• Journal of Korean Academy of Oral Health
• /
• 제42권4호
• /
• pp.224-228
• /
• 2018

Objectives: As a first step to study the anticaries effect of ethanol alone, we investigated the effects of ethanol on the expression levels of the atpB gene and proton permeability of Streptococcus mutans in suspension cultures. Methods: S. mutans UA159 was grown in brain heart infusion medium at either pH 4.8 or 6.8. The total extracted RNA was reverse-transcribed into cDNA using a $Superscript^{TM}$ First-Strand Synthesis System. The resulting cDNA and negative controls were amplified by ABI PRISM 7700 real-time PCR system with SYBR Green PCR Master Mix. For proton flux assay, bacterial suspensions were titrated to pH 4.6 with 0.5 M HCl, and then additional 0.5 M HCl was added to decrease the pH values by approximately 0.4 units. The subsequent increase in pH was monitored using a glass electrode. Ten percent (v/v) butanol was added to the suspensions at 80 min to disrupt the cell membrane. Results: In a concentration-dependent manner, ethanol alone not only decreased the growth rate of S. mutans and the expression of the atpB gene but also increased the proton permeability at both pH 4.8 and 6.8. Conclusions: These findings suggest that ethanol has the potential for an anticaries ingredient. We believe that ethanol may be used together with fluoride and/or other cariostatic agents in order to develop better anticaries toothpastes and/or mouthrinses.

• 한국지형학회지
• /
• 제19권3호
• /
• pp.153-163
• /
• 2012

지구 지표는 암석권 내 혹은 암석권 하부의 다양한 동적 원인에 의해 수직적으로 변형될 수 있으나, 판 내부 지역에서 발생하는 다양한 수직적 지각 변형의 원인과 매커니즘을 구분해내는 일은 여전히 지구과학 분야에 있어 기초적인 도전의 영역으로 남아있다. 이러한 과학적 도전에 있어 가장 중요한 전제 조건은 다양한 지구조 환경에서의 보다 많은 대륙 지각 변형 사건들을 파악하고 시 공간적 패턴을 구분해보는 일이 될 것이다. 본 연구는 최근의 연구 성과들에 바탕하여 한반도의 신생대 신제3기 이후 융기 운동을 포함한 판 내부 대륙 지각에서 발생하는 다양한 수직적 변형 사건들의 다양한 지구역학적 요인들을 설명하고자 하는데 그 목적을 두고 있다. 궁극적으로 지구지표 형태와 지구 내부 활동간의 상호작용에 대한 이해는 지구 내부 활동의 다양한 신호들을 보여주는 이상적인 프리즘으로서 지표 지형의 중요성을 보여 줄 것으로 확신한다.

• 지식경영연구
• /
• 제21권4호
• /
• pp.243-263
• /
• 2020

본 연구는 공공데이터 개방에 있어 공공데이터 제공자의 데이터 기여 측면과 공공데이터 사용자의 데이터 활용 측면을 고려하여 공공데이터 플랫폼 성과측정을 위한 프레임워크를 개발하였다. 본 연구는 NIST(2018)의 빅데이터 참조 아키텍처와 Neely et al.(2001)의 성과 프리즘을 기반으로 공공 빅데이터 플랫폼 성과평가 모형의 5개 영역을 제시하였다. 구체적으로, 공공데이터 플랫폼 성과평가 영역은 이해관계자 기여, 빅데이터 거버넌스 역량, 빅데이터 서비스 역량, 빅데이터 정보기술(IT) 역량, 그리고 이해관계자 만족으로 구성된다. 본 연구에서 제시한 공공 빅데이터 플랫폼 성과평가 모형의 5개 영역과 24개 평가지표에 대한 측정 문항은 총 75개 항목으로 구성되었다.

