• Asian-Australasian Journal of Animal Sciences
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• 제9권6호
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• pp.643-646
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• 1996

Experiments were conducted to determine potassium (K) requirement of mule ducklings. One-day-old ducklings with equal number of both sexes were fed diets containing graded levels of K for three weeks. In experiment 1, corn-soybean meal diet (CP 18.7%, ME 2,890 kcal/kg, K 0.80%) was used. The addition of K (0.00, 0.10, 0.20, 0.30, 0.40%) to the diet by potassium carbonate resulted in a decrease in weight gain and feed efficiency with the group fed on basal diet having the best performance. This means that the existing K in the basal diet is adequate for the growth of ducklings. In experiment 2, low K corn-isolated soy protein diet (K, 0.19%) was used. The supplementation of K (0.00, 0.16, 0.32, 0.48, 0.64%) resulted in an increase in weight gain and feed efficiency with the control group having the lowest performance. The minimum K requirement was found to be 0.49% for both maximum growth and best feed efficiency as determined by using bent-stick model.

• Journal of Nutrition and Health
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• 제15권4호
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• pp.290-300
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• 1982

The study deals with the empirical research on the condition of nutrient intake of low income class which be represented by urban slum and rural area, with the analysis of the factors which might influence on the prevalent condition of nutrient- intake. The method of the research was based on the spot-survey with questionaires. The result is that the levels of nutrient-intake are below the standard requirement level of nutrients in both of urban slum and rural area. The level of nutrient intake in urban slum lies in approximately 50% of the standard requirement level and 80% of the standard requirement level in rural area. The extent of malnutrition was explained in terms of the amount of calorie, protein, calcium and iron. More than half of the population in the community are below the standard requirement level of the nutrient- intake. The problem of malnutrition was serious in urban slum than in rural areas, which made a good contrast with the result of Peru study. Deficiency in calcium was most serious. The factor analysis of the prevalent condition of malnutrition in low class suggests that 1) The function of local market in supplying food is not so effective in the sense that the quality of the foods purchased id the local market is poor. 2) Low level of knowledge, the consequent ignorance and the indifference to the nutrition and the low income led to malnutrition. The level of income and the education were significantly correlated to the nutrient-intake.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1397-1406
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• 2019

Objective: Feed energy required for pigs is first prioritized to meet maintenance costs. Additional energy intake in excess of the energy requirement for maintenance is retained as protein and fat in the body, leading to weight gain. The objective of this study was to estimate the metabolizable energy requirements for maintenance ($ME_m$) by regressing body weight (BW) gain against metabolizable energy intake (MEI) in growing pigs. Methods: Thirty-six growing pigs ($26.3{\pm}1.7kg$) were allotted to 1 of 6 treatments with 6 replicates per treatment in a randomized complete block design. Treatments were 6 feeding levels which were calculated as 50%, 60%, 70%, 80%, 90%, or 100% of the estimated ad libitum MEI ($2,400kJ/kg\;BW^{0.60}\;d$). All pigs were individually housed in metabolism crates for 30 d and weighed every 5 d. Moreover, each pig from each treatment was placed in the open-circuit respiration chambers to measure heat production (HP) and energy retained as protein ($RE_p$) and fat ($RE_f$) every 5 d. Serum biochemical parameters of pigs were analyzed at the end of the experiment. Results: The average daily gain (ADG) and HP as well as the $RE_p$ and $RE_f$ linearly increased with increasing feed intake (p<0.010). ${\beta}$-hydroxybutyrate concentration of serum tended to increase with increasing feed intake (p = 0.080). The regression equations of MEI on ADG were MEI, $kJ/kg\;BW^{0.60}\;d=1.88{\times}ADG$, g/d+782 ($R^2=0.86$) and $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$. Protein retention of growing pigs would be positive while REf would be negative at this feeding level via regression equations of $RE_p$ and $RE_f$ on MEI. Conclusion: The $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$ in current experiment. Furthermore, growing pigs will deposit protein and oxidize fat if provided feed at the estimated maintenance level.

