• Biomedical Science Letters
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• v.20 no.4
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• pp.250-255
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• 2014

Mycobacterium tuberculosis (MTB) resides and replicates inside macrophages. In our previous report, we reported that CD8+ T cell-mediated immune responses specific for the peptide derived from MTB RNA polymerase beta-subunit ($RpoB_{127-135}$) could be induced in TB patients expressing HLA-$A^*0201$ subtype. In order to examine whether $RpoB_{127-135}$ specific CD8+ T cells can recognize MTB infected macrophages in vitro, CD8+ T cell lines specific for $RpoB_{127-135}$ peptide were generated from peripheral blood mononuclear cells (PBMCs) of healthy HLA-$A^*0201$ subjects by in vitro immunization technique. In this study, we observed $RpoB_{127-135}$ specific CD8+ T cells could recognize and destroy macrophages infected with MTB for 2 to 4 days. $RpoB_{127-135}$ specific CD8+ T cell immune response was inducible from PBMC of healthy subjects expressing HLA-$A^*0206$ subtype, one of HLA-A2 supertype members. Next, we investigated the HLA-I processing mechanism of $RpoB_{127-135}$ peptide in MTB infected macrophages. As a result, the presentation of the MTB derived epitope peptide, $RpoB_{127-135}$, to CD8+ T cells was not inhibited by the treatment with brefeldin-A (ER-Golgi transport inhibitor) or lactacystin (proteasome inhibitor), which blocks the classical HLA-I processing pathway. However, $RpoB_{127-135}$ specific CD8+ T cell activity was blocked either by the blocking agent for the endocytosis (cytochalasin D) or by the blocking antibody (W6/32) for HLA-I molecules. Therefore, the $RpoB_{127-135}$ peptide may be processed by accessing the alternate HLA-I processing pathway. Understanding the processing and presentation mechanisms of the MTB derived proteins will help to improve the efficacy of vaccines and the efficiency of therapeutic agents for TB.

• Journal of Conservation Science
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• v.25 no.4
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• pp.373-381
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• 2009

To preserve steel cultural properties on display outdoors, the surface of these relics is processed with a covering treatment, which is a typical anti-corrosion method. Since the registered cultural heritage of Korea No. 78 Locomotive at Jangdan Station of Gyeongui Line will be treated by preservation process and displayed in the open outdoor area, effective surface covering agents were selected through testing to prevent the corrosion of the locomotive. The mixed wax are based on two types of microcrystalline wax which is one of regular surface covering agents for locomotives and bee‘s wax. For the weather resistance test, 3 types of wax were applied to each specimen of the locomotive and the specimens were tested in the Xenon-Arc Accelerated Weathering Tester for 1000 hours in accordance with the ISO 4892-1981 criteria and the test results were compared. As a result, on the surface of the specimen coated with the bee's wax, a whitening event appears after 400 hours which causes aging, and the specimens coated by the microcrystalline wax showed signs of a whitening event after 800 hours. The moisture contact angle with the microcrystalline wax coating was also less than with the bee's wax. Therefore, it was found that the high temperature microcrystalline wax is the most effective coating agent in terms of weather resistance and moisture blocking capability and the high temperature microcrystalline wax was adopted for coating the surface of the locomotive.

• Imaging Science in Dentistry
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• v.36 no.4
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• pp.189-198
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• 2006

Purpose: To investigate the in vitro response of MC3T3-E1 osteoblastic cells to X-ray in the presence and absence of 2 deoxy-D-glucose (2-DG) and quercetin (QCT). Materials and Methods: The MC3T3-E1 cells were cultured in an ${\alpha}-MEM$ supplemented with 5 mM 2-DG or $10{\mu}M$ QCT and then the cells were incubated for 12 h prior to irradiation with 2, 4, 6, and 8Gy using a linear accelerator (Mevaprimus, Germany) delivered at a rate of 1.5 Gy/min. At various times after the irradiation, the cells were processed for the analyses of proliferation, viability, cytotoxicity, and mineralization. Results: Exposure of the cells to X-ray inhibited the tritium incorporation, 3-(4, 5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT)-reducing activity, and alkaline phosphatase (ALP) activity, and caused cytotoxicity and apoptosis in a dose-dependent manner of the X-ray. This effect was further apparent on day 3 and 7 after the irradiation. RA+2-DG showed the decrease of DNA content, cell viability, and increase of cytotoxicity rather than RA. ALP activity increased on day 7 and subsequently its activity dropped to a lower level. 2-DG suppressed the calcium concentration, but visual difference of number of calcified nodules between RA and RA+2-DG was not noticed. RA+QCT showed the increase of DNA content, cell viability, but decrease of cytotoxicity and subG1 stage cells in the cell cycle, and increased calcified nodules in von Kossa staining rather than the RA. ALP activity showed significant increases on day 7 and subsequently its activity dropped to a lower level. Conclusion: The results showed that the 2-DG acted as a radiosensitizing agent and QCT acted as a radiosensitizing agent respectively in the irradiated MC3T3-E1 osteoblast-like cells.

