Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Establishment of Hertwig's Epithelial Root Sheath/Epithelial Rests of Malassez Cell Line from Human Periodontium

  • Nam, Hyun (Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Kim, Ji-Hye (Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Kim, Jae-Won (Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Seo, Byoung-Moo (Department of Oral and Maxillofacial Surgery, Seoul National University) ;
  • Park, Joo-Cheol (Department of Oral Histology-Developmental Biology, Seoul National University) ;
  • Kim, Jung-Wook (Department of Pediatric Dentistry, Seoul National University) ;
  • Lee, Gene (Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University)
  • Received : 2014.06.05
  • Accepted : 2014.06.30
  • Published : 2014.07.31
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Abstract

Human Hertwig's epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cells are epithelial remnants of teeth residing in the periodontium. Although the functional roles of HERS/ERM cells have yet to be elucidated, they are a unique epithelial cell population in adult teeth and are reported to have stem cell characteristics. Therefore, HERS/ERM cells might play a role as an epithelial component for the repair or regeneration of dental hard tissues; however, they are very rare population in periodontium and the primary isolation of them is considered to be difficult. To overcome these problems, we immortalized primary HERS/ERM cells isolated from human periodontium using SV40 large T antigen (SV40 LT) and performed a characterization of the immortalized cell line. Primary HERS/ERM cells could not be maintained for more than 6 passages; however, immortalized HERS/ERM cells were maintained for more than 20 passages. There were no differences in the morphological and immunophenotypic characteristics of HERS/ERM cells and immortalized HERS/ERM cells. The expression of epithelial stem cell and embryonic stem cell markers was maintained in immortalized HERS/ERM cells. Moreover, immortalized HERS/ERM cells could acquire mesenchymal phenotypes through the epithelial-mesenchymal transition via TGF-${\beta}1$. In conclusion, we established an immortalized human HERS/ERM cell line with SV40 LT and expect this cell line to contribute to the understanding of the functional roles of HERS/ERM cells and the tissue engineering of teeth.

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References

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