• Title/Summary/Keyword: probe

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A Study on the measurement of Electron Energy Distribution Function in Ar plasma measured by the waveforms of Langmuir probe voltages (Langmuir 프로브 전압의 파형에 따른 아르곤 플라즈마의 전자에너지 분포함수 측정에 관한 연구)

  • Kim, Du-Hwan;Park, Jeong-Hu
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.48 no.5
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    • pp.391-395
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    • 1999
  • In this paper, we have obtained the Electron Energy Distribution Function(EEDF) in plasma by using two differentiators and investigated the EEDFs by sawtooth and triangle waveform voltages with the working pressures and the positions of single probe. It is found that as the working pressure is decreased, the EEDFs approach to theMaxwellian distribution independent of the waveforms of probe voltage. On the otherhand, as the position of probe is moved from the center of the plasma to its edge, the EEDF of sawtooth waveform probe voltage approaches to the Maxwellian distribution, but the EEDF of triangle waveform probe voltage deviates from the Maxwellian distribution.

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Development of a Laboratory-based Calibration System for 5-Hole Probes (5공 프로브 실험실용 교정 시스템 개발)

  • Kim, Changmin;Baek, Seungchan;Ji, Changeun;Hwang, Wontae
    • Journal of the Korean Society of Visualization
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    • v.18 no.3
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    • pp.122-128
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    • 2020
  • In the field of experimental fluid dynamics, the 5-hole probe is one of the most widely used tools to measure flow velocity and pressure. We hereby describe the development of an inexpensive laboratory-based flow calibration system for 5-hole probes. The system is applied to a custom L-shaped probe, and the probe performance is compared against a standard commercial probe in a custom wind tunnel. The setup allows rotation of the probe around the yaw and pitch axes. Static and total pressure values are calculated, and then calibration maps are constructed based on the yaw and pitch angles. Using these maps, errors of the custom probe are found to be ±5% for velocity magnitude and ±3° for direction, compared to the commercial probe, when both pitch and yaw angles are within 40°.

Measurement and Analysis of Ground Impedance according to Arrangement of Auxiliary Probe around Ground Grid (접지 그리드에서의 보조전극 배치에 따른 접지임피던스 측정 및 분석)

  • Gil, Hyoung-Jun;Shong, Kil-Mok;Kim, Young-Seok;Kim, Chong-Min
    • Journal of the Korean Society of Safety
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    • v.30 no.4
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    • pp.46-50
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    • 2015
  • This paper describes the measurement and analysis of ground impedance according to arrangement of auxiliary probe around ground grid using the fall-of-potential method and the testing techniques to minimize the measuring errors are proposed. The fall-of-potential method involves passing a current between a ground electrode and a current probe, and then measuring the voltage between a ground electrode and a potential probe. To minimize interelectrode influences due to mutual resistances, the current probe is a generally placed at a substantial distance from the ground electrode under test. In order to analyze the effects of ground impedance due to the arrangement of auxiliary probe and frequency, ground impedances were measured in case that the arrangements of auxiliary probe were straight line, perpendicular line, and horizontal line. The distance of current probe was located from 10[m] to 200[m] and the measuring frequency was ranged from 55[Hz] to 513[Hz]. As a consequence, the ground impedance increases with increasing the distance from the ground electrode to the point to be tested, but the ground impedance decreases with increasing the frequency.

Characteristics of MEMS Probe Tip with Multi-Rhodium Layer (이중 로듐 층을 갖는 멤스 프로브 팁의 특성)

  • Park, Dong-Gun;Park, Yong-Joon;Lim, Seul-Ki;Kim, Il;Shin, Sang-Hun;Cho, Hyun-Chul;Park, Seung-Pil;Kim, Dong-Won
    • Journal of the Korean institute of surface engineering
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    • v.45 no.2
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    • pp.81-88
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    • 2012
  • Probe tip, which should have not only superior electrical characteristics but also good abrasion resistance for numerous contacts with semiconductor pads to confirm their availability, is essential for MEMS probe card. To obtain good durability of probe tip, it needs thick and crack-free rhodium layer on the tip. However, when the rhodium thickness deposited by electroplating increased, unwanted cracks by high internal stress led to serious problem of MEMS probe tip. This article reported the method of thick Rh deposition with Au buffer layer on the probe tip to overcome the problem of high internal stress and studied mechanical and electrical properties of that. MEMS probe tip with double-Rh layer had good contact resistance and durability during long term touch downs.

An improvement of real-time polymerase chain reaction system based on probe modification is required for accurate detection of African swine fever virus in clinical samples in Vietnam

  • Tran, Ha Thi Thanh;Dang, Anh Kieu;Ly, Duc Viet;Vu, Hao Thi;Hoang, Tuan Van;Nguyen, Chinh Thi;Chu, Nhu Thi;Nguyen, Vinh The;Nguyen, Huyen Thi;Truong, Anh Duc;Pham, Ngoc Thi;Dang, Hoang Vu
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.10
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    • pp.1683-1690
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    • 2020
  • Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including realtime polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.