• Genomics & Informatics
• /
• 제20권3호
• /
• pp.30.1-30.9
• /
• 2022

Hereditary spastic paraplegia is a not common inherited neurological disorder with heterogeneous clinical expressions. ALDH18A1 (located on 10q24.1) gene-related spastic paraplegias (SPG9A and SPG9B) are rare metabolic disorders caused by dominant and recessive mutations that have been found recently. Autosomal recessive hereditary spastic paraplegia is a common and clinical type of familial spastic paraplegia linked to the SPG11 locus (locates on 15q21.1). There are different symptoms of spastic paraplegia, such as muscle atrophy, moderate mental retardation, short stature, balance problem, and lower limb weakness. Our first proband involves a 45 years old man and our second proband involves a 20 years old woman both are affected by spastic paraplegia disease. Genomic DNA was extracted from the peripheral blood of the patients, their parents, and their siblings using a filter-based methodology and quantified and used for molecular analysis and sequencing. Sequencing libraries were generated using Agilent SureSelect Human All ExonV7 kit, and the qualified libraries are fed into NovaSeq 6000 Illumina sequencers. Sanger sequencing was performed by an ABI prism 3730 sequencer. Here, for the first time, we report two cases, the first one which contains likely pathogenic NM_002860: c.475C>T: p.R159X mutation of the ALDH18A1 and the second one has likely pathogenic NM_001160227.2: c.5454dupA: p.Glu1819Argfs Ter11 mutation of the SPG11 gene and also was identified by the whole-exome sequencing and confirmed by Sanger sequencing. Our aim with this study was to confirm that these two novel variants are direct causes of spastic paraplegia.

• International Journal of Computer Science & Network Security
• /
• 제22권10호
• /
• pp.291-297
• /
• 2022

Modern challenges in the educational environment force scientists and practitioners to search for an adequate answer. In particular, the war in Ukraine demonstrated the importance of developing information competence as one of the main means of distinguishing true information from a whole stream of fake news. This is especially relevant in connection with the introduction of distance learning when students must find and process a large amount of information on their own. Therefore, the purpose of the article is to analyze the training of higher education students through the prism of acquiring the necessary level of informational competence in war conditions. For this, general scientific and special research methods, as well as the experimental method, were used. In the results, the peculiarities of the interpretation of information competence in the distance form of education among modern researchers are determined, the psychological components of resistance to fakes are analyzed. Based on the conducted empirical measurements, it was established that thorough work on student education gives positive skills when working independently with Internet materials, strengthens the ability to distinguish false information and propaganda from the real state of affairs. The conclusions summarize the results of the empirical research and suggest ways to improve the situation with the formation of information competence.

• Archives of Plastic Surgery
• /
• 제49권3호
• /
• pp.462-470
• /
• 2022

Background Reactive oxygen species cause serious damage to the physiological function of tissues. Determination of total antioxidant capacity of skin tissue is one of the determinants of damaged tissue function. Mast cells (MCs) are one of the groups of cells that are invited to the site of injury. The healing process begins with the rapid release of various types of MCs' intermediate factors at the site of injury. Bone marrow mesenchymal stem cell (BMMSC) production and secretion have been shown to regenerate the skin. The aim of this research was to evaluate the wound-healing and antioxidant effects of BMMSCs per MCs. Methods Fifty-four albino Wistar male rats were divided into three groups: (1) nonsurgery, (2) surgery, and (3) surgery + BMMSCs. Groups 2 and 3 were operated with a 3 × 8 cm flap and in group 3, cell injections (7 × 10⁹ cell injection at the time of surgery) were performed. After days 4, 7, and 15, percentage of the surviving tissue, histological characteristics, superoxide dismutase (SOD) activity, and amount of malondialdehyde (MDA) were measured in the groups. For results, Graph Pad Prism 8 software was used, and data were analyzed and compared by analysis of variance and Tukey test. Results BMMSCs' application decreased the amount of MDA, increased SOD activity and survival rate of the flaps, and improved the histological characteristics. Conclusion This study revealed the protective effects BMMSCs alongside MCs against oxidative stress on the survival of the flaps. However, for clinical use, more research is needed to determine its benefits.

• International Journal of Computer Science & Network Security
• /
• 제22권10호
• /
• pp.165-170
• /
• 2022

Nowadays more and more attention is paid to digital technologies, digitization, and the digitization of public services in particular. Progressive countries aim to build "digital" governance and a digital economy. In this aspect, the international experience of using digitization in the field of public administration, including in local self-government bodies, plays an important role. Therefore, it is important to analyze the international legal experience of the functioning of local self-government in the conditions of digitization. The purpose of the work is to study the international legal experience of the functioning of local self-government in the conditions of digitization. The object of the study is local self-government in the conditions of digitization through the prism of international legal experience. The subject of the study is social relations that arise, change, and cease during the implementation of digitization in local self-government in Ukraine and foreign countries. The research methodology consists of such methods as the method of philosophical hermeneutics; dialectical method; classification method; comparative method; target method; method of documentary analysis; generalization method. Research results. As a result of the conducted research, the international experience of the functioning of local self-government in conditions of digitization was analyzed. In particular, the impact of digitization processes on the course of the decentralization reform in Ukraine is summarized. Also, from the analysis of international experience, a conclusion was made about the potential possibility of reducing the existing gap in the process of communication between the central government and united territorial communities thanks to the introduction of the latest technologies in the field of administrative services, to stimulate innovative and economic development of regions, attracting the attention of businesses and potential investors, as well as the functioning of more open local authorities with electronic communication tools.

