• Development and Reproduction
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• v.4 no.2
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• pp.181-186
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• 2000

The expression of glucosamine-6-phosphate deaminase (GNPDA) was examined in mouse ovary from neonate to aduit. In western blot, band of Mr. 31 kDa antigen sharply increased 2 weeks after birth onward. In irmmunostaining of the adult ovary, GNPDA expression was constitutive in the theca and interstitial cells. However, expression in the granulosa cells was different according to folliculogenesis. Cytoplasm of the oocyte of some primary follicle showed positive signal but not in the antral follicle. Granulosa cells of antral follicles showed no visible sign of GNPDA expression. In the corpora lutea, the signal intensity in granulosaluteal cells increased according to luteal development and became the highest in the luteolytic phase. In summary, the differential expression of GNPDA was found in follicle cells according to folliculogenesis. It suggests that GNPDA might be involved in tissue remodeling in mouse ovary.

• Toxicological Research
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• v.29 no.3
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• pp.203-209
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• 2013

A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of ${\varepsilon}$-acetamidocaproic acid (AACA), the primary metabolite of zinc acexamate (ZAC), in rat plasma by using normetanephrine as an internal standard. Sample preparation involved protein precipitation using methanol. Separation was achieved on a Gemini-NX $C_{18}$ column ($150mm{\times}2.0mm$, i.d., 3 ${\mu}m$ particle size) using a mixture of 0.1% formic acid-water : acetonitrile (80 : 20, v/v) as the mobile phase at a flow rate of 200 ${\mu}l/min$. Quantification was performed on a triple quadrupole mass spectrometer employing electrospray ionization and operating in multiple reaction monitoring (MRM) and positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of AACA were linear over the concentration range of 20~5000 ng/ml in rat plasma. The coefficient of variation and relative error at four QC levels were ranged from 1.0% to 5.8% and from -8.4% to 6.6%, respectively. The present method was successfully applied for estimating the pharmacokinetic parameters of AACA following intravenous or oral administration of ZAC to rats.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.05a
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• pp.65-69
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• 2003

The effects of microstructural features on the fracture behaviors, including impact, high-cycle fatigue, fatigue and crack propagation, of thixoformed 357-T5 (Al-7%Si-0.6%Mg) alloy were examined. The resistance to impact and high-cycle fatigueof thixoformed 357-T5 tended to improve greatly with increasing solid volume fraction. An almost three-fold increase in impact energy value was, for example, observed with increasing solid volume fraction from 59 to 70%. The improvement in both impact and fatigue properties of thixoformed 357-75 with increasing solid volume fraction in the present study appeared to be related to the magnitude of stress concentration at the interface between primary and eutectic phase, by which the fracture process was largely influenced. Based on the fractographic and micrographic observations, the mechanism associated with the beneficial effect of high solid volume fraction in thixoformed 357-T5 alloy was discussed.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.19 no.6
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• pp.100-108
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• 2005

In this paper deals with the frequency control of the HVDC scheme linking Haenam to Cheju Island. The primary aim of the study is to develop and evaluate a new frequency control that can be employed without having to utilise the existing Synchronous Compensators(Gas Turbines). Transient condition studies are performed utilising the detailed control strategies for the HVDC link, implemented in PSCAD/EMTDC. Study cases are completed involving synchronous compensators trip and load ripping events and study plots presented. It is demonstrated that the existing frequency measurement can be replaced by one derived from the AC network alone, incorporated into a new frequency control algorithm and gives effective frequency control and dynamic performance.

• Microbiology and Biotechnology Letters
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• v.19 no.2
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• pp.135-139
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• 1991

Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30$^{\circ}C$, 250rpm, 48hour of culture and $(NH_4)_2HPO_4$ as nitrogen source.

• Journal of Power System Engineering
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• v.7 no.2
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• pp.29-35
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• 2003

The monitoring and diagnostics of the rotating machinery have been received considerable attention for many years. The objectives are to classify the machinery condition and to find out the cause of abnormal condition. This paper describes a classification method of diagnosing the small reciprocating compressor for refrigerators using the artificial neural network and the wavelet transform. In order to extract salient features, the wavelet transform are used from primary noise signals. Since the wavelet transform decomposes raw time-waveform signals into two respective parts in the time space and frequency domain, more and better features can be obtained easier than time-waveform analysis. In the training phase for classification, self-organizing feature map(SOFM) and learning vector quantization(LVQ) are applied, and the accuracies of them ate compared with each other. This paper is focused on the development of an advanced signal classifier to automatize the vibration signal pattern recognition. This method is verified by small reciprocating compressors, for refrigerator and normal and abnormal conditions are classified with high flexibility and reliability.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2001.10a
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• pp.38-42
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• 2001

