a short time seems unattainable due to high cost and technical limitation, IPv4 and IPv6 are expected to coexist for a certain period of time. Under the co]existing environment of IPv4 and IPv6, interworking brings a number of extra security considerations even if it may have no security problem for each protocol respectively. Thus, the analysis and solutions for those various attacks toward IPv4/IPv6 interworking-related security are inevitably required for the sake of effective transition and settlement to IPv6. In this paper we carried out a proper rule of packet filtering for IPv6-in-IPv4 tunneling interworking environment to protect the IPv4/IPv6 interworking-related security attacks. Design and implementation of the packet filtering system suitable for IPv4/IPv6 tunneling environment in the form of Linux netfilter and ip6tables are also shown. Thru this study, the packet filtering system was found operating correctly ill the tunneling mechanism.

• Journal of Korea Multimedia Society
• /
• v.12 no.8
• /
• pp.1109-1119
• /
• 2009

WLAN(Wireless Local Area Network) standard is currently developing with increased wireless internet demand. Though existing IEEE 802.11e demonstrates that data rates exceed 54Mbps with assuring QoS(Quality of Service), wireless internet users can't be satisfied with real communication system. After IEEE 802.11e system, Study trends of IEEE 802.11n show two aspects, enhanced system throughput using aggregation among packets in MAC (Medium Access Control) layer, and better data rates adapting MIMO(Multiple-Input Multiple-Output) in PHY(Physical) layer. But, no one demonstrates IEEE 802.11n system performance results considering MAC and PHY connection. Therefore, this paper adapts MIMO in PHY layer for IEEE 802.11n system based on A-MPDU(Aggregation-MAC Protocol Data Unit) method in MAC layer considering MAC and PHY connection. SVD(Singular Value Decomposition) method with WLAN MIMO TGn Channel is used to analyze MIMO. Consequently, Simulation results show enhanced throughput and data rates compared to existing system. Also, We use Ns-2(Network Simulator-2) considering MAC and PHY connection for reality.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.4
• /
• pp.954-961
• /
• 2017

We investigated the purifications of PE-gal and CPN-gal, synthesized by transgalactosylation reaction using recombinant ${\beta}$-gal. The reaction mixture containing PE and PE-gal was first mixed with EA, and thereafter PE and PE-gal were distributed in two-phase (EA/water) system. In this system, PE and PE-gal was selectively moved into EA and water phase, respectively. Then, the water phase was collected, and silica gel chromatography was carried out using the collected water phase. Finally, we compared two purified PE-gal samples using HPLC and TLC analysis, in which the one was purified only by silica gel chromatography and the other was purified by EA extraction followed by silica gel chromatography. In the latter case, the residual PE was almost completely removed, whereas, in the former case, the residual PE was remained remarkably. Additionally, the purification yield of PE-gal was about 21% on the basis of mole. In the same purification protocol, CPN-gal was able to be purified using EA extraction followed by silica gel chromatography, in which the residual CPN was almost removed when CPN-gal was purified by EA extraction followed by silica gel chromatography.

• KIPS Transactions on Computer and Communication Systems
• /
• v.3 no.10
• /
• pp.323-328
• /
• 2014

ZigBee based on the most vulnerable part of u-Healthcare system that uses the ZigBee communication is the wireless section. ZigBee communication sectors to identify vulnerabilities in this paper, we propose to compensate. ZigBee has been raised from the existing vulnerabilities organize and ZigBee also uses the 64bit address that uniquely identifies a vulnerability that was defined as exposure. And to prevent the exposure of a unique identifying address was used to address a temporary identification. ZigBee security services, the proposed system during the Network Key for encryption only use one mechanism of Residential Mode is used. Residential Mode on all nodes of the entire network because they use a common key, the key is stolen, your network's security system at a time are at risk of collapse. Therefore, in order to guard against these risks to the security policy Network Key updated periodically depending on the method used to. The proposed evaluation and comparative analysis of the system were exposed in the existing system can hide the address that uniquely identifies a public key Network Key also updated periodically, so that leaks can occur due to reduced risk.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.16 no.6
• /
• pp.683-690
• /
• 2006

Reducing power consumption to extend network lifetime is one of the most important challenges in designing wireless sensor networks. One promising approach to conserving system energy is to keep only a minimal number of sensors active and put others into low-powered sleep mode, while the active sensors can maintain a connected covet set for the target area. The problem of computing such minimum working sensor set is NP-hard. In this paper, a centralized Voronoi tessellation (CVT) based approximate algorithm is proposed to construct the near optimal cover set. When sensor's communication radius is at least twice of its sensing radius, the covet set is connected at the same time; In case of sensor's communication radius is smaller than twice of its sensing radius, a connection scheme is proposed to calculate the assistant nodes needed for constructing the connectivity of the cover set. Finally, the performance of the proposed algorithm is evaluated through theoretical analysis and extensive numerical experiments. Experimental results show that the proposed algorithm outperforms the greedy algorithm in terms of the runtime and the size of the constructed connected cover set.

• Korean Journal of Breeding Science
• /
• v.41 no.3
• /
• pp.252-260
• /
• 2009

Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.