Browse > Article

Anti-inflammatory effects of the water extract of Ecliptae Herba  

Jo, Hee-Chang (대구한의대학교 안이비인후피부과교실)
Jung, Ho-Jun (대구한의대학교 안이비인후피부과교실)
Kim, Sang-Chan (대구한의대학교 한의과대학)
Jee, Seon-Young (대구한의대학교 안이비인후피부과교실)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.23, no.2, 2010 , pp. 125-138 More about this Journal
Abstract
Objective : The present study was examined to evaluate the effects of Ecliptae Herba on the production of inflammatory mediators in vivo and in vitro. Methods : In cell viability, all three doses of Ecliptae Herba extract (25, 50 and $100\;{\mu}g/ml$) had no significant cytotoxicity during the experimental period. The increases of NO production and iNOS expression were detected in LPS-activated cells compared to control, but these increases were dose-dependently attenuated by pre-treatment with Ecliptae Herba extract. Results : 1. LPS plays a pivotal roles in inducing to the massive production of pro-inflammatory cytokines such as TNF-$\alpha$, IL-$1{\beta}$ and IL-6 in macrophages. 2. Ecliptae Herba extract reduced the elevated production of cytokines by LPS. 3. Ecliptae Herba extract reduced $PGE_2$ levels in a dose-dependent manner as a consequence of inhibition of COX-2 activity. 4. Ecliptae Herba extract significantly reduced the nuclear translocation of $NF-{\kappa}B$ induced by LPS. 5. In histopathological study, Ecliptae Herba effectively inhibited the increases of hind paw edema, skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. Conclusions : These results provide evidences that therapeutic effect of Ecliptae Herba on the acute inflammation is partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of $NF-{\kappa}B$.
Keywords
Ecliptae Herba; Anti-inflammaory effects; NO; $PGE_2$; iNOS; COX-2; $NF{\kappa}B$; TNF-$\alpha$; IL-$1{\beta}$; IL-6;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Babu NP, Pandikumar P, Ignacimuthu S. Anti-inflammatory activity of Albizia lebbeck Benth., an ethnomedicinal plant, in acute and chronic animal models of inflammation. J Ethnopharmacol. 2009;125(2):356-60.   DOI   ScienceOn
2 Holt S, Comelli F, Costa B, Fowler CJ. Inhibitors of fatty acid amide hydrolase reduce carrageenan-induced hind paw inflammation in pentobarbital-treated mice: comparison with indomethacin and possible involvement of cannabinoid receptors. Br J Pharmacol. 2005;146:467-76.   DOI   ScienceOn
3 Kim YW, Zhao RJ, Park SJ, Lee JR, Cho IJ, Yang CH, Kim SG, Kim SC. Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol. 2008;154(1):165-73.   DOI   ScienceOn
4 Delgado AV, McManus AT, Chambers JP. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides. 2003;37(6):355-61.   DOI   ScienceOn
5 Van Q, Nayak BN, Reimer M, Jones PJ, Fulcher RG, Rempel CB. Antiinflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay. J Ethnopharmacol. 2009;125(3):487-93.   DOI   ScienceOn
6 Gurgel AP, da Silva JG, Grangeiro AR, Oliveira DC, Lima CM, da Silva AC, Oliveira RA, Souza IA. In vivo study of the anti-inflammatory and antitumor activities of leaves from Plectranthus amboinicus (Lour.) Spreng (Lamiaceae). J Ethnopharmacol. 2009;125(2):361-3.   DOI   ScienceOn
7 Wu Y, Zhou C, Song L, Li X, Shi S, Mo J, Chen H, Bai H, Wu X, Zhao J, Zhang R, Hao X, Sun H, Zhao Y. Effect of total phenolics from Laggera alata on acute and chronic inflammation models. J Ethnopharmacol. 2006;108(2):243-50.   DOI   ScienceOn
8 Wise LE, Cannavacciulo R, Cravatt BF, Martin BF, Lichtman AH. Evaluation of fatty acid amides in the carrageenaninduced paw edema model. Neuropharmacology. 2008;54(1):181-8.   DOI   ScienceOn
9 Wang T, Fu F, Zhang L, Han B, Zhu M, Zhang X. Effects of escin on acute inflammation and the immune system in mice. Pharmacol Rep. 2009;61(4):697-704.   DOI
10 Tag H, Namsa ND, Das AK, Kalita P, Mandal SC. Evaluation of anti-inflammatory potential of Chloranthus erectus (Buch.-Ham.) Verd. leaf extract in rats. J Ethnopharmacol. 2009;126(2):371-4.   DOI   ScienceOn
11 노민수, 하준용, 이창훈, 이우영, 이수환, 이정준. Inhibitory activities of natural products on lipopolysaccharide induced prostaglandin production in mouse macrophages. 약학회지. 1998;42(6):558-66.
12 서영준. 발암과정에 있어서 cyclooxigenase-2의 역할 및 그 저해를 통한 화학 암예방. 분자세포생물학뉴스. 2001;13:8-17.
13 Cho MK, Suh SH, Kim SG. JunB/AP-1 and $NF-_kB$-mediated induction of nitric oxide synthase by bovine type I collagen in serum stimulated murine macrophages. Nitric Oxide. 2002;6:319-32.   DOI   ScienceOn
14 Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992;6:3051-64.
15 Jirik FR, Podor TJ, Hirano T, Kishimoto T, Loskutoff DJ, Carson DA, Lotz M. Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J Immunol. 1989;142(1):144-7.