• Resources Recycling
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• v.25 no.4
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• pp.54-59
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• 2016

EAF processed slag which contains about 20 ~ 35 weight percent FetO is poured to slag pot and cooled. If we recover Fe from molten slag by the reduction, we will improve steel yield rate and reduce slag quantity poured from the furnace. Usually, carbon is used as a reductant and slag foaming agent in the EAF process. In this experiment, after melt the metal in induction furnace and then add slag with carbon and Al dross powder as a reductant, we investigated the reduction of FetO from slag and change of Phophorus content. As the result, when we use Al dross as a reductant, recovery rate is two times more than carbon. Phosphorus pick up is less than 50ppm with reduction of EAF slag.

• YAKHAK HOEJI
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• v.50 no.5
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• pp.332-337
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• 2006

Type 2 diabetes mellitus is a chronic and hard to control disease. In order to develop the therapeutic agent for type 2 DM, many researchers investigated natural products using an in vitro and in vivo assay. In this study, we tried to explore the anti-diabetic activity and mechanisms of ginsam, which is a vinegar-processed ginseng radix. The db/db mice were randomly divided into four groups. The diabetes control (DC) group was orally administrated with distilled water, ginseng radix (GR) or ginsam (GS) was administrated orally at a dose of 150 mg/kg, and the positive control group was orally injected with metformin (MET) at a dose of 300 mg/kg for 5 weeks in db/db mice and measured body weight and blood glucose level every week. All treatment groups decreased the plasma glucose levels compared with diabetic control and GS group significantly lowered the insulin resistance index. GS group also reduced the plasma lipid levels mainly due to reduce the lipogenesis and increase the lipolysis in the fat tissue. In addition, GS group increased the GLUT4 mRNA expression levels in the fat and muscle tissues by 10 fold probably due to increase a $PPAR_{-\gamma}$ mRNA expression in fat tissue. Taken together, GS showed the anti-hyperglycemic and anti-hyperlipidemic activities and those activities may ascribe to over-expression of GLUT4 mRNA level and decrease the lipogenesis in fat tissue.

• The Journal of Korean Academy of Prosthodontics
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• v.31 no.1
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• pp.19-27
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• 1993

For the purpose of this study was to determine the growth of Candide albicans on the surface of the resilient denture liners. The discs$(40\times40mm)$ of 2 resilient lining materals (Molloplast B, Mollosil) and one conventional acrylic resin (K-33) and one metal plate were processed and disinfected. Firstly, the test discs were placed into petri dish, and Candide albicans suspensions was overlayed on the test discs. And the test discs were incubated with intermitant shaking for 1 hour, 2 hours, 6 hours, 12 hours, 24 hours. After incubation, imprint culture method was achived and counted the colony on the agar plate. Secondly, the effect of denture cleansing agents on the growth of Candide alibicans on the resilient dentureliners was evaluated. The results were as follows : 1. The growth of Candida albicans on discs of Molloplast B and Mollosil was increased than that on discs of acrylic resin and metal plate (p<0.05). 2. As Candide albicans suspensions were incubated for 2 hours, the growth of Candida albicans on discs of Mollosil was increased than that on discs of Molloplast B (p<0.05), and the growth of Candide albicans on discs of metal plate was increased than that on discs of acrylic resin (p<0.05). 3. As Candide albicans suspensions were incubated for 6 hours, the growth of Candide albicans on discs of Mollosil was increased than that on discs of Molloplast B (p<0.05). 4. The growth of Candide albicans on discs of Mollosil and Molloplast B in treating denture cleansing agent was inhibited than control discs (p<0.05).

