• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.23 no.2
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• pp.125-138
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• 2010

Objective : The present study was examined to evaluate the effects of Ecliptae Herba on the production of inflammatory mediators in vivo and in vitro. Methods : In cell viability, all three doses of Ecliptae Herba extract (25, 50 and $100\;{\mu}g/ml$) had no significant cytotoxicity during the experimental period. The increases of NO production and iNOS expression were detected in LPS-activated cells compared to control, but these increases were dose-dependently attenuated by pre-treatment with Ecliptae Herba extract. Results : 1. LPS plays a pivotal roles in inducing to the massive production of pro-inflammatory cytokines such as TNF-$\alpha$, IL-$1{\beta}$ and IL-6 in macrophages. 2. Ecliptae Herba extract reduced the elevated production of cytokines by LPS. 3. Ecliptae Herba extract reduced $PGE_2$ levels in a dose-dependent manner as a consequence of inhibition of COX-2 activity. 4. Ecliptae Herba extract significantly reduced the nuclear translocation of $NF-{\kappa}B$ induced by LPS. 5. In histopathological study, Ecliptae Herba effectively inhibited the increases of hind paw edema, skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. Conclusions : These results provide evidences that therapeutic effect of Ecliptae Herba on the acute inflammation is partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of $NF-{\kappa}B$.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6433-6438
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• 2013

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

• Journal of Korea Port Economic Association
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• v.26 no.3
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• pp.240-258
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• 2010

The purpose of this paper is to quantify the regional economic effects of the development in Incheon New Port(Stage I) using a regional I/O table and to provide implications for making policies to support the development of the port logistics industry. According to the results of this study, production inducing effect 6,064.5 billion won on Incheon local economy from the development of port facilities at the Incheon New Port (Stage I) occupies 15.0% of 40,398.5 billion won the total production of the Incheon area in 2005, added value inducing effect 2,821.3 billion won occupies 7.7% of 36,508.0 billion won the total added value of the Incheon area in 2005, and induced new employment 72,424 workers occupies 9.0% of 808,248 workers the total number of employees in the Incheon area in 2005. As presented above, the development in the Incheon New Port(Stage I) was expected to have huge spillover effects on Incheon local economy. Accordingly, the development in Incheon New Port must be promoted in a timely manner on national.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.323-332
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• 2019

The purpose of this study is to measure the effects on the regional economy from the Jeju International Wind Ensemble Festival. In order to examine the economic ripple effects of the festival, we examine its impact on the local economy using two regional (Jeju-National) industry-related models based on the 2013 Jeju Region Input and Output Table. We also compare how the Jeju International Wind Ensemble Festival is growing and affecting the regional economy through a comparison between 2017 and 2018. Comparing the results of a production-inducing and value added-effect analysis of the induced industries from investment expenditures for the Jeju International Wind Ensemble Festival, the production-inducing effects increased by 2.1 times-from 9.05 billion won in 2017 to 18.7 billion won in 2018. The value-added effect increased by 2.2 times, from nearly 4.3 billion won in 2017 to nearly 9.2 billion won in 2018. The analysis shows that the Jeju International Wind Ensemble Festival contributes greatly to an income increase for local residents. In order to enhance the effects of the Jeju International Wind Ensemble Festival, it is necessary to use policies that link culture and tourism in Jeju.

• Journal of the Korean Institute of Gas
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• v.20 no.4
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• pp.7-14
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• 2016

The economic crisis in North Korea has reduced its capacity to invest in the energy industries. The country is going through a vicious cycle of decreased investment in the energy industries and reduced energy production. This suggests that the energy industries would come to the top priority of investment once the economy improves. This paper calculated the economic ripple effect of the investment on North and South Korean economies based on the assumption that 390 billion won was invested in the construction of a natural gas combined-cycle power plant in Gaesong Industrial Complex. In order to analyze the economic ripple effect of the investment on North Korean economy, we constructed the inter-industry relation table of North Korea for year 2014 and used the input-output model. The ripple effect of the investment in the natural gas industry turned out to be 1.012 billion dollars. In order to analyze the effect of the investment on South Korean economy, we constructed the inter-industry relation table of South Korea for year 2013 and used the demand-driven model for inter-industry analysis. As a result, production, added-value and employment inducement coefficients of the investment in the natural gas industry were calculated as 2.02073, 0.62697 and 8.99409 respectively.

