• Proceedings of the Korean Society of Life Science Conference
• /
• 2001.09a
• /
• pp.46-46
• /
• 2001

• Korean Journal of Adult Nursing
• /
• v.24 no.1
• /
• pp.64-73
• /
• 2012

Purpose: The purpose of this study was to investigate emergency nurses' reported traumatic events, professional quality of life and physical symptoms. Methods: The design of the study was descriptive. The respondents were 157 Emergency nurses with the majority were women (94.3% female and 5.7% were men). Data were collected from September 28 to December 1, 2011. The professional quality of life (ProQOL) instrument is conceptualized with three sub-dimensions (compassion satisfaction, burnout, and secondary traumatic stress), in addition information about traumatic events, physical symptoms and demographic variables were collected. Burnout and secondary traumatic stress (STS) are two parts of compassion fatigue. Data were analyzed using the SPSS/WIN 18.0 program and included descriptive statistics, t-test, ANOVA, Pearson's correlations. Results: Thirteen percent of the participants reported a high ProQOL. Compassion satisfaction was negatively correlated with burnout and physical symptoms, whereas, traumatic events had positive correlations with STS. Also, burnout and STS were positively correlated with physical symptoms respectively. Conclusion: Since the level of professional quality of life among emergency nurses was relatively low it is recommended that an intervention program for emergency nurses be instituted to improve compassion satisfaction and to decrease compassion fatigue and physical symptoms.

• BMB Reports
• /
• v.38 no.3
• /
• pp.290-293
• /
• 2005

In order to investigate the epitope of basic fibroblast growth factor (bFGF) and its immunogenicity, the epitopes of bFGF were screened from the phage display library with monoclonal antibody GF22, which can neutralize the bio-activity of bFGF. By three rounds of screening, the positive phage clones with bFGF epitopes were selected, which can effectively block the bFGF to bind with GF22. Sequence analysis showed that the epitopes shared a highly conservative sequence (Leu-Pro-Pro/Leu-Gly-His-Phe/Ile-Lys). The sequence of PPGHFK was located at 22-27 of the bFGF. The specific immuno-response of mouse could be highly induced by phage clones with the epitopes. And the anti-bFGF activity induced by LPGHFK was 3 times higher than the original sequence, which showed that the mimetic peptide LPLGHIK might be used as a tumor vaccine in the prevention and treatment of tumor.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.22 no.2
• /
• pp.83-93
• /
• 2011

Biochemical and enzymatic characterization for extracellular protease isolated from Cordyceps militaris cultivated on rice bran medium was investigated. C militaris produced proteolytic enzymes from 10 days after inoculation, maximum enzyme production was found at 25 days. The optimum temperature and pH of proteases production was at $25^{\circ}C$ and pH 7.0, respectively. The protease activity was observed in the four peaks (Pro-I, Pro-II, Pro-III, and Pro-IV) separated through Sephadex G-100 column chromatography. The separated protease was optimally active at $25^{\circ}C$. Optimum pH of the protease was between 7 and 8. Enzyme was also stable over at $30-80^{\circ}C$. The enzyme was highly stable in a pH range of 4-9. Protease activity was found to be slightly decreased by the addition of $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, $Fe^{2+}$ and $Cu^{2+}$, whereas inhibited by the addition of $Ca^{2+}$ and $Co^{2+}$ Protease activity was inhibited by protease inhibitor PMSF. On the other hand, the partially purified protease was investigated on proteolytic protease activity by zymogram gel electrophoresis using three substances (casein, gelatin and fibrin). Four active bands (F-I, FII, F-III, and F-IV) of fibrin degradation were revealed on fibrin zymogram gels. Both of F-II and FIII showed caseinolytic, fibrinolytic and gelatinolytic activities in three gels. Thermostability, pH stability, and pH-thermostability of the enzyme determined the residual fibrinolytic activity also displayed on fibrin zymogram gel. The only one enzyme (F-II) displayed over a broad range of temperature at $30-90^{\circ}C$. The FII displayed fibrinolytic activity in the pH range 3-5, but was inactivated in the range of pH 6-11. The F-I and F-III showed enzyme activity in the pH range of 6-11. In the pH-thermostability, the F-II only kept fibrinolytic activity after heating at $100^{\circ}C$ for 10, 20 and 30 min at pH 3 and pH 7, respectively. On the other hand, the F-II was retained activity until heating for 10 min under pH 11 condition. By using fibrin zymogram gel electrophoresis, extracellular fibrinolytic enzyme F-II from C. militaris showed unusual thermostable under acid and neutral conditions.

• Korean Journal of Human Ecology
• /
• v.16 no.5
• /
• pp.957-967
• /
• 2007

The purposes of this study were to find the sub-factors of consumer consciousness toward well-being and to identify the variables influencing their well-being oriented consumer behaviors in the area of dietary life of married women. 579 married women were surveyed by internet portal site azoomma.com. The collected data were treated with the SPSS Windows 12.0 program and analyzed in terms of frequency, percentage, mean, standard deviation, oneway ANOVA, factor analysis, and multiple regression analysis. The findings were summarized as follow: 1. Consumer consciousness toward well-being has four sub-factors; pro-environmental attitude, personal and family health, means of business, and consciousness regarding others. 2. In the multiple regression analysis, concerns of information, price level, consciousness of personal and family health, health status, educational level explained the purchasing behavior about 25.7%. On the other hand, health status, pro-environmental attitude, consciousness of personal and family health, consciousness regarding others, and concerns of information explanined the using behaviors about 17.0%. Pro-environmental attitude, health status, consciousness of personal and family health, consciousness regarding others, and status of employment were effective variables, and explained the disposal behavior about 19.5%.