• International Journal of Computer Science & Network Security
• /
• 제23권2호
• /
• pp.1-12
• /
• 2023

The article examines the axiological psycho-philosophical understanding of the phenomenon of money and its value role in modern society. The traditional and virtual context of the representation of the money phenomenon is considered.Following the ideas of G. Simmel, the authors consider money not only as a purely economic, but also a psycho-philosophical, cultural and social phenomenon. Money appears as a result of cultural development of the world and gradually forms a monetary culture as a space of economic and social interaction of people. Under the influence of the monetary culture of one or another historical period, the character of a person's economic activity, values and life orientations are formed. Modern money culture is often called financial civilization. Peculiarities of modern monetary culture are studied, its main features and problems are determined in the article. The problem of the peculiarities of the constructive and destructive attitude of the individual towards money is identified; a psycho-philosophical and cultural-identification typology of people is described, which is based on clinical observations and interpreted through the prism of psychoanalytic theory. The concept of money is highlighted from the standpoint of a social-psychological approach. The theoretical foundations of money's influence on the decision-making process and human behavior are also revealed.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2023년도 학술발표회
• /
• pp.519-519
• /
• 2023

Estuarine dams are a recent and global phenomenon. While estuarine dams can provide the benefit of improved freshwater resources, they can also alter estuarine processes. Due to the wide range of estuarine types and estuarine dam configurations, the effect of estuarine dams on estuaries is not well understood in general. To develop a systematic understanding of the effect of estuarine dam location and freshwater discharge interval on a range of estuarine types (strongly stratified, partially mixed, periodically stratified, and well-mixed), this study used a coupled hydrodynamic-sediment dynamic numerical model (COAWST) and compared flow, sediment transport, and morphological conditions in the pre- and post-dam estuaries. For each estuarine type, scenarios with dam locations at 20, 55 and 90 km from the mouth and discharge intervals of a discharge every 0.5, 3, and 7 days were investigated. The results were analyzed in terms of change in tide, river discharge, estuarine classification, and sediment flux mechanism. The estuarine dam location primarily affected the tide-dominated estuaries, and the resonance length was an important length scale affecting the tidal currents and Stokes return flow. When the location was less than the resonance length, the tidal currents and Stokes return flow were most reduced due to the loss of tidal prism, the dead-end channel, and the shift from mixed to standing tides. The discharge interval primarily affected the river-dominated estuaries, and the tidal cycle period was an important time scale. When the interval was greater than the tidal cycle period, notable seaward discharge pulses and freshwater fronts occurred. Dams located near the mouth with large discharge interval differed the most from their pre-dam condition based on the estuarine classification. Greater discharge intervals, associated with large discharge magnitudes, resulted in scour and seaward sediment flux in the river-dominated estuaries, and the dam located near the resonance length resulted in the greatest landward tidal pumping sediment flux and deposition in the tide-dominated estuaries.

• Fisheries and Aquatic Sciences
• /
• 제26권1호
• /
• pp.24-34
• /
• 2023

Reactive oxygen species (ROS) at high concentrations induce oxidative stress, an imbalanced redox state that is a prevalent cause of neurodegenerative disorders. This study aimed to investigate the protective effect of Capsosiphon fulvescens (CF) extract on oxidative stress-induced impairment of cognitive function in models of neurodegenerative diseases. CF was extracted with subcritical water and several solvents and H₂O₂ (0.25 mM) or aluminum chloride (AlCl₃; 25 µM) as an inducer of ROS was treated in PC12 neuronal cells and zebrafish larvae. All statistical analyses were performed using one-way analysis of variance and Dunnett's test using GraphPad Prism. H₂O₂ and AlCl₃ were found to significantly induce ROS production in PC12 neuronal cells and zebrafish larvae. In addition, they strongly affected intracellular Ca²⁺ levels, antioxidant enzyme activity, brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) signaling, acetylcholinesterase (AChE) activity, and hallmarks of Alzheimer's disease. However, treatment of H₂O₂-induced PC12 cells or AlCl₃-induced zebrafish larvae with CF subcritical water extract at 90℃ and CF water extract effectively regulated excessive ROS production, intracellular Ca²⁺ levels, and mRNA expression of superoxide dismutase, glutathione peroxide, glycogen synthase kinase-3 beta, β-amyloid, tau, AChE, BDNF, and TrkB. Our study suggested that CF extracts can be a potential source of nutraceuticals that can improve the impairment of cognitive function and synaptic plasticity by regulating ROS generation in neurodegenerative diseases.