Design of Vertical Type MEMS Probe with Branch Springs (분기된 구조를 갖는 수직형 MEMS 프로브의 설계)

  • Ha, Jung-Rae;Kim, Jong-Min;Kim, Byung-Ki;Lee, June-Sang;Bae, Hyeon-Ju;Kim, Jung-Yup;Lee, Hak-Joo;Nah, Wan-Soo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.7
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    • pp.831-841
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    • 2010
  • The conventional vertical probe has the thin and long signal path that makes transfer characteristic of probe worse because of the S-shaped structure. So we propose the new vertical probe structure that has branch springs in the S-shaped probe. It makes closed loop when the probe mechanically connects to the electrode on a wafer. We fabricated the proposed vertical probe and measured the transfer characteristic and mechanical properties. Compared to the conventional S-shaped vertical probe, the proposed probe has the overdrive that is 1.2 times larger and the contact force that is 2.5 times larger. And we got the improved transfer characteristic by 1.4 dB in $0{\sim}10$ GHz. Also we developed the simulation model of the probe card by using full-wave simulator and the simulation result is correlated with measurement one. As a result of this simulation model, the cantilever probe and PCB have the worst transfer characteristic in the probe card.

Application of DNA Probe Method for Detection of 2,4-Dichlorophenoxyacetic Acid Degrading Bacteria in Soil (DNA Probes에 의한 토양의 이사디 (2,4-D) 분해세균의 검출)

  • Ka, Jong-Ok
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.403-408
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    • 1996
  • Total bacterial community DNA, which was extracted from microcosm soil and field soil after 2,4-D amendments, was analyzed on Southern blots, using the tfdA gene probe derived from plasmid pJP4 and the Spa probe from Sphingomonas paucimobilis. Southern blot analyses with total bacterial DNA extracted from soils Inoculated with Pseudomonas cepacia/pJP4 revealed that DNA probe method could detect the 2,4-D degrading bacteria down to $10^5\;cells/g$ dry soil. In the microcosm experiment, there was a good correlation between 2,4-D degradation and banding patterns in hybridization analyses performed after each 2,4-D treatment using the two probes. When bacterial DNA extracted from microcosm soil was hybridized with the Spa probe, a change in the position of hybrid bands was observed over time in a Southern blot, suggesting that population change or possibly genetic rearrangement in 2,4-D degrading microbial populations occurred in this soil. With the Spa probe, one hybrid DNA band was persistently observed throughout the five 2,4-D additions. When bacterial DNA isolated from the field soil was probed with the tfdA and Spa, strong hybridization signal was observed in the 100 ppm-treated subplot, weak signal In the 10 ppm-treated subplot, and no significant signal in the 1 ppm-treated and control subplots. The data show that DNA probe analyses were capable of detecting and discriminating the indigenous 2,4-D degrading microbial populations in soil amended with 2,4-D under laboratory and field conditions.

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Design and Fabrication of a 3-dimensional Diagnostic Ultrasonic Probe (3차원 입체 영상 진단용 초음파 프로브의 설계 및 제작)

  • Eun, Hong;Lee, Su-Sung;Roh, Yong-Rae
    • The Journal of the Acoustical Society of Korea
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    • v.21 no.8
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    • pp.766-771
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    • 2002
  • In this study, we have developed a 3-dimensional diagnostic ultrasonic sector probe using a convex type ultrasonic probe with 128 active elements. The probe was made to operate at the center frequency of 4.5㎒ with the bandwidth of 66%. The driving part was designed to rotate the axis of the convex probe by means of a step motor equipped with reduction gears and spur gears attached to the motor so that the probe could enable us to acquire a series of 2-dimensional images to construct a 3-dimensional image. Acoustic cover of the probe was made of polymers to have the same radius of rotation as that of the convex probe. The controllability of the rotation angle and the structural stability of the probe were confirmed through experimental 3-dimensional images obtained using the developed 3-dimensional sector probe.

Assessment of Design and Mechanical Characteristics of MEMS Probe Tip with Fine Pitch (미세 피치를 갖는 MEMS 프로브 팁의 설계 및 기계적 특성 평가)

  • Ha, Seok-Jae;Kim, Dong-Woo;Shin, Bong-Cheol;Cho, Myeong-Woo;Han, Chung-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.4
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    • pp.1210-1215
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    • 2010
  • The probe card are test modules which are to classify the good semiconductor chips and thin film before the packaging process. In the rapid growth a technology of semiconductor, the number of pads per unit area is increasing and pad arrays are becoming irregular. Therefore, the technology of probe card needs narrow width and lots of probe tip. In this paper, the probe tip based on the MEMS(Micro Electro Mechanical System)technology was developed a new MEMS probe tip for vertical probe card applications. For the structural designs of probe tip were performed to mechanical characteristics and structural analysis using FEM(Finite Element Method). Also, the contact force of MEMS probe tip compared with FEM results and experimental results. Finally, the MEMS probe card was developed a fine pitch smaller than $50{\mu}m$.

Drug-Biomacromolecule Interaction VII

  • Kim, Chong-Kook;Yang, Ji-Sum;Lim, Yun-Su
    • Archives of Pharmacal Research
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    • v.7 no.1
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    • pp.11-15
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    • 1984
  • Binding of sic cephalosporins (cefotaxime, cefuroxime, cafazoline, cephalothin, cephaloridine, cephacetrile) to human serum albumin was studied. Fluorescence probe technique and difference spectrophotometry were employed to evaluate the nature and degree of association of cephalosporin-albumin complex. 1-anilinonaphthalene-8-surfonate was used as the fluorescence probe, and 2-(4'-hydroxybenzeneazo)benzoic acid as the UV spectrophotometric probe. Competitive bindings between cephalosporins and probe were observed. For the binding of cephalosporins to human serum albumin, three binding sites were identified by fluorescence probe technique but four binding constants of cephalosporins to human serum albumin measured by fluorescence probe technique are higher than those meausred by UV spectrophotometry.

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