• 한국환경농학회지
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• 제22권2호
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• pp.94-99
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• 2003

본 연구의 목적은 랫드 간세포 일차배양에서 카드뮴에 의해 유발된 세포독성 및 지질과산화에 대한 셀레늄의 항산화 및 간보호 효과를 조사하기 위함이다. 이를 위해 2개의 실험을 수행하였다. 실험 1에서는, 1, 10, 50, 100 및 $50\;{\mu}M$의 다양한 농도의 카드뮴으로 6시간 동안 간세포를 일차 배양하였다. 간세포 생존율과 지질과산화는 각각 MTT 방법과 TBARS 방법으로 측정하였다. 항산화 효과와 간보호 효과는 각각 GOT와 GSH-Px 활성을 측정함으로써 결정하였다. 카드뮴은 농도 의존적으로 세포 생존율을 감소시켰으며 지질과산화는 증가시켰다. 세포 생존율과 지질과산화 간에 유의적인 음의 상관관계(r=-0.943, p<0.01)가 관찰되었다. 카드뮴은 $50\;{\mu}M$ 농도에서 GOT의 활성을 증가시켰으나 GSH-Px의 활성은 감소시켰다. 실험 2에서는 셀레늄이 카드뮴에 의해 유발된 독성 및 지질과산화에 대한 효과를 연구하기 위해 $100\;{\mu}M$의 카드뮴과 0.01, 0.1 및 1 ppm의 다양한 농도의 셀레늄으로 6시간 동안 간세포 일차 배양하였다. 세포 생존율과 GSH-Px의 활성은 1 ppm의 셀레늄에 의해 증가되었다. 반면에, 지질과산화와 GOT의 활성은 0.1 ppm의 셀레늄에 의해 감소되었다. 이러한 연구 결과는 셀레늄이 카드뮴에 대한 항산화 및 보호 효과를 나타내 보이고 있다.

• 한국주거학회논문집
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• 제21권6호
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• pp.19-29
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• 2010

Vernacular houses in Southeast Asia are basically post and beam structures raised on pillars, with gabled roofs. They were designed and built by the people themselves to meet specific needs, accommodating the values, economies and ways of life of cultures that produce them. Their forms and housing cultures are very various according to the country and the region. But based on the common history and culture, the vernacular houses in Southeast definitely have commonality. The purpose of this study is to analyse the commonality and diversity of the vernacular houses in Southeast Asia in the aspect of two different perspectives. One is the climate perspective, for which the analysis was made on the primary characteristics such as the climate, material, structure of the vernacular houses. The other one is the social-cultural perspective which try to find the religion, belief, life style and social and family relationship which controlled the forms of the housing under the surface. As a result, this study summarise and find the outstanding and unique meaning and definition of the vernacular houses in Southeast Asia.

• 복식
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• 제61권3호
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• pp.1-19
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• 2011

In the process of modernization from the traditional Korean Han-bok to today's modernized fashion. The Republic of Korea received the most influences form the United States among other western countries. The U. S. A. was the primary country who helped to release S. Korea from Japanese colonization in 1945, and provided the most economic and military aids after the Korean War in 1950. The purpose of this case study is to find out the traits of Americanization in modern Korean fashion through sociocultural evidences and reference pictures. The Americanization can be defined as the U. S. A. influencing cultures of other countries, and sometimes resulted in the substitution of a given culture with American culture. Americanization generally is a part of westernization. The analogous terms for Americanization, are Coca-colonization, McDonaldization, Starbuckslization, and Hollywoodization. The theories related to Americanization are theory of economic determination, global system theory, conspiracy theory, modernization theory, and theory of dependence. However, Americanization of Korea cannot be explained in one single theory, but only can be explained as a hybrid of two cultures. There also is a critical viewpoint of de-Americanization. Americanization of Korea were studied in three important chronicles, a period of modernization (1945-1950s), a period of industrialization & popularization(1960s-1970s), and a period of consumerization(1980s-present). The traits of Americanization in the process of modernizing Korean fashion, were found as simplicity, functionality, popularity(kitsch & mimicry), anti-traditional ethics(unchastity & skin revealing), and materialism(lavishness & trends).

