• 생명과학회지
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• 제21권9호
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• pp.1323-1328
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• 2011

본 연구에서는 페놀 화합물들을 현장에서 검출하기 위해 간단하고 간편한 일회용 재조합 박테리아 바이오센서 시스템을 개발하였다. 플라즈미드 pLZCapR을 함유하는 E. coli 세포를 ${\beta}$-galactosidase 기질인 CPRG와 함께 96-well plate의 wells에 agarose로 고정하였다. 이 바이오센서는 현장에서 발색을 위한 별도의 기질을 추가하거나 불편한 기기 사용 또는 시료의 전 처리를 필요로 하지 않는다. 시료의 측정은 간단히 적은 부피(<100 ${\mu}l$)의 현장 시료를 바이오센서를 포함하는 wells에 넣고 발색을 관찰하여 측정하였다. 또한 6% DMF, 0.1% SDS 그리고 10 mM $CaCl_2$를 첨가하여 agarose 고정에 의한 화합물의 세포내 확산 제한을 감소시켜 보다 더 나은 발색을 얻을 수 있었다. 따라서 이 고정된 미생물 유래 재조합 바이오센서 시스템은 현장에서 환경오염물질들을 간단하게 확인하고 정량 하는 유용한 접근 방법이 될 것으로 사료된다.

• The Korean Journal of Physiology
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• 제29권1호
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• pp.91-102
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• 1995

The role of neurohumoral mechanisms in the regulation of cardiovascular functions and the effects of ethanol (EOH) on these mechanisms were examined in hemorrhaged conscious Wistar rats. The rats were bled at a constant rate (2 ml/kg/min) through the femoral artery until mean arterial pressure (MAP) was reduced by 30 mmHg. We studied the responses to hemorrhage 1) under normal conditions (Normal), and after pretreatments with 2) neural blockade (NB), pentolinium, 3) arginine vasopressin V1-receptor antagonist (AVPX) + NB, 4) angiotensin II ATI-receptor antagonist (AngIIX) + NB, 5) combined humoral blockade (HB), and 6) neurohumoral blockade. Intravenous administration of 30% EOH (6.3 ml/kg) attenuated the baroreceptor reflex sensitivity, and enhanced the depressor action of AngIIX. During hemorrhage, NB produced a faster fall ill MAP than Normal both in the saline and EOH groups. However, HB accelerated the rate of fall in MAP only in the EOH group. The recovery from hemorrhagic hypotension was not different between NB and Normal rats, but was attenuated in HB rats in the saline group. Under NB, AngIIX, but not AVPX, retarded the recovery rate compared with NB alone. EOH attenuated the recovery of MAP after hemorrhage in Normal rats, but completely abolished the recovery in HB rats. We conclude that 1) the maintenance of MAP during hemorrhage is mediated almost entirely by the autonomic functions, 2) angiotensin II plays an important role in the recovery from hemorrhagic hypotension, but AVP assumes little importance, 3) AVP release largely depends on the changes in blood volume, whereas renin release depends on the changes in blood pressure rather than blood volume, and 4) EOH increases the dependence of cardiovascular regulation on angiotensin II and impairs the recovery from hemorrhagic hypotension through the attenuation of autonomic functions.

• 멤브레인
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• 제4권4호
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• pp.213-220
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• 1994

정유산업 방출폐수를 재처리하여 양질의 공업용수로 활용하고자 역삼투막 및 공정으로 구성된 재활용 system을 고안하여 현장 pilot 실험을 수행하였다. 생산공정 운전상황에 따라 불규칙적으로 변하는 폐수 특성에도 불구하고 역삼투막의 경우 10~15$kg/cm^2$의 운전압력 범위내에서 96~99%정도의 비교적 높은 염배제율을 보였으며 본 실험을 통하여 얻은 재생수의 경우 냉각탑 공급용수로써의 충분한 가능성을 보여 주었다. 그러나 본 연구에서 제안 사용된 여과형 전처리 공정만으로는 충분한 처리효율을 기대하기 어려웠으며, 이는 각 여과공정법 처리수의 수질분석 결과 및 NaOH를 이용한 역삼투막 세정 결과로부터 쉽게 확인되었다.

