• 한국산학기술학회논문지
• /
• 제12권1호
• /
• pp.276-280
• /
• 2011

돼지 췌장에서 추출한 판크레아틴 효소들의 활성화 조건에 대해 조사하였다. 십이지장의 첨가는 췌장의 단백질분해효소와 지방분해효소의 활성화를 유도하였다. 췌장액에 10% 십이지장을 첨가하여 $30^{\circ}C$에서 90분간 반응시키거나 $25^{\circ}C$에서 4시간 반응시켰을때 췌장의 단백질분해효소와 지방분해효소의 활성화는 정점에 도달하였고, 전분분해효소의 활성에는 거의 영향을 주지 않았다. 2$5^{\circ}C$에서 4시간의 효소활성화, 원심분리, 아세톤침전 및 동결건조의 연속 공정으로 제조한 판크레아틴 효소들의 비활성도는 단백질분해효소 136 U/mg, 지방분해효소 170 U/mg 및 전분분해효소 400 U/mg으로 나타났다. 확립된 공정에 의해 제조된 판크레아틴은 USP 기준의 5.4배의 단백질분해효소, 58배의 지방분해효소 및 16배의 전분분해효소 활성을 보유한 고활성의 제품이었다.

• Journal of Microbiology
• /
• 제39권4호
• /
• pp.305-313
• /
• 2001

The spr D gene encoding Strptomyces griseus protease D(SGPD); a chymotrypsin-like proteae, was cloned from Strptomyces griseus IFO13350 and sequence. Most of the amino-acid sequence deduced from the nucleotide sequence is idential to that Strptomyces griseus IMRU3499 except that one amino acid has been deleted and Trp 369 has been substituted into Cys369 in the SGPD from S. griseus IFO13350 without affecting the protease activity. The spr D gene was overexpressed in Streptomyce liv-idans TK24 as a heterologous host. Various media with different compositions were also used to max-imize the productivity of SGPD inthe heterologous host. The SGPD productivity was best when the transformant S. lividans TK24 was cultivated in R2YE medium. The relative chymotrypsin activity of the culture broth measured with an artificial chromogenic substrate, N-scuccinyl-ala-ala-pro-phe-p-nitroanilide, was 16 units/ml. A high level of SGPD was also produced in YEME and SAAM medial but it was relatively lower that in R2YE medium and negligible amounts of SGPD were produced in GYE, GAE and Benedict media. The growth of S. lividans reacted the maximum level of cell mass at days 3 and 4 of the culture, but SGPD production started in the stationary phase of cell growth and kept increase in till the 10$^{th}$ day of culture in R2YE and YEME medium, but in GYE media the productivity reached maximum level at 8days of cultivation. The introduction of the spr D gene into S. lividans TK24 triggered biosyntheis of the pigmented antibiotic , actinorhodin, which implies some protease may paly a very improtant role in secondary-metabolite formation in sStreptomyces.

• The Journal of Advanced Prosthodontics
• /
• 제16권2호
• /
• pp.126-138
• /
• 2024

PURPOSE. The aim of this study was to evaluate the influence of different 3D dental resins, using a manufacturer recommended printer and a third-party printer, on cellular responses of human gingival cells. MATERIALS AND METHODS. Three NextDent resins (Denture 3D+, C&B MFH and Crowntec) were used to produce specimens on printers NextDent 5100 (groups ND, NC and NT, respectively) and Phrozen Sonic Mini 4K (groups PD, PC and PT, respectively). Human gingival fibroblasts were cultured and biocompatibility was evaluated on days 1, 3 and 7. IL-6 and IL-8 concentrations were evaluated at 3 days using ELISA. Surface roughness was evaluated by a contact profilometer. SEM and fluorescence micrographs were analyzed at days 1 and 7. Statistical analyses were performed using SPSS and mean differences were tested using ANOVA and post-hoc Tukey tests (P < .05). RESULTS. There was an increase in cellular viability after 7 days in groups PC and PT, when compared to group PD. ND group resulted in higher concentration of IL-6 when compared to PT group. SEM and fluorescence micrographs showed less adhesion and thinner morphology of fibroblasts from group PD. No significant differences were found regarding surface roughness. CONCLUSION. The use of different printers or resins did not seem to influence surface roughness. NextDent 5100 and Phrozen Sonic Mini 4K produced resins with similar cellular responses in human gingival fibroblasts. However, Denture 3D+ resin resulted in significantly lower biocompatibility, when compared to C&B MFH and Crowntec resins. Further testing is required to support its long-term use, required for complete dentures.