• Proceedings of the Korean Society of Life Science Conference
• /
• 2006.09a
• /
• pp.55.1-55.1
• /
• 2006

• Korean Journal of Remote Sensing
• /
• v.15 no.4
• /
• pp.339-348
• /
• 1999

To extract value-added products which are important in scientific area and practical life, e.g. digital elevation models, ortho-rectified images and geometric corrected images, Satellite Technology Research Center at Korea Advanced Institute of Science and Technology has developed a satellite image processing software called "Valadd-Pro". In this paper, "Valadd-Pro" software is briefly introduced and its main components such as precise geometric correction, ortho-rectification and digital elevation model extraction component are described. The performance of "Valadd-Pro" software was assessed on 10m resolution 6000 $\times$ 6000 SPOT panchromatic images (60km $\times$ 60km) using ground control points from GPS measurements. The height accuracy was measured by comparing our results with 100m resolution $DTEDs^{1)}$ produced by USGS and 60m resolution DEMs generated from digitized contours produced by National Geography Institute. Also, to show the superior performance of "Valadd-Pro" software, we compared the performance with that of commonly used PCI$\circledR$ commercial software. Based on the results, the geometric correction of "Valadd-Pro" software needs fewer ground control points than that of PCI$\circledR$ software and the ortho-rectification of "Valadd-Pro" software shows similar performance to that of PCI$\circledR$ software. In the digital elevation model extraction, "Valadd-Pro" software is two times more accurate and four times faster than PCI$\circledR$ software.ccurate and four times faster than PCI$\circledR$ software.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.7
• /
• pp.1027-1032
• /
• 2008

A study was conducted to evaluate the effects of dietary yeast derived ${\beta}$-glucan and single-strain probiotics on the growth performance and antibody response in broiler chicks. Six hundred and thirty 1-d-old male broiler chicks were divided into seven groups, placed into three pens per group (30 birds per pen) and fed one of seven non-medicated corn-SBM based experimental diets containing 0.025, 0.05 or 0.1% Saccharomyces cerevisiae ${\beta}$-glucan and 0.05, 0.1 or 0.2% Bacillus amyloliquefaciens (BA-pro, $1.3{\times}10^9/g$) or devoid of them for 5 wk. The body weight gains in groups fed diets containing 0.025 or 0.1% ${\beta}$-glucan, 0.1% or 0.2% BA-pro were significantly higher (p<0.05) than the control over 1-35 d. Feed conversion rates of groups fed ${\beta}$-glucan and BA-pro tended to be improved compared to the control group. There were no significant differences in the relative weights of liver, abdominal fat and breast muscle. No significant differences were observed in the activities of serum enzymes and concentrations of various cholesterol fractions. The antibody titers against Newcastle disease or infectious bronchitis virus in the chicks fed diets containing ${\beta}$-glucan and BA-pro were significantly higher (p<0.05) than in the control. The concentrations of cecal lactic acid bacteria in all groups fed BA-pro were significantly increased (p<0.05) compared to the control. These results indicated that dietary yeast derived ${\beta}$-glucan and BA-pro exerted growth-promoting and immune-enhancing effects in broiler chickens. In addition, BA-pro added to the diets modulated the profiles of cecal microflora, reflecting a potential to be beneficial microorganisms in chickens.

• Journal of Life Science
• /
• v.11 no.1
• /
• pp.42-46
• /
• 2001

Subtilisin YaB, produced by alkalophilic Bacillus strain YaB, is an extracellular alkaline serine protease having 55% homology to subtilisin BPN'. It is synthesized as a 378-amino acid preproenzyme and secreted into the culture medium as a 265-amino acid mature protease. To examine the role of pro-sequence for the secretion of subtilisin YaB, we have studied the expression, in Bacillus subtilis, of a mutant preprosubtilisin YaB in which active site Ser214 is substituted with Cys. The use of a six protease-deficient strain, WB600, was required for its efficient production. The prosubtilisin YaB, thus produced, was indeed secreted into the culture medium and was processed to its mature form upon treatment with exogenously added active subtilisin YaB. From these results, we have concluded that the processing of pro-sequence is not essential for the secretion of the enzyme.

• Journal of Life Science
• /
• v.10 no.2
• /
• pp.140-149
• /
• 2000

In order to utilize marine processing waste which would normally be discarded, cod liver protein was hydrolysed by ${\alpha}$-chymotrysin, and the hydrolysate was investigated for the new angiotensin I converting enzyme (ACE) inhibitor. Thy hydrolysate was separated into three major types, with molecular weight cut-off (MWCO) values less than 10 kDa, 5 kDa and 1 kDa of ultrafiltration membranes, respectively. ACE inhibitory peptides were isolated from the fractions passed through MWCO 1 kDa membrane, and purified by using ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, and HPLC on an ODS column. The purity was identified with capillary electrophoresis. The amino acid sequences of two peptides were Met-Ile-Pro-Pro-Tyr-Tyr (IC50=10.9 ${\mu}$M) and Gly-Leu-Arg-Asn-Gly-Ile (IC50=35.0 ${\mu}$M)