• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.184-190
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• 2004

The influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (${\beta}$-glucuronidase) and the extracellularly homologous protein (${\alpha}$-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae.

• 공업화학
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• 제17권5호
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• pp.509-516
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• 2006

Rushton turbine, paddle and propeller 임펠러 등 표준적인 임펠러가 부착된 기-액-고 3상교반조에서 고-액간 물질이동계수 $k_L$를 측정하였다. 표준적인 임펠러들에 대한 부유화한계회전수 $N_{js}$는 임펠러 높이와 가스유속에 따라 변했다. 입자부유가 통기에 의존한 Rushton turbine 임펠러의 물질이동계수는 Pgv에만 상관하였다. Rushton turbine, paddle and propeller 임펠러에 대한 물질이동계수 $k_L$은 임펠러의 종류에 의하지 않고 임펠러 위치에 영향을 받았다.