• 한국축산식품학회지
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• 제42권6호
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• pp.1061-1073
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• 2022

Receptor activator of NF-kB ligand (RANKL) is known to play a major role in bone metabolism and the immune system, and its recombinant form has been expressed in bacterial systems for research since the last two decades. However, most of these recombinant forms are used after purification or directly using living cells. Here, there were cell extracts of recombinant Lactococcus lactis expressing mouse RANKL (mRANKL) used to evaluate its biological activity in mice. Mice were divided into three groups that were fed phosphate-buffered saline (PBS), wild-type L. lactis IL1403 (WT_CE), and recombinant L. lactis expressing mRANKL (mRANKL_CE). The small intestinal transcriptome and fecal microbiome were then profiled. The biological activity of mRANKL_CE was confirmed by studying RANK-RANKL signaling in vitro and in vivo. For small intestinal transcriptome, differentially expressed genes (DEGs) were identified in the mRANKL_CE group, and no DEGs were found in the WT_CE group. In the PBS vs. mRANKL_CE gene enrichment analysis, upregulated genes were enriched for heat shock protein binding, regulation of bone resorption, and calcium ion binding. In the gut microbiome analysis, there were no critical changes among the three groups. However, Lactobacillus and Sphingomonas were more abundant in the mRANKL_CE group than in the other two groups. Our results indicate that cell extracts of mRANKL_CE can play an effective role without a significant impact on the intestine. This strategy may be useful for the development of protein drugs.

• 스마트미디어저널
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• 제12권4호
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• pp.19-26
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• 2023

뉴로모픽 아키텍처 기반 하드웨어를 이용한 IoT 엣지 서비스는 단말 장치에서 지능형 처리를 수행할 수 있기 때문에 자율형 IoT 응용 지원에 적합하다. 그러나 IoT 개발자가 뉴로모픽 하드웨어에서 사용되는 SNN을 이해하기에는 어려움이 있다. 본 논문에서는 뉴로모픽 하드웨어의 제약조건을 고려하며 사용자의 요구 성능을 만족하는 SNN 모델 생성 기법을 제안한다. 제안 기법은 프로파일링된 데이터에서 최적의 SNN 모델 파라미터를 찾도록 전처리된 데이터로 사전 학습한 모델을 활용한다. 전체 탐색 기법과 비교 결과, 두 기법 모두 사용자 요구사항을 모두 만족하였지만, 제안 기법이 수행 시간 측면에서 더 좋은 성능을 보였다. 또한, 신규 하드웨어의 제약조건을 명확히 알지 못하더라도 새로운 하드웨어의 프로파일링된 데이터를 활용할 수 있으므로 높은 확장성을 제공할 수 있다.

• Nutrition Research and Practice
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• 제17권5호
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• pp.883-898
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• 2023

BACKGROUND/OBJECTIVES: Probiotics have been suggested as potent modulators of age-related disorders in immunological functions, yet little is known about sex-dependent effects of probiotic supplements. Therefore, we aimed to investigate sex-dependent effects of probiotics on profiles of the gut microbiota and peripheral immune cells in healthy older adults. SUBJECTS/METHODS: In a randomized, double-blind, placebo-controlled, multicenter trial, healthy elderly individuals ≥ 65 yrs old were administered probiotic capsules (or placebo) for 12 wk. Gut microbiota was analyzed using 16S rRNA gene sequencing and bioinformatic analyses. Peripheral immune cells were profiled using flow cytometry for lymphocytes (natural killer, B, CD4⁺ T, and CD8⁺ T cells), dendritic cells, monocytes, and their subpopulations. RESULTS: Compared with placebo, phylum Firmicutes was significantly reduced in the probiotic group in women, but not in men. At the genus level, sex-specific responses included reductions in the relative abundances of pro-inflammatory gut microbes, including Catabacter and unclassified_Coriobacteriales, and Burkholderia and unclassified Enterobacteriaceae, in men and women, respectively. Peripheral immune cell profiling analysis revealed that in men, probiotics significantly reduced the proportions of dendritic cells and CD14⁺ CD16^- monocytes; however, these effects were not observed in women. In contrast, the proportion of total CD4⁺ T cells was significantly reduced in women in the probiotic group. Additionally, serum lipopolysaccharide-binding protein levels showed a decreasing tendency that were positively associated with changes in gut bacteria, including Catabacter (ρ = 0.678, P < 0.05) and Burkholderia (ρ = 0.673, P < 0.05) in men and women, respectively. CONCLUSIONS: These results suggest that probiotic supplementation may reduce the incidence of inflammation-related diseases by regulating the profiles of the gut microbiota and peripheral immune cells in healthy elders in a sex-specific manner.

