• BLOOD RESEARCH
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• v.53 no.4
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• pp.320-324
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• 2018

Background Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities. Methods MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH). Results MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients. Conclusion Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.87-93
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• 2021

As the portion of modern weapons systems equipped with electronic components increases, the presence of embedded software has also increased, subsequently drawing interest in quality management and development methods. Unlike mechanical systems, software is logically intangible, hard to troubleshoot, and hard to reuse, making it hard to utilize limited resources when exposed to failures. The United States had successful results in establishing the Capability Maturity Model (CMM) by studying and applying separate processes for development and quality management of defense software. South Korea has also established, and utilizes, a standard of development and quality management for defense software based on international standards. However, some say those standards still remain at a basic level, and should be modified along with the progress in software. If the standard stays at a basic level, compared to software progression, the cost to reuse and restore resources will increase exponentially. This paper discusses improvement in the test processes for defense software through the Test Maturity Model-integration (TMMi)-derived from the CMM-and presents a blueprint for defense software quality management.

• Journal of Korean Neurosurgical Society
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• v.64 no.4
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• pp.585-591
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• 2021

Objective : Filum transection is one of the most commonly performed operative procedure in pediatric neurosurgery. However, the clinical and pathological features as well as the surgical indication are not well-established. This study aimed to analyze the characteristics of patients who underwent transection of the filum during the last 10 years in a single institute. Methods : A total of 82 patients underwent transection of the filum during the period. As a general rule, we performed the transection in patients who are symptomatic or have abnormality in the urologic or neuromuscular evaluations. There were exceptions as asymptomatic patients who only fit the definition of thickened filum (width greater than 2.0 mm or conus level below L3 vertebral body) were operated by parent's wish or surgeon's preference according to radiological findings, etc. Results : Seventy-six out of 82 patients had fibrous tissue in the pathologic specimen of filum. Interestingly, patients who had glial cells were more correlated with no preoperative syrinx, and no progression of syrinx even for those who did have syrinx initially. Also, larger percentage of symptomatic patients had peripheral nerve twigs than asymptomatic patients. No difference in conus level or thickness of filum was found between patients with or without preoperative syrinx. Significantly more patients with syrinx (56%) were chosen to be operated without any symptom or abnormality in study i.e., solely based on radiological findings than those without syrinx (21%). The surgical outcome for syrinx was favorable, as all but one patient had either improved or static syrinx. The exceptional case had increase in size due to the upward displacement of the proximal end of the cut filum. Conclusion : This study evaluated the pathological, clinical, radiological features of patients who underwent transection of the filum. Interesting correlations between pathological findings and clinical features were found. Excellent outcome regarding preoperative syrinx was also shown.

• Journal of Microbiology and Biotechnology
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• v.31 no.6
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• pp.867-874
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• 2021

Epalrestat (EPS) is a brain penetrant aldose reductase inhibitor, an approved drug currently used for the treatment of diabetic neuropathy. At near-plasma concentration, EPS induces glutathione biosynthesis, which in turn reduces oxidative stress in the neuronal cells. In this study, we found that EPS reduces neurodegeneration by inhibiting reactive oxygen species (ROS)-induced oxidative injury, mitochondrial membrane damage, apoptosis and tauopathy. EPS treatment up to 50 µM did not show any toxic effect on SH-SY5Y cell line (neuroblastoma cells). However, we observed toxic effect at a concentration of 100 µM and above. At 50 µM concentration, EPS showed better antioxidant activity against H₂O₂ (100 µM)-induced cytotoxicity, ROS formation and mitochondrial membrane damage in retinoic acid-differentiated SH-SY5Y cell line. Furthermore, our study revealed that 50 µM of EPS concentration reduced the glycogen synthase kinase-3 β (GSK3-β) expression and total tau protein level in H₂O₂ (100 µM)-treated cells. Findings from this study confirms the therapeutic efficacy of EPS on regulating Alzheimer's disease (AD) by regulating GSK3-β and total tau proteins phosphorylation, which helped to restore the cellular viability. This process could also reduce toxic fibrillary tangle formation and disease progression of AD. Therefore, it is our view that an optimal concentration of EPS therapy could decrease AD pathology by reducing tau phosphorylation through regulating the expression level of GSK3-β.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.5
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• pp.662-669
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• 2022

The purpose of this study is to test the effects of electrolyzed water treatment on dried-laver Pyropia sp. processing facilities to control microbial contamination. Following the progression of the process to the next step, as well as during the lapse between process operating hours, the contamination level of total viable cell counts (TVC) and total coliform (TC) of laver increased. The TVC increased during the aging step, and after the molding-drying steps were completed, it increased by approximately 2.0 log CFU (colony forming unit)/g. Freshwater used for processing in April had a TVC of 4.31 log CFU/mL, which was more polluted than 2.61 log CFU/mL of seawater. Electrolyzed water was used to treat the sponge used in the laver-molding process, which resulted in a 2 log CFU/mL decrease.The TVC of dried-laver decreased by 1 to 2 log CFU/g when electrolyzed water was applied to the process. In conclusion, application of electrolyzed water in dried-laver processing was shown to be effective in reducing the microbiological contamination of the final product.