• 한국미생물·생명공학회지
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• 제40권3호
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• pp.282-285
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• 2012

본 연구는 혐기성 소화조에서 바이오 가스 생산을 위한 바이오매스 자원으로서 Chlorella vulagaris와 Dunaliella salina의 이용능력을 확인하였다. 세포벽의 구조에 따라 전처리 후 용해성 물질의 수율이 영향을 받았는데, 이는 D. salina가 바이오 가스 생산 측면에서 C. vulgaris보다 좋은 후보라는 것을 보여준다. 혐기성 소화조에서 얻은 접종원으로부터 전처리하거나 전처리하지 않은 D. salina를 기질로서 메탄가스를 생산하는데 이용하였을 때 메탄 수율 측면에서 큰 차이가 없었다. 그러므로 D. salina는 높은 바이오매스 생산성, 단순한 전처리 필요성, 쉬운 바이오 가스전환 때문에 바이오 가스 생산을 위한 적합한 해조류 바이오매스이다.

• 한국환경농학회지
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• 제16권4호
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• pp.311-319
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• 1997

광산폐수, 산업폐수등으로 부터 Cd, Pb, Zn 및 Cu등의 중금속에 강한 내성을 지니고 있을 뿐만 아니라 균체내 중금속 축적능력이 우수한 중금속 내성 미생물 균주 Pseudomonas putida(Cd), Pseudomonas aeruginosa(Pb), Pseudomonas chlororaphis(Zn) 및 Pseudomonas stutzeri(Cu)를 각각 분리하여, 여러가지 물리화학적인 방법으로 세포를 전처리하여 세포구성성분을 인위적으로 조절한 후 세포내 중금속이온의 흡수 거동 및 조단백질 함량과 중금속 축적관계를 조사한 결과는 다음과 같다. 세포를 알카리로 전처리하였을 경우 세포내 중금속 축적은 매우 감소되었으며, 메탄올과 클로포름으로 전처리하였을 경우에는 중금속 축적에 큰 영향을 미치지 않았으나, 메탄올과 클로로포름으로 전처리한 후 다시 알카리로 재차 처리하였을 경우에는 중금속 축적이 매우 감소되었다. 전처리된 세포내 중금속 축적은 용출되지 않고 남아있는 조단백질 함량이 감소됨에 따라 더 얼마나 크게 감소되었으므로 세포 구성물질중 단백질이 중금속 축적에 중요한 역할을 하는 물질인 것으로 판단되었다.

• The Plant Pathology Journal
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• 제32권5호
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• pp.469-480
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• 2016

Bacterial wilt and grey mould in tomato plants are economically destructive bacterial and fungal diseases caused by Ralstonia solanacearum and Botrytis cinerea, respectively. Various approaches including chemical and biological controls have been attempted to arrest the tomato diseases so far. In this study, in vitro growths of bacterial R. solanacearum and fungal B. cinerea were evaluated using four different vitamins including thiamine (vitamin B1), niacin (vitamin B3), pyridoxine (vitamin B6), and menadione (vitamin K3). In planta efficacies of the four vitamin treatments on tomato protection against both diseases were also demonstrated. All four vitamins showed different in vitro antibacterial activities against R. solanacearum in dose-dependent manners. However, treatment with 2 mM thiamine was only effective in reducing bacterial wilt of detached tomato leaves without phytotoxicity under lower disease pressure ($10^6$ colony-forming unit [cfu]/ml). Treatment with the vitamins also differentially reduced in vitro conidial germination and mycelial growth of B. cinerea . The four vitamins slightly reduced the conidial germination, and thiamine, pyridoxine and menadione inhibited the mycelial growth of B. cinerea. Menadione began to drastically suppress the conidial germination and mycelial growth by 5 and 0.5 mM, respectively. Grey mould symptoms on the inoculated tomato leaves were significantly reduced by pyridoxine and menadione pretreatments one day prior to the fungal challenge inoculation. These findings suggest that disease-specific vitamin treatment will be integrated for eco-friendly management of tomato bacterial wilt and grey mould.

• 상하수도학회지
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• 제30권6호
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• pp.755-764
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• 2016

Pressure retarded osmosis (PRO) processes can be implemented on a number of water types, using different technologies and achieving various power outcomes. In this study, Sewage facility effluent was used for feed solution of PRO and synthetic NaCl water for draw solution. This study was conducted to investigate effect of water quality of pretreatment on power density and flux decline in PRO process. The results show that organic and particulate foulants have to be removed for more stable operation. Flourescence technique with EEM enables to investigate the chemical properties of aquatic organic matter by extracting spectral information. Humic/fulvic matters and soluble microbial by-products were analyzed as the most affecting factors on the PRO performance. As a result of analyzing the whole system based on the energy consumption of the unit process, specific energy consumption(SEC) of the applicable technology for PRO pre-treatment should be about $0.2kWh/m^3$ or less.