• Animal Bioscience
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• 제37권6호
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• pp.993-1000
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• 2024

Objective: This study was conducted to investigate the differential expression of the major histocompatibility complex (MHC) gene region in Eimeria-infected broiler. Methods: We profiled gene expression of Eimeria-infected and uninfected ceca of broilers sampled at 4, 7, and 21 days post-infection (dpi) using RNA sequencing. Differentially expressed genes (DEGs) between two sample groups were identified at each time point. DEGs located on chicken chromosome 16 were used for further analysis. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis was conducted for the functional annotation of DEGs. Results: Fourteen significant (false discovery rate <0.1) DEGs were identified at 4 and 7 dpi and categorized into three groups: MHC-Y class I genes, MHC-B region genes, and non-MHC genes. In Eimeria-infected broilers, MHC-Y class I genes were upregulated at 4 dpi but downregulated at 7 dpi. This result implies that MHC-Y class I genes initially activated an immune response, which was then suppressed by Eimeria. Of the MHC-B region genes, the DMB1 gene was upregulated, and TAP-related genes significantly implemented antigen processing for MHC class I at 4 dpi, which was supported by KEGG pathway analysis. Conclusion: This study is the first to investigate MHC gene responses to coccidia infection in chickens using RNA sequencing. MHC-B and MHC-Y genes showed their immune responses in reaction to Eimeria infection. These findings are valuable for understanding chicken MHC gene function.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.15-22
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• 2023

The aim of the present study was to evaluate the chemical composition and antioxidative activity of rhizome essential oil of Kaempferia parviflora Wall. ex Baker. The essential oil extracted by hydrodistillation was chemically profiled by GC/MS analysis. The antioxidative activity was determined and evaluated spectroscopically by the 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays. According to the results, the major essential oil components were camphene (18.03%), β-pinene (14.25%), a-pinene (12.38%), endo-borneol (10.23%), β-copaene (8.38%), and linalool (8.20%). K. parviflora rhizome oil possessed antioxidant potential, exhibiting DPPH and ABTS radical scavenging activities as high as 80.90 and 94.04%, respectively, at a concentration of 10 mg/mL. The corresponding IC₅₀ values were 0.451±0.051 and 0.527±0.022 mg/mL, respectively (IC₅₀ values for ascorbic acid, as the standard, were 0.209±0.016 and 0.245±0.022 mg/mL, respectively). The mycelium of F. oxysporum was distorted and collapsed when treated with 0.5 mg/mL of the EO of K. parviflora rhizome for 3 days treatment, which may provide an important information for exploring the metabolism of the fungicide K. parviflora rhizome and its derived compounds against F. oxysporum. This study provides the chemical properties of the essential oil of K. parviflora rhizome grown in Vietnam and their potential antioxidant and antifungal activities.

• 대한전자공학회논문지TC
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• 제47권1호
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• pp.58-68
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• 2010