• Journal of Microbiology and Biotechnology
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• v.32 no.7
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• pp.938-948
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• 2022

Gastric cancers (GC) are generally malignant tumors, occurring with high incidence and threatening public health around the world. Circular RNAs (circRNAs) play crucial roles in modulating various cancers, including GC. However, the functions of circRNAs and their regulatory mechanism in colorectal cancer (CRC) remain largely unknown. This study focuses on both the role of circCOL1A2 in CRC progression as well as its downstream molecular mechanism. Quantitative polymerase chain reaction (qPCR) and western blot were adopted for gene expression analysis. Functional experiments were performed to study the biological functions. Fluorescence in situ hybridization (FISH) and subcellular fraction assays were employed to detect the subcellular distribution. Luciferase reporter, RNA-binding protein immunoprecipitation (RIP), co-immunoprecipitation (Co-IP), RNA pull-down, and immunofluorescence (IF) and immunoprecipitation (IP) assays were used to explore the underlying mechanisms. Our results found circCOL1A2 to be not only upregulated in GC cells, but that it also propels the migration and invasion of GC cells. CircCOL1A2 functions as a competing endogenous RNA (ceRNA) by sequestering microRNA-1286 (miR-1286) to modulate ubiquitin-specific peptidase 10 (USP10), which in turn spurs the migration and invasion of GC cells by regulating RFC2. In sum, CircCOL1A2 sponges miR-1286 to promote cell invasion and migration of GC by elevating the expression of USP10 to downregulate the level of RFC2 ubiquitination. Our study offers a potential novel target for the early diagnosis and treatment of GC.

• Journal of Convergence for Information Technology
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• v.12 no.5
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• pp.271-277
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• 2022

In this study, the effect of the anatomical practice extracurricular program on the major efficacy and satisfaction of medical health students was investigated. The research tool used a major efficacy survey, a program satisfaction survey, the major efficacy survey compared and analyzed the improvement before and after participation in anatomy practice and the satisfaction survey between anatomy pictures after completion of the extracurricular program. As a result of comparative analysis between the two groups, the effect on major efficacy was significant after the program progression, and in the case of satisfaction, the satisfaction of the anatomical practice group was higher than that of the anatomical picture group. Through these research results, when operating an anatomical practice extracurricular program, it is thought that operating an anatomical practice program rather than creating an anatomical picture improves the major efficacy and satisfaction score.

• IMMUNE NETWORK
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• v.1 no.3
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• pp.260-265
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• 2001

Transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$) is a multipotent growth factor affecting development, homeostasis and tissue repair. Many kinds of malignant tissues were reported to overexpress transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$) gene. However, a little work has been done on the circulating $TGF-{\beta}1$ and the association of $TGF-{\beta}1$ with progression in patients with malignant tumors. In this study, we measured the plasma level of $TGF-{\beta}1$ in gastric cancer and prostate cancer patients and evaluated the utility of plasma $TGF-{\beta}1$ as a possible tumor marker. We used Enzyme-linked immunosorbent assay (ELISA) system in order to measure plasma $TGF-{\beta}1$ level in 134 gastric cancer patients, 50 prostate cancer patients and 290 normal controls. And the tumor marker, carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), was compared with $TGF-{\beta}1$ in the aspects of sensitivity and specificity. The mean plasma $TGF-{\beta}1$ levels were $1.219{\pm}0.834$ (0.272-5.772) ng/mL in normal controls, $5.964{\pm}3.218$ (0.845-18.124) ng/mL in gastric cancer and $4.140{\pm}2.345$ (1.108-13.302) ng/mL in prostate cancer. In gastric cancer patients difference in plasma $TGF-{\beta}1$ level was not detected according to cancer stage. In comparison with other tumor marker (CEA, PSA) $TGF-{\beta}1$ is more potent in sensitivity. These results indicate that the plasma $TGF-{\beta}1$ level can be a potent tumor marker in gastric cancer and prostate cancer.

• 한국노년학
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• v.41 no.3
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• pp.295-308
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• 2021

This study is a descriptive study conducted to find out the predictive factors according to the level of the frailty of the communitydwelling older adult in a rural area. Data were collected from 400 older adults aged 65 years or older living in rural areas of Gyeongsangnam-do from October 2019 to March 2020. Data were analyzed using logistic regression to examine the predictive factors according to the level of frailty. The results showed that 27.8% for robust older adults, 30.9% for pre-frailty older adults, and 41.3% for frailty older adults. As a result of analyzing the predictive factors according to the level of frailty, the predictors from the robust stage to the pre-frailty stage were grip strength, nutritional status, and depression. The predictive factors for entering the pre-frailty stage into the frailty stage were gender, nutritional status, physical performance ability, and depression. Also, it was found that the predictive factors for entering from the robust stage to the frailty stage were sex, occupation, nutritional status, physical performance ability, and depression. Through this study, it was possible to understand the level of the frailty of the older adults living in rural communities and the effects of multidimensional variables. These results can be used as basic data necessary to find a way to prevent and manage the progression of frailty among older adults in rural areas.

• Information Systems Review
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• v.23 no.4
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• pp.23-43
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• 2021

Due to the recent transition to a non-face-to-face society, many organizations are quickly adapting to foster a smart work environment. The introduction of smart work does not simply end with incorporating ICT systems or solutions into business models since fundamental factors such as forms of employment and work styles need to be in line with the progression of technological advances. However, previous studies regarding smart work focus on improvements in productivity and efficiency from a technology acceptance perspective. Therefore, there is a lack of discussion on innovation resistance from employees and management control when ICT systems are introduced into the workplace. This study empirically analyzes the moderating effects of the organizational control method for employees and innovation resistance within a smart work environment. Additionally, this study aims to identify the structural characteristics that employees resist from an innovation resistance perspective when organizational innovation occurs. The empirical analysis of this study suggests that when smart work such as ICT technology is introduced into the workplace the level of innovation resistance decreases when there is a high level of relative advantage and self-efficacy, whereas the level of innovation resistance increases when there is a high level of use complexity. Moreover, this study revealed that the level of innovation resistance increases when the employees' behaviors were controlled. The results of this study intend to contribute to improving business management by suggesting factors worth considering when incorporating smart work into work places through a thorough case analysis.