• Applied Microscopy
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• v.32 no.4
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• pp.311-317
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• 2002

There is great requirement on the TEM specimen preparation method with particle size selectivity as a prerequisite for the quantitative structure analysis on the materials such as gibbsite powder, which generally forms a large agglomerate and shows a variation of transition process depending on their sizes. In this experiment, we made an attempt to give a methodology for the TEM specimen preparation of powder with the size selectivity. After mixing 1 wt% gibbsite powder with ethanol solvent, gibbsite suspension was prepared by application of ball-milling and ultrasonification with addition of 0.25 vol% dispersion agent, Darvan C, which was diluted into distilled water by the ratio 1:19. Appling the static sedimentation method to gibbsite suspension after estimation of the sedimentation time by the measurement of accumulative concentration variation, we acquired TEM specimens with well-dispersed and size selected gibbsite particles in nm scale. Overall picture of each sample was taken by SEM and morphology of each dispersed particle was imaged by TEM. Raw and processed gibbsite powders were also examined by XRD to investigate whether they were suffered from phase change during the process or not.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.4
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• pp.1129-1133
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• 2004

For the purpose of developing new anti-HIV agents from natural sources, the extracts of Kalopanax pictus were tested for their inhibitory effects on HIV-1 replication and its essential enzymes as the reverse transcriptase (RT). protease and α-glucosidase. In the assay of HIV-1-infected human T-cell line, water extracts of stem and leafstalk inhibited the HIV-1-induced cytopathic effects with Ie (inhibitory concentration) of 25 and 50㎍/㎖, respectively. Moreover water extracts (100㎍/㎖) of stem and leafstalk showed strong activity of 80% and 90% on anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA) method. In the HIV-1 protease inhibition assay, aqueous stem extract inhibited the activity of the enzyme to cleave an oligopeptide, resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease with 58%, but no glucosidase inhibitory activities. We found out this result, for these samples it is possible that the inhibition of the viral replication in vitro is due to the inhibition at least one of RT and protease. It would be of great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agent up to date are RT, PR and α-glucosidase inhibitors.

• Journal of the Korean Society of Radiology
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• v.9 no.4
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• pp.213-217
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• 2015

To report problems found in a patient who has implemented stent implantation and then conducted a perfusion MRI using ASL(Arterial Spin Labeling), in order to suggest a solution to them. The perfusion MRI was conducted, using pCASL among ASL methods. Data from pCASL(Pseudo Continuous Arterial Spin Labeling) was acquired together with the structural image simply by changing position(labeling gap 15 mm, 170 mm) of the labeling pulse to avoid stent. Data was processed through the ASLtbx. When perfusion MRI was acquired using pCASL, it showed that the position of the conventional labeling pulse (labeling gap 24 mm) was overlapped with that of stent, which made signal intensity in right brain tissue appear as if it were void. When the labeling pulse was positioned (labeling gap 15 mm) to avoid stent, high signal intensity images were acquired. In labeling pulse (labeling gap 170 mm), the signal intensity was more reduced due to relaxation before labeled blood arrived at the imaging slice. pCASL can be stably repeated measurements because it does not use a contrast agent. And it should be selected with the appropriate image acquisition parameters for the high quality image.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.115-122
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• 1997

Grafting is an important skill for the stable supply and production of high quality. However, the shortage of skillful labor has become great difficulty for a mass production of grafting-seedling. In this study, a suitable mechanism for a grafting machine was developed. The following summarize the results of this study: 1. An insertion method was selected for mechanism of the grafting machine without bonding agent, clip, pin. This insertion-grafting method can be applicable to general vegetables and a mass production system. In addition to, this method is suitable for developing the grafting mechanism. 2. Growing point was removed while remaining both cotyledons on rootstock. The productivity of this system was five fold greater than the one of an experienced labor. 3. The rootstock processing was placed on left and scion processing unit was placed on right of the system, then processed rootstock and scion graft by rotating $180^{\circ}$. 4. The efficiency tests on mechanical grafting rate showed 98%.