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제27권5호
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• pp.453-456
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• 2001

Background: Autogenous alveolar bone cell transplantation may be suitable for tissue engineering for alveolar bone reconstruction. This study aimed to isolate human alveolar bone-derived cells (HABDCs) and to evaluate the ability of collagen gels to support HABDC proliferation and differentiation for human alveolar bone tissue engineering applications. Method: Cultures of primary HABDCs were established from alveolar bone chips obtained from 10 persons undergoing tooth extraction. These cells were expanded in vitro until passage 3 and used for the in vitro characterization of HABDCs and the in vitro analysis of collagen gels for alveolar bone tissue engineering. Results: Of the 10 attempts made to obtain HABDC cultures, eight were successful. HABDCs expressed the osteoblastic phenotype characterized by alkaline phosphatase activity, osteocalcin expression and the mineralization of the extracellular matrix in vitro. When seeded on collagen gels, HABDCs penetrated into the collagen gel matrices and proliferated inside the gels. Significantly, when HABDCs were embedded into the gels, collagen fibers and mineralization were produced within the gels. Conclusion: This study demonstrates the feasibility of using cultured HABDCs and collagen gels for human alveolar bone tissue engineering applications.

• 한국가축번식학회지
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• 제20권3호
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• pp.259-269
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• 1996

To establish whether or not androgens is responsible for the induction of vitellogenin(Vg) synthesis and secretion, primary hepatocytes prepared from immature eels were used. The results are follows: 1. Eel hepatocytes were prepared using a collagenase perfusion technique. The isolated cells attached efficiently to fibronectin-coated dishes and subsequently formed monolayers in serum-free medium. These cultures maintained in medium for 10 days with minimal cell loss. 2. Estradiol-17$\beta$(E2) alone was insufficient to induce Vg synthesis. The combination of E2 with methyltestosterone(MT) markedly stimulated Vg synthesis. High vg production occurred in MT concentration from 10-6~10-5M in the presence of E2 (10-6M). Testosterone and androsterone were also effective, but progesterone was not effective in inducing Vg synthesis. Neither MT alone nor testosterone and androsterone alone had any effect on Vg synthesis. 3. E2-primed hepatocytes showed Vg synthesis in both media with and without hormones 1 day after culture. In the cultures with the vehicle, MT, or progesterone, the rate of synthesis seemed to decrease with time. But the combination of E2 and MT showed an intense increase in Vg synthesis. Hepatocytes isolated from E2-primed eels also required androgens for continuating of Vg synthesis. 4. These results demonstrate that androgens act together with E2 in synthesis and secretion of eel Vg.

• 약학회지
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• 제44권5호
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• pp.448-454
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• 2000

Microglia, brain resident macrophages, play a central role in the inflammatory responses of the brain and are activated in brain injuries and several neurodegenerative diseases such as Alzheimer's and Parkinson's disease, thereby aggravating the course of these diseases. In this study, the effects of plantderived compounds such as curcumin or gingerol on the microglial activation were examined. Microglial cultures were prepared from 2~3 week mixed primary glial cultures obtained from the cerebral cortex of 1~2 day old rats and identified by immunocytochemistry using microglial-specific antibody OX-42. Microglia were activated by lipopolysaccharide (LPS) and interferon-${\gamma}$ (IFN-${\gamma}$) and the effect of curcumin or 6-gingerol on the microglial activation was examined. Specific parameters measured to monitor microglial activation were nitric oxide (NO), prostaglandin E$_2$(PGE$_2$) and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) release. Curcumin (1~10 $\mu$M) inhibited NO release induced by LPS and IFN-${\gamma}$ in a dose-dependent manner whereas 6-gingerol (2~20 $\mu$M) did not have any effect on LPS/IFN-${\gamma}$-induced NO release. The levels of PGE$_2$and TNF-$\alpha$ induced by LPS and IFN-${\gamma}$ were also inhibited by 1~10 $\mu$M curcumin in a dose-dependent manner. These results showed that curcumin could modulate microglial activation.

• 동아시아경상학회지
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• 제12권2호
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• pp.43-50
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• 2024

Purpose: The present study investigates if the type of organizational culture influences the level of job-related stress. This study is significant because creating the need to explore the concept to become more informed on the best practices to promote and foster a positive culture that prioritizes workers, it could result in a healthier workforce, improved organizational performance, and success. Research design, data and methodology: Examining previous studies by other researchers was the primary research tool for this study. The organized approach of gathering data and assessing related work was vital in developing a fundamental basis for advancing knowledge on this concept while enhancing theory development and drawing more informed conclusion. Results: These four types, including the clan, adhocracy, hierarchical, and market cultures, contribute significantly in revealing four associations between the two variables, including (i) supportive culture and reduced stress, (ii) high pressure and increased stress, (iii)autonomous culture and variable stress levels, and (iv) open culture and low-stress levels. Conclusions: The literature affirms a multifaceted link between employee workplace stress and organizational culture type. The findings show that these cultures are crucial in influencing the level of stress among employees. All four types were hypothesized to have a significant relationship with job stress.