• Toxicological Research
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• 제25권1호
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• pp.29-33
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• 2009

Phellinus gilvus (PG) is a widely used mushroom for health promotion. We studied the hepatoprotective effect of the polysaccharide aqueous extract of PG (PGP) against carbon tetrachloride ($CCl_4$)-induced liver injury in rats. Sprague Dawley rats were divided into 5 groups: Normal control, $CCl_4$ control, PGP 50, 100, and 200 mg/kg + $CCl_4$. The levels of serum biochemical parameters, liver lipid peroxide and antioxidant enzymes, and histological appearances were evaluated. The $CCl_4$-induced increments of alanine aminotransferase, asparate aminotransferase, and alkaline phosphatase levels in serum were significantly decreased by PGP-pretreatments. The PGP dose-dependently decreased hepatic malondialdehyde formations in $CCl_4$-treatment groups. Hepatic antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) were elevated by PGP in $CCl_4$-treatment groups. Histopathological evaluation of liver showed that the loss of hepatocytes, fatty changes, swelling and extensive necrosis of hepatocytes in centrilobular regions of the $CCl_4$-treated rats were ameliorated by PGP pretreatment. The PGP has hepatoprotective and antioxidative effects in $CCl_4$-induced liver injury of rat.

• 원예과학기술지
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• 제31권5호
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• pp.573-579
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• 2013

본 연구는 국내육성 절화 장미의 수확 적기와 수확단계에 따른 전처리의 효과를 알아 보고자 수행하였다. 장미 절화는 3단계의 각기 다른 시기에 수확되었다. 국내육성 장미의 수확 적기는 품종에 따라 달랐다. 스탠다드 장미 'Pink Song'은 I 단계, 스프레이 장미 'Peace One'은 III 단계에서 수확 시절화 수명이 가장 길었다. 수확단계에 상관없이 대부분의 절화 수명은 2% S + $200mg{\cdot}L^{-1}$ AS + $200mg{\cdot}L^{-1}$ $Mg(NO_3)_2$ + $50mg{\cdot}L^{-1}$ $CaCl_2$ 전처리 시 무처리에 비해 연장되었고, 품질이 향상되었다. 하지만 전처리의 효과는 수확 단계에 따라 다르게 나타났다.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.2
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• pp.337-347
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• 2006

Papers made from unbleached and bleached bagasse chemimechanical pulp were chemically modified by acetylation. The effects of irradiation on unbleached and bleached also reduced papers of bagasse chemimechanical pulp before and after acetylation were investigated in this study. Chemimechanical pulp was prepared from bagasse and then bleached with hydrogen peroxide. Unbleached and hydrogen bleached pulps were reduced by Sodium borohydride in different procedures. Paper sheets were prepared from pulps and then acetylated using a technical grade of acetic anhydride. Accelerated photo-aging was run on the samples using fluorescent lamps to verify photo-stability of paper sheets before and after pretreatments. Brightness reversion (as Post-color number) and other optical properties of the paper sheets were measured. Efficient inhibition of photo-yellowing of papers made from bagasse CMP was achieved by acetylation. The acetylated unbleached CMP was noticeably photo-bleached during irradiation. Sodium borohydride reduction followed by acetylation had the same effect as acetylation alone at the same degree of reaction time and reductive treatment did not affect the yellowing rate to any great extent. The pre-reduced, acetylated unbleached papers were, however, not brightened during irradiation. Calculation done by Kubelka-Munk equation showed that reductive treatment had little effect in reducing the photo-yellowing of paper made from CMP pulp; a small stabilization effect was observed in the case of bleached CMP, while unbleached CMP was slightly more prone to discolor in the later phase of photo-reversion. The improved stability towards light may was closely related to the decrease in the phenolic hydroxyl content as a result of blocking by acetyl groups during treatment with acetic anhydride. The results support the hypothesis that phenolic hydroxyl has an important role in the process of photo-reversion of high-yield pulps. The results obtained in this study demonstrate that the acetylation of paper manufactured from peroxide bleached Bagasse CMP significantly retards light-induced discoloration. The inhibition of yellowing is connected with a decrease in the phenolic hydroxyl content of both unbleached and peroxide bleached papers.