• Title/Summary/Keyword: primary root

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Clonal Propagation in Commiphora Wightii (Arnott.) Bhandari

  • Mishra, Dhruv Kumar;Kumar, Devendra
    • Journal of Forest and Environmental Science
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    • v.30 no.2
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    • pp.218-225
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    • 2014
  • Studies were carried out to standardize and develop a suitable macro-propagation technology for large scale production of superior clonal stock through stem cuttings in Commiphora wightii Arnott (Bhandari), a data deficient medicinal plant of arid region. For the purpose, three experiments were conducted. The first experiment was tried to elucidate the impact of various cutting diameters (0.50-0.75 cm, 0.75-1.00 cm, 1.00-1.50 cm, and >1.50 cm) in combination with varying growing conditions (sunlight, shade house and mist chamber) on shoot sprouting and rooting without using exogenous plant growth regulators. Cutting diameter (size 0.75-1.00 cm) in mist chamber has shown maximum sprouting (90.00%) and rooting (73.33%), primary root (6.67) and secondary root (16.67) followed by 1.00-1.51 cm in mist chamber. Minimum sprouting (40.00%), rooting (33.33%), number of shoot (1.33), primary root (1.00) and number of secondary root (1.00) was recorded in cutting diameter (size >1.50 cm) in sunlight. Second experiment was performed to find out optimum growth regulator concentration of rooting hormone (100, 200, 500 and 1000 ppm) of Indole-3-acetic acid (IAA) and Indole-3-butyric Acid (IBA) on adventitious root formation on cuttings diameter (size 0.25-0.50 cm) in comparison to control. Maximum rooting percentage (93.33%) was recorded in 200 ppm followed by 500 ppm (86.66%) of IBA as compared to control, which showed only 60 per cent sprouting. Third experiment was performed with newly formed juvenile micro-cuttings treated with varying concentrations of IAA and IBA. The juvenile cuttings (size 6-10 cm, basal dia <0.25 cm) were selected as micro-cuttings. The cuttings treated with IBA (500 ppm) showed 64.30% rooting as compared to other treatments. Results of above experiments indicate that cuttings (size 0.75-1.00 cm dia) may be developed in mist chamber for better performance. While using heavier cuttings, no growth promoting hormones is required however; growth regulator 200 ppm concentration of IBA rooting hormone was observed optimum for promoting macro-propagation in stem cuttings of lower diameter class (0.25-0.50 cm).

Eruption guidance for impacted canine (임상가를 위한 특집 2 - 매복견치의 맹출유도)

  • Lee, Nan-Young
    • The Journal of the Korean dental association
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    • v.50 no.6
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    • pp.312-321
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    • 2012
  • Permanent canine are very important teeth in point of function as well as esthetic. Therefore impaction of canines is a frequently encountered clinical problems. The incidence of impaction ranges between 1-3%. The cause of canine impaction can be result of ectopic position of tooth germ, defect of dental follicle, early loss or delayed exfoliation primary canine, space problem, supernumarary teeth, cyst, odontoma, Impaction frequently involves further complication such as root resorption of adjacent teeth, cyst formation and migration of the neighboring teeth. Various treatment modalities include extraction of primary canine, surgical opening and orthodontic traction, autotransplantation, surgical extraction of impacted canine.

Varietal Differences in Root Characteristics of Paddy Rice (수도 근부특성의 품종간 차이)

  • Je-Cheon Chae;Jong-Hoon Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.1
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    • pp.8-13
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    • 1980
  • It was observed that the variety Suweon 258 has a good root system for nutrient and water absorption compared to variety Jinheung, because of larger secondary and tertiary roots inducting long and narrow adventitious roots in spite of less primary roots in number and shorter average length of primary roots.