• 한국식생활문화학회지
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• 제11권5호
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• pp.643-649
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• 1996

This study was performed to investigate the adequacy of dietary fat and protein intakes among female college students. Daily intakes of energy, fat, protein, major amino acids and other nutrients were measured in 52 female college students. Daily energy intake was 75.8% of the recommended intake. Fat and protein consist 19.2% and 16.7% of the total calorie, respectively. The average protein consumption per day was 105% of the recommended intake. Essential amino acids intakes were more than the recommended amounts which appears in the 6th edition of Recommended Dietary Allowances for Koreans. However, when the intake of each essential amino acid was compared to the recommended amino acid requirement pattern, these subjects did not meet the estimated requirements. There was a highly significant correlation between daily protein intake and lipid intake implying the major sources of protein in the diet were also major sources of fat. Daily intakes of dietary fiber, vitamin C, iron, and phosphorous were above the recommended levels of intake. However, blood hemoglobin concentration was marginal indicating dietary iron consumption is not a good marker for iron status. Also, calcium intake was only 63.5% of the recommended intake. Therefore, these results imply that main problems for these subjects are low energy consumption, low calcium intake, and the quality of protein. However, as opposed to the hypothesis, the main energy sources were not the food items high in saturated fats such as instant foods, which should be emphasized further.

• Journal of Nutrition and Health
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• 제26권9호
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• pp.1033-1048
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• 1993

This study was designed to describe the effect of the protein quality at different intake level of protein on the protein metabolism in the whole body of growing pigs with a simulation model. Varying to the protein level in feeds, four simulations were conducted. The feed protein level, represented as proportions of digestible protein to the metabolic energy (DP/ME, g/MJ), were 6-8, 11-13, 17-19, and 23-25 DP/ME, respectively. Two protein quality and six weeks of growth time were used at each simulation. The objective function for the simulations was protein deposition in the whole body, which was calculated from the experimental results. The parameters in the simulation were determined by the parameter estimation technique. The results obtained from the simulation were as follows: The protein synthesis and breakdown rates(g/day) in the whole body was increased with the increase of protein quality only at lower or required level of protein intake. They showed a parallel behavior in the course of growth, irrespective of quality and level of feed protein intake. The simulated protein deposition and protein synthesis showed a linear relationship between them at different protein quality and level. The affinity parameter showed a linear relationship between them at different protein quality and level. The affinity parameter showed that arginine, tryptophan and isoleucine were more efficient in the stimulation ofbody protein synthesis. Lysine and phenylalanine+tyrosine were less efficient. The oxidation parameter showed that histidine, pheyalanine+tyrosine were less efficient. The oxidation parameter showed that histidine, phenyalanine+tyrosine, and methionine+cystine were oxidized in larger magnitude than lysine and threonine. The oxidation parameter of most amino acids increased with the increase of protein intake beyond the requirement level, but not any more at highest protein intake level. Finally it was found that the improvement of feed protein quality at the lower or required level of protein intake increase protein deposition through a parallel increase of protein synthesis and breakdown.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.759-769
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• 2002

Mutated forms of ras are found in many human tumors and the rate of incidence is significantly higher in colon and pancreatic cancers. The protein product from the ras oncogene is a small G-protein, $p21^{ras}{\;}(Ras)$ that is known to playa key role in the signal transduction cascade and cell differentiation and proliferation. Mutated Ras is unable to regulate itself and remains constantly activated, leading to uncontrolled cell growth. The function of Ras in signal transduction requires its location near the growth factor receptor at the cell membrane. However, Ras does not have a transmembrane domain. Ras requires farnesylation to increase its hydrophobicity and subsequent plasma membrane association for its transforming activity. This key post-translational modification is catalyzed by the enzyme Ras farnesyltransferase (FTase), which transfers a farnesyl group from farnesylpyrophosphate to the C-terminal cysteine of the Ras protein. The requirement has focused attention on FTase as a target for therapeutic intervention. Selective inhibition of FTase will prevent Ras protein from association with the plasma membrane, leading to a disruption of oncogenic Ras function.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.381-387
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• 1999

Two experiments were conducted to subdivide threonine (exp. 1) and glycine (exp. 2) requirements of broilers into maintenance and growth requirements. Purified diets containing five graded levels of threonine (exp. 1) and glycine (exp. 2) were fed to growing chicks to estimate threonine (exp. 1) and glycine (exp. 2) requirements for growth and maintenance. A model developed to divide threonine requirement for maintenance from that for growth yielded a requirement for growth of 8.946 mg/g weight gain and 0.341 mg/mg N gain; the maintenance requirement was 0.033 or 0.030 mg per unit of metabolic body size $(Wg^{0.75})$. The plateau of plasma threonine concentration occurred at 279.4 mg threonine intake/day. The total threonine requirement was 289.1 mg/day or 0.69% of the diet, 294.1 mg/day or 0.71% of the diet based on weight gain and nitrogen gain responses, respectively. These estimates were in close agreement with previous estimates of threonine requirements. From the relationship of weight gain to N gain, 5.46% of the retained protein consisted of threonine; the reported threonine content of chick muscle was 4.02%. The glycine requirement for maintenance could not be determined due to failure to obtain data allowing extrapolation to zero response. However, ADG increased slightly up to 0.56% glycine.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.190.2-190
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• 1997