본 논문에서는 브리지 기반의 백본망을 위한 프로바이더 백본 브리지(Provider Backbone Bridge) 망에서 각 브리지간 링크장애가 발생한 경우 이를 효율적으로 보호할 수 있는 방법인 백업링크에 대한 스패닝트리 기반의 링크장애복구기능을 제안하고 성능을 분석하였다. 제안된 방식은 PBB with Traffic Engineering (PBB-TE)기반의 브리지망에서 대역예약된 마스터링크의 장애시 해당 링크의 트래픽을 루트브리지가 사전에 설정한 스패닝트리 기반의 백업링크로 우회시킴으로써 해당 트래픽의 QoS를 만족시킨다. 또한 백업링크 상으로 전달되는 프레임들은 스패닝트리 상에서 플러딩되도록 함으로써 주소학습과정에 따른 지연을 최소화시키고 루프백현상도 제거할 수 있는 장점이 있다. 제안된 방식의 동작 검증을 위하여 CCM메시지에 의한 링크보호절체기능과 MAC-in-MAC 수납방법이 모두 지원되는 PBB-TE 시스템을 리눅스 브리지 기반에서 구현하였고 관련 프로토콜 분석도구도 함께 구현하였다. 제안된 방식은 기존의 링크보호방법의 백업링크 예약에 의한 큰 대역손실이나 링크장애시 백업링크를 설정하는데 소요되는 신호절차지연시간이 큰 단점을 해결할 수 있어 QoS를 지원해야 하는 대규모 사업자용 백본 브리지 시스템 구현시 활용될 수 있다.

• 대한한방내과학회지
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• 제32권1호
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• pp.33-42
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• 2011

Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.

• 한국콘텐츠학회논문지
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• 제14권8호
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• pp.74-83
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• 2014

본 연구는 해외 무용예술단체 SNS의 전반적이고 구체적인 분석을 위해 실시되었다. 전 세계 6대륙 100개국의 국 공립, 사설, 개인 무용예술단체 총 3,614개 중 SNS를 1개 이상 운영하는 627개의 단체를 연구대상으로 하였다. 해외 무용예술단체의 6대륙 지역별 분석을 무용장르별, 유형별로 분석 하였으며, 무용장르별 분석을 유형별로 분석 하였다. 또한, 6대륙 지역별, 무용장르별, 유형별로 분석 하여 다음과 같은 결론을 얻을 수 있었다. 첫째, 6대륙 지역에 따른 무용장르별 분석결과 북아메리카 대륙이 SNS를 사용하는 단체가 가장 많은 것으로 나타났다. 둘째, 유형에 따른 6대륙 지역별 분석결과 북아메리카 대륙이 트위터와 페이스북을 가장 많이 사용하는 것으로 나타났다. 셋째, 무용장르에 따른 유형별 분석결과 민속무용단체가 페이스북을 가장 많이 사용 하는 것으로 나타났다. 넷째, 유형에 따른 6대륙 지역별, 무용장르별 분석결과 페이스북, 트위터, 유투브 모두 발레단체가 가장 많이 사용하는 것으로 나타났다. 본 연구 결과를 토대로 SNS 디지털 마케팅에 대한 인식제고, 해외 무용예술단체의 SNS 정보제공, 지식정보체계 구축의 준거로 활용하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권2호
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• pp.189-197
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• 2018

Objective: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. Methods: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. Results: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Conclusion: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9655-9660
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• 2014

Background: Nigella Sativa (NS) is an herb from the Ranunculaceae family that exhibits numerous medicinal properties and has been used as important constituent of many complementary and alternative medicines (CAMs). The ability of NS to kill cancer cells such as PC3, HeLa and hepatoma cells is well established. However, our understanding of the mode of death caused by NS remains nebulous. The objective of this study was to gain further insight into the mode and mechanism of death caused by NS in breast cancer MCF-7 cells. Materials and Methods: Human breast cancer cells (MCF-7) were treated with a methanolic extract of NS, and a dose- and time-dependent study was performed. The $IC_{50}$ was calculated using a Cell Titer $Blue^{(R)}$ viability assay assay, and evidence for DNA fragmentation was obtained by fluorescence microscopy TUNEL assay. Gene expression was also profiled for a number of apoptosis-related genes (Caspase-3, -8, -9 and p53 genes) through qPCR. Results: The $IC_{50}$ of MCF-7 cells was $62.8{\mu}L/mL$. When MCF-7 cells were exposed to $50{\mu}L/mL$ and $100{\mu}L/mL$ NS for 24h, 48h and 72h, microscopic examination (TUNEL assay) revealed a dose- and time-dependent increase in apoptosis. Similarly, the expression of the Caspase-3, -8, -9 and p53 genes increased significantly according to the dose and time. Conclusions: NS induced apoptosis in MCF-7 cells through both the p53 and caspase pathways. NS could potentially represent an alternative source of medicine for breast cancer therapy.