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Histopathological Observation of Three Types of Root Resorption Surface in Maxillary Primary Incisors (상악 유절치의 3가지 유형의 치근 흡수면에 관한 병리조직학적 관찰)

  • Na, Hyejin;Son, Hyoju;Song, Jeseon;Kim, Seongoh;Lee, Jaeho;Choi, Hyungjun;Choi, Byungjai
    • Journal of the korean academy of Pediatric Dentistry
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    • v.44 no.3
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    • pp.289-297
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    • 2017
  • Local and general factors have been attributed to root resorption occurred by injuries such as trauma and dental caries that affect periodontal ligament or dental pulp tissue. Pathologic root resorption is different from physiologic root resorption in terms of resorption pattern such as micromorphology of resorption fossae and types of observed cells. Microscopic morphologies and histologic features of physiologic and pathologic root resorption surface of maxillary primary central incisors resulting from trauma and periapical inflammation were observed by scanning electron microscope and light microscope. The morphology of physiologic resorption lacunae was small and oval or circular shape with regularities. The morphology of pathologic resorption lacunae was large and polygonal shape with irregularities compared with the physiologic resorption lacunae. Multinucleated giant cells and mononuclear cells were closely attached to the physiologic and pathologic resorption lacunae, whereas several kinds of mesenchymal cells with numerous inflammatory cells were found in the areas adjacent to the pathologic resorption surface. Compensating cementum formation took place along some of the areas of physiologic and pathologic resorption area resulting from trauma, but could not be observed on pathologic resorption area resulting from periapical inflammation.

Experimental study on vibration serviceability of cold-formed thin-walled steel floor

  • Bin Chen;Liang Cao;Faming Lu;Y. Frank Chen
    • Steel and Composite Structures
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    • v.46 no.4
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    • pp.577-589
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    • 2023
  • In this study, on-site testing was carried out to investigate the vibration performance of a cold-formed thin-walled steel floor system. Ambient vibration, walking excitation (single and double persons), and impulsive excitation (heel-drop and jumping) were considered to capture the primary vibration parameters (natural frequencies, damping ratios, and mode shapes) and vertical acceleration response. Meanwhile, to discuss the influence of cement fiberboard on structural vibration, the primary vibration parameters were compared between the systems with and without the installation of cement fiberboard. Based on the experimental analysis, the cold-formed thin-walled steel floor possesses high frequency (> 10 Hz) and damping (> 2%); the installed cement fiberboard mainly increases the mass of floor system without effectively increasing the floor stiffness and may reduce the effects of primary vibration parameters on acceleration response; and the human-structure interaction should be considered when analyzing the vibration serviceability. The comparison of the experimental results with those in the AISC Design Guide indicates that the cold-formed thin-walled steel floor exhibits acceptable vibration serviceability. A crest factor 𝛽rp (ratio of peak to root-mean-square accelerations) is proposed to determine the root-mean-square acceleration for convenience.

COMPLICATIONS OF SODIUM HYPOCHLORITE DURING RE-ENDODONTIC TREATMENT OF MAXILLARY PRIMARY CENTRAL INCISOR : A CASE REPORT (상악 유중절치 재근관 치료에서 차아염소산나트륨의 합병증에 대한 증례 보고)

  • Hong, So-Yi;Kim, Jin-Woo;Kim, Ji-Youn;Mah, Yon-Joo;Ahn, Byung-Duk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.39 no.2
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    • pp.186-191
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    • 2012
  • The sodium hypochlorite is widely used in endodontic treatment. While it is effective solution for disinfection of root canal system, complications during canal irrigation are rarely reported, especially in primary teeth. This report demonstrates that sodium hypochlorite extruding through the root apex might cause severe complications. A 4-year-old female patient was referred from local dental clinic to the emergency room for the management of sudden facial swelling and pain during re-endodontic treatment of the maxillary primary central incisor using sodium hypochlorite. The patient was given systemic steroids, antihistamines, antibiotics and analgesics and the maxillary primary central incisor was extracted to prevent secondary infection. Swelling began to subside after 2 days. A negative result was obtained from skin patch test with sodium hypochlorite. Thorough care must be taken in primary teeth to prevent the inadvertent injection of sodium hypochlorite to periapical tissues during root canal irrigation. When adverse reaction occurs, proper management such as analgesia, cold compression and adequate medication should be done.