• 한국문헌정보학회지
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• 제27권
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• pp.99-124
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• 1994

This study examines the establishment of Sea-po (서포) in the opening period of Korea at the end of the 19th century. The role they played, the particular situations and their geographical characteristics are researched, The earliest sea-po was Koh-je-hong sea-po, which was established in Tae­kwang-kyo at the end of 1880s. Around 1905, a specialised book distribution system was set up with establishement of Chu-han-yung book store, Kim-sang-man book store, Jesuit book store, Tae-dong su-si, Kae-myung sea-kwan, Tong-wha seo-kwan. The owners of the seo-pos were pioneers in introducing modern culture with nationalistic consciousness, although they were primarily businessmen and their social origins varied. The primary role of seo-pos was selling a variety of books but some combined printing and publication of books as well. It seems that publication business took roots around 1908, though it is difficult to know the accurate dates of first attempts. Some sea-pas offered book rental services for poor people who could not easily afford to purchase books. A certain amount of deposit had to be paid to benefit from the services. Jesuit Bookstore in Pyungyang had a library facility with a large stock or books in the same building open to public for free. These sea-pas started mainly in Seoul and Pyungyang, which were traditional centers for economic and cultural affairs. Early introduction of foreign cultures and commercial developments in these areas contributed to the establishment of sea-pos. The sea-po which took charge of book distribution in the provinces was set up around 1906. One of the important findings of this study is that the geographical distribution of sea-po shows the following three characteristics. First is that the area such as Seoul, Taegu and Chunju were the traditional centers of publishing culture from which Pang­gak-bon(방각본) emerged. Second characteristic is Pyungyang and the surrounding Pyungbuk province, and harbors like Inchon and Pusan, which had the benefits of early spread of modem cultures. Third characteristic is Kaesung and the surrounding Hwanghae province which bridged Pyungyang and Seoul. The reception of foreign cultures stimulated the commercial spirits traditionally attributed to Kaesung to establish sea-pas most actively.

• 문화기술의 융합
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• 제5권1호
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• pp.265-273
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• 2019

본 연구는 앞으로 경험하게 될 국가적 혹은 문화적 갈등상황에서 유연한 중재자의 역할을 수행하는 인재를 육성하는 것에 그 목적이 있다. 주요한 학습 내용은 업사이클 디자인 활동을 선정하여 수업에 접근한다. 모두의 문제인 폐기물 문제를 인식시키고 그 대안으로 거론되고 있는 업사이클 디자인 활동을 거쳐 각 문화들의 대표적인 특징과 배경을 탐구하는 수업방식이다. 군포YMCA 회원 학생들을 중심으로 수업은 진행되었으며 각 문화적으로 특징적인 소재를 업사이클 디자인을 통해 제작하였다. 모두 폐기물 재료를 사용하여 페트병으로 아프리카의 기린 만들기, 나무 기둥을 활용한 몽골 텐트 만들기, 소스병을 활용한 스노우 볼 만들기, 티셔츠와 섬유로 조각보 및 러그 만들기, 현수막으로 가랜드를 만드는 활동을 진행하였다. 이를 통해 학생들은 업사이클이라는 새로운 용어를 학습하게 됨과 동시에 환경문제에 관심을 보이게 되었다. 또한 각 문화를 대표하는 상징물을 제작하는 과정에서 그 유래와 탄생 배경 등을 탐구함으로써 타 문화에 대한 이해의 폭을 넓힐 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.6973-6979
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• 2015

Background: Ovarian cancer is the third most common cause of cancer in Indian women. Despite an initial 70-80% response rate, most patients relapse within 1-2 years and develop chemoresistance. Hence, identification or repositioning of drugs to resensitise ovarian cancer cells to existing chemotherapy is needed. Traditionally immortalized cell lines have been used in research, but these may contain genetic aberrations and chromosomal abnormalities serving as poor indicators of normal cell phenotype and progression of early-stage disease. The use of primary cells, maintained for only short periods of time in vitro, may serve as the best representative for studying in vivo conditions of the tissues from which they are derived. In this study we have attempted to evaluate the effect of metformin (an antidiabetic drug) in primary ovarian cancer cells because of its promising effect in other solid tumours. Materials and Methods: Primary cultures of epithelial ovarian cancer cells established from ascitic fluid of untreated ovarian cancer patients were used. The cells were treated with metformin at doses standardized by MTT assay and its ability to induce apoptosis was studied. The cells were analysed for apoptosis and apoptosis related proteins by flow cytometry and western blotting respectively. Results: Metformin induced apoptosis in ovarian cancer cells, provoking cell cycle arrest in the G0/G1 and S phase. It induced apoptosis in ovarian cancer cells by, down-regulating Bcl-2 and up-regulating Bax expression. Conclusions: Metformin was able to induce apoptosis in primary ovarian cancer cells by modulating the expression of Bcl-2 family proteins. These data are relevant to ongoing translational research efforts exploring the chemotherapeutic potential of metformin.