A complete quantitative understanding of DT has been difficult because the process represents such a broad field of research. The experimental measurement of tool force is a single area of DT which still covers a wide range of possibilities. There are numerous parameters of the process which affect cutting forces. There are also many turnable materials of current interest. To obtain information toward a better understanding of the process, a few cutting parameters and materials were selected for detail study. It was decided that free-oxygen copper and 6061-T6 alloy aluminum would be the primary test materials. There are materials which other workers have also used because of there wide use in reflective applications. The experimental phase of the research project began by designing tests to isolate certain cutting parameters. The parameters chosen to study were those that affected the cross-sectional area of the uncut chip. The specific parameters which cause this area to vary are the depth of cut and infeed per revolution, or feedrates. Other parameter such a tool nose radius and surface roughness were investigated as they became relevant to the research.

• 한국연소학회:학술대회논문집
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• 2000.12a
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• pp.217-228
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• 2000

Circulating Fluidized Bed Combustor(CFBC) has been used for the incineration of waste sewage sludge and for the power generation. In this study hydrodynamic characteristics of two phase flow have been studied in a riser section of CFBC. A lab-scale riser is designed and SiC (Geldart type B) is used for solid particles. Experiments are performed by controlling the fluidization parameters including superficial velocity and secondary air to primary air ratio for determination of solid holdup profiles in the riser. Superficial velocities of each fluidization regime are well agreed with results predicted by a theoretical model. The results show that the axial solid holdup distributions calculated by measuring differential static pressures in the riser are found to show a basic profile described by a simple exponential function. Our flow regime during experiments mainly belongs to fast fluidization regime for particle size of 300${\mu}m$. As the SA/PA ratio increases, solid holdup in the lower dense region of the riser increases.

• Reproductive and Developmental Biology
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• v.38 no.1
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• pp.21-34
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• 2014

The majority of early embryonic mortality in pregnancy occurs during the peri-implantation stage, suggesting that this period is important for conceptus viability and the establishment of pregnancy. Successful establishment of pregnancy in all mammalian species depends on the orchestrated molecular events that transpire at the conceptus-uterine interface during the peri-implantation period. This maternal-conceptus interaction is especially crucial in pigs because in them non-invasive epitheliochorial placentation occurs, in which the pre-implantation phase is prolonged. During the pre-implantation period, conceptus survival and the establishment of pregnancy are known to depend on the developing conceptus receiving an adequate supply of histotroph, which contains a wide range of nutrients and growth factors. Evidence links growth factors including epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), vascular endothelial growth factor (VEGF), and colony-stimulating factor 2 (CSF2) to embryogenesis or implantation in various mammalian species; however, in the case of pig, little is known about such functions of these growth factors, especially their regulatory mechanisms at the maternal-conceptus interface. Our research group has presented evidence for promising growth factors affecting cellular activities of primary porcine trophectoderm (pTr) cells, and we have identified potential intracellular signaling pathways responsible for the activities induced by these factors. Therefore, this review focuses on promising growth factors at the maternal-conceptus interface regulating the development of the porcine conceptus and playing pivotal roles in implantation events during early pregnancy in pigs.

• Applied Microscopy
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• v.26 no.3
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• pp.267-275
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• 1996

The internal ultrastructural changes of germ cells and external morphology of spermatozoon during the spermatogenesis in the rockfish, Sebastes inermis were studied using transmission and scanning electron microscope. The testis is seminiferous tubule type in internal structure. Seminiferous tubule consist of many cyst which contain numerous germ cells in same developmental stage. Spermatogonium contained a large nucleus with single nucleolus in interphase. Primary spermatocyte identified by the presence of synaptonemal complex in nucleus and the contained a number of mitochondria, endoplasmic reticula and Golgi bodies in cytoplasm. The nucleoplasm of secondary spermatocyte was more concentrated than that of the previous phase. Spermatids were more condensed in nucleus and cytoplasm, and show the long-spherical shape. In the cytoplasm of spermatid mitochondria located to lower portion of the nucleus and Golgi bodies located to upper portion, but proacrosomal granule is not appeared. The spermatozoon consist of the head and tail. No acrosome could be found in the head. The cytoplasmic collar of posterior part in sperm head contained mitochondria which surrounded axial filament. The well developed axonemal lateral fins were identified in sperm flagellum, and the axial filament of the flagellum consist of nine pairs of peripheral microtubules and one pair of central microtubules.