No Abstract, See Full Text

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1172-1180
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• 2013

Two experiments were conducted to determine the standardized ileal digestible (SID) lysine (Lys) requirement and the ideal SID threonine (Thr) to Lys ratio for finishing barrows. In Exp. 1, 120 barrows with an average body weight of $72.8{\pm}3.6$ kg were allotted to one of six dietary treatments in a randomized complete block design conducted for 35 d. Each diet was fed to five pens of pigs containing four barrows. A normal crude protein (CP) diet providing 15.3% CP and 0.71% SID Lys and five low CP diets providing 12% CP with SID Lys concentrations of 0.51, 0.61, 0.71, 0.81 and 0.91% were formulated. Increasing the SID Lys content of the diet resulted in an increase in weight gain (linear effect p = 0.04 and quadratic effect p = 0.08) and an improvement in feed conversion ratio (FCR) (linear effect p = 0.02 and quadratic effect p = 0.02). For weight gain and FCR, the estimated SID Lys requirement of finishing barrows were 0.71 and 0.71% (linear broken-line analysis), 0.79 and 0.78% (quadratic analysis), respectively. Exp. 2 was a 26 d dose-response study using SID Thr to Lys ratios of 0.56, 0.61, 0.67, 0.72 and 0.77. A total of 138 barrows weighing $72.5{\pm}4.4$ kg were randomly allotted to receive one of the five diets. All diets were formulated to contain 0.61% SID Lys (10.5% CP), which is slightly lower than the pig's requirement. Weight gain was quadratically (p = 0.03) affected by SID Thr to Lys ratio while FCR was linearly improved (p = 0.02). The SID Thr to Lys ratios for maximal weight gain and minimal FCR and serum urea nitrogen (SUN) were 0.67, 0.71 and 0.64 using a linear broken-line model and 0.68, 0.78 and 0.70 using a quadratic model, respectively. Based on the estimates obtained from the broken-line and quadratic analysis, we concluded that the dietary SID Lys requirement for both maximum weight gain and minimum FCR was 0.75%, and an optimum SID Thr to Lys ratio was 0.68 to maximize weight gain, 0.75 to optimize FCR and 0.67 to minimize SUN for finishing barrows.

• Applied Biological Chemistry
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• 제24권2호
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• pp.94-100
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• 1981

콩나물제조중(製造中) 단백질(蛋白質)의 영양가(營養價)를 화학적(化學的)으로 평가(評價)하기 위해서 대두중(大豆中)의 아미노산조성(組成)과 이들대두(大豆)가 발아하여 콩나물로 자라는 동안의 아미노산의 변화(變化)를 분석(分析)하였다. 콩나물제조중(製造中) 총(總)아미노산의 변화(變化)를 분석(分析)하였다. 콩나물제조중(製造中) 총(總)아미노산의 변화(變化)는 2일(日)까지는 거의 함량변화(變化)가 없으나 그 이후(以後)부터는 감소(減少)되었고 부위별(部位別)로는 자엽부(子葉部)에서는 감소(減少)되나 배유부(胚乳部)에서는 증가(增加)되었다. 총(總)아미노산에 대(對)한 필수(必修)아미노산의 비(比)는 4일(日) 이후(以後)에 급격(急激)히 감소(減少)되는데 특히 배유부(胚乳部)에서 더 크게 감소(減少)되었다. 여러 아미노산중(中) 특히 aspartic acid의 증가(增加)가 크고 glutamic acid의 감소(減少)가 현저하였다. 총(總)아미노산조성(組成)에 의하여 chemical score를 구(求)하면 A/E와 A/T치(値)는 불규칙(不規則)하게 나타났으나 대두(大豆)와 콩나물 다같이 대체적(大體的)으로 낮은 치(値)를 보였으며 제한(制限)아미노산은 methionin이었다. FAO refernce protein과 requirement pattern을 기준으로하여 구(求)한 chemical score의 값은 2일(日)콩나물>콩>4일(日)콩나물>8일(日)콩나물>6일(日)콩나물의 순(順)으로 나타났고 반면에 essential amino acid index와 requirement index로는 2일(日) 콩나물>콩>4일(日)콩나물>6일(日)콩나물>8일(日)콩나물의 순(順)으로 나타났다.