• IMMUNE NETWORK
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• v.11 no.1
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• pp.79-94
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• 2011

Background: Dendritic cell (DC)-based vaccines are currently being evaluated as a novel strategy for tumor vaccination and immunotherapy. However, inducing long-term regression in established tumor-implanted mice is difficult. Here, we show that deoxypohophyllotoxin (DPT) induces maturation and activation of bone marrow-derived DCs via Toll-like receptor (TLR) 4 activation of MAPK and NF-${\kappa}B$. Methods: The phenotypic and functional maturation of DPT-treated DCs was assessed by flow cytometric analysis and cytokine production, respectively. DPT-treated DCs was also used for mixed leukocyte reaction to evaluate T cell-priming capacity and for tumor regression against melanoma. Results: DPT promoted the activation of $CD8^+$ T cells and the Th1 immune response by inducing IL-12 production in DCs. In a B16F10 melanoma-implanted mouse model, we demonstrated that DPT-treated DCs (DPT-DCs) enhance immune priming and regression of an established tumor in vivo. Furthermore, migration of DPT-DCs to the draining lymph nodes was induced via CCR7 upregulation. Mice that received DPT-DCs displayed enhanced antitumor therapeutic efficacy, which was associated with increased IFN-${\gamma}$ production and induction of cytotoxic T lymphocyte activity. Conclusion: These findings strongly suggest that the adjuvant effect of DPT in DC vaccination is associated with the polarization of T effector cells toward a Th1 phenotype and provides a potential therapeutic antitumor immunity.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.3
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• pp.308-311
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• 2010

An experiment was conducted using indigenous Kivircik ewes to evaluate the effect of intravaginal progestagen sponges, containing 30 mg of fluorogestone acetate (FGA), followed by administration of pregnant mare serum gonadotrophin (PMSG) on inducing synchronized oestrus in the season and fertility. Three times of PMSG administration relative to sponge withdrawal (24 h before (n = 30), at (n = 29) or 24 h after (n= 29)) and two routes of PMSG administration (intramuscular (n = 46) and subcutaneous (n = 42) were compared for estrous response, number of multiple births and fecundity rates. There were no significant differences in terms of estrous response, due to differences in the time and route of PMSG administration. Lambing percentage, proportion of multiple births and fecundity were 75.6, 51.6 and 114.6%, respectively. The administration had a significant effect on lambing (p<0.05), multiple birth and fecundity rates (p<0.01). The subcutaneous administration of PMSG resulted in a significantly higher lambing rate (p<0.05) and fecundity rate (p<0.01), compared to the intramuscular injection of the PMSG.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.2
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• pp.189-194
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• 2016

This paper deals with the economic effects for the Surion(KUH-1) research and development program. The economic effects was measured by Inter-industry analysis method for production-inducing effects, value-added inducement effects, and job-creation effects and by estimating actual domestic input cost. In addition, technical riffle effects was analyzed for aerospace industry, defense industry and commercial industry market through similarity and contribution of technology.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.335-340
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• 2006

A large number of toxicological substances and pharmacological and physical agents can cause reproductive intervention at the cellular and molecular level. The present study was designed to assess the effect of mercury ($HgCl_2$) at 50 to $550{\mu}M$ concentration ranges, in vitro, on the sperm membrane and DNA integrity, viability, and acrosomal status of normal bull spermatozoa. The samples were processed for sperm analyses using semen-diluting fluid (PBS, pH 7.2). We recorded a sharp increase in the lipid peroxidation/LPO rate; the highest was at $550{\mu}M$ mercury concentration, indicating a deleterious effect of mercury on the sperm membrane intactness. There was also a strong negative correlation between LPO rate and % viable spermatozoa (R = 0.987, p<0.001). Data obtained from a comet assay technique revealed that mercury is capable of inducing DNA breaks in the sperm nuclei. Interestingly, 92% of DNA breaks were double-stranded. The correlation between LPO rate and % DNA breaks was 0.984. Performing the gelatin test indicates that mercury is able to alter the integrity of acrosomal membranes showing an abnormal acrosome reaction. In this regard, a strong link was found between LPO rate and % halos (R = 0.990, p<0.001). Collectively, mercury proved to be a potent oxidant in the category of environmental factors affecting bull spermatozoa. Hence, considering the wide spread use of mercury and its compounds, these metals should be regarded with more concern.

• Journal of Ginseng Research
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• v.46 no.1
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• pp.138-146
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• 2022

Background: Red Ginseng has been used for many years to treat diseases. Ginsenoside Rg3 has documented therapeutic effects, including anticancer and anti-inflammatory activities. However, the anticancer effect of Rg3-enriched red ginseng extract (Rg3-RGE) and its underlying mechanisms have not been fully explored. We investigated whether Rg3-RGE plays an anti-tumor role in lung cancer cells. Methods: To examine the effect of Rg3-RGE on lung cancer cells, we performed cell viability assays, flow cytometry, western blotting analysis, and immunofluorescence to monitor specific markers. Results: Rg3-RGE significantly inhibited cell proliferation and induced mitochondria-dependent apoptosis. Furthermore, Rg3-RGE also increased expression of mitophagy-related proteins such as PINK1 and Parkin. In addition, treatment with Rg3-RGE and mitophagy inhibitors stimulated cell death by inducing mitochondria dysfunction. Conclusions: Rg3-RGE could be used as a therapeutic agent against lung cancer.