Community Structure, Phytomass, and Primary Productivity in Thuja orientalis Stands on Limestone Area

  • Kwak, Young-Se;Lee, Choong-Il
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.3 no.3
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    • pp.189-196
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    • 1999
  • The community structure, phytomass, and primary productivity in Thuja orientalis stands on a limestone area located in Maepo-up, Chungbuk province in Korea were estimated quantitatively. Seven species including a small proportion of Quercus dentata were identified in the tree layer, 26 species including Ulmus macrocarpa in the shrub layer, and 79 species including Carex lnceolata in the herb layer of the Thuja stands. The vertical distribution of the fine root phytomass exhibited a power functional decrease relative to the soil depth. The seasonal changes in the fine root phytomass at a soil depth of 5 cm were closely related to the pecipitation in the study area. The productivity of the stand of stems, branches, leaves, and roots were 10.72, 0.82, 0.45 and 6.46 ton DM. $ha^{-1}$ .$yr^{-1}$, respectively. The Thuja stand had a high foliage(25%) and low rate of production per unit of foliage. The annual turnover rate of the fine roots int he Thuja stand was 6.71 $yr^{-1}$. The net primary production of the overstory including the understory was estimated at 19.48 ton DM.$ha^{-1}$.$yr^{-1}$ including an underground section of 6.46 ton DM.$ha^{-1}$.$yr^{-1}$(33%). The allocation ratio of net production to root was lower in the limestone Thuja communities than at the nearby non-limestone ones, whereas the production efficiency to leaf weight was higher in the limestone communities. These results would seem to indicate that the limited production capacity is due to the calcium toxicity and low availability of iron and phosphorus in a limestone soil with a high pH, calcium, and bicarbonate content with a strategy for survival in a hostile habitat.

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PULP TREATMENT OF PRIMARY TEETH USING A COMBINATION OF ANTIBIOTICS (항생제를 사용한 유치의 치수치료)

  • Kim, Dae-Eop
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.1
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    • pp.126-131
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    • 2005
  • The purpose of this study was to observe the potentiality of a mixture of antibiotics to relieve the endodontic and periapical infections in primary teeth. A mixture of metronidazole, ciprofloxacin, and minocyclin in distilled water or in a root canal sealer were used to disinfect the infected endodontic and periapical lesions. For application of this mixture, medication cavities were prepared at orifices of root canals by using the $Fissurotomy^{(R)}$ burs(S S White, USA). The cavities and bottom of pulp chamber were filled with antibiotics mixture. In this cases, clinical symptoms were improved following a single application of that mixture. Gingival swelling, pain, and fistulae were disappeared within a week. More long term evaluation will be needed. Although there were no reports of side effects, care should be taken if children are sensitive to antibiotics. Within the limitations of this case, the mixture of 3 antibiotics provided an excellent improvement in treatment of infected primary teeth under the physiologic root resorption.

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Establishment of Hertwig's Epithelial Root Sheath/Epithelial Rests of Malassez Cell Line from Human Periodontium

  • Nam, Hyun;Kim, Ji-Hye;Kim, Jae-Won;Seo, Byoung-Moo;Park, Joo-Cheol;Kim, Jung-Wook;Lee, Gene
    • Molecules and Cells
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    • v.37 no.7
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    • pp.562-567
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    • 2014
  • Human Hertwig's epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cells are epithelial remnants of teeth residing in the periodontium. Although the functional roles of HERS/ERM cells have yet to be elucidated, they are a unique epithelial cell population in adult teeth and are reported to have stem cell characteristics. Therefore, HERS/ERM cells might play a role as an epithelial component for the repair or regeneration of dental hard tissues; however, they are very rare population in periodontium and the primary isolation of them is considered to be difficult. To overcome these problems, we immortalized primary HERS/ERM cells isolated from human periodontium using SV40 large T antigen (SV40 LT) and performed a characterization of the immortalized cell line. Primary HERS/ERM cells could not be maintained for more than 6 passages; however, immortalized HERS/ERM cells were maintained for more than 20 passages. There were no differences in the morphological and immunophenotypic characteristics of HERS/ERM cells and immortalized HERS/ERM cells. The expression of epithelial stem cell and embryonic stem cell markers was maintained in immortalized HERS/ERM cells. Moreover, immortalized HERS/ERM cells could acquire mesenchymal phenotypes through the epithelial-mesenchymal transition via TGF-${\beta}1$. In conclusion, we established an immortalized human HERS/ERM cell line with SV40 LT and expect this cell line to contribute to the understanding of the functional roles of HERS/ERM cells and the tissue engineering of teeth.