16 Chen F, Castranova V, Shi X. New insights into the role of nuclear factor-kappaB in cell growth regulation. Am J Pathol. 2001;159(2):387-97.   DOI   ScienceOn
17 손갑호, 김성훈. 시호(柴胡), 인진(茵蔯)의 간암세포에 대한 항암활성 및 항암제와의 상승(相乘)작용. 대한한의학회지. 1995;16(2):414-32.
18 Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 2009;125(2):286-90.   DOI   ScienceOn
19 Charles A Janeway, Paul Travers, Mark Walport, Mark Shlomchik. Immunobiology. 5th. New York:Garland Publishing. 2001:70-1.
20 Kim HD, Cho HR, Moon SB, Shin HD, Yang KJ, Park BR, Jang HJ, Lim LS, Lee HS, Ku SK. Effect of exopolymers from Aureobasidum pullulans on formalininduced chronic paw inflammation in mice. J Microbiol Biotechnol., 2006;16:1954-60.   과학기술학회마을
21 Rao CV, Verma AR, Gupta PK, Vijayakumar M. Anti-inflammatory and anti-nociceptive activities of Fumaria indica whole plant extract in experimental animals. Acta Pharm. 2007;57(4):491-8.   DOI   ScienceOn
22 Liu J, Zhang W, Zhou L, Wang X, Lian Q. Anti-inflammatory effect and mechanism of osthole in rats. Zhong Yao Cai. 2005;28:1002-6.
23 Cetkovic-Cvrlje M. Eizirik DL. TNF and $IFN\gamma$ potentiate the deleterious effects of $IL-1\beta$ on mouse pancreatic islets mainly via generation of nitric oxide. Cytokine. 1994;6:399-406.   DOI   ScienceOn
24 Beloeil H, Ababneh Z, Chung R, Zurakowski D, Mulkern RV, Berde CB. Effects of bupivacaine and tetrodotoxin on carrageenan-induced hind paw inflammation in rats (Part 1): hyperalgesia, edema, and systemic cytokines. Anesthesiology. 2006;105:128-38.   DOI   ScienceOn
25 Melo PA, Nascimento MC, Mors WB and Suarez-Kurtz G (1994) Inhibition of the mytotoxic and hemorrhagic activities of crotalid venoms by Eclipta prostrata (Asteraceae) extracts and constiuents. Toxicon 32:595-603.   DOI   ScienceOn
26 국윤범. 황련해독탕이 자발적 고혈압 백서의 혈압 및 신장 기능에 미치는 영향. 대한한의방제학회지, 2002;10(1):113-29.   과학기술학회마을
27 Lee JH, Choi YH, Choi BT. The anti-inflammatory effects of 2 Hz electroacupuncture with different intensities on acute carrageenan-induced inflammation in the rat paw. Int J Mol Med. 2005;16:99-102.
28 Gupta M, Mazumder UK, Gomathi P, Selvan VT. Antiinflammatory evaluation of leaves of Plumeria acuminata. BMC Complement Altern Med. 2006;6:36.   DOI
29 Dohi T, Fujihashi K, Rennert PD, Iwatani K, Kiyono H, McGhee JR. Hapten-induced colitis is associated with colonic patch hypertrophy and T helper cell 2-type responses. J Exp Med. 1999;189:1169-80.   DOI   ScienceOn
30 Han. Y, Xia. C et al., Preliminary studies on chemocal constiuents and phamacological action of Eclipta prosrata L. Materia Media. 1983;23:680-2.
31 Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990;144:1425-31.
32 McDaniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. Cytokines and nitric oxides in islet inflammation and diabetes. Proc. Soc. Exp. Biol. Med. 1996;211:24-32.   DOI
33 정영돈, 이현, 이병렬, 임윤경. 족삼리에 시술한 한련초 약침의 면역증진과 항암작용에 관한 연구. 대한침구학회지. 2003;20(3):141-53.
34 Corbett JA, Mac Daniel ML. Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses. J. Exp. Med. 1995;181:559-68.   DOI   ScienceOn
35 윤정식, 정병희, 김나영, 성낙술, 이현용, 이진하, 김종대. 식물자원으로부터 Angiotensin Converting Enzyme 저해활성 탐색. 한국약용작물학회지. 2003;11(3):246-51.   과학기술학회마을
36 천상욱, 김도익, 최용수. 수종의 국화과 식물의 지상부 추출물로부터 살충 및 항균활성 연구. 한국잡초학회지. 2003;23(2):81-91.
37 본초학교수. 본초학. 서울, 영림사. 1992:599-600.
38 이충열, 조선혜, 서종은, 한승호, 조영욱, 민병일, 김창주. 흰쥐 신경세포에서 억제성 및 흥분성 한약재가 유발한 이온전류의 특성. 대한한의학회지. 1998;19(2):450-67.
39 Lee YS, Kim HS, Kim SK, Kim SD. IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans. J Microbiol Biotechnol. 2000;10:8-15.
40 정보섭, 신민교. 도해향약대사전. 서울, 영림사. 2003:1043-4.
41 문영희, 우은란, 이동행. 한련초로부터 정제한 다당체의 혈당강하 효과. 생약학회지. 2003;34(3):246-9.   과학기술학회마을
42 장정애. 한련초 추출물의 모발염색성에 관한 연구. 한국두피모발미용학회지. 2006;2(1):97-109.